Abstract
Probiotics play a vital role in clinical applications for the treatment of diarrhea, obesity and urinary tract infections. Phytate, an anti-nutrient, chelates essential minerals that are vital for human health. In the past few decades, research reports emphasize extensively on phytate degradation in animals. There is a growing need for finding alternate strategies of phytate utilization in human, as they are unable to produce phytase. At this juncture, probiotics can be utilized for phytase production to combat mineral deficiency in humans. The main focus of this review is on improving phosphate bioavailability by employing two approaches: supplementation of (1) fermented food products that contain probiotics and (2) recombinant phytase producing bacteria. In addition, several factors influencing phytase activity such as bacterial viability, optimal pH, substrate concentration and specificity were also discussed.
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References
FAO and WHO (2002) Joint working group report, “Guidelines for the evaluation probiotics in food”
Bohn L, Meyer AS, Rasmussen SK (2008) Phytate: impact on environment and human nutrition. A challenge for molecular breeding. J Zhejiang Univ Sci B 9:165–191. doi:10.1631/jzus.B0710640
Lei XG, Porres JM, Mullaney EJ, Brinch-Pedersen H (2007) Phytase: source, structure and application. In: Polaina J, MacCabe AP (eds) Industrial enzymes-structure, function and applications. Springer, Netherlands, pp 505–529
Shin S, Ha NC, Oh BC, Oh TK, Oh BH (2001) Enzyme mechanism and catalytic property of beta propeller phytase. Structure 9:851–858
Konietzny U, Greiner R (2004) Bacterial phytase: potential application, in vivo function and regulation of its synthesis. Braz J Microbiol 35:11–18. doi:10.1590/S1517-83822004000100002
Shewale RN, Sawale PD, Khedkar CD, Singh A (2014) Selection criteria for probiotics: a review. Int J Probiotics Prebiotics 9:17–22
Hudson LE, Anderson SE, Corbett AH, Lamb TJ (2017) Gleaning insights from fecal microbiota transplantation and probiotic studies for the rational design of combination microbial therapies. Clin Microbiol Rev 30:191–231. doi:10.1128/CMR.00049-16
Lee WJ, Lattimer LDN, Stephen S, Borum ML, Doman DB (2015) Fecal Microbiota Transplantation: a review of emerging indications beyond relapsing Clostridium difficile toxin colitis. Gastroenterol Hepatol 11:24–32
Baxter M, Colville A (2016) Adverse events in faecal microbiota transplant: a review of the literature. J Hosp Infect 92:112–127. doi:10.1016/j.jhin.2015.10.024
Shimizu M (1992) Purification and characterization of phytase from Bacillus suhtilis (natto) N–77. Biosci Biotechnol Biochem 56:1266–1269. doi:10.1271/bbb.56.1266
Hong SW, Chu IH, Chung KS (2011) Purification and biochemical characterization of thermostable phytase from newly isolated Bacillus subtilis CF92. J Korean Soc Appl Biol Chem 54:89–94. doi:10.3839/jksabc.2011.012
Lee SH, Kwon HS, Koo KT, Kang BH, Kim TY (2006) Characterization of phytase from Bacillus coagulans IDCC 1201. Korean J Microbiol Biotechnol 34:28–34
De Angelis M (2003) Phytase activity in sourdough lactic acid bacteria: purification and characterization of a phytase from Lactobacillus sanfranciscensis CB1. Int J Food Microbiol 87:259–270. doi:10.1016/S0168-1605(03)00072-2
Haros M, Bielecka M, Honke J, Sanz Y (2008) Phytate-degrading activity in lactic acid bacteria. Pol J Food Nutr Sci 58:33–40
Khodaii Z, Mehrabani Natanzi M, Naseri MH, Goudarzvand M, Dodson H, Snelling A (2013) Phytase activity of lactic acid bacteria isolated from dairy and pharmaceutical probiotic products. Int J Enterpathog 1:12–16. doi:10.17795/ijep9359
Adegbehingbe KT (2015) Effect of starter cultures on the anti-nutrient contents, minerals and viscosity of Ogwo, a fermented sorghum–Irish potato gruel. Int Food Res J 22:1247–1252
Urga K, Keshava N, Narasimha HV (1997) Effects of natural and mixed culture of lactobacilli fermentation on in vitro iron and zinc bioavailability in tef (Eragrostis tef) atmit. Bull Chem Soc Ethiop 11:101–109. doi:10.4314/bcse.v11i2.21018
Zamudio M, Gonzalez A, Medina J (2001) Lactobacillus plantarum phytase activity is due to non-specific acid phosphatase. Lett Appl Microbiol 32:181–184
Fischer MM, Egli IM, Aeberli I, Hurrell RF, Meile L (2014) Phytic acid degrading lactic acid bacteria in tef-injera fermentation. Int J Food Microbiol 190:54–60. doi:10.1016/j.ijfoodmicro.2014.08.018
Chettri R, Tamang JP (2014) Functional properties of Tungrymbai and Bekang, naturally fermented soybean foods of North East India. Int J Fermented Foods 3:87–103. doi:10.5958/2321-712X.2014.01311.8
Abriouel H, Lucas R, Ben Omar N, Valdivia E, Maqueda M, Martínez-Cañamero M, Gálvez A (2005) Enterocin AS-48RJ: a variant of enterocin AS-48 chromosomally encoded by Enterococcus faecium RJ16 isolated from food. Syst Appl Microbiol 28:383–397. doi:10.1016/j.syapm.2005.01.007
Anastasio M, Pepe O, Cirillo T, Palomba S, Blaiotta G, Villani F (2010) Selection and use of phytate-degrading LAB to improve cereal-based products by mineral solubilization during dough fermentation. J Food Sci 75:M28–M35. doi:10.1111/j.1750-3841.2009.01402.x
Reale A, Mannina L, Tremonte P, Sobolev AP, Succi M, Sorrentino E, Coppola R (2004) Phytate degradation by lactic acid bacteria and yeasts during the wholemeal dough fermentation: a 31 P NMR study. J Agric Food Chem 52:6300–6305. doi:10.1021/jf049551p
Tamang JP, Tamang B, Schillinger U, Guigas C, Holzapfel WH (2009) Functional properties of lactic acid bacteria isolated from ethnic fermented vegetables of the Himalayas. Int J Food Microbiol 135:28–33. doi:10.1016/j.ijfoodmicro.2009.07.016
Sumengen M, Dincer S, Kaya A (2012) Phytase production from Lactobacillus brevis. Turk J Biol 36:533–541. doi:10.3906/biy-1111-2
Didar Z, Khodaparast MHH (2011) Effect of different lactic acid bacteria on phytic acid content and quality of whole wheat toast bread. JFBT 1:1–10
Pulido RP, Omar NB, Abriouel H, López RL, Cañamero MM, Guyot J, Gálvez A (2007) Characterization of lactobacilli isolated from caper berry fermentations. J Appl Microbiol 102:583–590. doi:10.1111/j.1365-2672.2006.03067.x
Lavilla-Lerma L, Pérez-Pulido R, Martínez-Bueno M, Maqueda M, Valdivia E (2013) Characterization of functional, safety, and gut survival related characteristics of Lactobacillus strains isolated from farmhouse goat’s milk cheeses. Int J Food Microbiol 163:136–145. doi:10.1016/j.ijfoodmicro.2013.02.015
Roger T, Léopold TN, Funtong MCM (2015) Nutritional properties and antinutritional factors of corn paste (Kutukutu) fermented by different strains of lactic acid bacteria. Int J Food Sci. doi:10.1155/2015/502910
Chaoui A, Faid M, Belhcen R (2003) Effect of natural starters used for sourdough bread in Morocco on phytate biodegradation. East Mediterr Health J 9:141–147
Papamanoli E, Tzanetakis N, Litopoulou-Tzanetaki E, Kotzekidou P (2003) Characterization of lactic acid bacteria isolated from a Greek dry-fermented sausage in respect of their technological and probiotic properties. Meat Sci 65:859–867. doi:10.1016/S0309-1740(02)00292-9
Najafi MA, Rezaei K, Safari M, Razavi SH (2012) Use of sourdough to reduce phytic acid and improve zinc bioavailability of a traditional flat bread (Sangak) from Iran. Food Sci Biotechnol 21:51–57. doi:10.1007/s10068-012-0007-3
Sumengen M, Dincer S, Kaya A (2013) Production and characterization of phytase from Lactobacillus plantarum. Food Biotechnol 27:105–118. doi:10.1080/08905436.2013.781507
Zotta T, Ricciardi A, Parente E (2007) Enzymatic activities of lactic acid bacteria isolated from Cornetto di Matera sourdoughs. Int J Food Microbiol 115:165–172. doi:10.1016/j.ijfoodmicro.2006.10.026
Cizeikiene D, Juodeikiene G, Bartkiene E, Damasius J, Paskevicius A (2015) Phytase activity of lactic acid bacteria and their impact on the solubility of minerals from wholemeal wheat bread. Int J Food Sci Nutr 66:736–742. doi:10.3109/09637486.2015.1088939
Hayek SA, Shahbazi A, Worku M, Ibrahim SA (2014) Enzymatic activity of Lactobacillus grown in a sweet potato base medium. Br Microbiol Res J 4:509–522
Magboul AA, McSweeney PL (1999) Purification and characterization of an acid phosphatase from Lactobacillus plantarum DPC2739. Food Chem 65:15–22
Onipede GO, Banwo K, Ogunreni OR, Sanni A (2014) Influence of starter culture lactic acid bacteria on the phytic acid content of Sorghum-Ogi (an indigenous cereal gruel). Ann Food Sci Tech 15:121–134
Bawane R, Tantwai K, Rajput LPS, Kadam-Bedekar M, Kumar S, Gontia I, Tiwari S (2011) Molecular analysis of phytase gene cloned from Bacillus subtilis. Adv Stud Biol 3:103–110
García-Mantrana I, Monedero V, Haros M (2015) Reduction of phytate in soy drink by fermentation with Lactobacillus casei expressing phytases from Bifidobacteria. Plants Food Hum Nutr 70:269–274. doi:10.1007/s11130-015-0489-2
Kumar V, Sangwan P, Verma AK, Agrawal S (2014) Molecular and biochemical characteristics of recombinant β-Propeller phytase from Bacillus licheniformis strain PB-13 with potential application in Aquafeed. Appl Biochem Biotechnol 173:646–659. doi:10.1007/s12010-014-0871-9
Wua T, Chen C, Cheng Y, Ko T, Lin C, Lai H, Huang T, Liu J, Guo R (2014) Improving specific activity and thermostability of Escherichia coli phytase by structure-based rational design. J Biotechnol 175:1–6. doi:10.1016/j.jbiotec.2014.01.034
Xu W, Shao R, Wang Z, Yan X (2015) Improving the neutral phytase activity from Bacillus amyloliquefaciens DSM 1061 by site-directed mutagenesis. Appl Biochem Biotechnol 175:3184–3194. doi:10.1007/s12010-015-1495-4
Garcia-Fruitos E (2012) Lactic acid bacteria: a promising alternative for recombinant protein production. Microb Cell Fact 11:157. doi:10.1186/1475-2859-11-157
Wyszyńska A, Kobierecka P, Bardowski J, Jagusztyn-Krynicka EK (2015) Lactic acid bacteria—20 years exploring their potential as live vectors for mucosal vaccination. Appl Microbiol Biotechnol 99:2967–2977. doi:10.1007/s00253-015-6498-0
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We would like to thank Dr. V.Ramachandra Murty (Professor, Department of Biotechnology, Manipal Institute of Technology) for his timely inputs and help.
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Priyodip, P., Prakash, P.Y. & Balaji, S. Phytases of Probiotic Bacteria: Characteristics and Beneficial Aspects. Indian J Microbiol 57, 148–154 (2017). https://doi.org/10.1007/s12088-017-0647-3
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DOI: https://doi.org/10.1007/s12088-017-0647-3