Abstract
In this research, a novel electrochemical aptasensor for the detection of Penicillin in a milk sample was designed using a Penicillin aptamer as the specific recognition element and an electrospun carbon nanofiber (ECNF) mat electrodeposited gold nanoparticles (AuNPs) as the platform. Firstly, the ECNF mat electrode was fabricated by means of electrospinning and heat treatment method. Secondly, the prepared ECNF mat electrode was modified with electrodeposition of AuNPs to improve the rate of electronic transmission. Finally, a Penicillin aptamer was assembled on the modified electrode. The morphology of AuNPs/ECNF mat electrode was examined using scanning electron microscopy (SEM) equipped with energy dispersive spectroscopy (EDS). The SEM results demonstrated that the electrodeposition of AuNPs on ECNF mat electrode was successfully performed. Cyclic voltammetry (CV) was utilized to indicate the electrochemical performance of the prepared aptasensor. The CV results illustrated a high selectivity, good stability, excellent reproducibility and repeatability as well as wide linear range (1–400 ng/mL) with a low detection limit (0.6 ng/mL). Moreover, the recoveries obtained in this research were in a good agreement with those achieved using the HPLC method. Therefore, a proposed aptasensor with a good electrochemical performance and simple preparation procedure can be proposed for the detection of Penicillin antibiotic in milk samples.
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs11694-020-00684-x/MediaObjects/11694_2020_684_Fig1_HTML.jpg)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs11694-020-00684-x/MediaObjects/11694_2020_684_Fig2_HTML.png)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs11694-020-00684-x/MediaObjects/11694_2020_684_Fig3_HTML.png)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs11694-020-00684-x/MediaObjects/11694_2020_684_Fig4_HTML.png)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs11694-020-00684-x/MediaObjects/11694_2020_684_Fig5_HTML.png)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs11694-020-00684-x/MediaObjects/11694_2020_684_Fig6_HTML.png)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs11694-020-00684-x/MediaObjects/11694_2020_684_Fig7_HTML.png)
Similar content being viewed by others
References
C. Macarov, L. Tong, M. Martínez-Huélamo, M. Hermo, E. Chirila, Y. Wang, D. Barrón, J. Barbosa, Food Chem. 135, 2612 (2012)
T.M. do Prado, M.V. Foguel, L.M. Gonçalves, T.S. Maria del Pilar. Sens. Actuators B 210, 254 (2015)
G. Hui, Y. Ying, Trans. ASABE 60, 1439 (2017)
M. Bailón-Pérez, A. García-Campaña, M. del Olmo-Iruela, L. Gámiz-Gracia, C. Cruces-Blanco, J. Chromatogr. A 1216, 8355 (2009)
R.B.D. Brito, R.G. Junqueira, Braz. Arch. Biol. Technol. 49, 41 (2006)
Z. Xu, H.-Y. Wang, S.-X. Huang, Y.-L. Wei, S.-J. Yao, Y.-L. Guo, Anal. Chem. 82, 2113 (2010)
A. Junza, R. Amatya, D. Barrón, J. Barbosa, J. Chromatogr. B 879, 2601 (2011)
E.N. Evaggelopoulou, V.F. Samanidou, Food Chem. 136, 1322 (2013)
Q. Yang, L. Zhou, Y.-X. Wu, K. Zhang, Y. Cao, Y. Zhou, D. Wu, F. Hu, N. Gan, Anal. Chim. Acta 1020, 1 (2018)
Y. Zhou, C. Mahapatra, H. Chen, X. Peng, S. Ramakrishna, H.S. Nanda, Curr. Opin. Biomed. Eng. 13, 16–24 (2019)
T. Wang, H. Yin, Y. Zhang, L. Wang, Y. Du, Y. Zhuge, S. Ai, Talanta 197, 42 (2019)
S.M. Taghdisi, N.M. Danesh, M.A. Nameghi, M. Ramezani, M. Alibolandi, K. Abnous, Biosens. Bioelectron. 133, 230 (2019)
Z. **aohong, Z. Zhidong, L. **ongwei, L. Jian, H. Guohua, J. Food Meas. Charact. 11, 548 (2017)
A. Mehlhorn, P. Rahimi, Y. Joseph, Biosensors 8, 54 (2018)
J.M. **arrón, P. Yañez-Sedeño, A. González-Cortés, Electrochim. Acta 53, 5848 (2008)
Y. Li, Y.-Y. Song, C. Yang, X.-H. **a, Electrochem. Commun. 9, 981 (2007)
L. Luo, L. Zhu, Z. Wang, Bioelectrochemistry 88, 156 (2012)
X. Niu, Y. Li, J. Tang, Y. Hu, H. Zhao, M. Lan, Biosens. Bioelectron. 51, 22 (2014)
J. Zhao, W. Guo, M. Pei, F. Ding, Anal. Methods 8, 4391 (2016)
A. Mohammad-Razdari, M. Ghasemi-Varnamkhasti, Z. Izadi, A.A. Ensafi, S. Rostami, M. Siadat, Microchim. Acta 186, 372 (2019)
S.G. Kim, J.S. Lee, J. Jun, D.H. Shin, J. Jang, ACS Appl. Mater. Interfaces 8, 6602 (2016)
Z. Dong, S.J. Kennedy, Y. Wu, J. Power Sources 196, 4886 (2011)
M. Adabi, Nanomed. Res. J. 4, 247 (2019)
M. Adabi, M. Adabi, J. Dispers. Sci. Technol. (2019). https://doi.org/10.1080/01932691.2019.1678483
A. Niri, R. Faridi-Majidi, R. Saber, M. Khosravani, M. Adabi, Biointerface Res. Appl. Chem. 9, 4022 (2019)
S.R. Dhakate, A. Gupta, A. Chaudhari, J. Tawale, R.B. Mathur, Synth. Met. 161, 411 (2011)
N. Yusof, A. Ismail, J. Anal. Appl. Pyrol. 93, 1 (2012)
Z. Zhou, C. Lai, L. Zhang, Y. Qian, H. Hou, D.H. Reneker, H. Fong, Polymer 50, 2999 (2009)
C. Lai, Z. Zhou, L. Zhang, X. Wang, Q. Zhou, Y. Zhao, Y. Wang, X.-F. Wu, Z. Zhu, H. Fong, J. Power Sources 247, 134 (2014)
J.I.A. Rashid, N.A. Yusof, Sens. Biosens. Res. 16, 19 (2017)
Z. Luo, Y. Wang, X. Lu, J. Chen, F. Wei, Z. Huang, C. Zhou, Y. Duan, Anal. Chim. Acta 984, 177 (2017)
G. Rosati, M. Ravarotto, M. Scaramuzza, A. De Toni, A. Paccagnella, Sens. Actuators B 280, 280 (2019)
N. Karaseva, T. Ermolaeva, Talanta 120, 312 (2014)
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Additional information
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Ebrahimi Vafaye, S., Rahman, A., Safaeian, S. et al. An electrochemical aptasensor based on electrospun carbon nanofiber mat and gold nanoparticles for the sensitive detection of Penicillin in milk. Food Measure 15, 876–882 (2021). https://doi.org/10.1007/s11694-020-00684-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11694-020-00684-x