Abstract
Auxin polar transport genes PIN (PIN-FORMED) determine the concentration gradient of auxin in plants. To understand the relationship between the development of different tissues in Betula pendula ‘Dalecartica’, BpPIN gene expression and indole-3-acetic acid (IAA) content were analyzed using qRT-PCR, ELISA, and GUS staining. Gene expression of BpPIN genes and IAA levels in the leaves, buds, stems, xylem, and roots of B. pendula ‘Dalecartica’ and B. pendula as a control were measured. BpPIN1, BpPIN5 and BpPIN6 were upregulated during development in both species, suggesting a dominant role in the development of B. pendula ‘Dalecartica’ leaves. Moreover, BpPIN1 gene expression was positively associated with IAA levels during leaf, vein and petiole development in B. pendula ‘Dalecartica’ only. The correlation coefficient of the first three leaves was 0.69 (P = 0.04), while that of the first three petioles was 0.85 (P = 0.001). In addition, GUS staining of the pro-DR5::GUS transgenic line of cultivar was correlated with the results of BpPIN1 expression. Overall, these findings suggest that BpPIN1 is associated with the formation of lobed leaves in B. pendula ‘Dalecartica’.
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References
Adamowski M, Friml J (2015) PIN-dependent auxin transport: action, regulation, and evolution. Plant Cell 27:20–32
Ansari SA, Singh S, Rani A (2004) Inorganic salts influence IAA ionization and adventitious rhizogenesis in Pongamia pinnata. J Plant Physiol 161:17–120
Bilsborough GD, Runions A, Barkoulas M, Jenkins HW, Hasson A, Galinha C, Laufs P, Hay A, Prusinkiewicz P, Tsiantis M (2011) Model for the regulation of Arabidopsis thaliana leaf margin development. Proc Natl Acad Sci USA (PNAS) 108:3424–3429
Hay A, Barkoulas M, Tsiantis M (2006) ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis. Development 133:3955–3961
Hu QY, Zhu ZW, Peng Y, Zhao Y, Huang Y, Liu XZ, Zhang XW (2015) Cloning and characterization of auxin polar transport PIN3 gene cDNA and its promoter in Capsella bursa-pastoris. J China Agric Univ 20(1):29–36
Huang HJ (2014) Function research of AP1 in Betula platyphylla × Betula pendula. Northeast Forestry University, Harbin
Kumar P, Jharia SK, Ansari SA (2011) Change in pH regimes and adventitious root induction in semi-hardwood cuttings of Gmelina arborea Roxb. Plant Growth Regul 65:531–536
Li QJ (2015) Molecular cloning and expression analysis of PIN genes from Adiantum reniforme var.sinense y.x.lin. Huazhong Agricultural University, Wuhan
Li LC, Zhai LJ (2006) Regulation of leaf development by auxin in Arabidopsi. Chin Bull Bot 23(5):459–465
Liu XZ (2011) The study on the auxin’s distribution during the development of carpel of Capsella bursapastoris. Hunan Agricultural University, Changsha
Liu SP, Wang L, Wang JR, Xue YH, Shou HX (2009) PIN gene family in higher plants. Plant Physiol Commun 45(8):833–841
Liu FL, Kou YP, Chen XL, Gao B, Wang L, Zhao LJ (2014) Cloning and expression analysis of PIN1 and PIN2 encoding auxin efflux carriers in Rosa canina. Acta Hortic Sin 41(05):925–934
Mu HZ, Lin L, Liu GF, Jiang J (2013) Transcriptomic analysis of incised leaf-shape determination in birch. Gene 531:263–269
Okada K, Ueda J, Komaki MK, Bell CJ, Shimura Y (1991) Requirement of the auxin polar transport system in early stages of Arabidopsis floral bud formation. Plant Cell 3:677–684
Peer WA, Bandyopadhyay A, Blakeslee JJ, Makam SN, Chen RJ, Masson PH, Murphy AS (2004) Variation in expression and protein localization of the PIN family of auxin efflux facilitator proteins in flavonoid mutants with altered auxin transport in Arabidopsis thaliana. Plant Cell 16:1898–1911
Qu C, Bian XY, Jiang J, Chen S, Liu GF (2017) Leaf morphological characteristics and related gene expression characteristics analysis in Betula pendula ‘Dalecarlica’ and Betula pendula. J Bei**g For Univ 39(8):9–16
Rousi M, Pusenius J (2005) Variations in phenology and growth of European white birch (Betula pendula) clones. Tree Physiol 25:201–210
Valobra CP, James DJ (1990) In vitro shoot regeneration from leaf discs of Betula pendula ‘Dalecarlica’ EM 85. Plant Cell Tissue Organ Cult 21:51–54
Wang W, Li DQ, Yang XH, Zou Q, Zhou X, Yang J (2000) Effects of water stress on level changes of IAA and ABA in rootand shoot of different drought resistance wheat. Acta Agron Sin 26(6):737–742
Wang ZJ, Yang LW, Xu ZD, Ou ZL, Yuan HL, Ren YF, Qi LL, He ZA, Chen YS (2015) Identification and bioinformatics analysis of the PIN gene family in Jatropha curcas. Mol Plant Breed 13(05):1111–1122
Wang B, Cao HL, Huang YT, Hu YR, Qian WJ, Hao XY, Wang L, Yang YJ, Wang XC (2016) Cloning and expression analysis of auxin efflux carrier gene CsPIN3 in tea plant (Camellia sinensis). Acta Agron Sin 42(1):58–69
Yang G (2015) Function of BpGH3.5 gene in Betula platyphylla × Betula pendula. Northeast Forestry University, Harbin
Yang G, Chen S, Wang S, Liu GF, Li HY, Huang HJ, Jiang J (2015a) BpGH3.5, an early auxin-response gene, regulates root elongation in Betula platyphylla × Betula pendula. Plant Cell Tissue and Organ Cult 120(1):239–250
Yang GY, Zhao Z, Zhao YL, Zhang FJ, Gao CQ (2015b) Upstreamregulators of ThVHAc1. J Bei**g For Univ 37(12):1–6
Zhang RP (2009) Study on expression pattern of ThDHN responding to different abiotic stress and establishment of genetic transformation of Betula platyphylla. Northeast Forestry University, Harbin
Zhao YC (2009) Research on function of miR164 in Thellungiella salsuginea. Master’s Thesis of Shandong Normal University
Zhou CN, Han L, Hou CY, Metelli A, Qi LY, Tadege M, Mysore KS, Wang ZY (2011) Developmental analysis of a Medicago truncatula smooth leaf margin1 mutant reveals context-dependent effects on compound leaf development. Plant Cell 23:2106–2124
Zhu ZW, Peng Y, Zhao Y, Hu QY, Zhang XW (2013) Quantitative analysis of expression of auxin polar transport PIN genes in different tissues of Arabidopsis thaliana and Capsella bursa-pastoris. J Hunan Agric Univ (Nat Sci) 39(05):495–499
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Project funding: The work was supported by the National Natural Science Foundation of China (NSFC; Grant No. 31670673) and the 111 Project of China (Grant No. B16010).
The online version is available at http://www.springerlink.com
Corresponding editor: Tao Xu.
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Qu, C., Bian, X., Han, R. et al. Expression of BpPIN is associated with IAA levels and the formation of lobed leaves in Betula pendula ‘Dalecartica’. J. For. Res. 31, 87–97 (2020). https://doi.org/10.1007/s11676-018-0865-5
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DOI: https://doi.org/10.1007/s11676-018-0865-5