SpringerLink
Log in

Relationship between salt tolerance and nanoparticle synthesis by Williopsis saturnus NCIM 3298

  • Original Paper
  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

This work describes cell associated and extracellular synthesis of nanoparticles by the yeast, Williopsis saturnus. The yeast was able to grow in the absence and presence of sodium chloride (NaCl) and form nanoparticles in a cell associated manner. The content of melanin, a stress-associated pigment was found to be progressively greater in the presence of increasing concentrations of NaCl. With higher quantities of melanin (extracted from yeast cells grown in the presence of 4% of NaCl), smaller sized nanoparticles were obtained. This is the first report on understanding the relationship between halotolerance, production of a stress-related pigment (melanin) and synthesis of nanoparticles with antioxidant properties by using W. saturnus as a model system. The cell free extracts derived from cultures grown in the absence of NaCl were able to mediate extracellular synthesis of gold and silver nanoparticles and the biomolecule mediating nanoparticle synthesis was identified to be a glycolipid. Extracellularly synthesized gold nanoparticles displayed good catalytic activity and rapidly mediated the reduction of 4-nitrophenol to 4-aminophenol.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  • Andreishcheva EN, Isakova EP, Sidorov NN, Abramova NB, Ushakova NA, Shaposhnikovl GL, Soares MIM, Zvagilskaya RA (1999) Adaptation to salt stress in a salt-tolerant strain of the yeast Yarrowia lipolytica. Biochem (Moscow) 9:1061–1067

    Google Scholar 

  • Apte M, Girme G, Nair R, Bankar A, Kumar AR, Zinjarde S (2013) Melanin mediated synthesis of gold nanoparticles by Yarrowia lipolytica. Mater Lett 95:149–152

    Article  CAS  Google Scholar 

  • Apte M, Chaudhari P, Vaidya A, Kumar AR, Zinjarde S (2016) Application of nanoparticles derived from marine Staphylococcus lentus in sensing dichlorvos and mercury ions. Colloids Surf A 501:1–8

    Article  CAS  Google Scholar 

  • Balakumaran MD, Ramachandrana R, Balashanmugama P, Mukeshkumar DJ, Kalaichelvan PT (2016) Mycosynthesis of silver and gold nanoparticles: Optimization, characterization and antimicrobial activity against human pathogens. Microbiol Res 182:8–20

    Article  CAS  Google Scholar 

  • Bankar AV, Kumar AR, Zinjarde SS (2009) Environmental and industrial applications of Yarrowia lipolytica. Appl Microbiol Biotechnol 84:847–865

    Article  CAS  Google Scholar 

  • Bello XV, Devesa-Rey R, Cruz JM, Moldes AB (2012) Study of the synergistic effects of salinity, pH, and temperature on the surface-active properties of biosurfactants produced by Lactobacillus pentosus. J Agric Food Chem 60:1258–1265

    Article  Google Scholar 

  • Bhardwaj G, Cameotra SS, Chopra HK (2013) Biosurfactants from Fungi: A Review. J Pet Environ Biotechnol 4:6

    Article  Google Scholar 

  • Bhattacharya I, Bezawada J, Yan S, Tyagi RD (2015) Optimization and production of silver-protein conjugate as growth inhibitor. J Bionanosci 9:1–9

    Article  Google Scholar 

  • Fogarty RV, Tobin JM (1996) Fungal melanins and their interactions with metals. Enzym Microb Technol 19:311–317

    Article  CAS  Google Scholar 

  • Gangula A, Podila R, Ramakrishna M, Karnam L, Janardhana C, Rao AM (2011) Catalytic reduction of 4-nitrophenol using biogenic gold and silver nanoparticles derived from Breynia rhamnoides. Langmuir 27:1

    Article  Google Scholar 

  • Gunde-Cimerman N, Zalar P, de Hoog S, Plemenitas A (2000) Hypersaline waters in salterns—natural ecological niches for halophilic black yeasts. FEMS Microbiol Ecol 32:235–240

    CAS  Google Scholar 

  • Gupta C, Prakash D, Gupta S (2013) Functional foods enhanced with microbial antioxidants. Acad J Nutr 2:10–18

    CAS  Google Scholar 

  • Hosseini-Abari A, Emtiazi G Ghasemi SM (2013) Development of an eco-friendly approach for biogenesis of silver nanoparticles using spores of Bacillus athrophaeus. World J Microbiol Biotechnol 29:2359–2364

    Article  CAS  Google Scholar 

  • Ito H, Inouhe M, Tohoyama H, Joho M (2007) Characteristics of copper tolerance in Yarrowia lipolytica. BioMetals 20:773–780

    Article  CAS  Google Scholar 

  • Jacobson ES (2000) Pathogenic roles for fungal melanins. Clin Microbiol Rev 13:708–717

    Article  CAS  Google Scholar 

  • Jha AK, Prasad K, Prasad K (2009) A green low-cost biosynthesis of Sb2O3 nanoparticles. Biochem Eng J 43:303–306

    Article  CAS  Google Scholar 

  • Johnson M, Boese-Marrazzo DI (1980) Production and properties of heat-stable extracellular hemolysin from Pseudomonas aeruginosa. Infect Immun 29:1028–1033

    CAS  Google Scholar 

  • Kamat S, Gaikwad S, Kumar AR, Gade WN (2013) Xylitol production by Cyberlindnera (Williopsis) saturnus, a tropical mangrove yeast from xylose and corn cob hydrolysate. J App Microbiol 115:1357–1367

    Article  CAS  Google Scholar 

  • Khodashenas B, Ghorbani HR (2015) Synthesis of silver nanoparticles with different shapes. Arabian J Chem. doi:10.1016/j.arabjc.2014.12.014

    Google Scholar 

  • Kiran G, Sabu A, Selvin J (2010) Synthesis of silver nanoparticles by glycolipid biosurfactant produced from marine Brevibacterium casei MSA19. J Biotechnol 148:221–225

    Article  CAS  Google Scholar 

  • Kowshik M, Deshmukh N, Vogel W, Urban J, Kulkarni SK, Paknikar KM (2002a) Microbial synthesis of semiconductor CdS nanoparticles, their characterization, and their use in the fabrication of an ideal diode. Biotechnol Bioeng 78:583–588

    Article  CAS  Google Scholar 

  • Kowshik M, Vogel W, Urban J, Kulkarni SK, Paknikar KM (2002b) Microbial synthesis of semiconductor PbS nanocrystallites. Adv Mater 14:815–818

    Article  CAS  Google Scholar 

  • Kralj Kuncic M, Kogej T, Drobne D, Gunde-Cimerman N (2010) Morphological response of the halophilic fungal genus Wallemia to high salinity. App Env Microbiol 76:329–337

    Article  Google Scholar 

  • Kumar S, Gummadi SN (2009) Osmotic adaptation in halotolerant yeast, Debaryomyces nepalensis NCYC 3413: role of osmolytes and cation transport. Extremophiles 13:793–805

    Article  CAS  Google Scholar 

  • Meredith P, Sarna T (2006) The physical and chemical properties of eumelanin. Pigment Cell Res 19:572–594

    Article  CAS  Google Scholar 

  • Mittal AK, Kaler A, Banerjee UC (2012) Free radical scavenging and antioxidant activity of silver nanoparticles synthesized from flower extract of Rhododendron dauricum. Nano Biomed Eng 4:118–124

    Article  CAS  Google Scholar 

  • Moghaddam AB, Namvar F, Moniri M, Tahir PM, Azizi S, Mohamad R (2015) Nanoparticles biosynthesized by fungi and yeast: A review of their preparation, properties, and medical applications. Molecules 20:16540–16565

    Article  CAS  Google Scholar 

  • Mohite P, Apte M, Kumar AR, Zinjarde S (2015) Marine organisms in nanoparticle synthesis. In: Kim SK (ed) Springer handbook of marine biotechnology, Biomedical applications of marine biotechnology. Springer, Berlin Heidelberg, pp 1229–1245

    Google Scholar 

  • Mohite P, Apte M, Kumar AR, Zinjarde S (2016) Biogenic nanoparticles from Schwanniomyces occidentalis NCIM 3459: mechanistic aspects and catalytic applications. App Biochem Biotechnol 179:583–596

    Article  CAS  Google Scholar 

  • Morita T, Ishibashi Y, Fukuoka T, Imura T, Sakai H, Abe M, Kitamoto D (2011) Production of glycolipid biosurfactants, cellobiose lipids, by Cryptococcus humicola JCM 1461 and their interfacial properties. Biosci Biotechnol Biochem 75:1597–1599

    Article  CAS  Google Scholar 

  • Morita T, Fukuoka T, Imura T, Kitamoto D (2013) Accumulation of cellobiose lipids under nitrogen limiting conditions by two ustilaginomycetous yeasts, Pseudozyma aphidis and Pseudozyma hubeiensis. FEMS Yeast Res 13:44–49

    Article  CAS  Google Scholar 

  • Nair V, Sambre D, Joshi S, Bankar A, Kumar AR, Zinjarde S (2013) Yeast-derived melanin mediated synthesis of gold nanoparticles. J Bionanosci 7:159–168

    Article  CAS  Google Scholar 

  • Nghia N, Truong NNK, Thong NM, Hung NP (2012) Synthesis of nanowire-shaped silver by polyol process of sodium chloride. Int J Mater Chem 2:75–78

    Article  Google Scholar 

  • Oren A (2002) Halophilic microorganisms and their environments. Kluwer Scientific Publishers, Dordrecht

    Book  Google Scholar 

  • Pedras MS, Yu Y (2009) Salt stress induces production of melanin related metabolites in the phytopathogenic fungus Leptosphaeria maculans. Nat Prod Commun 4:53–58

    CAS  Google Scholar 

  • Peng Y, Chi ZM, Wang XH, Li J (2009) Purification and molecular characterization of exo-β-1, 3-glucanases from the marine yeast Williopsis saturnus WC91-2. App Microbiol Biotechnol 85:85–94

    Article  CAS  Google Scholar 

  • Pimprikar PS, Joshi SS, Kumar AR, Zinjarde SS, Kulkarni SK (2009) Influence of biomass and gold salt concentration on nanoparticle synthesis by the tropical marine yeast Yarrowia lipolytica NCIM 3589. Colloids Surf B 74:309–316

    Article  CAS  Google Scholar 

  • Prasad CH Srinivasulu K, Venkateswarlu P (2015) Catalytic reduction of 4-nitrophenol using biogenic silver nanoparticles derived from papaya (Carica papaya) peel extract. Ind Chem 1:104

    Google Scholar 

  • Rani HSS, Ramesh JT, Subramanian SM (2013) Production and characterization of melanin pigment from halophilic black yeast Hortaea werneckii. Int J Pharma Res Rev 2:9–17

    Google Scholar 

  • Sajjan SS, Kulkarni GB, Yaligara V, Kyoung L, Karegoudar TB (2010) Purification and physiochemical characterization of melanin pigment from Klebsiella sp. GSK. J Microbiol Biotechnol 20:1513–1520

    Article  CAS  Google Scholar 

  • Singh P, Kim YJ, Zhang D, Yang DC (2016) Biological synthesis of nanoparticles from plants and microorganisms. Trends Biotechnol 34:588–599

    Article  CAS  Google Scholar 

  • Sowani H, Mohite P, Munot H, Shouche Y, Bapat T, Kumar AR, Kulkarni M, Zinjarde S (2016a) Green synthesis of gold and silver nanoparticles by an actinomycete Gordonia amicalis HS-11: mechanistic aspects and biological application. Process Biochem 51:374–383

    Article  CAS  Google Scholar 

  • Sowani H, Mohite P, Damle S, Kulkarni M, Zinjarde S (2016b) Carotenoid stabilized gold and silver nanoparticles derived from the actinomycete Gordonia amicalis HS-11 as effective free radical scavengers. Enzym Microb Technol 95:164–173

    Article  CAS  Google Scholar 

  • Sprott GD, Larocque S, Cadotte N, Dicaire CJ, McGee M, Brisson JR (2003) Novel polar lipids of halophilic eubacterium Planococcus H8 and archaeon Haloferax volcanii. Biochim Biophys Acta 1633:179–188

    Article  CAS  Google Scholar 

  • Tresner HD, Hayes JA (1971) Sodium chloride tolerance of terrestrial fungi. Appl Microbiol 22:210–213

    CAS  Google Scholar 

  • Vadkertiova R, Slavikova E (2006) Metal tolerance of yeasts isolated from water, soil and plant environments. J Basic Microbiol 46:145–152

    Article  CAS  Google Scholar 

  • Vora JU, Jain NK, Modi HA (2014) Extraction, characterization and application studies of red pigment of halophile Serratia marcescens KH1R KM035849 isolated from Kharaghoda soil. Int J Pure App Biosci 2:160–168

    Google Scholar 

  • Wadhwani SA, Shedbalkar UU, Singh R, Karve MS, Chopade BA (2014) Novel polyhedral gold nanoparticles: green synthesis, optimization and characterization by environmental isolate of Acinetobacter sp.SW30. World J Microbiol Biotechnol 30:2723–2731

    Article  CAS  Google Scholar 

  • Wu S, Yan S, Qi W, Huang R, Cui J, Su R, He Z (2015) Green synthesis of gold nanoparticles using aspartame and their catalytic activity for p-nitrophenol reduction. Nanoscale Res Lett 10:213

    Article  Google Scholar 

  • Zhang X, Qu Y, Shen W, Wang J, Li H, Zhang Z, Li S, Zhou J (2016) Biogenic synthesis of gold nanoparticles by yeast Magnusiomyces ingens LH-F1 for catalytic reduction of nitrophenols. Colloid Surf A 497:280–285

    Article  CAS  Google Scholar 

Download references

Funding

This work was supported by funds from University Grants Commission under University with Potential for excellence, Phase II. Pallavi Mohite thanks Council of Scientific and Industrial Research (CSIR), New Delhi, India for senior research fellowship.

Author information

Authors and Affiliations

  1. Institute of Bioinformatics and Biotechnology, Savitribai Phule Pune University, Pune, Maharashtra, 411007, India

    Pallavi Mohite, Ameeta Ravi Kumar & Smita Zinjarde

  2. Department of Microbiology, Savitribai Phule Pune University, Pune, Maharashtra, 411007, India

    Smita Zinjarde

Authors
  1. Pallavi Mohite
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ameeta Ravi Kumar
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Smita Zinjarde
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Smita Zinjarde.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 294 KB)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Mohite, P., Kumar, A.R. & Zinjarde, S. Relationship between salt tolerance and nanoparticle synthesis by Williopsis saturnus NCIM 3298. World J Microbiol Biotechnol 33, 163 (2017). https://doi.org/10.1007/s11274-017-2329-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s11274-017-2329-z

Keywords

Search

Navigation