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Molecular Cloning and Analysis of a Cytosolic Hsp70 Gene from Enteromorpha prolifera (Ulvophyceae, Chlorophyta)

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Abstract

Heat shock protein 70 (Hsp70) is one of the important members of heat shock protein (Hsp) families and plays essential roles in folding nascent protein, translocation, refolding denatured protein, protein degradation, adverse stress resistance, and so on. In this study, homologous cloning coupled with the rapid amplification of cDNA ends was used to clone full-length cytosolic heat shock protein 70 of Enteromorpha prolifera (designed as EPHsp70). Bioinformatics was used to analyze structural feature, homologous relationship, and phylogenetic position of EPHsp70. The full length of EPHsp70 cDNA was 2,265 bp, with a 5′ untranslated region of 65 bp, a 3′ untranslated region of 217 bp, and an open-reading frame of 1,983 bp encoding a polypeptide of 660 amino acids with an estimated molecular weight of 71.39 kDa and an estimated isoelectric point of 5.03. EPHsp70 had five degenerate repeats of tetrapeptide GGMP and three typical Hsp70 signature motifs. The C-terminus amino acid sequence of cytosolic EPHsp70 was EEVD, and the conservation of EPHsp70 of N-terminus was higher than that of C-terminus. The homology between EPHsp70 and the cytosolic Hsp70s of other algae and land plants was more than 70%.

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Acknowledgments

This work was supported by the project of the Shandong agriculture seedstock breeding and the project of healthful and highly effective cultivation technology development and demonstration of crab and shrimp at encircled pond in the East China Sea area for financial assistance (2007BAD43B08). The authors thank anonymous reviewers for the critical comments and suggestions for the manuscript.

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Authors and Affiliations

  1. Zhejiang Marine Development Research Institute, Zhoushan, 316100, People’s Republic of China

    Wandong Fu

  2. Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, People’s Republic of China

    Wandong Fu, Jianting Yao, Shenhui Yu, Fuli Liu & Delin Duan

  3. College of Chemistry, Chemical Engineering and Environmental Science, Qingdao University, Qingdao, 266071, People’s Republic of China

    Li Shuai

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  1. Wandong Fu
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  2. Li Shuai
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Correspondence to Delin Duan.

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Fu, W., Shuai, L., Yao, J. et al. Molecular Cloning and Analysis of a Cytosolic Hsp70 Gene from Enteromorpha prolifera (Ulvophyceae, Chlorophyta). Plant Mol Biol Rep 28, 430–437 (2010). https://doi.org/10.1007/s11105-009-0170-8

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