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Differing patterns of hsp70 gene expression in invasive and native kelp species: evidence for acclimation-induced variation

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Abstract

Temperature is one of the primary factors determining the geographic boundaries of seaweeds. Thus, investigations of how seaweeds cope with temperature stress and what affects their ability for range expansion are particularly important when studying invasive species. In physiological ecology, an established index of thermotolerance is the up-regulation of heat shock genes and subsequent synthesis of heat shock proteins (Hsps). The goal of this study was to examine the up-regulation of the hsp70 gene to assess physiological tolerances of the Eastern Pacific invasive kelp, Undaria pinnatifida, as compared to a potentially competing native kelp, Egregia menziesii. Individuals of both species were collected from six known Undaria invasion sites on the west coast of North America and held in the laboratory for 1–2 weeks for acclimation at 10°C. Samples were then heat shocked at 7 temperatures for 1 h. RNA was extracted, reverse transcribed, and amplified in quantitative PCR reactions to determine relative amounts of hsp70 transcript. Results indicate that the native Egregia may be locally adapted to different thermal regimes across latitude, while the invasive Undaria populations exhibit similar expression profiles across latitude but differ by habitat.

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Acknowledgements

This study was funded primarily by an EPA STAR grant S.K.H. Additional funds were obtained from a PISCO grant to G.E.H. The authors wish to acknowledge the following individuals who made this study possible: Steve Lonhart of the Monterey Bay National Marine Sanctuary for identification of Undaria locations in Monterey Harbor, and Harbor Master Steve Pryor for access to Monterey Harbor; Marla Ranelletti for identification of Undaria sites in Santa Barbara Harbor; Rachel Woodfield for identification of Undaria sites in Los Angeles Harbor; Erin Maloney of Moss Landing Marine Laboratories for identification of Undaria sites in San Diego Bay; Kathy Ann Miller of the University of California Berkeley for Undaria collection from Catalina Island; and Eugenio Carpizo of UABC and Elizabeth Hoaglund of UCSB for assistance with Undaria collection from Todos Santos Island in Baja California, Mexico. We appreciate temperature data for Todos Santos Island shared by Julio Palleiro of CICESE. This is contribution number 243 from PISCO, the Partnership for Interdisciplinary Studies of Coastal Oceans funded primarily by the Gordon and Betty Moore Foundation and David and Lucile Packard Foundation.

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  1. Department of Ecology, Evolution, and Marine Biology, University of California, Santa Barbara, CA, 93106, USA

    Sarah K. Henkel & Gretchen E. Hofmann

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  1. Sarah K. Henkel
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Henkel, S.K., Hofmann, G.E. Differing patterns of hsp70 gene expression in invasive and native kelp species: evidence for acclimation-induced variation. J Appl Phycol 20, 915–924 (2008). https://doi.org/10.1007/s10811-007-9275-3

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