SpringerLink
Log in

Apoptosis and proliferation: correlation with p53 in astrocytic tumours

  • Laboratory Investigation
  • Published:
Journal of Neuro-Oncology Aims and scope Submit manuscript

Abstract

Apoptosis and cell proliferation occur simultaneously in tumour tissue with tumour suppressor gene, p53 being one of the key players in the complex relationship between these two key phenomena. We, as well as several other groups, have earlier demonstrated the association of p53 immunopositivity with increased degree of cell proliferation in astrocytic tumours. Here we have studied the extent of apoptosis in 62 primary human astrocytic tumours [25 Diffuse Astrocytoma (DA), 9 Anaplastic Astrocytoma (AA) and 28 Glioblastoma multiforme (GBM)] in relation to tumour grade, proliferative status and p53 protein expression. Apoptosis was measured by the TUNEL assay while, cell proliferation (MIB-1 index) and p53 protein immunoreactivity were evaluated by immunohistochemical staining using MIB-1 and DO-1 monoclonal antibodies respectively. The apoptotic index (AI) was greater in GBM than in AA or DA, and more in tumours with p53 immunopositivity than in those without. The most striking observation was the strong correlation between Apoptotic index (AI) and proliferation index (PI) in p53 negative GBM (r=0.766, P < 0.005). However this was not observed in p53 +ve GBM or in low grade DA either p53 positive or negative. Taking p53 negativity in IHC as evidence of a functional gene/protein, this extends the link between proliferation and apoptosis, hitherto observed only in cultured cells with functional p53, to a subset of solid tumours.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (Germany)

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. CG Ferreira M Ep** FAE Kruyt G Gaiccone (2002) ArticleTitleApoptosis: target of cancer therapy Clin Cancer Res 8 2024–2034 Occurrence Handle1:CAS:528:DC%2BD38XmtVWit7w%3D Occurrence Handle12114400

    CAS  PubMed  Google Scholar 

  2. B Amirlak WT Couldwell (2003) ArticleTitleApoptosis in glioma cells: review and analysis of techniques used for study with focus on the laser scanning cytometer J Neuro-Oncol 63 129–145 Occurrence Handle10.1023/A:1023906316524

    Article  Google Scholar 

  3. G Majno I Joris (1995) ArticleTitleApotosis, oncosis and necrosis. An overview of cell death Am J Pathol 146 3–15 Occurrence Handle1:STN:280:ByqC28%2FhtFw%3D Occurrence Handle7856735

    CAS  PubMed  Google Scholar 

  4. SH Kaufmann GJ Gores (2002) ArticleTitleApoptosis in cancer: cause and cure Bioessays 22 1007–1017 Occurrence Handle10.1002/1521-1878(200011)22:11<1007::AID-BIES7>3.0.CO;2-4

    Article  Google Scholar 

  5. I Pommepuy F Terro B Petit Trimoreau V Bellet S Robert J Hugon F Labrousse C Yardin A Brefeldin (2003) ArticleTitleInduces apoptosis and cell cycle blockade in glioblastoma cell lines Oncology 64 IssueID4 459–467 Occurrence Handle10.1159/000070307 Occurrence Handle1:CAS:528:DC%2BD3sXktVyhur0%3D Occurrence Handle12759546

    Article  CAS  PubMed  Google Scholar 

  6. RS Carrol J Zhang BW Chauncey K Chantziara MP Frosch PM Black (1997) ArticleTitleApoptosis in astrocytic neoplasm Acta Neurochir 139 845–850 Occurrence Handle10.1007/BF01411402

    Article  Google Scholar 

  7. DW Ellison PV Steart KC Gatter RO Weller (1995) ArticleTitleApoptosis in cerebral astrocytic tumour and its relationship to expression of the bcl-2 and p53 proteins Neuropathol Appl Neurobiol 21 352–361 Occurrence Handle1:CAS:528:DyaK2MXos1Glurk%3D Occurrence Handle7494604

    CAS  PubMed  Google Scholar 

  8. MA Heesters J Kousdstaal KG Go WM Molenaar (1999) ArticleTitleAnalysis of proliferation and apoptosis in brain gliomas: prognostic and clinical value J Neurooncol 44 255–266 Occurrence Handle10.1023/A:1006398613605 Occurrence Handle1:STN:280:DC%2BD3c7otlWntQ%3D%3D Occurrence Handle10720205

    Article  CAS  PubMed  Google Scholar 

  9. R Kordek M Hironishi PP Liberski R Yanagihara CD Gajdusek (1996) ArticleTitleApoptosis in glial tumours as determined by in situ non-radioactive labeling of DNA breaks Acta Neuro Pathol 91 112–116 Occurrence Handle10.1007/s004010050400 Occurrence Handle1:CAS:528:DyaK28XhtFyksrs%3D

    Article  CAS  Google Scholar 

  10. S Nakagawa T Shiraishi S Kihara K Tabuchi (1995) ArticleTitleDetection of DNA strand breaks associated with apoptosis in human brain tumours Virchows Arch 427 175–179 Occurrence Handle10.1007/BF00196523 Occurrence Handle1:CAS:528:DyaK2MXpvF2it70%3D Occurrence Handle7582248

    Article  CAS  PubMed  Google Scholar 

  11. M Nakamura N Konishi S Tsunoda Y Hiasa T Tsuzuki T Invi T Sakaki (1997) ArticleTitleRetinoblastom protein expression and MIB-1 correlate with survival of patients with malignant astrocytomas Cancer 80 242–249 Occurrence Handle10.1002/(SICI)1097-0142(19970715)80:2<242::AID-CNCR12>3.0.CO;2-U Occurrence Handle1:STN:280:ByiA28%2FgtV0%3D Occurrence Handle9217037

    Article  CAS  PubMed  Google Scholar 

  12. Y Takekawa T Sawada I Sakurai (1999) ArticleTitleExpression of apoptosis and its related protein in astrocytic tumours Brain Tumour Pathol 16 11–16 Occurrence Handle1:STN:280:DC%2BD3c%2FgtVaiug%3D%3D

    CAS  Google Scholar 

  13. A Nakamizo T Inamura K Ikezaki K Yoshimoto S Inoha M Mizoguch T Amano M Fukui (2002) ArticleTitleEnhanced apoptosis in pilocytic astrocytoma: a comparative study of apoptosis and proliferation in astrocytic tumours J Neuro-Oncology 57 105–114 Occurrence Handle10.1023/A:1015705305540

    Article  Google Scholar 

  14. AM Heesters J Koudstaal G Gwan WM Molenaar (2002) ArticleTitleProliferation and apoptosis in long term surviving low grade gliomas in relation to radiotherapy J Neuro-Oncol 58 157–165 Occurrence Handle10.1023/A:1016046125698

    Article  Google Scholar 

  15. MJ Verstegen DT Leenstra H Ij lst-Keizers DA Bosch (2002) ArticleTitleProliferation and apoptosis – related proteins in intracranial ependymomas: an immunohistochemical analysis J Neurooncol 56 21–28 Occurrence Handle10.1023/A:1014471714058 Occurrence Handle1:STN:280:DC%2BD383htl2gtA%3D%3D Occurrence Handle11949823

    Article  CAS  PubMed  Google Scholar 

  16. D Schiffer R Ferracini P Cavalla (2001) ArticleTitleHeterogeneity of apoptotic pathways and C. Jun/JNK expression in malignant gliomas Anticancer Res 21 2531–2535 Occurrence Handle1:CAS:528:DC%2BD3MXovVOqtL0%3D Occurrence Handle11724318

    CAS  PubMed  Google Scholar 

  17. TD Tlsty (2002) ArticleTitleFunctions of p53 supress critical consequences of damage and repair in the initiation of cancer Cell 2 IssueID1 2–4 Occurrence Handle10.1016/S1534-5807(01)00112-5 Occurrence Handle1:CAS:528:DC%2BD38XlslOqs70%3D

    Article  CAS  Google Scholar 

  18. SM Morris (2002) ArticleTitleA role of p53 in the frequency and mechanism of mutation Mutat. Res. 511 45–62 Occurrence Handle10.1016/S1383-5742(01)00075-8 Occurrence Handle1:CAS:528:DC%2BD38**tFWrsr8%3D Occurrence Handle11906841

    Article  CAS  PubMed  Google Scholar 

  19. C Prives PA Hall (1999) ArticleTitleThe p53 pathway J Pathol 187 112–126 Occurrence Handle10.1002/(SICI)1096-9896(199901)187:1<112::AID-PATH250>3.3.CO;2-V Occurrence Handle1:CAS:528:DyaK1MXmvVKrtQ%3D%3D Occurrence Handle10341712

    Article  CAS  PubMed  Google Scholar 

  20. AJ Levine (1997) ArticleTitlep53, the cellular gatekeeper for growth and division Cell 88 323–333 Occurrence Handle10.1016/S0092-8674(00)81871-1 Occurrence Handle1:CAS:528:DyaK2sXhtFahtLg%3D Occurrence Handle9039259

    Article  CAS  PubMed  Google Scholar 

  21. DA Carson JM Ribeiro (1993) ArticleTitleApoptosis and disease Lancet 34 1251–1254 Occurrence Handle10.1016/0140-6736(93)91154-E

    Article  Google Scholar 

  22. BW Stewart (1994) ArticleTitleMechanisms of apoptosis: integration of genetic biochemical and cellular indicators J Natl Cancer Inst 86 1286–1296 Occurrence Handle1:CAS:528:DyaK2MXht1agt78%3D Occurrence Handle8064887

    CAS  PubMed  Google Scholar 

  23. AH Wyllie (1993) ArticleTitleApoptosis (The 1992 Frnk Rose memorial Lecture) Cancer 67 205–208 Occurrence Handle1:STN:280:ByyC2MbpvVE%3D

    CAS  Google Scholar 

  24. P Hainaut M Hollstein (2000) ArticleTitlep53 and human cancer: the first ten thousand mutations Adv. Cancer Res 77 81–137 Occurrence Handle1:CAS:528:DC%2BD3cXltl2ktQ%3D%3D Occurrence Handle10549356

    CAS  PubMed  Google Scholar 

  25. MB Kastan O Oneykwere D Sidransky B Vogelstein RW Craig (1991) ArticleTitleParticipation of p53 protein in the cellular response to DNA damage Cancer Res 51 6304–6311 Occurrence Handle1:CAS:528:DyaK38XhvFSltg%3D%3D Occurrence Handle1933891

    CAS  PubMed  Google Scholar 

  26. SW Lowe T Jacks DE Housman HE Ruley (1994) ArticleTitleAbrogation of oncogenes associated apoptosis allows transformation of p53 deficient cells Proc Natl Acad Sci 91 2026–2030 Occurrence Handle1:CAS:528:DyaK2c**tFCjurw%3D Occurrence Handle8134344

    CAS  PubMed  Google Scholar 

  27. G Fulki N Ishii EG Van Heir (1998) ArticleTitlep53 and brain tumours – from gene mutations to gene therapy Brain Pathol 8 599–613 Occurrence Handle9804370

    PubMed  Google Scholar 

  28. Cavenee WK, Funhari FB, Nagane M, Huang HJS, Newcomb EW, Bigner DD, Weller M, et al.: Diffusely infiltrating astrocytomas. In: Kleihunes P, Cavenee WK (eds) Pathology and Genetics of tumours of the Nervous System. IARC Press, Lyon pp. 10–21

  29. EW Newcomb WJ Madonia S Pisharody FF Lang N Koslow DC Miller (1993) ArticleTitleA correlative study of p53 protein alteration and p53 gene mutation in glioblastoma multiforme Brain Pathol 3 229–235 Occurrence Handle1:CAS:528:DyaK2cXhs1Shs7Y%3D Occurrence Handle8293182

    CAS  PubMed  Google Scholar 

  30. M Nozaki M Tada H Kobayashi CL Zhang Y Sawamura H Abe N Ishii EG VanMeir (1999) ArticleTitleRoles of functional loss of p53 and other genes in astrocytoma tumourigenesis and progression Neuro-Oncol 1 IssueID2 124–137 Occurrence Handle10.1215/S1522851798000179 Occurrence Handle1:STN:280:DC%2BD3MrgtFCrsw%3D%3D Occurrence Handle11550308

    Article  CAS  PubMed  Google Scholar 

  31. C Sarkar AM Talte MC Sharma VS Mehta (2002) ArticleTitleRecurrent astrocytic tumours- a study of p53 immunoreactivity and malignant progression Br J Neurosurg 16 335–342 Occurrence Handle10.1080/02688697021000007588 Occurrence Handle1:STN:280:DC%2BD38njtFGjuw%3D%3D Occurrence Handle12389885

    Article  CAS  PubMed  Google Scholar 

  32. P Birner M Piribauer I Fischer B Gatterbauer C Marosi K Ungersbock K Rossler H Budka JA Hainfellner (2002) ArticleTitlePrognostic relevance of p53 protein expression in glioblastoma Oncol Rep 9 IssueID4 703–707 Occurrence Handle1:CAS:528:DC%2BD38XltlGku7w%3D Occurrence Handle12066196

    CAS  PubMed  Google Scholar 

  33. P Fei WS El-Deiry (2003) ArticleTitlep53 and radiation responses Oncogene 22 5774–5783 Occurrence Handle10.1038/sj.onc.1206677 Occurrence Handle1:CAS:528:DC%2BD3sXmslemt7o%3D Occurrence Handle12947385

    Article  CAS  PubMed  Google Scholar 

  34. FF Lang JM Bruner GN Fuller K Aldape MD Prados S Chang MS Bergen MW McDermott et al. (2003) ArticleTitlePhase I trial of adenovirus-mediated p53 gene therapy for recurrent glioma: biological and clinical results J Clin Oncol 21 IssueID13 2508–2518 Occurrence Handle1:CAS:528:DC%2BD2cXptlCktrc%3D Occurrence Handle12839017

    CAS  PubMed  Google Scholar 

  35. P Chattopadhyay A Rathore M Mathur C Sarkar AK Mahapatra S Sinha (1997) ArticleTitleLoss of heterozygosity of a locus on 17p13.3, independent of p53 is associated with higher grades of astrocytic tumours Oncogene 15 871–874 Occurrence Handle10.1038/sj.onc.1201238 Occurrence Handle1:CAS:528:DyaK2sXls1Krs78%3D Occurrence Handle9266974

    Article  CAS  PubMed  Google Scholar 

  36. C Sarkar P Chattopadhyay et al. (2003) ArticleTitleLoss of heterozygosity of a locus in the chromosomal region 17p13.3 is associated with increased cell proliferation in astrocytic tumours Cancer Gen Cytogen 144 156–164 Occurrence Handle10.1016/S0165-4608(02)00937-8 Occurrence Handle1:CAS:528:DC%2BD3sXltF2htr8%3D

    Article  CAS  Google Scholar 

  37. C Sarkar A Rathore P Chattopadhyay AK Mahapatra S Sinha (2000) ArticleTitleRole of 17p13.3 chromosomal region in determining p53 protein immunopositivity in human astrocytic tumours Pathol 32 84–88 Occurrence Handle10.1080/003130200104286 Occurrence Handle1:CAS:528:DC%2BD3cXktVOls7k%3D

    Article  CAS  Google Scholar 

  38. Kleihues P, Burger PC, Collins VP, Newcomb EV, Ohgaki H, Cavenee WK, Kleihues P, Cavenee WK: (eds) Tumours of the nervous system. World health Organization. Classification Tumours Eds: IARC press, Lyon, 2000

  39. JP Steinbach M Weller (2002) ArticleTitleMechanism of apoptosis in central nervous system tumours: application to theory Curr Neurol Neurosci Rep 2 IssueID3 246–253 Occurrence Handle11937003

    PubMed  Google Scholar 

  40. J Ehrmann SuffixJr. et al. (2000) ArticleTitleThe expression of apoptosis related proteins and the apoptotic rate in glial tumours of the brain Neoplasma 47 151–155 Occurrence Handle1:CAS:528:DC%2BD3cXlvFyktr4%3D Occurrence Handle11043837

    CAS  PubMed  Google Scholar 

  41. D Casper et al. (2000) ArticleTitleAcetaminophen selectively reduces glioma cell growth and reduces radiosensitivity in culture J Neuro-Oncol 46 215–229 Occurrence Handle10.1023/A:1006492423666 Occurrence Handle1:STN:280:DC%2BD3M%2Fjt1Chuw%3D%3D

    Article  CAS  Google Scholar 

  42. C Chao S Saito J Kang et al. (2000) ArticleTitlep53 transcriptional activity is essential for p53 dependent apoptosis following Dna damage Embo J 19 IssueID18 4967–4975 Occurrence Handle10.1093/emboj/19.18.4967 Occurrence Handle1:CAS:528:DC%2BD3cXntVartLw%3D Occurrence Handle10990460

    Article  CAS  PubMed  Google Scholar 

  43. GS Jimenez M Nister JM Stommel et al. (2000) ArticleTitleA tranactivation-deficient mouse model provides insight into Trp53 regulation and function Nat Genet 26 IssueID10 37–43 Occurrence Handle10.1038/79152 Occurrence Handle1:CAS:528:DC%2BD3cXmsVKkurc%3D Occurrence Handle10973245

    Article  CAS  PubMed  Google Scholar 

  44. K Polyak Y **a JL Sweier et al. (1997) ArticleTitleA model for p53 induced apoptosis Nature 389 300–305 Occurrence Handle10.1038/38525 Occurrence Handle1:CAS:528:DyaK2sXmtFGnsr4%3D Occurrence Handle9305847

    Article  CAS  PubMed  Google Scholar 

  45. Y Haupt S Rowan E Shaulian K Vousden et al. (1995) ArticleTitleInduction of apoptosis in HeLa cells by trans-activation-deficient p53 Gene Dev 9 2170–2183 Occurrence Handle1:CAS:528:DyaK2MXotVOnu7k%3D Occurrence Handle7657168

    CAS  PubMed  Google Scholar 

  46. C Koumenis R Alarcon E Hammond et al. (2001) ArticleTitleRegulation of p53 by Hypoxia: dissociation of transcriptional repression and apoptosis from p53 dependent transactivation Mol Cell Biol 21 1297–1300 Occurrence Handle10.1128/MCB.21.4.1297-1310.2001 Occurrence Handle1:CAS:528:DC%2BD3MXovVCmsg%3D%3D Occurrence Handle11158315

    Article  CAS  PubMed  Google Scholar 

  47. C Friesen I Herr PH Krammer KM Debatin (1996) ArticleTitleInvolvement of the CD95 (AP0-1/FAS) receptor/ ligand system in drug induced apoptosis in leukemia cells Nat Med 2 574–577 Occurrence Handle10.1038/nm0596-574 Occurrence Handle1:CAS:528:DyaK28**slyhs7s%3D Occurrence Handle8616718

    Article  CAS  PubMed  Google Scholar 

  48. T Miyashita S Krajewski M Krajewska et al. (1990) ArticleTitleTumour suppressor p53 is a regulator of bcl-2 and bax gene expression in vitro and in vivo Oncogene 9 1799–1805

    Google Scholar 

  49. S Owen W Zhang JC Cusack et al. (1995) ArticleTitleWild type human p53 and a temperature sensitive mutant induce Fas/APO-1 expression Mol Cell Biol 15 3032–3040 Occurrence Handle7539102

    PubMed  Google Scholar 

  50. Mihara M, Erster S, Zaika A, Petrenko O, Chiteen den T, Pancoska P, Mool M: (2003). Mol. Cell. On line

  51. E Bedner et al. (2000) ArticleTitleTranslocation of Bax to mitochondria during apoptosis measured by laser scanning cytometry Cytometry 41 83–88 Occurrence Handle10.1002/1097-0320(20001001)41:2<83::AID-CYTO1>3.0.CO;2-R Occurrence Handle1:CAS:528:DC%2BD3cXnsFWnsbc%3D Occurrence Handle11002262

    Article  CAS  PubMed  Google Scholar 

  52. H Ikemoto et al. (2000) ArticleTitleCalphostin C-mediated translocation and integration of Bax into mitochondria induces cytochrome C release before mitochondrial dysfunction Cell Death Differ 7 511–520 Occurrence Handle10.1038/sj.cdd.4400682 Occurrence Handle1:CAS:528:DC%2BD3cXktFCltLw%3D Occurrence Handle10822274

    Article  CAS  PubMed  Google Scholar 

  53. P Marchetti et al. (1996) ArticleTitleMitochondrial permeability transition is a central coordinating event of apoptosis J Exp med 184 1155–1160 Occurrence Handle10.1084/jem.184.3.1155 Occurrence Handle1:CAS:528:DyaK28XlsFartLY%3D Occurrence Handle9064332

    Article  CAS  PubMed  Google Scholar 

  54. EA Slee et al. (1999) ArticleTitleOrdering the cytochrome C-initiated caspases cascade; hierarchical activation of caspases –2, −3,−6,−7 –8 and –10 in a caspase –9- dependent manner J cell Biol 144 282–292 Occurrence Handle10.1083/jcb.144.2.281

    Article  Google Scholar 

  55. T Glaser et al. (2001) ArticleTitleDeath receptor-independent cytochrome C release and caspase activation mediate thymidine Kinase plus ganciclovir – mediated cytocoxicity in LN-18 and LN 229 human malignant glioma cells Gene Ther 8 469–476 Occurrence Handle10.1038/sj.gt.3301415 Occurrence Handle1:CAS:528:DC%2BD3M**tFaisrw%3D Occurrence Handle11313826

    Article  CAS  PubMed  Google Scholar 

  56. N Shinoura et al. (2000) ArticleTitleAdenovirus mediated Overexpression of Fas induces apoptosis of gliomas Cancer Gene Ther 7 224–232 Occurrence Handle10.1038/sj.cgt.7700110 Occurrence Handle1:CAS:528:DC%2BD3c**t1ygsL0%3D Occurrence Handle10770630

    Article  CAS  PubMed  Google Scholar 

  57. JA Kraus C Bolln HK Wolf J Neumann D Kinderman R Fimmers F Forster A Baumann U Schlegel (1994) ArticleTitleTP53 alterations and clinical outcome in low grade astrocytomas Gene Chromosome Cancer 10 143–149 Occurrence Handle1:CAS:528:DyaK2MXjsVCntQ%3D%3D

    CAS  Google Scholar 

  58. DN Louis (1994) ArticleTitleThe p53 gene and protein in human brain tumours J Neuropathol Exp Neur 53 11–21 Occurrence Handle1:CAS:528:DyaK2c**s1Kqs7o%3D

    CAS  Google Scholar 

  59. K Watanabe O Tachibana K Sata Y Yonekawa P Kleihues H Ohgaki (1996) ArticleTitleOverexpression of the EGF receptor and p53 mutations are mutually exclusive in the evolution of primary and secondary glioblastomas Brain Pathol 6 217–223 Occurrence Handle1:STN:280:ByiD3Mjms1U%3D Occurrence Handle8864278

    CAS  PubMed  Google Scholar 

  60. KH Vousden (2000) ArticleTitlep53 death star Cell 103 IssueID5 691–694 Occurrence Handle10.1016/S0092-8674(00)00171-9 Occurrence Handle1:CAS:528:DC%2BD3cXos1Snuro%3D Occurrence Handle11114324

    Article  CAS  PubMed  Google Scholar 

  61. DN Louis A won Deimling RY Chung et al. (1993) ArticleTitleComparative study of p53 gene and protein alterations in human astrocytic tumours J Neuropathol Exp Neurol 52 31–38 Occurrence Handle1:STN:280:ByyC2cjotlw%3D Occurrence Handle8381161

    CAS  PubMed  Google Scholar 

  62. L Anker et al. (1993) ArticleTitlep53 protein accumulation and gene mutations in human glioma cell lines Int J Cancer 55 982–987 Occurrence Handle1:CAS:528:DyaK2c**s1Kqu7s%3D Occurrence Handle8253536

    CAS  PubMed  Google Scholar 

  63. AM Davidoff PA Humphrey JD Iglehart JR Marks (1991) ArticleTitleGenetic basis for p53 overexpression in human breast cancer Proc Natl Acad Sci USA 88 5006–5010 Occurrence Handle1:CAS:528:DyaK3MXksVehtrc%3D Occurrence Handle2052583

    CAS  PubMed  Google Scholar 

  64. JR Marks AM Davidoff BJ Kerns et al. (1991) ArticleTitleOverexpression and mutation of p53 in epithelial ovarian cancer Cancer Res 51 2979–2984 Occurrence Handle1:STN:280:By6B3sritVE%3D Occurrence Handle2032235

    CAS  PubMed  Google Scholar 

  65. JAM Vet PP Brignuier HE Schaafsma et al. (1995) ArticleTitleComparison of p53 protein overexpression with p53 mutation in bladder cancer: clinical and biologic aspects Lab Invest 73 837–843 Occurrence Handle1:STN:280:BymC3cbht1U%3D Occurrence Handle8558845

    CAS  PubMed  Google Scholar 

  66. A Peraud WK Friedrich OD Wiestler et al. (2002) ArticleTitlePrognostic impact of p53 mutation and p53 protein overexpression in Supratentorial WHO Grade II astrocytomas and oliogoastrocytomas Clinical Cancer Res 8 1117–1124 Occurrence Handle1:CAS:528:DC%2BD38XksVSqu7g%3D

    CAS  Google Scholar 

  67. M Mizoguchi Inamura T T Shomo K Ikezaki S Inoha S Ohgami M Fukui (2002) ArticleTitleA comparative study of apoptosis and proliferation in germinoma and glioblastoma J Neuro-Oncol 2 96–102

    Google Scholar 

  68. L Sach J Loten (1993) ArticleTitleControl of programmed cell death in normal and leukemic cells: new implications for therapy Blood 82 15–81 Occurrence Handle8324219

    PubMed  Google Scholar 

  69. CB Thompson (1995) ArticleTitleApoptosis in the pathogenesis and treatment of disease Science 276 1456–1462

    Google Scholar 

  70. IM Alderson RI Castleberg GRT Harsh DN Louis JW Henson (1995) ArticleTitleHuman gliomas with wild type p53 express bcl-2 Cancer Res 55 999–1001 Occurrence Handle1:CAS:528:DyaK2MXktVGitLs%3D Occurrence Handle7867012

    CAS  PubMed  Google Scholar 

  71. G Bellomo M Pertothi F Taddei F Mirabell G finardi P Nicotera S Orrenivs (1992) ArticleTitleTumour necrosis factor alpha induces apoptosis in mammory adenocarcinoma cells by an increase in intranuclear free Ca2+ concentration and DNA fragmentation Cancer Res 52 1342–1346 Occurrence Handle1:CAS:528:DyaK38XhsV2rs7s%3D Occurrence Handle1737395

    CAS  PubMed  Google Scholar 

  72. AR Clarke CA Purdie DJ Harrison RG Morris CC Bird ML Hooper AH Wyllie (1993) ArticleTitleThymocyte apoptosis induced by p53 dependent and independent pathways Nature 362 849–852

    Google Scholar 

  73. H Symonds L Krall L Remington M Saenz-Robles S Lowe T Jacks T Vandyke (1994) ArticleTitlep53 dependent apoptosis suppress tumour growth and progression in vivo Cell 78 703–711 Occurrence Handle10.1016/0092-8674(94)90534-7 Occurrence Handle1:CAS:528:DyaK2cXlslKnsLc%3D Occurrence Handle8069917

    Article  CAS  PubMed  Google Scholar 

  74. RH Rhodes (1998) ArticleTitleBiological evaluations of biopsies from adult cerebral astrocytomas: cell-growth/cell sucide rations and their relationship to patient survival J Neuropathol Exp Neur 57 746–757 Occurrence Handle1:STN:280:DyaK1czosFCntg%3D%3D

    CAS  Google Scholar 

  75. K Dietzmann P Von Bossanyi J Sallaba E Kirches HJ Synowitz M Warich-Kirches (1996) ArticleTitleImmunohistochemically detectable p53 and mdm-2 oncoprotein expression in astrocytic gliomas and their correlation to cell proliferation Gen Diagn Pathol 141 IssueID5–6 339–344 Occurrence Handle1:STN:280:BymA2svntVI%3D Occurrence Handle8780933

    CAS  PubMed  Google Scholar 

  76. P Korkolopoulou P Christodoulou K Kouzelizs M Hadjiyannakis A Priftis G Stamoulis A Seretis E Trhomas-Tsagli (1997) ArticleTitleMDM2 and p53 expression in gliomas: a multivariate survival analysis including proliferation markers and epidermal growth factor receptor Br J Cancer 75 IssueID9 1269–1278 Occurrence Handle1:STN:280:ByiB1MbovFI%3D Occurrence Handle9155045

    CAS  PubMed  Google Scholar 

  77. T Tihan R Davis E Elowitz D Dicostanzo U Moll (2000) ArticleTitlePractical value of Ki-67 and p53 labeling indices instereotactic biopsies of diffuse and pilocytic astrocytomas Arch pathol Lab Med 124 IssueID1 108–113 Occurrence Handle1:STN:280:DC%2BD3c%2FpsVKgsQ%3D%3D Occurrence Handle10629140

    CAS  PubMed  Google Scholar 

  78. D Schiffer P Cavalla A Migheli A Chio MT Giordana S Marino A Attanasio (1995) ArticleTitleApoptosis and cell proliferation in human neuroepithelial tumours Neurosci Lett 195 81–84 Occurrence Handle10.1016/0304-3940(95)11784-T Occurrence Handle1:CAS:528:DyaK2MXntl2mu78%3D Occurrence Handle7478273

    Article  CAS  PubMed  Google Scholar 

  79. E Patsouris P Davaki N Kapranos P Davaris K Papageorglou (1996) ArticleTitleA study of apoptosis in brain tumours by in situ end labeling method Clin Neuropathol 15 337–341 Occurrence Handle1:STN:280:ByiD1M3htFE%3D Occurrence Handle8937780

    CAS  PubMed  Google Scholar 

  80. PK Lipponen S Aaltomaas (1994) ArticleTitleApoptosis in bladder cancer as related to standard prognositc factors and prognosis J Pathol 173 333–339 Occurrence Handle10.1002/path.1711730408 Occurrence Handle1:STN:280:ByqD2MvntlM%3D Occurrence Handle7965393

    Article  CAS  PubMed  Google Scholar 

  81. HH Ishii GC Gobe W Pan J YoneYama Y Ebihara (2002) ArticleTitleApoptosis and cell proliferation in the development of gastric carcinomas: association with c-myc and p53 protein expression J Gastroenterol Hepatol 17 IssueID9 966–972 Occurrence Handle10.1046/j.1440-1746.2002.02805.x Occurrence Handle1:CAS:528:DC%2BD38XotFKhsbY%3D Occurrence Handle12167117

    Article  CAS  PubMed  Google Scholar 

  82. AJ Wagner JM Kokontis N Hay (1994) ArticleTitleMyc mediated apoptosis requires wild type p53 in a manner independent of cell cycle arrest and the ability of p53 to induce p21 WAFI Genes Dev 8 2817–2830 Occurrence Handle1:CAS:528:DyaK2M**s1aisbY%3D Occurrence Handle7995520

    CAS  PubMed  Google Scholar 

  83. H Hermeking D Eick (1994) ArticleTitleMediation of c-Myc-induced apoptosis by p53 Science 265 IssueID5181 2091–3009 Occurrence Handle1:CAS:528:DyaK2cXmt1Ogurs%3D Occurrence Handle8091232

    CAS  PubMed  Google Scholar 

  84. VS Prasad RE LaFond M Zhou KA Jacobsen DG Osmond CL Sidman (1997) ArticleTitleUpregulation of endogenous p53 and induction of in vivo apoptosis in β-lineage lymphomas of E(mu)-myc transgenic mice by deregulated c-myc transgene Mol Carcinog 18 IssueID2 66–77 Occurrence Handle10.1002/(SICI)1098-2744(199702)18:2<66::AID-MC2>3.0.CO;2-O Occurrence Handle1:CAS:528:DyaK2sXhsFKgt7Y%3D Occurrence Handle9049182

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pathology, All India Institute of Medical Sciences, Ansari Nagar, 110029, New Delhi, India

    Chitra Sarkar, Asis Kumar Karak & Mehar Chand Sharma

  2. Department of Biochemistry, All India Institute of Medical Sciences(AIIMS), Ansari Nagar, 110029, New Delhi, India

    Neera Nath, Parthoprasad Chattopadhyay & Subrata Sinha

  3. Department of Neurosurgery, All India Institute of Medical Sciences (AIIMS), New Delhi, India

    Ashok Kumar Mahapatra

Authors
  1. Chitra Sarkar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Asis Kumar Karak
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Neera Nath
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mehar Chand Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ashok Kumar Mahapatra
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Parthoprasad Chattopadhyay
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Subrata Sinha
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Chitra Sarkar.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sarkar, C., Karak, A.K., Nath, N. et al. Apoptosis and proliferation: correlation with p53 in astrocytic tumours. J Neurooncol 73, 93–100 (2005). https://doi.org/10.1007/s11060-004-3015-1

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11060-004-3015-1

Keywords

Search

Navigation