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Characterization of a wheat HSP70 gene and its expression in response to stripe rust infection and abiotic stresses

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Abstract

Members of the family of 70-kD heat shock proteins (HSP70 s) play various stress-protective roles in plants. In this study, a wheat HSP70 gene was isolated from a suppression subtractive hybridization (SSH) cDNA library of wheat leaves infected by Puccinia striiformis f. sp. tritici. The gene, that was designated as TaHSC70, was predicted to encode a protein of 690 amino acids, with a molecular mass of 73.54 KDa and a pI of 5.01. Further analysis revealed the presence of a conserved signature that is characteristic for HSP70s and phylogenetic analysis demonstrated that TaHSC70 is a homolog of chloroplast HSP70s. TaHSC70 mRNA was present in leaves of both green and etiolated wheat seedlings and in stems and roots. The transcript level in roots was approximately threefold less than in leaves but light–dark treatment did not charge TaHSC70 expression. Following heat shock of wheat seedlings at 40°C, TaHSC70 expression increased in leaves of etiolated seedlings but remained stable at the same level in green seedlings. In addition, TaHSC70 was differentially expressed during an incompatible and compatible interaction with wheat-stripe rust, and there was a transient increase in expression upon treatment with methyl jasmonate (MeJA) treatment. Salicylic acid (SA), ethylene (ET) and abscisic acid (ABA) treatments had no influence on TaHSC70 expression. These results suggest that TaHSC70 plays a role in stress-related responses, and in defense responses elicited by infection with stripe rust fungus and does so via a JA-dependent signal transduction pathway.

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Acknowledgements

We thank Dr. **-Rong Xu at Purdue University for critical reading of this manuscript. This work was financially supported by the National High Technology Research and Development Program of China (2006AA10A104), the Program for Changjiang Scholars and Innovative Research Team in University, Ministry of Education of China (No. 200558), the Nature Science Foundation of China (No. 30671350), the 111 Project from Ministry of Education of China (No. B07049), the Key Project of Chinese Ministry of Education (No. 107104), the Program for Excellent Young Scholars in Northwest A&F University and the Innovative Program for the Undergraduates in Northwest A&F University.

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  1. College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling, 712100, Shaanxi, People’s Republic of China

    Ying-Hui Duan, Jun Guo, Ke Ding, Shu-Juan Wang, Hong Zhang, **-Wei Dai, Yue-Ying Chen, Li-Li Huang & Zhen-Sheng Kang

  2. Laboratory of Phytopathology, Plant Sciences Group, Wageningen University, Binnenhaven 5, 6709 PD, Wageningen, The Netherlands

    Francine Govers

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  1. Ying-Hui Duan
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Correspondence to Zhen-Sheng Kang.

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Ying-Hui Duan and Jun Guo contributed equally to this work.

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Duan, YH., Guo, J., Ding, K. et al. Characterization of a wheat HSP70 gene and its expression in response to stripe rust infection and abiotic stresses. Mol Biol Rep 38, 301–307 (2011). https://doi.org/10.1007/s11033-010-0108-0

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