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Genetic admixture of mitten crabs in the Northeast Asia hybrid zones

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Abstract

Hybridization and genetic admixture in hybrid zones promote phenotypic and genotypic adaptive changes in organisms. Hybrids between Chinese mitten crab (Eriocheir sinensis) and Japanese mitten crab (Eriocheir japonica) have been reported in the Vladivostok region of Russia. However, the direction and extent of hybridization remains unknown in Northeast Asia. In this paper, genetic variation and admixture were investigated by examining a mitochondrial NADH dehydrogenase subunit II gene (ND2) and 10 microsatellite DNA loci in mitten crabs from the Tumen River in China and Vladivostok in Russia. In addition, the adjacent distributions of E. sinensis in China and E. japonica in Japan were analyzed. The Tumen River and Vladivostok were both identified as hybrid zones of E. sinensis and E. japonica. As indicated by ND2 sequences analysis, the mitten crabs in the Tumen River and Vladivostok displayed 5–10 times higher nucleotide diversity and more mixed haplotypes than pure E. sinensis and E. japonica. Meanwhile, approximately 31.25% of the mitten crabs in Vladivostok and 30.00% in the Tumen River were identified as potential hybrids, indicating strong genetic admixture between the two mitten crabs in these hybrid zones. Our results provide quantitative evidence of genetic admixture of E. sinensis and E. japonica in Northeast Asia.

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Acknowledgements

We would like to thank Wucheng Yue for their assistance in DNA extraction and Data analysis. This work was supported by Agriculture Research System of Shanghai, China (Grant No. 201704), Shanghai Agriculture Applied Technology Development Program, China (Grant No. G2017-02-08-00-10-F00076), Shanghai Science and Technology Committee Programs (No. 16391905300; No. 13DZ2251800), Young teachers training Project of Shanghai Municipal Education Commission (A1-2039-17-0011), Doctoral Program of Shanghai Ocean University (A2-0203-00-100315), and Innovation project of Shanghai Ocean University Graduate Education (A1-0209-1500903-2).

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    1. Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, China

      Wei Kang, Shu Huang, **aowen Chen, Jun Wang & Chenghui Wang

    2. Faculty of Fisheries, Kagoshima University, Shimoarata, Kagoshima, 890-0056, Japan

      Jun Ohtomi

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    Correspondence to Chenghui Wang.

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    This study was approved by the Institutional Animal Care and Use Committee (IACUC) of the Shanghai Ocean University, Shanghai, China. All sampling procedures complied with the guidelines of IACUC on the care and use of animals for scientific purposes.

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    Handling editor: Diego Fontaneto

    Wei Kang and Shu Huang authors contributed equally to this work.

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    Kang, W., Huang, S., Chen, X. et al. Genetic admixture of mitten crabs in the Northeast Asia hybrid zones. Hydrobiologia 806, 203–214 (2018). https://doi.org/10.1007/s10750-017-3358-1

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