SpringerLink
Log in

Development of 20 microsatellite loci in the Japanese mitten crab Eriocheir japonica and cross-amplification in the Chinese mitten crab Eriocheir sinensis

  • Technical Note
  • Published:
Conservation Genetics Resources Aims and scope Submit manuscript

Abstract

The Japanese mitten crab (Eriocheir japonica) is a catadromous species endemic to East Asia with the widest distribution of all species in the mitten crab taxonomy. To date, no novel microsatellite loci for this species have been reported. Twenty polymorphic microsatellite loci were isolated, and cross-amplification was conducted in the Yangtze and Yellow River populations of Chinese mitten crab (Eriocheir sinensis). Four loci were found to deviate significantly from the Hardy–Weinberg equilibrium. Similar numbers of alleles, mean observed (H O) and expected (H E) heterozygosities, mean gene diversity, and inbreeding coefficients (F IS) were detected in the two populations of E. sinensis. We concluded that this suite of microsatellite loci was successful for cross-amplification in E. sinensis and might be useful for the assessment of population structure of the mitten crab.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (Canada)

Instant access to the full article PDF.

Institutional subscriptions

References

  • Chang YM, Liang LQ, Li SW, Ma HT, He JG, Sun XW (2006) A set of new microsatellite loci isolated from Chinese mitten crab, Eriocheir sinensis. Mol Ecol Notes 6:1237–1239

    Article  CAS  Google Scholar 

  • Excoffier L, Laval G, Schneider S (2005) ARLEQUIN version 3.0: an integrated software package for population genetics data analysis. Evol Bioinform Online 1:47–50

    CAS  PubMed  Google Scholar 

  • Goudet J (1995) FSTAT (version 1.2): a computer program to calculate F-statistics. J Hered 86(6):485–486

    Google Scholar 

  • Guo JY, Ng NK, Dai A, Ng PKL (1997) The taxonomy of three commercially important species of mitten crabs of the genus Eriocheir de Hann, 1835 (Crustacea: Decapod: Brachyura: Grapsidae). Raffles Bull Zool 45:445–476

    Google Scholar 

  • Hänfling B, Weetman D (2003) Characterization of microsatellite loci for the Chinese mitten crab, Eriocheir sinensis. Mol Ecol Notes 3:15–17

    Article  Google Scholar 

  • Ng NK, Guo JY, Ng PKL (1999) Generic affinities of Eriocheir leptognathus and E formosa with description of a new genus (Brachyura: Grapsidae: Varuninae). J Crustac Biol 19:154–170

    Article  Google Scholar 

  • Rousset F (2008) GENEPOP′007: a complete re-implementation of the GENEPOP software for Windows and Linux. Mol Ecol Resour 8(1):103–106

    Article  Google Scholar 

  • Sakai T (1935) Crustacea of Jehol. (Brachyura) (Order Decapoda). In: Report of the first scientific expedition to Manchoukuo, section V, division 1, part II, article, pp 1–16

  • Sakai T (1976) Crabs of Japan and adjacent seas. Kodansha, Tokyo

    Google Scholar 

  • Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, New York, pp 479–483

    Google Scholar 

  • Tang B, Zhou K, Song D, Yang G, Dai A (2003) Molecular systematics of the Asian mitten crabs, genus Eriocheir (Crustacea: Brachyura). Mol Phylogenet Evol 29:309–316

    Article  CAS  PubMed  Google Scholar 

  • Wang CH, Li CH, Li SF (2008) Population structure and demographic history of mitten crab (Eriocheir, sensu stricto) along the coast of mainland China inferred from mitochondrial loci. Mol Ecol 17:3515–3527

    PubMed  Google Scholar 

  • Weir BS, Cockerham CC (1984) Estimating F-statistics for the analysis of population structure. Evolution 38(6):1358–1370

    Article  Google Scholar 

  • Xu JW, Chan TY, Tsang LM, Chu KH (2009) Phylogeography of the mitten crab Eriocheir sensu stricto in East Asian: Pleistocene isolation, population expansion and secondary contact. Mol Phylogenet Evol 52:45–56

    Article  PubMed  Google Scholar 

  • Yamasaki I, Yoshizaki G, Yokota M, Strussmann CA, Watanabe S (2006) Mitochondrial DNA variation and population structure of the Japanese mitten crab Eriocheir japonica. Fish Sci 72:299–309

    Article  CAS  Google Scholar 

  • Zhu ZY, Shi YH, Le GW (2006) Isolation and characterization of polymorphic microsatellite from Chinese mitten crab, Eriocheir sinensis. Mol Ecol Notes 6:838–839

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors would like to thank Qinling Yang and Qin Chen for their kind assistance in performing the experiment. The authors give special thanks to Prof. Terry A. Dick (University of Manitoba, Canada) for his kind comments and polish on the manuscript. This work was supported by the Shanghai Science and Technology Project (grant no. 09391911100), the biological breeding project of National Development and Reform Commission (grant no. 2008–2223), and the Shanghai Leading Academic Discipline Project (grant no. Y1101).

Author information

Authors and Affiliations

  1. Key Laboratory of Aquatic Genetic Resources and Utilization, Ministry of Agriculture, Shanghai Ocean University, 201306, Shanghai, China

    Qixuan Cheng, Jun Wang, Jiawei Xu & Chenghui Wang

  2. College of Food Science and Technology, Shanghai Ocean University, 201306, Shanghai, China

    Chunhong Yuan

  3. Laboratory of Aquaculture Genetics and Genomics, Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, 041-8611, Japan

    Chunhong Yuan & Tai-Hung Lee

Authors
  1. Qixuan Cheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Chunhong Yuan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jun Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jiawei Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Tai-Hung Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Chenghui Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Chenghui Wang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cheng, Q., Yuan, C., Wang, J. et al. Development of 20 microsatellite loci in the Japanese mitten crab Eriocheir japonica and cross-amplification in the Chinese mitten crab Eriocheir sinensis . Conservation Genet Resour 2 (Suppl 1), 47–50 (2010). https://doi.org/10.1007/s12686-009-9158-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12686-009-9158-9

Keywords

Search

Navigation