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Characterisation of an Australian fowlpox virus carrying a near-full-length provirus of reticuloendotheliosis virus

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Abstract

Fowlpox virus (FWPV), which is the type member of the genus Avipoxvirus, subfamily Chordopoxvirinae, family Poxviridae, can lead to significant losses to the poultry industry. Although a large number of fowlpox virus genomes have been sequenced and characterised globally, there are no sequences available at the genomic level from Australian isolates. Here, we present the first complete genome sequence of a fowlpox virus vaccine strain (FWPV-S) containing an integrated near-full-length reticuloendotheliosis virus (REV) provirus. The genome of FWPV-S showed the highest sequence similarity to a fowlpox virus from the USA (97.74% identity). The FWPV-S genome contained 16 predicted unique genes, while a further two genes were fragmented compared to previously reported FWPV genome sequences. Subsequent phylogenetic analysis showed that FWPV-S was most closely related to other fowlpox viruses. This is the first reported genome sequence of FWPV from Australia.

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Data availability

The complete sequence of the FWPV-S genome was deposited in the GenBank database under the accession number MW142017.

References

  1. Bolte AL, Meurer J, Kaleta EF (1999) Avian host spectrum of avipoxviruses. Avian Pathol 28(5):415–432. https://doi.org/10.1080/03079459994434

    Article  CAS  PubMed  Google Scholar 

  2. van Riper C, Forrester DJ (2007) Avian Pox. In: Thomas NJ, Hunter DB, Atkinson CT (eds) Infectious diseases of wild birds. Wiley Blackwell Publishing, Oxford

    Google Scholar 

  3. Carulei O, Douglass N, Williamson A-L (2017) Comparative analysis of avian poxvirus genomes, including a novel poxvirus from lesser flamingos (Phoenicopterus minor), highlights the lack of conservation of the central region. BMC Genomics 18(1):947. https://doi.org/10.1186/s12864-017-4315-0

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Tripathy DN, Reed WM (2017) Pox. In Diseases of Poultry, D.E. Swayne (Ed.). https://doi.org/10.1002/9781119421481.ch10

  5. Joshi LR, Bauermann FV, Hain KS, Kutish GF, Armién AG, Lehman CP, Neiger R, Afonso CL, Tripathy DN, Diel DG (2019) Detection of Fowlpox virus carrying distinct genome segments of Reticuloendotheliosis virus. Virus Res 260:53–59. https://doi.org/10.1016/j.virusres.2018.10.017

    Article  CAS  PubMed  Google Scholar 

  6. Hertig C, Coupar BE, Gould AR, Boyle DB (1997) Field and vaccine strains of fowlpox virus carry integrated sequences from the avian retrovirus, reticuloendotheliosis virus. Virology 235(2):367–376. https://doi.org/10.1006/viro.1997.8691

    Article  CAS  PubMed  Google Scholar 

  7. Tripathy DN, Schnitzlein WM, Morris PJ, Janssen DL, Zuba JK, Massey G, Atkinson CT (2000) Characterization of poxviruses from forest birds in Hawaii. J Wildl Dis 36(2):225–230. https://doi.org/10.7589/0090-3558-36.2.225

    Article  CAS  PubMed  Google Scholar 

  8. Niemeyer C, Favero CM, Kolesnikovas CKM, Bhering RCC, Brandão P, Catão-Dias JL (2013) Two different avipoxviruses associated with pox disease in Magellanic penguins (Spheniscus magellanicus) along the Brazilian coast. Avian Pathol 42(6):546–551. https://doi.org/10.1080/03079457.2013.849794

    Article  PubMed  Google Scholar 

  9. Boyle DB, Pye AD, Coupar BEH (1997) Comparison of field and vaccine strains of Australian fowlpox viruses. Arch Virol 142(4):737–748. https://doi.org/10.1007/s007050050115

    Article  CAS  PubMed  Google Scholar 

  10. Boyle DB, Bulach DM, Amos-Ritchie R, Adams MM, Walker PJ, Weir R (2012) Genomic sequences of Australian bluetongue virus prototype serotypes reveal global relationships and possible routes of entry into Australia. J Virol 86(12):6724–6731. https://doi.org/10.1128/JVI.00182-12

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Sarker S, Das S, Lavers JL, Hutton I, Helbig K, Imbery J, Upton C, Raidal SR (2017) Genomic characterization of two novel pathogenic avipoxviruses isolated from pacific shearwaters (Ardenna spp.). BMC Genomics 18(1):298. https://doi.org/10.1186/s12864-017-3680-z

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Sarker S, Roberts HK, Tidd N, Ault S, Ladmore G, Peters A, Forwood JK, Helbig K, Raidal SR (2017) Molecular and microscopic characterization of a novel Eastern grey kangaroopox virus genome directly from a clinical sample. Sci Rep 7(1):16472. https://doi.org/10.1038/s41598-017-16775-7

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Sarker S, Batinovic S, Talukder S, Das S, Park F, Petrovski S, Forwood JK, Helbig KJ, Raidal SR (2020) Molecular characterisation of a novel pathogenic avipoxvirus from the Australian magpie (Gymnorhina tibicen). Virology 540:1–16. https://doi.org/10.1016/j.virol.2019.11.005

    Article  CAS  PubMed  Google Scholar 

  14. Sarker S, Athukorala A, Raidal SR (2021) Molecular characterisation of a novel pathogenic avipoxvirus from an Australian passerine bird, mudlark (Grallina cyanoleuca). Virology 554:66–74. https://doi.org/10.1016/j.virol.2020.12.011

    Article  CAS  PubMed  Google Scholar 

  15. Benson DA, Cavanaugh M, Clark K, Karsch-Mizrachi I, Lipman DJ, Ostell J, Sayers EW (2013) GenBank. Nucleic Acids Res. https://doi.org/10.1093/nar/gks1195

    Article  PubMed  PubMed Central  Google Scholar 

  16. Hillary W, Lin S-H, Upton C (2011) Base-By-Base version 2: single nucleotide-level analysis of whole viral genome alignments. Microbial Inform Exp 1:2–2. https://doi.org/10.1186/2042-5783-1-2

    Article  CAS  Google Scholar 

  17. Katoh K, Standley DM (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol 30(4):772–780. https://doi.org/10.1093/molbev/mst010

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Afonso CL, Tulman ER, Lu Z, Zsak L, Kutish GF, Rock DL (2000) The genome of fowlpox virus. J Virol 74(8):3815–3831. https://doi.org/10.1128/jvi.74.8.3815-3831.2000

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Offerman K, Carulei O, van der Walt AP, Douglass N, Williamson A-L (2014) The complete genome sequences of poxviruses isolated from a penguin and a pigeon in South Africa and comparison to other sequenced avipoxviruses. BMC Genomics 15(1):1–17. https://doi.org/10.1186/1471-2164-15-463

    Article  CAS  Google Scholar 

  20. Sarker S, Isberg SR, Milic NL, Lock P, Helbig KJ (2018) Molecular characterization of the first saltwater crocodilepox virus genome sequences from the world’s largest living member of the Crocodylia. Sci Rep 8(1):5623. https://doi.org/10.1038/s41598-018-23955-6

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

Dr. Sarker is the recipient of an Australian Research Council Discovery Early Career Researcher Award (Grant number DE200100367) funded by the Australian government. We also gratefully acknowledge the funding contributed by the Australian Biosecurity CRC for Emerging Infectious Disease in support of this work.

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Author notes

  1. Timothy R. Bowden

    Present address: Australian Centre for Disease Preparedness, CSIRO Australian Animal Health Laboratory, Geelong, VIC, 3220, Australia

Authors and Affiliations

  1. Department of Physiology, Anatomy and Microbiology, School of Life Sciences, La Trobe University, Melbourne, VIC, 3086, Australia

    Subir Sarker & Ajani Athukorala

  2. Australian Animal Health Laboratory, CSIRO Livestock Industries, Geelong, VIC, 3220, Australia

    Timothy R. Bowden & David B. Boyle

Authors
  1. Subir Sarker
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  2. Ajani Athukorala
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  3. Timothy R. Bowden
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  4. David B. Boyle
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Contributions

Conceptualization, SS, TRB, DBB; formal analysis, SS, AA, DBB; investigation, SS, TRB, DBB; methodology, SS, TRB, DBB; writing—original draft, SS; writing—review and editing, SS, AA, TRB, DBB. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Subir Sarker.

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Sarker, S., Athukorala, A., Bowden, T.R. et al. Characterisation of an Australian fowlpox virus carrying a near-full-length provirus of reticuloendotheliosis virus. Arch Virol 166, 1485–1488 (2021). https://doi.org/10.1007/s00705-021-05009-x

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  • DOI: https://doi.org/10.1007/s00705-021-05009-x

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