Abstract
The authors describe a fluorometric assay for the determination of Hg(II). A naphthalimide derivative is used as a label for a thymine (T) rich ssDNA, and graphene oxide magnetized with Fe3O4 nanoparticles acts as a quencher and preconcentrators. In the absence of Hg(II), the labeled ssDNA does not separate from the magnetized graphene oxide. As a result, fluorescence is fully quenched. In the presence of Hg(II), a T-Hg(II)-T link is formed dues to the highly affinity between T and Hg(II). Hence, fluorescence is restored. The assay has a linear response in the 1.0 to 10.0 nM Hg(II) concentration range, and a 0.65 nM detection limit. The method is selective and sensitive. It was applied to the analysis of spiked environmental water samples, and data agreed well with those obtained by atomic fluorescence spectrometry.
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Acknowledgements
This work was financially supported by the National Natural Science Foundation of China (21507034, 51521006), the Natural Science Foundation of Guangdong Province, China (2016A030310022), and the Pearl River S&T Nova Program of Guangzhou (201710010134).
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Li, MK., Hu, LY., Niu, CG. et al. A fluorescent DNA based probe for Hg(II) based on thymine-Hg(II)-thymine interaction and enrichment via magnetized graphene oxide. Microchim Acta 185, 207 (2018). https://doi.org/10.1007/s00604-018-2689-6
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DOI: https://doi.org/10.1007/s00604-018-2689-6