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Magnetic microparticle-based SELEX process for the identification of highly specific aptamers of heart marker--brain natriuretic peptide

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Abstract

The brain natriuretic peptide (BNP) is known to be an effective indicator of heart failure. It has been widely adopted as a parameter for the evaluation of heart function of cardiovascular and cerebrovascular diseases (CVDs). Current immune-recognition based methods for the detection of BNP are limited, to a certain extent, by the poor stability of the antibody and by high costs. The availability of an aptamer specific for BNP would greatly assist in the rapid and early diagnosis of CVDs. In order to screen for such an aptamer by the SELEX method, we have used magnetic microparticles (m-MPs) as the separation substrate for immobilization of target BNP. The use of m-MPs for rapid separation of combined aptamers enables bound oligonucleotides to be separated directly, quickly, and with high efficiency. After 14 rounds of selection, a panel of six aptamers against BNP was identified. Their dissociation constants range from 12.5 to 139 nM. The classical technique for conjugation of a target to m-MPs is known to be applicable to various fields, and we conclude that this m-MP-based SELEX process provides a general strategy for screening of specific aptamers against various analytes.

Rapid selection of aptamer to brain natriuretic peptide (BNP) with high affinity and specificity for rapid detection and daily monitoring of heart functions with the magnetic microparticles-based screening platform.

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Acknowledgements

This work is financially supported by the Huangshan Young Scholar Fund of Hefei University of Technology (407–037025), the NSFC grant of 31328009 and 31301460, the Science and Technology Research Project of General AQSIQ of China (201210127, 201310135), the 12th 5 Years Key Programs (2012BAK08B01-2, 2012BAK17B10, SS2012AA101001), National and Zhejiang Public Benefit Research Project (201313010, 2014C32051) and the Fundamental Research Funds for the Central Universities 2013HGCH0008 and partly supported by 62YYD201309 and 2012BAD43B03 .

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Authors and Affiliations

  1. Department of Materials Science and Chemical Engineering, Ningbo University, No 818#, Fenghua Road, Ningbo, 315211, People’s Republic of China

    Ying Wang & **xuan Cao

  2. School of Biotechnology and Food Engineering, Anhui Provincial Key Lab of Functional Materials & Devices, Hefei University of Technology, Hefei, 23009, People’s Republic of China

    Ying Wang, **g**g Wu, Feng Xue, Jun Teng & Wei Chen

  3. National Engineering Lab of Food Storage and Transportation, Nan**g University of Finances and Economics, Nan**g, 210023, People’s Republic of China

    Yinji Chen

  4. **njiang Academy of Agricultral And Reclamation Science, **njiang, China

    Chunxia Lu

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  1. Ying Wang
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  2. **g**g Wu
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  3. Yinji Chen
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  4. Feng Xue
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  5. Jun Teng
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  6. **xuan Cao
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  7. Chunxia Lu
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  8. Wei Chen
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Correspondence to Chunxia Lu or Wei Chen.

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Ying Wang and **g**g Wu contributed equally to this paper.

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Wang, Y., Wu, J., Chen, Y. et al. Magnetic microparticle-based SELEX process for the identification of highly specific aptamers of heart marker--brain natriuretic peptide. Microchim Acta 182, 331–339 (2015). https://doi.org/10.1007/s00604-014-1338-y

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