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Identification and map** of molecular markers linked to the tuberculate fruit gene in the cucumber (Cucumis sativus L.)

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Abstract

Warty fruit is one of the highly valuable external quality traits related to the market values of cucumber. Genetic analysis has shown that a single dominant gene, Tu (Tuberculate fruit), determines the warty fruit trait in the cucumber plant. An F2 population (247 individuals) from the cross of S06 × S52 was used for the map** of the Tu/tu locus. By combining bulked segregant analysis with the sequence-related amplified polymorphism (SRAP) and simple sequence repeat (SSR) markers, 15 markers (9 SRAPs and 6 SSRs) linked to the Tu/tu locus were identified. Of nine SRAP markers, three closely linked to the Tu/tu locus were successfully converted into sequence characterized amplified region (SCAR) markers. The Tu/tu locus was mapped between the co-dominant SSR marker SSR16203 and the SCAR marker C_SC933, at a genetic distance of 1.4 and 5.9 cM, respectively. Then the linked SSR markers in the study were used as anchor loci to locate the Tu/tu locus on cucumber chromosome 5. Moreover, the validity analysis of the C_SC69 and C_SC24 markers was performed with 62 cucumber lines of diverse origins, showing that the two SCAR markers can be used for marker-assisted selection (MAS) of the warty fruit trait in cucumber breeding. The information provided in this study will facilitate the map-based cloning of the Tu/tu gene.

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Acknowledgments

The authors wish to thank Prof. Sanwen Huang (Chinese Academy of Agricultural Sciences) for kindly providing SSR markers, Research Professor Lihuang Zhu (Chinese Academy of Sciences) for giving guidance and advice in paper writing and Dr. Rentao Song (Shanghai University) for his technical assistance in the BAC library. This work was supported by the National Natural Science Foundation of China (No. 30671111), the Shanghai Leading Academic Discipline Project (No. B209) and the International Cucumber Genome Initiative 2008-Z42 (2009-2).

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Authors and Affiliations

  1. School of Agriculture and Biology, Shanghai Jiaotong University, Dongchuan Road, Minhang District, 200240, Shanghai, China

    Weiwei Zhang, Huanle He, Yuan Guan, Hui Du, Lihua Yuan, Zheng Li, Danqing Yao, Junsong Pan & Run Cai

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  1. Weiwei Zhang
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  2. Huanle He
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  3. Yuan Guan
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  4. Hui Du
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  8. Junsong Pan
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Correspondence to Junsong Pan or Run Cai.

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Communicated by A. Kilian.

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Zhang, W., He, H., Guan, Y. et al. Identification and map** of molecular markers linked to the tuberculate fruit gene in the cucumber (Cucumis sativus L.). Theor Appl Genet 120, 645–654 (2010). https://doi.org/10.1007/s00122-009-1182-3

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  • DOI: https://doi.org/10.1007/s00122-009-1182-3

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