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Development and fine map** of three co-dominant SCAR markers linked to the M/m gene in the cucumber plant (Cucumis sativus L.)

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Abstract

Owing to its diverse sex types, the cucumber plant has been studied widely as a model for sex determination. In addition to environmental factors and plant hormones, three major genes—F/f, M/m, and A/a—regulate the sex types in the cucumber plant. By combining the bulked segregant analysis (BSA) and the sequence-related amplified polymorphism (SRAP) technology, we identified eight markers linking to the M/m locus. Among them, the two closely linked SRAP markers flanking the M/m locus were the co-dominant marker ME1EM26 and the dominant marker ME1EM23. Further, the co-dominant marker ME8SA7 co-segregated with the M/m locus. With the chromosome walking method using the cucumber genomic bacterial artificial chromosome (BAC) library, we successfully developed a co-dominant SCAR marker S_ME1EM23 from the ME1EM23 sequence. Along with the other two co-dominant SCAR markers S_ME1EM26 and S_ME8SA7 (developed from ME1EM26 and ME8SA7, respectively) in a larger segregating population (900 individuals), the M/m locus was mapped between S_ME1EM26 (5.4 cM) and S_ME1EM23 (0.7 cM), and S_ME8SA7 co-segregated with it.

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Acknowledgments

The authors wish to thank Dr. Rentao Song (Shanghai University) for his technical assistance in BAC library. This work was supported by National Natural Science Foundation of China (No. 30500289), National “863” Project (No. 2008AA10Z150), and Shanghai Leading Academic Discipline Project (No. B209).

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Authors and Affiliations

  1. Shanghai Jiaotong University, School of Agriculture and Biology, Shanghai, China

    Zheng Li, Junsong Pan, Yuan Guan, Qianyi Tao, Huanle He & Run Cai

  2. Department of Horticulture, Shenyang Agriculture University, Shenyang, China

    Longting Si

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  1. Zheng Li
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  2. Junsong Pan
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  3. Yuan Guan
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  4. Qianyi Tao
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  7. Run Cai
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Correspondence to Run Cai.

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Communicated by R. Hagemann.

Z. Li and J. Pan contribute equally to this article.

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Li, Z., Pan, J., Guan, Y. et al. Development and fine map** of three co-dominant SCAR markers linked to the M/m gene in the cucumber plant (Cucumis sativus L.). Theor Appl Genet 117, 1253–1260 (2008). https://doi.org/10.1007/s00122-008-0859-3

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