Abstract
Ascrobate free-radical reductase (EC 1.6.5.4) from potato tubers was purified to apparent homogencity by a method which included ammonium-sulfate precipitation, gel filtration and chromatography on diethylaminoethyl cellulose and hydroxylapatite. Gel filtration and gel electrophoresis showed that the purified enzyme was monomeric with a molecular weight of about 42 000. Enzyme activity was heat lable and severely inhibited by thiol reagents. The Km values for enzyme substrates were estimated.
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Abbreviations
- AFR:
-
ascorbate free radical
- AsA:
-
ascorbic acid
- DE-32(52):
-
diethylaminoethyl cellulose
- Tricine:
-
N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]-glycine
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Borraccino, G., Dipierro, S. & Arrigoni, O. Purification and properties of ascorbate free-radical reductase from potato tubers. Planta 167, 521–526 (1986). https://doi.org/10.1007/BF00391228
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DOI: https://doi.org/10.1007/BF00391228