Abstract
As shown in Chapter 1, plant hormones and other compounds which show physiological effects on plants are of widespread occurrence in the plant kingdom. The discovery and characterization of these compounds has depended upon isolating them in a pure state. Recent advances in purification techniques, especially the development of chromatography, enable the purification of even small amounts of components contained in plants. Furthermore the chromatographic techniques can now be applied as analytical tools for the detection or identification of plant components without isolation. In this chapter these various techniques including extraction, purification, and identification are discussed, centering on plant hormones, i.e., auxins, gibberellins, cytokinins, abscisic acid and related compounds, and ethylene. Some of these techniques have been reviewed recently (Hillman, 1978).
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Yokota, T., Murofushi, N., Takahashi, N. (1980). Extraction, Purification, and Identification. In: MacMillan, J. (eds) Hormonal Regulation of Development I. Encyclopedia of Plant Physiology, vol 9. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67704-5_3
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