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An improvement of auxin extraction procedure and its application to cultured plant cells

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Summary

The senescent cultured tobacco (Nicotiana tabacum L.) cells, XD6S, when extracted with customary procedure, contained much auxin. The use of new extraction procedure using dichloromethane which does not extract much indolepyruvic acid revealed that the cultured tobacco cells do not contain measurable auxin at any stage of culture.

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Abbreviations

IAA:

indoleacetic acid

IPyA:

indolepyruvic acid

Trp:

tryptophan

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Authors and Affiliations

  1. Department of Biology, College of General Education, University of Tokyo, Komaba, Meguro, 153, Tokyo, Japan

    S. Atsumi, S. Kuraishi & T. Hayashi

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  1. S. Atsumi
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  2. S. Kuraishi
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  3. T. Hayashi
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Atsumi, S., Kuraishi, S. & Hayashi, T. An improvement of auxin extraction procedure and its application to cultured plant cells. Planta 129, 245–247 (1976). https://doi.org/10.1007/BF00398265

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  • DOI: https://doi.org/10.1007/BF00398265

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