Abstract
Much of the attention and interest in modern molecular biology is focussed on the regulation of gene expression. Factors influencing or mediating such regulation are often best studied using gene fusions. Gene fusions can be defined as DNA constructions (performed in vitro or in vivo) that result in the coding sequences from one gene (reporter) being transcribed and/or translated under the direction of the controlling sequences of another gene (controller). Most frequently, the reporter encodes an enzyme. Use of reporter enzymes can greatly enhance the sensitivity of gene fusion measurements, and can also facilitate many types of analysis.
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References
Helmer, G., Casadaban, M., Bevan, M.W., Kayes, L. and Chilton M.-D. (1984) Bio/technology 2, 520–527.
Depicker, A., Stachel, S., Dhaese, P1, Zambryski, P. and Goodman H. (1982) J. Mol. Applied Genetics 1, 561–575.
Bevan, M.P., Barnes, W. and Chilton, M.-D. (1983) Nucl. Acids Res. 11: 369–385.
De Greve, H., Dhaese, P., Seurinck, J., Lemmers, M., van Montagu, M. and Schell J. (1982) J. Mol. Genetics 1, 499–513.
Otten, L. and Schilperoot,R.A. (1978) Biocjem. Biophys. Res. Commun. 527, 497–500.
Jones, J.D.G., Dunsmuir, P. and Bedbrook J. (1985) EMBO J. 4, 2411–2418.
Herrera-Estrella, L., Depicker A., Van Montagu, M. and Schell, J. (1983) Nature 303, 209–213.
Herrera-Estrella L., De Block, MN., Messens, E., Hernalsteens, J.-P., van Montagu, M. and Schell J. (1983) EMBO J. 2, 987–995.
Bevan, M.W., Flavell, R.B. and Chilton M.-D. (1983) Nature 304, 184–187.
Fraley, R.T., Rogers, S.G., Horsch, R.B., Sanders, P.S., Flick, J.S., Adams, S.P., Bittner, M.L., Brand, L.A., Fink, C.L., Fry, J.S., Galluppi, G.R. Goldberg, S.B., Hoffmann, N.L. and Woo, S.C. (1983) Proc. Nat. Acad. Sci. U.S.A. 80, 4803–4807.
Van den Broeck, G., Timko, M., Kausch, A.P., Cashmore, A.R., van Montagu, M. and Herrera-Estrella, L. (1985) Nature 313, 358–363.
Gorman, C.M., Moffatt, L.F., and Howard, B.H. (1982) Mol. Cell. Biol. 2, 1044–1051.
Reiss, B., Sprengel, R., Will, H. and Schaller, H. (1984) Gene 30, 217–223.
Ow, D.W., Wood, K.V., DeLuca, M., DeWet, J.R., Helinski, D.R. and Howell, S.H. (1986) Science, 234, 856–859.
DeLuca, M. and McElroy, W.D. (1978) Methods Enzymol. 57, 3–15.
Jefferson, R.A. (1985) PhD Dissertation, University of Colorado, Boulder.
Jefferson, R.A., Burgess, S.M. and Hirsh, D. (1986) Proc. Nat. Acad. Sci. U.S.A. 83, 8447–8451.
Jefferson, R.A., Klass, M., Wolf, N. and Hirsh, D. (1987) J. Mol. Biol. 193, 41–46.
Jefferson, R.A., Kavanagh, T.A. and Bevan, M.W. (1987) EMBO J. 6, 3901–3907.
Jefferson, R.A., Kavanagh, T.A. and Bevan, M.W. (1987) Biochem. Soc. Trans. 15; 17–18.
Jefferson, R.A., Harkins, K.R., Bevan, M.W., Kavanagh, T.A. and Galbraith, D.W. (1987) unpublished data.
Stoeber, F. (1961) These de Docteur es Sciences, Paris.
Novel, G., and Novel, M. (1973) Mol. Gen. Genet. 120, 319–335.
Bevan, M.W. (1984) Nucl. Acids Res. 12, 8711–8721.
Odell, J.T., Nagy, F. and Chua, N.-H. (1985) Nature 313, 810–812.
Mazur, B.J. and Chui, C-F. (1985) Nucl. Acids Res. 13, 2373–2386.
Bevan, M., Barker, R., Goldsbrough, A., Jarvis, M. and Iturriagga, G. (1986) Nucl. Acids Res. 14, 4625–4631.
Horsh, R.B., Fraley, R.T., Rogers, S.G., Sanders, P.R., Lloyd, A. and Hoffmann, N. (1984) Science, 223, 496–498.
Sheerman, S. and Bevan, M. (1988) Plant Cell Reports 7: 13–16.
Guilbault, G. (1973) Practical Fluorescence: Theory, Methods and Techniques, Dekker, New York.
O’Brien, T.P. and McCully, M.E. (1981). The Study of Plant Structure: Principles and Selected Methods. Termarcarphi Pty. Ltd., Melbourne, Australia.
Lojda, Z. (1970) Histochemie 23, 266–288.
Pearse, A.G.E. (1972) Histochemistry: Theoretical and Applied Third Edition, Vol II, pp. 808–840. Churchill Livingstone, Edinburgh, Scotland.
Kozak, M. (1983) Microbiol. Rev. 47, 1–45.
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Jefferson, R.A. (1988). Plant Reporter Genes: The Gus Gene Fusion System. In: Setlow, J.K. (eds) Genetic Engineering. Genetic Engineering, vol 10. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-7081-3_13
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DOI: https://doi.org/10.1007/978-1-4615-7081-3_13
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