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Measurement of Luciferase Rhythms

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Plant Circadian Networks

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1158))

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Abstract

Firefly luciferase (LUC) is a sensitive and versatile reporter for the analysis of gene expression. Transgenic plants carrying CLOCK GENE promoter:LUC fusions can be assayed with high temporal resolution. LUC measurement is sensitive, noninvasive, and nondestructive and can be readily automated, greatly facilitating genetic studies. For these reasons, LUC fusion analysis is a mainstay in the study of plant circadian clocks.

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Acknowledgements

This work was supported by grants from the National Science Foundation (IOS-0923752 and IOS-1025965) to C.R.M.

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Authors and Affiliations

  1. Department of Biological Sciences, Dartmouth College, Hanover, NH, 03755, USA

    C. Robertson McClung & Qiguang **e

Authors
  1. C. Robertson McClung
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  2. Qiguang **e
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Corresponding author

Correspondence to C. Robertson McClung .

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Editors and Affiliations

  1. Molecular Cell Physiology, University of Bielefeld, Germany

    Dorothee Staiger

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McClung, C.R., **e, Q. (2014). Measurement of Luciferase Rhythms. In: Staiger, D. (eds) Plant Circadian Networks. Methods in Molecular Biology, vol 1158. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0700-7_1

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  • DOI: https://doi.org/10.1007/978-1-4939-0700-7_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0699-4

  • Online ISBN: 978-1-4939-0700-7

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