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Holotomography: Refractive Index as an Intrinsic Imaging Contrast for 3-D Label-Free Live Cell Imaging

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Advanced Imaging and Bio Techniques for Convergence Science

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1310))

Abstract

Live cell imaging provides essential information in the investigation of cell biology and related pathophysiology. Refractive index (RI) can serve as intrinsic optical imaging contrast for 3-D label-free and quantitative live cell imaging, and provide invaluable information to understand various dynamics of cells and tissues for the study of numerous fields. Recently significant advances have been made in imaging methods and analysis approaches utilizing RI, which are now being transferred to biological and medical research fields, providing novel approaches to investigate the pathophysiology of cells. To provide insight into how RI can be used as an imaging contrast for imaging of biological specimens, here we provide the basic principle of RI-based imaging techniques and summarize recent progress on applications, ranging from microbiology, hematology, infectious diseases, hematology, and histopathology.

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Authors and Affiliations

  1. Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, South Korea

    Doyeon Kim

  2. Department of Physics, KAIST, Daejeon, South Korea

    Sangyun Lee, Moosung Lee, Juntaek Oh & YongKeun Park

  3. Department of Biological Sciences, KAIST, Daejeon, South Korea

    Su-A Yang

  4. KAIST Institute Health Science and Technology, Daejeon, South Korea

    YongKeun Park

  5. Tomocube Inc., Daejeon, South Korea

    YongKeun Park

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  1. Doyeon Kim
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  2. Sangyun Lee
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  3. Moosung Lee
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  4. Juntaek Oh
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  5. Su-A Yang
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  6. YongKeun Park
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Correspondence to YongKeun Park .

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  1. Department of Convergence Medicine, University of Ulsan College of Medicine, Asan Institute for Life Sciences, Asan Medical Center, Seoul, Korea (Republic of)

    Jun Ki Kim

  2. Department of Radiology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea (Republic of)

    Jeong Kon Kim

  3. Department of Convergence Medicine, University of Ulsan College of Medicine, Asan Institute for Life Sciences, Asan Medical Center, Seoul, Korea (Republic of)

    Chan-Gi Pack

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Kim, D., Lee, S., Lee, M., Oh, J., Yang, SA., Park, Y. (2021). Holotomography: Refractive Index as an Intrinsic Imaging Contrast for 3-D Label-Free Live Cell Imaging. In: Kim, J.K., Kim, J.K., Pack, CG. (eds) Advanced Imaging and Bio Techniques for Convergence Science. Advances in Experimental Medicine and Biology, vol 1310. Springer, Singapore. https://doi.org/10.1007/978-981-33-6064-8_10

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