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Label-Free Multimodal Multiphoton Intravital Imaging

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Optical Imaging in Human Disease and Biological Research

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 3233))

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Abstract

Label-free intravital optical imaging is an emergent visualization tool that is not only useful for basic biological research, but also for preclinical research with potential translational clinical applications. The complete absence of exogenous labeling or genetic alterations avoids plausible harmful perturbation to biological processes and the pristine physiological environment, as the endogenous biomolecules enable intrinsic imaging contrasts to interrogate various live multicellular organisms of interest. This tool has evolved from single-modality, single-photon imaging into multimodal multiphoton imaging, in order to gain different contrasts simultaneously during imaging sessions, and permit long-term time-lapse studies that have begun to spawn more diverse applications.

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Acknowledgements

We thank all of our colleagues for their dedicated efforts to advance the field of IVM and its applications in biology and medicine. While this chapter has focused on the more narrowly defined topic of label-free IVM, we acknowledge that a more comprehensive review and inclusion of more results was not practical due to length limitations. This review was prepared with support in part from the National Institutes of Health (R01CA213149, R01EB023232, R01CA241618). Additional information can be found at: http://biophotonics.illinois.edu.

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Authors and Affiliations

  1. Biophotonics Imaging Laboratory, University of Illinois at Urbana-Champaign, Urbana-Champaign, IL, USA

    Jaena Park, Haohua Tu, Marina Marjanovic & Stephen A. Boppart

  2. Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana-Champaign, IL, USA

    Jaena Park, Haohua Tu, Marina Marjanovic & Stephen A. Boppart

  3. Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana-Champaign, IL, USA

    Jaena Park, Marina Marjanovic & Stephen A. Boppart

  4. Carle Illinois College of Medicine, University of Illinois at Urbana-Champaign, Urbana-Champaign, IL, USA

    Marina Marjanovic & Stephen A. Boppart

  5. Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana-Champaign, IL, USA

    Stephen A. Boppart

  6. Cancer Center at Illinois, University of Illinois at Urbana-Champaign, Urbana-Champaign, IL, USA

    Stephen A. Boppart

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  1. Jaena Park
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  2. Haohua Tu
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  3. Marina Marjanovic
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  4. Stephen A. Boppart
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Corresponding author

Correspondence to Stephen A. Boppart .

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Editors and Affiliations

  1. State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China

    Xunbin Wei

  2. School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China

    Bobo Gu

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Park, J., Tu, H., Marjanovic, M., Boppart, S.A. (2021). Label-Free Multimodal Multiphoton Intravital Imaging. In: Wei, X., Gu, B. (eds) Optical Imaging in Human Disease and Biological Research. Advances in Experimental Medicine and Biology, vol 3233. Springer, Singapore. https://doi.org/10.1007/978-981-15-7627-0_7

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