Laboratory Techniques for Studying Biliary Contrast Media

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Radiocontrast Agents

Part of the book series: Handbook of Experimental Pharmacology ((HEP,volume 73))

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Abstract

Little training is provided for researchers in the proper care and handling of laboratory animals, selecting the best species for use in research projects, selecting the proper experimental technique to obtain the data which will provide them with the information needed to answer the questions being asked, or preparing for the technical problems which need to be avoided or anticipated and handled so that the desired project will not be a waste of time, resources, and energy. Sometimes a poor experiment carried out carefully and meticulously will be of some scientific value despite shortcomings in the original experimental design. However, a well-conceived study, regardless of its intellectual clarity and brilliance, will be of little or no value when pursued without the necessary laboratory background or experience, without the proper techniques, and without proper awareness of the possible technical difficulties. Assumptions drawn from data which are obtained using techniques not clearly understood, obtained using improper techniques, and obtained from ignorance of potential difficulties of techniques can lead the researcher to reach mistaken conclusions about the questions being asked.

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References

  1. Hafez ESE (1970) Reproduction and breeding techniques for laboratory animals. Lea and Febiger, Philadelphia

    Google Scholar 

  2. Mitruka BM, Rawnsley HM, Vadehra DV (1976) Animals for medical research. Models for the study of human disease. Wiley, New York

    Google Scholar 

  3. Universities Federation for Animal Welfare (1976) The UFAW handbook on the care and management of laboratory animals, 5th edn. Churchill Livingstone, New York

    Google Scholar 

  4. Federation of American Societies for Experimental Biology (1969) Comparative anesthesia in laboratory animals. Miller EV, Ben M, Cass JS (eds) Fed Proc 28:1373–1586

    Google Scholar 

  5. Mitruka BM, Rawnsley HM (1977) Clinical biochemical and hematological reference values in normal experimental animals. Masson, New York

    Google Scholar 

  6. Kaplan HM, Timmons EH (1979) The rabbit. A model for the principles of mammalian physiology and surgery. Academic, New York

    Google Scholar 

  7. Cornelius CE (1976) Rates of choleresis in various species. Digest Dis 21:426–428

    Article  CAS  Google Scholar 

  8. Goodman LS, Gilman A (1975) The pharmacological basis of therapeutics, 5th edn. Macmillan, New York

    Google Scholar 

  9. Gillespie NA (1943) The signs of anesthesia. Curr Res Anesth Analg 22:275–282

    Google Scholar 

  10. Raventos J (1954) The distribution in the body and metabolic fate of barbiturates. J Pharm Pharmacol 6:217–235

    Article  PubMed  CAS  Google Scholar 

  11. Chiasson RB (1975) Laboratory anatomy of the white rat, 3rd edn. Brown, Dubuque

    Google Scholar 

  12. Vanlerenberghe J (1965) The effects of hypothermia on biliary function. In: Taylor W (ed) The biliary system, a symposium of the NATO Advanced Study Institute. Blackwell, Oxford, pp 263–276

    Google Scholar 

  13. Roberts RJ, Klaassen CD, Plaa GL (1967) Maximum biliary excretion of bilirubin and sulfobromophthalein during anesthesia-induced alteration of rectal temperature. Proc Soc Exp Biol Med 125:313–316

    PubMed  CAS  Google Scholar 

  14. Wheeler HO, Ramos OL (1960) Determinants of the flow and composition of bile in the unanesthetized dog during constant infusions of sodium taurocholate. J Clin Invest 39:161–170

    Article  PubMed  CAS  Google Scholar 

  15. Wheeler HO, Meltzer JI, Bradley SE (1960) Biliary transport and hepatic storage of sulfobromophthalein sodium in the unanesthetized dog, in normal man, and in patients with hepatic disease. J Clin Invest 39:1131–1144

    Article  PubMed  CAS  Google Scholar 

  16. Berk RN, Goldgerger LE, Loeb PM (1974) The role of bile salts in the hepatic excretion of iopanoic acid. Invest Radiol 9:7–15

    Article  PubMed  CAS  Google Scholar 

  17. Berk RN, Loeb PM, Cobo-Frenkel A, Barnhart JL (1976) Saturation kinetics of choleretic effects of iodoxamate and iodipamide. Radiology 119:529–535

    PubMed  CAS  Google Scholar 

  18. Berk RN, Loeb PM, Cobo-Frenkel A, Barnhart JL (1977) The biliary and urinary excretion of sodium tyropanoate and sodium ipodate in dogs. Invest Radiol 12:85–95

    Article  PubMed  CAS  Google Scholar 

  19. Loeb PM, Barnhart JL, Berk RN (1978) The dependence of the biliary excretion of iopanoic acid on bile salts. Gastroenterology 74:174–181

    PubMed  CAS  Google Scholar 

  20. Nelson JA, Staubus AE, Riegelman S (1975) Saturation kinetics of iopanoate in the dog. Invest Radiol 10:371–377

    Article  PubMed  CAS  Google Scholar 

  21. Thomas HE (1941) An improved cannula for gastric and intestinal fistulas. Proc Soc Exp Biol Med 46:260–265

    Google Scholar 

  22. Gronwall R, Cornelius CE (1970) Maximum biliary excretion of sulfobromophthalein sodium in sheep. Am J Dig Dis 15:37–48

    Article  PubMed  CAS  Google Scholar 

  23. Alpert S, Mosher M, Shanske A, Arias IM (1969) Multiplicity of hepatic excretory mechanisms for organic anions. J Gen Physiol 53:238–247

    Article  PubMed  CAS  Google Scholar 

  24. Gronwall RR, Engelking LR, Anwer MS, Erickson DF, Klentz RD (1975) Bile secretion in ponies with biliary fistulas. Am J Vet Res 36:653–654

    PubMed  CAS  Google Scholar 

  25. Engelking LR, Gronwall R (1979) Effects of fasting on hepatic bile acid clearance. Proc Soc Exp Biol Med 161:123–127

    PubMed  CAS  Google Scholar 

  26. Miller LL, Bly CG, Watson ML, Bale WF (1951) The dominant role of the liver in plasma synthesis. A direct study of the isolated perfused rat liver with the aid of lysine-ε-14C. J Exp Med 94:431–453

    Article  PubMed  CAS  Google Scholar 

  27. Hems R, Ross BD, Berry MN, Krebs HA (1966) Gluconeogenesis in the perfused rat liver. Biochem J 101:284–292

    PubMed  CAS  Google Scholar 

  28. Berry MN, Friend DS (1969) High-yield preparation of isolated rat liver parenchymal cells. J Cell Biol 43:506–520

    Article  PubMed  CAS  Google Scholar 

  29. Hatoff DE, Hardison WGM (1979) Induced synthesis of alkaline phosphatase by bile acids in rat liver cell culture. Gastroenterology 77:1062–1067

    PubMed  CAS  Google Scholar 

  30. Strange RC, Chapman BT, Johnson JD, Nimmo IA, Percy-Robb IW (1979) Partitioning of bile acids into subcellular organelles and the in vivo distribution of bile acids in rat liver. Biochim Biophys Acta 573:535–545

    PubMed  CAS  Google Scholar 

  31. Willard HH, Merritt LL Jr, Dean JA (1965) Instrumental methods of analysis, 4th edn. D Van Nostrand, Princeton

    Google Scholar 

  32. Woldseth R (1973) All you ever wanted to know about x-ray energy spectrometry. Kevex, Burlingame

    Google Scholar 

  33. Johnson EL, Stevenson R (1978) Basic liquid chromatography. Varian, Ralp Alto

    Google Scholar 

  34. Malloy HT, Evelyn KA (1937) The determination of bilirubin with the photoelectric colorimeter. J Biol Chem 119:481–484

    CAS  Google Scholar 

  35. Zak B, Boyle AJ (1952) A simple method for the determination of organic bound iodine. J Am Pharmacol Assoc 41:260–262

    Article  CAS  Google Scholar 

  36. Turley SD, Dietschy JM (1978) Re-evaluation of the 3α-hydroxysteroid dehydrogenase assay for total bile acids in bile. J Lipid Res 19:924–928

    PubMed  CAS  Google Scholar 

  37. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275

    PubMed  CAS  Google Scholar 

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© 1984 Springer-Verlag Berlin Heidelberg

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Barnhart, J.L. (1984). Laboratory Techniques for Studying Biliary Contrast Media. In: Sovak, M. (eds) Radiocontrast Agents. Handbook of Experimental Pharmacology, vol 73. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-69515-5_9

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  • DOI: https://doi.org/10.1007/978-3-642-69515-5_9

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-69517-9

  • Online ISBN: 978-3-642-69515-5

  • eBook Packages: Springer Book Archive

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