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Applicability of ISAP and RAPD Techniques for Capsicum Collection Genoty**

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Advanced Crop Improvement, Volume 2

Abstract

Bulgaria was the secondary gene pool for many crops, and one of the first was pepper. However, during the political transforming and economic crises, the lands for growing pepper (Capsicum spp.) were reduced, and thereafter, the genetic diversity was lost. With pepper, Bulgaria still has priority providing on European scale valuable pepper germplasm, and this priority should be evaluated and preserved. We present our efforts to characterize pepper accessions using RAPD as well as the retroelement-based Inter-SINE Amplified Polymorphism (ISAP) method initially developed for potatoes. Several short interspersed nuclear element (SINE) families were active within the common ancestor of potato and pepper. We studied the degree of polymorphisms in a collection of 73 pepper genotypes, divided into six groups, using ISAP with primers derived from seven Solanaceae SINE families as well as two subfamilies. Two primer pairs from the families SolS-II and SolS-V generated the most fragments and most informative banding patterns. These SINE-based ISAP reactions are best suited for identifying species of the Capsicum genus. The most polymorphic profiles within all studied were generated by C. baccatum accessions. In contrast, intraspecific application of the SINE-based markers yielded a high percentage of conserved ISAP fragments. From a total of 56 C. annuum accessions, only three of them with two different profiles were identified. Our results demonstrate that potato-based SolS-SINE primers can be adapted for molecular genoty** in peppers. The low intraspecies polymorphism generated by ISAP forced us to investigate RAPD as an alternative low-cost genoty** approach. RAPD was successfully applied on a group of mutant lines and corresponding source lines, carrying valuable breeding traits. Despite the low polymorphic levels, we have identified four RAPD primers, capable to discriminate among several genotypes.

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Abbreviations

BSA:

Bovin serum albumin

CTAB:

Hexadecyltrimethylammonium bromide [(C16H33)N(CH3)3]Br

EDTA:

Ethylenediaminetetraacetic acid [CH2N(CH2CO2H)2]2

M:

Multiplex reaction

Sol:

Solanaceae

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Acknowledgments

The authors acknowledge financial support from the International Atomic Energy Agency, grant numbers BUL/5/016 and RER/5/024. We are grateful for the contribution of Prof. Thomas Schmidt (Dresden University of Technology, Germany) in the development and introduction of the ISAP technique as a means of identifying and studying members of the family Solanaceae.

Funding

 This research was funded by International Atomic Energy Agency, Technical Cooperation projects BUL/5/016 and RER/5/024.

Author information

Authors and Affiliations

  1. Molecular Biology Laboratory, Maritsa Vegetable Crops Research Institute, Agricultural Academy, Plovdiv, Bulgaria

    Nasya Tomlekova

  2. Agricultural Academy, Tobacco and Tobacco Products Institute, Markovo, Bulgaria

    Velichka Spasova-Apostolova

  3. Department of Horticulture, Agricultural University, Plovdiv, Bulgaria

    Nikolay Panayotov

  4. Department of Biochemistry, Sofia University “St. Kliment Ohridski”, Sofia, Bulgaria

    Ivelin Panchev

  5. General Directorate of Agricultural Research and Policies, Ministry of Agriculture and Forestry Universiteler Mah, Ankara, Cankaya, Turkey

    Fatma Sarsu

  6. Department of Botany, Abdul Ahad Azad Memorial Degree College Bemina, Cluster University, Srinagar, Jammu and Kashmir, India

    Mohammad Rafiq Wani

  7. Institute of Botany, Technische Universität Dresden, Dresden, Germany

    Kathrin M. Seibt

Authors
  1. Nasya Tomlekova
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  2. Velichka Spasova-Apostolova
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  3. Nikolay Panayotov
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  4. Ivelin Panchev
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  5. Fatma Sarsu
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  6. Mohammad Rafiq Wani
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  7. Kathrin M. Seibt
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Editor information

Editors and Affiliations

  1. Mutation Breeding Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, Uttar Pradesh, India

    Aamir Raina

  2. Department of Botany, Abdul Ahad Azad Memorial Degree College Bemina, Cluster University, Srinagar, Jammu and Kashmir, India

    Mohammad Rafiq Wani

  3. Department of Botany, Pandit Deendayal Upadhyaya Adarsha Mahavidyalaya (PDUAM), Eraligool, Karimganj, Assam, India

    Rafiul Amin Laskar

  4. Molecular Biology Laboratory, Maritsa Vegetable Crops Research Institute, Agricultural Academy, Plovdiv, Bulgaria

    Nasya Tomlekova

  5. Mutation Breeding Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, Uttar Pradesh, India

    Samiullah Khan

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© 2023 The Author(s), under exclusive license to Springer Nature Switzerland AG

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Tomlekova, N. et al. (2023). Applicability of ISAP and RAPD Techniques for Capsicum Collection Genoty**. In: Raina, A., Wani, M.R., Laskar, R.A., Tomlekova, N., Khan, S. (eds) Advanced Crop Improvement, Volume 2. Springer, Cham. https://doi.org/10.1007/978-3-031-26669-0_3

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