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Determination of Tetracyclines and Their 4-Epimers in Milk by Solid-Phase Extraction (SPE) and Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

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Chemical Food Contaminants Analysis

Part of the book series: Methods and Protocols in Food Science ((MPFS))

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Abstract

Tetracyclines are antimicrobial agents commonly employed in veterinary medicine. These substances have the potential to leave residues in food products, such as milk, that may cause adverse effects in humans who consume food containing these chemical residues. The quantitative determination of tetracycline residues in milk can be accomplished through the analytical method outlined here, which utilizes solid phase extraction (SPE) for sample preparation and LC-MS/MS for the analysis of the resulting extract.

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Authors and Affiliations

  1. Instituto Nacional de Controle de Qualidade em Saúde (INCQS)/Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, RJ, Brazil

    Rosana Gomes Ferreira, Mararlene Ulberg Pereira, Amanda da Silva Rio & Bernardete Ferraz Spisso

Authors
  1. Rosana Gomes Ferreira
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  2. Mararlene Ulberg Pereira
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  3. Amanda da Silva Rio
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  4. Bernardete Ferraz Spisso
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Correspondence to Bernardete Ferraz Spisso .

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Editors and Affiliations

  1. Ministry of Agriculture, Livestock, and, Sao Jose, Brazil

    Rodrigo Hoff

  2. Ministry of Agriculture Livestock and Su, Federal Distsrict, Brazil

    Luciano Molognoni

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Ferreira, R.G., Pereira, M.U., da Silva Rio, A., Spisso, B.F. (2024). Determination of Tetracyclines and Their 4-Epimers in Milk by Solid-Phase Extraction (SPE) and Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS). In: Hoff, R., Molognoni, L. (eds) Chemical Food Contaminants Analysis. Methods and Protocols in Food Science . Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3806-4_11

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  • DOI: https://doi.org/10.1007/978-1-0716-3806-4_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3805-7

  • Online ISBN: 978-1-0716-3806-4

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