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Hydrophilic Interaction Liquid Chromatography (HILIC) Enrichment of Glycopeptides Using PolyHYDROXYETHYL A

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Recombinant Glycoproteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2762))

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Abstract

Glycosylation of proteins is an important post-translational modification that plays a role in a wide range of biological processes, including immune response, intercellular signaling, inflammation, and host-pathogen interaction. Abnormal protein glycosylation has been correlated with various diseases. However, the study of protein glycosylation remains challenging due to its low abundance, microheterogeneity of glycosylation sites, and low ionization efficiency. During the past decade, several methods for enrichment and for isolation of glycopeptides from biological samples have been developed and successfully employed in glycoproteomics research. In this chapter, we discuss the sample preparation protocol and the strategies for effectively isolating and enriching glycopeptides from biological samples, using PolyHYDROXYETHYL A as a hydrophilic interaction liquid chromatography (HILIC) enrichment technique.

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Authors and Affiliations

  1. Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, USA

    Mona Goli, Peilin Jiang, Mojibola Fowowe, Md Abdul Hakim & Yehia Mechref

Authors
  1. Mona Goli
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  2. Peilin Jiang
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  3. Mojibola Fowowe
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  4. Md Abdul Hakim
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  5. Yehia Mechref
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Corresponding author

Correspondence to Yehia Mechref .

Editor information

Editors and Affiliations

  1. Center for Global Health, University of New Mexico Health Sciences Center, Albuquerque, NM, USA

    Steven B. Bradfute

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Goli, M., Jiang, P., Fowowe, M., Hakim, M.A., Mechref, Y. (2024). Hydrophilic Interaction Liquid Chromatography (HILIC) Enrichment of Glycopeptides Using PolyHYDROXYETHYL A. In: Bradfute, S.B. (eds) Recombinant Glycoproteins. Methods in Molecular Biology, vol 2762. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3666-4_16

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  • DOI: https://doi.org/10.1007/978-1-0716-3666-4_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3665-7

  • Online ISBN: 978-1-0716-3666-4

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