Abstract
Probiotics have a number of positive impacts, including regulating immunological responses, activating the immune system, and preserving homeostasis and the balance of gut microbes. Probiotics have many positive benefits for animal and human health, which has led to the development of a wide variety of probiotic agents. Probiotics have been shown to have antiviral properties, which include modulating immunological, microbial, chemical, and physical barriers through a variety of probiotics, probiotic metabolites, and host signaling pathways. This chapter details the methods that can be used to identify the antiviral activity of postbiotics against viruses. These include toxicity of the test postbiotic compounds assessed by MTT, maintenance of cell lines, determination of the infectivity of the virus, and antiviral assays. These protocols are used for the evaluation of postbiotics that can be carried out by a single individual in Category 2 + or 3 containment laboratories.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Cunningham-Rundles S, Ahrné S, Bengmark S, Johann-Liang R, Marshall F, Metakis L, Califano C, Dunn AM, Grassey C, Hinds G, Cervia J (2000) Probiotics and immune response. Am J Gastroenterol 95(1):S22–S25
Murcia P, Donachie W, Palmarini M (2009) Viral pathogens of domestic animals and their impact on biology, medicine and agriculture. In: Encyclopedia of microbiology. Academic Press, p 805. https://doi.org/10.1016/B978-012373944-5.00368-0
VanderWaal K, Deen J (2018) Global trends in infectious diseases of swine. Proc Natl Acad Sci 115(45):11495–11500
Knežević A, Stepanović S, Ćupić M, Jevtović D, Ranin J, Jovanović T (2005) Reduced quantity and hydrogen-peroxide production of vaginal lactobacilli in HIV positive women. Biomed Pharmacother 59(9):521–523
Liu Z, Xu C, Tian R, Wang W, Ma J, Gu L, Liu F, Jiang Z, Hou J (2021) Screening beneficial bacteriostatic lactic acid bacteria in the intestine and studies of bacteriostatic substances. J Zhejiang Univ Sci B 22(7):533–547
Tachedjian G, Aldunate M, Bradshaw CS, Cone RA (2017) The role of lactic acid production by probiotic Lactobacillus species in vaginal health. Res Microbiol 168:782–792. https://doi.org/10.1016/j.resmic.2017.04.001
Botić T, Danø T, Weingartl H, Cencič A (2007) A novel eukaryotic cell culture model to study antiviral activity of potential probiotic bacteria. Int J Food Microbiol 115(2):227–234
Lange-Starke A, Petereit A, Truyen U, Braun PG, Fehlhaber K, Albert T (2014) Antiviral potential of selected starter cultures, bacteriocins and D, L-lactic acid. Food Environ Virol 6:42–47
Welch JL, **ang J, Okeoma CM, Schlievert PM, Stapleton JT (2020) Glycerol monolaurate, an analogue to a factor secreted by Lactobacillus, is virucidal against enveloped viruses, including HIV-1. Mbio 11(3):e00686–e00620
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
About this chapter
Cite this chapter
Sounderrajan, V., Rao, S.S., T., T., Parthasarathy, K. (2024). Antiviral Activity of Postbiotics. In: Dharumadurai, D. (eds) Postbiotics. Methods and Protocols in Food Science . Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3421-9_28
Download citation
DOI: https://doi.org/10.1007/978-1-0716-3421-9_28
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3420-2
Online ISBN: 978-1-0716-3421-9
eBook Packages: Springer Protocols