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Electrophysiological Techniques for Studying Tactile Perception in Rats

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Somatosensory Research Methods

Part of the book series: Neuromethods ((NM,volume 196))

Abstract

Neurons communicate by electrical and chemical signals. Neurophysiological studies measuring and manipulating these signals are therefore of utmost importance to understand neural function. In this chapter, we review an extensive set of tools used in our laboratory to study tactile processing in rats. After a very brief summary of the anatomy and physiology of the sense of touch, instrumentation for generating mechanical stimuli is covered in detail. Next, techniques for studying mechanoreceptive afferents are presented. Remaining sections include electroencephalography (EEG), electrocorticography (EcoG), local field potentials (LFP), and extracellular spike recordings for the brain. Acute and chronic preparations for recording from the somatosensory cortex are reviewed in line with the current state-of-the-art technology for electrodes and equipment. Dedicated sections are devoted to electrical microstimulation of neural tissue and microinjection of drugs, which allow manipulation of the somatosensory system for basic and applied research. The material presented in this chapter is also useful for guiding neural engineering applications such as neuroprostheses and brain-machine interfaces (BMI), at their initial developmental stages.

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Acknowledgments

This work was supported by TÜBİTAK Grant 117F481 within the European Union’s FLAG-ERA JTC 2017 project GRAFIN.

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Authors and Affiliations

  1. Department of Neurobiology, School of Medicine, Duke University, Durham, NC, USA

    Sevgi Öztürk

  2. Department of Biomedical Engineering, Çorlu Faculty of Engineering, Tekirdağ Namık Kemal University, Tekirdağ, Turkey

    İsmail Devecioğlu

  3. Department of Electrical and Electronics Engineering, Faculty of Engineering and Natural Sciences, İstanbul Bilgi University, İstanbul, Turkey

    Bige Vardar

  4. Catalan Institute of Nanoscience and Nanotechnology—ICN2, Barcelona, Spain

    Fikret Taygun Duvan

  5. Institute of Biomedical Engineering, Boğaziçi University, İstanbul, Turkey

    Burak Güçlü

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  3. Bige Vardar
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  4. Fikret Taygun Duvan
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  5. Burak Güçlü
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Corresponding author

Correspondence to Burak Güçlü .

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  1. School of Sport, Exercise and Rehabilitation Sciences, University of Birmingham, Birmingham, UK

    Nicholas Paul Holmes

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Öztürk, S., Devecioğlu, İ., Vardar, B., Duvan, F.T., Güçlü, B. (2023). Electrophysiological Techniques for Studying Tactile Perception in Rats. In: Holmes, N.P. (eds) Somatosensory Research Methods. Neuromethods, vol 196. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3068-6_16

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  • DOI: https://doi.org/10.1007/978-1-0716-3068-6_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3067-9

  • Online ISBN: 978-1-0716-3068-6

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