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Screening of Endophytes for Biocatalytic Tools

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Endophytic Microbes: Isolation, Identification, and Bioactive Potentials

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Abstract

Endophytic communities thrive in environments that are rich in biologically active compounds. To survive, endophytes have adapted to produce secondary metabolites mimicking their host plant and synthesize the required enzymes which are used for cell wall penetration and colonization of the host plant. Therefore, endophytic microorganisms could be a source of novel enzymes able to catalyze the organic transformation of a wide range of valuable fine chemicals derived from natural products.

This chapter presents various strategies to discover new biocatalyst within the endophytic community. The chemical entities naturally present in the plant suggest that the associated endophytes could be able to synthesize, metabolize, or modify a portion of the chemical structure. Enrichment and isolation in the presence of the metabolite of interest is a useful strategy to selectively isolate endophytes with the metabolic capacity to modify or degrade the compound. In addition, the use of different growing media, including one that mimics the internal environment of the plant, has also proven advantageous.

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Author information

Authors and Affiliations

  1. Laboratorio de Biocatálisis y Biotransformaciones, DEPBIO-DQO, Universidad de la República (UdelaR), Montevideo, Uruguay

    David Gonzalez, Emiliana Botto, Sonia Rodríguez Giordano & Paula Rodríguez Bonnecarrere

  2. Laboratorio de Microbiología, DEPBIO Facultad de Química, Universidad de la República (UdelaR), Montevideo, Uruguay

    Sonia Rodríguez Giordano

Authors
  1. David Gonzalez
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  2. Emiliana Botto
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  3. Sonia Rodríguez Giordano
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  4. Paula Rodríguez Bonnecarrere
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Corresponding author

Correspondence to Paula Rodríguez Bonnecarrere .

Editor information

Editors and Affiliations

  1. Department of Life Sciences, Sri Sathya Sai University for Human ExcellenceNavanihal, Kamalapura, Kalaburagi, Karnataka, India

    A. Sankaranarayanan

  2. C.G. Bhakta Institute of Biotechnology, Uka Tarsadia University, Surat, Gujarat, India

    Natarajan Amaresan

  3. C.G. Bhakta Institute of Biotechnology, Uka Tarsadia University, Surat, Gujarat, India

    Mitesh Kumar Dwivedi

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Gonzalez, D., Botto, E., Giordano, S.R., Rodríguez Bonnecarrere, P. (2023). Screening of Endophytes for Biocatalytic Tools. In: Sankaranarayanan, A., Amaresan, N., Dwivedi, M.K. (eds) Endophytic Microbes: Isolation, Identification, and Bioactive Potentials. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2827-0_21

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  • DOI: https://doi.org/10.1007/978-1-0716-2827-0_21

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2826-3

  • Online ISBN: 978-1-0716-2827-0

  • eBook Packages: Springer Protocols

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