Determination of Volatile Metabolites in Vinegar by Solid-Phase Microextraction–Gas Chromatography–Mass Spectrometry (SPME–GC–MS)

  • Protocol
  • First Online:
Mass Spectrometry for Metabolomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2571))

Abstract

Solid-phase microextraction (SPME) is an easy, sensitive, and environmentally friendly technique that has been employed, coupled to gas chromatography or liquid chromatography, to determine a huge amount of analytes with different volatilities. The present work describes the procedure to follow in order to determine volatile compounds in vinegar by SPME–GC–MS.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free ship** worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free ship** worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  1. Solieri L, Giudici P (2009) Vinegars of the world. In: Solieri L, Giudici P (eds) Vinegars of the world. Springer, Milan

    Chapter  Google Scholar 

  2. Natera R, Castro R, García-Moreno MV, Hernández MJ, García-Barroso CG (2003) Chemometric studies of vinegars from different raw materials and processes of production. J Agric Food Chem 51:3345–3351

    Article  CAS  Google Scholar 

  3. Šikuten I, Štambuk P, Kontić JK, Maletić E, Tomaz I, Preiner D (2021) Optimization of SPME-arrow-GC/MS method for determination of free and bound volatile organic compounds from grape skins. Molecules 26:7409

    Article  Google Scholar 

  4. Castro R, Natera R, Durán E, García-Barroso C (2008) Application of solid phase extraction techniques to analyse volatile compounds in wines and other enological products. Eur Food Res Technol 228:1–18

    Article  CAS  Google Scholar 

  5. Ferrer-Valverde MA, Sánchez-Palomo E, Osorio-Alises M, Chaya C, González-Viñas MA (2021) Volatile and sensory characterization of La Mancha Trujillo melons over three consecutive harvests. Foods 10:1683

    Article  Google Scholar 

  6. Arthur CL, Pawliszyn J (1990) Solid phase microextraction with thermal desorption using fused silica optical fibers. Anal Chem 62:2145–2148

    Article  CAS  Google Scholar 

  7. Louch D, Motlagh S, Pawliszyn J (1992) Dynamics of organic compound extraction from water using liquid-coated fused silica fibers. Anal Chem 64:1187–1199

    Article  CAS  Google Scholar 

  8. De la Calle D, Reichenbacher M, Danzer KJ, Hurlbeck C, Bartzsch C, Feller K (1998) Analysis of wine bouquet components using headspace solid-phase microextraction-capillary gas chromatography. J High Resolut Chromatogr 21:373–377

    Article  Google Scholar 

  9. Yang X, Peppard T (1994) Solid-phase microextraction for flavor analysis. J Agric Food Chem 42:1925–1930

    Article  CAS  Google Scholar 

  10. Jia M, Zhang QH, Min DB (1998) Optimization of solid-phase microextraction analysis for headspace flavor compounds of orange juice. J Agric Food Chem 46:2744–2747

    Article  CAS  Google Scholar 

  11. Resende dos Santos R, Orlando RM, De Lourdes Z, Menezes HC (2021) Assessment of polycyclic aromatic hydrocarbons and derivatives in beer using a new cold fiber-solid phase microextraction system. Food Control 126:108104

    Article  CAS  Google Scholar 

  12. Ji X (2021) Comparative investigation of volatile components and bioactive compounds in beers by multivariate analysis. Flavour Fragr J 36:374–383

    Article  CAS  Google Scholar 

  13. Zhou C, Zhou Y, Hu Y, Li B, Zhang R, Zheng K, Liu J, Wang J, Zuo M, Liu S (2021) Integrated analysis of metabolome and volatile profiles of germinated brown rice from the Japonica and Indica Subspecies. Foods 10:2448

    Article  CAS  Google Scholar 

  14. Natera R, Castro R, García-Moreno MV, García-Rowe F, García-Barroso C (2002) Headspace solid-phase microextraction analysis of aroma compounds in vinegar: validation study. J Chromatogr A 967:261–267

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Remedios Castro .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Castro, R., Durán-Guerrero, E. (2023). Determination of Volatile Metabolites in Vinegar by Solid-Phase Microextraction–Gas Chromatography–Mass Spectrometry (SPME–GC–MS). In: González-Domínguez, R. (eds) Mass Spectrometry for Metabolomics. Methods in Molecular Biology, vol 2571. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2699-3_2

Download citation

  • DOI: https://doi.org/10.1007/978-1-0716-2699-3_2

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2698-6

  • Online ISBN: 978-1-0716-2699-3

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Navigation