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Protocol for 6 Hz Corneal Stimulation in Rodents for Refractory Seizures

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Experimental and Translational Methods to Screen Drugs Effective Against Seizures and Epilepsy

Part of the book series: Neuromethods ((NM,volume 167))

Abstract

The 6-hertz (6 Hz) model is an effective tool to screen drugs for psychomotor seizures and/or treatment-resistant focal seizures. It is known to identify the drugs or molecules acting via different mechanisms, unlike the traditional models. Seizure is induced by corneal stimulation (6 Hz, 0.2 ms for 3 s duration) using electroconvulsometer. The characteristic features seen in rodents are stun position and minimal clonic phase, followed by stereotyped behavior observed for 120 s, at stimulus of different intensities like 22, 32, or 44 mA. The animals are considered as protected if no stereotype behavior is observed and the animal resumes normal exploratory behavior within 10 s. Despite being effective in identifying the antiseizure potential of certain drugs possessing newer mechanisms, the model cannot correlate the neuronal and pathophysiological adaptations occurring in chronic cases of epilepsy, which is considered as its drawback.

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Author information

Authors and Affiliations

  1. Department of Biomedical Sciences, Gulf Medical University, Ajman, UAE

    Razia Khanam

  2. Neurobehavioral Pharmacology Laboratory, Department of Pharmacology, School of Pharmaceutical Education and Research (SPER), Jamia Hamdard, New Delhi, India

    Divya Vohora

Authors
  1. Razia Khanam
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  2. Divya Vohora
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Corresponding authors

Correspondence to Razia Khanam or Divya Vohora .

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Editors and Affiliations

  1. Neurobehavioral Pharmacology Laboratory, Department of Pharmacology, School of Pharmaceutical Education and Research (SPER), Jamia Hamdard, New Delhi, India

    Divya Vohora

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Khanam, R., Vohora, D. (2021). Protocol for 6 Hz Corneal Stimulation in Rodents for Refractory Seizures. In: Vohora, D. (eds) Experimental and Translational Methods to Screen Drugs Effective Against Seizures and Epilepsy. Neuromethods, vol 167. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1254-5_9

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  • DOI: https://doi.org/10.1007/978-1-0716-1254-5_9

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1253-8

  • Online ISBN: 978-1-0716-1254-5

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