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Bioreactors in Stem Cell Biology

Methods and Protocols

  • Book
  • © 2022
  • Latest edition

Overview

Editors:
  1. Kursad Turksen

    1. Ottawa, Canada

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2436)

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About this book

This new edition brings together some of the latest developments and protocols reflecting the rapidity with which bioreactor technologies are advancing and being applied. Given that the use of bioreactors in cell biology is becoming more commonplace as attempts are made to scale-up production of various types of cells for regenerative medicine and pharmaceutical purposes, this volume provides practical guidance for navigating research projects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Bioreactors in Stem Cell Biology: Methods and Protocols, Second Edition will benefit both established investigators and newcomers to this dynamic area of study.

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Benchtop Bioreactors in Mammalian Cell Culture: Overview and Guidelines

    • Angélique Schmid, Emanuel Kreidl, Martin Bertschinger, Patrick Vetsch
    Pages 1-15

  3. Volumetric Mass Transfer Coefficient Measurement in a Stirred Tank Reactor

    • Aysegul Inam, Ezgi Rojda Taymaz, Mehmet Emin Uslu, Baris Binay, Irem Deniz
    Pages 17-25

  4. Fabrication Protocol for Thermoplastic Microfluidic Devices: Nanoliter Volume Bioreactors for Cell Culturing

    • Elif Gencturk, Senol Mutlu, Kutlu O. Ulgen
    Pages 27-38

  5. Expansion of Human Pluripotent Stem Cells in Stirred Tank Bioreactors

    • Marites T. Woon, Puspa R. Pandey, Inbar Friedrich Ben-Nun
    Pages 39-53

  6. High-Efficiency Differentiation of Human Pluripotent Stem Cells to Hematopoietic Stem/Progenitor Cells in Random Positioning Machine Bioreactors

    • **aohua Lei, Chiyuan Ma, Yu**g Cao, Yue **ong, Jian V. Zhang, Enkui Duan
    Pages 55-66

  7. Integrating Human-Induced Pluripotent Stem Cell Expansion Capability and Cardiomyocyte Differentiation Potential in a Microcarrier Suspension Culture

    • Valerie Ho, Gerine Tong, Alan Lam, Shaul Reuveny, Steve Oh
    Pages 67-81

  8. Chemically Defined, Xeno-Free Expansion of Human Mesenchymal Stem Cells (hMSCs) on Benchtop-Scale Using a Stirred Single-Use Bioreactor

    • Misha Teale, Valentin Jossen, Dieter Eibl, Regine Eibl
    Pages 83-111

  9. Large-Scale Expansion of Umbilical Cord Mesenchymal Stem Cells with Microcarrier Tablets in Bioreactor

    • Huanye Xu, Zhongxiao Cong, Yuanyuan Zhang, Wei Liu, **aojun Yan, Yanan Du
    Pages 113-125

  10. Optimized Method to Improve Cell Activity in 3D Scaffolds Under a Dual Real-Time Dynamic Bioreactor System

    • Flavia Pedrini, Moema A. Hausen, Eliana A. R. Duek
    Pages 127-134

  11. In Vitro 3D Mechanical Stimulation to Tendon-Derived Stem Cells by Bioreactor

    • Ziming Chen, Peilin Chen, Rui Ruan, Minghao Zheng
    Pages 135-144

  12. Microcarrier-Supported Culture of Chondrocytes in Continuously Rocked Disposable Bioreactor

    • Kamil Wierzchowski, Maciej Pilarek
    Pages 145-156

  13. Tracheal In Vitro Reconstruction Using a Decellularized Bio-Scaffold in Combination with a Rotating Bioreactor

    • Georgia Pennarossa, Matteo Ghiringhelli, Fulvio Gandolfi, Tiziana A. L. Brevini
    Pages 157-165

  14. Bioreactor-Based De-epithelialization of Long-Segment Tracheal Grafts

    • Alba E. Marin-Araujo, Siba Haykal, Golnaz Karoubi
    Pages 167-182

  15. Production of Extracellular Vesicles Using a CELLine Adherent Bioreactor Flask

    • Anastasiia Artuyants, Vanessa Chang, Gabrielle Reshef, Cherie Blenkiron, Lawrence W. Chamley, Euphemia Leung et al.
    Pages 183-192

  16. Extracellular Vesicle Collection from Human Stem Cells Grown in Suspension Bioreactors

    • Xuegang Yuan, **ngchi Chen, Changchun Zeng, David G. Meckes Jr, Yan Li
    Pages 193-204

  17. Bacterial Nanocellulose-Based Grafts for Cell Colonization Studies: An In Vitro Bioreactor Perfusion Model

    • Max Wacker, Jan Riedel, Priya Veluswamy, Maximilian Scherner, Jens Wippermann, Heike Walles et al.
    Pages 205-222

  18. A Guideline to Set Up Cascaded Continuous Cultivation with E. coli Bl21 (DE3)

    • Julian Kopp, Oliver Spadiut
    Pages 223-240

  19. Applying Stirred Perfusion to 3D Tissue Equivalents to Mimic the Dynamic In Vivo Microenvironment

    • Henry W. Hoyle, Claire L. Mobbs, Stefan A. Przyborski
    Pages 241-256

  20. Bioreactor-Based Adherent Cells Harvesting from Microcarriers with 3D Printed Inertial Microfluidics

    • Lin Ding, Reza Moloudi, Majid Ebrahimi Warkiani
    Pages 257-266
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Editors and Affiliations

  • Ottawa, Canada

    Kursad Turksen

Bibliographic Information

  • Book Title: Bioreactors in Stem Cell Biology

  • Book Subtitle: Methods and Protocols

  • Editors: Kursad Turksen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2018-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2017-5Published: 21 January 2022

  • Softcover ISBN: 978-1-0716-2020-5Published: 22 January 2023

  • eBook ISBN: 978-1-0716-2018-2Published: 20 January 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 273

  • Number of Illustrations: 21 b/w illustrations, 53 illustrations in colour

  • Topics: Biotechnology, Stem Cells, Regenerative Medicine/Tissue Engineering

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