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Genic SSR Development and Diversity Assessment of Persian Halophytic Grass, Aeluropus littoralis

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Abstract

Aeluropus littoralis is a valuable halophyte grass belonging to the same family of wheat and is used as forage. Although A. littoralis has the potential to become an important genetic resource for improving salt and drought tolerance in economically important crops, no SSR markers have been developed for it. The main goal was to rapidly develop a set of genic SSR markers for A. littoralis. Repeat analysis of non-redundant EST sequences of Aeluropus and transferability assessment of 110 SSR-rich loci from rice and wheat were used to identify EST-SSRs. Then selected EST-SSR loci and some physiological traits includings Na+, K+ and Ash content were utilized for marker characterization and assessment of genetic diversity among A. littoralis accessions collected from all around the country. The results showed that 6.7% of EST records of A. littoralis comprising SSR motifs which was used for desiging 18 primer pairs (ALES). In addition 48 SSR loci (GDES) from 110 of the gramineae were shown to be transferable to A. littoralis based on the PCR profiles. Finally, genotypic clustering based on EST-SSR markers divided the accessions into seven groups. The accessions were also categorized into six groups according to the physiological traits. Our finding indicated that there are remarkable variations about 33% in coding regions of Iranian Aeluropus accessions. The results of both genotypic and physiologic clustering were partially consistent and most groups corresponded to geographic regions.

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Funding

This work was funded by the Genetics and Agricultural Biotechnology, Institute of Tabarestan (GABIT).

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Authors and Affiliations

  1. Department of Plant Breeding and Biotechnology, Faculty of Crop Science, Sari Agricultural Sciences and Natural Resources University (SANìRU), Sari, Iran

    Maryam Meidansary

  2. Center of Excellence in Electrochemistry, Faculty of Chemistry, University of Tehran, Tehran, Iran

    Najmeh Nasiri

  3. Department of Nanotechnology, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education, and Extension Organization (AREEO), Karaj, Iran

    Ehsan Shokri

  4. Department of Biotechnology, Faculty of New Technologies and Energy Engineering, Shahid Beheshti University (G.C), Tehran, Iran

    Hossein Askari

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  1. Maryam Meidansary
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Correspondence to Ehsan Shokri.

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Meidansary, M., Nasiri, N., Shokri, E. et al. Genic SSR Development and Diversity Assessment of Persian Halophytic Grass, Aeluropus littoralis. Cytol. Genet. 57, 320–334 (2023). https://doi.org/10.3103/S0095452723040096

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