Abstract
Cell cultures of Saussurea medusa produce valuable secondary metabolites, and jaceosidin and hispidulin are the major bioactive compounds. In the present study, the cultures were challenged by methyl jasmonate (MJ). The highest jaceosidin and hispidulin concentrations (65.2±3.67 mg/L and 12.3±0.47 mg/L) were achieved with 5 μM MJ added to 9-d-old subcultures, being 2.2-fold and 4.2-fold, respectively, higher than those from controls. The elicitor had little influence on cell growth, indicating that the changed biological processes did not include alterations in cell division. Furthermore, we observed that the activities of phenylalanine ammonia lyase were transiently increased after treatment with MJ, which suggests that this elicitor modifies jaceosidin and hispidulin production by regulating the phenylpropanoid pathway.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Fan, C. Q. and Yue, J. M. (2003), Bioorg. Med. Chem. 11, 703–708.
Szabo, E., Thelen, A., and Petersen, M. (1999), Plant Cell Rep. 18, 485–489.
Beerheide, W., Bernard, H. U., Tan, Y. J., Ganesan, A., Rice, W. G., and Ting, A. E. (1999), J. Natl. Cancer Inst. 91, 1211–1220.
Liu, L., **ao, X., and Zhang, L. (1985), J. Lanzhou Univ. Nat. Sci. 21, 80–83.
Zhao, D. X., Qiao, C. L., Wang, Y. (1998), Act. Bot. Sin 40, 515–520.
Zhao, D. X., Fu, C. X., Han, Y. S., and Lu, D. P. (2005), Process Biochem. 40, 739–745.
Pozo, M. J., Van, Loon, L. C., and Pieterse, C. M. J. (2005), J. Plant Growth Regul. 23, 211–220.
Turner, J. G., Ellis, C., and Devoto, A. (2002), Plant Cell 14 (suppl.), s153–164.
Schenk, P. M., Kazan, K. I., Wilson, Anderson, J. P., Richmond, T., Somerville, S. C., and Manners, J. M. (2000), Proc. Natl. Acad. Sci. USA 97, 11,655–11,660.
Kang, S. M., Jung, H. Y., Kang, Y. M., Yun, D. J., Bahk, J. D., Yang, J. K., and Choi, M. S. (2004), Plant Sci. 166, 745–751.
Sharan, M., Taguchi, G., Gonda, K., Jouke, T., Shimosaka, M., Hayashida, N., and Okazaki, M. (1998), Plant Sci. 132, 13–19.
Creelman, R. A. and Mullet, J. E. (1995), Proc. Natl. Acad. Sci. USA 92, 4114–4119.
Hahlbrock, K. and Scheel, D. (1989), Annu. Rev. Plant Physiol. Plant Mol. Biol. 40, 347–369.
Murashige, T. and Skoog, F. (1962), Physiol. Plant 15, 473–497.
Kuzovkina, I. N., Guseva, A. V., Alterman, I. E., and Karnachuk, R. A. (2001), Russ. J. Plant Physiol. 48, 448–452.
Hiroaki, H., Pengyu, H., and Kenichiro, I. (2003), Plant Cell Physiol. 44, 404–411.
Tamari, G., Bochorov, A., and Weiss, D. (1995), Physiol. Plant 94, 45–50.
Ketchum, R. E., Gibson, D. M., Croteau, R. B., and Shuler, M. L. (1999), Biotechnol. Bioeng. 62, 97–105.
Komaraiah, P., Amrutha, R. N., Kishor, P. B. K., and Ramakrishna, S. V. (2002), Enzyme Microb. Technol. 31, 634–639.
Mirjalili, N. and Linden, J. C. (1996), Biotechnol. Prog. 12, 110–118.
Ciddi, V., Srinvasan, V., and Shuler, M. L. (1995), Biotechnol. Lett. 17, 1343–1346.
Moreno, P. R. H., Poulsen, C., Heijden, R., and Verpoorte, R. (1996), Enzyme Microbiol. Technol. 18, 99–107.
Lu, M., Wong, H., and Teng, W. (2001), Plant Cell Rep. 20, 674–677.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Fu, CX., Cheng, LQ., Lv, XF. et al. Methyl jasmonate stimulates jaceosidin and hispidulin production in cell cultures of Saussurea medusa . Appl Biochem Biotechnol 134, 89–96 (2006). https://doi.org/10.1385/ABAB:134:1:89
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1385/ABAB:134:1:89