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Modulation of Picroside-I Biosynthesis in Grown Elicited Shoots of Picrorhiza kurroa In Vitro

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Abstract

Elicitors are considered as biostimulants for growth improvement and enhancement of secondary metabolite content. To date, only seaweed extract (SWE) powder has been studied for its effect on picroside-I (P-I) production in in vitro grown Picrorhiza kurroa plants. However, little is known at the molecular level about P-I production in P. kurroa plants upon SWE treatment. Here, we investigated the relative effects of supplying different elicitors including methyl jasmonate (MeJa), sodium nitroprusside (SNP), and abscisic acid (ABA) with SWE on plant growth and P-I production in addition to their effects at the molecular level reflecting the metabolic status of P-I biosynthesis. Our results indicated that only SWE, ABA, and SNP stimulated P-I production by 2.60-, 2.01-, and 1.35-fold, respectively, whereas MeJa decreased P-I content. Interestingly, SWE modulated all four integrating secondary metabolic pathways, covering almost all critical steps in the methylerythritol phosphate (MEP), mevalonate (MVA), iridoid, and phenylpropanoid pathways to stimulate P-I biosynthesis. SNP targeted the MVA/MEP pathways in conjunction with the iridoid pathway, whereas ABA modulated the phenylpropanoid pathway to increase the P-I content in P. kurroa. This is apparently the first report on treatment of different elicitors in in vitro grown P. kurroa plants for eliciting P-I content and exploring the role of different elicitors at the molecular level.

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Acknowledgments

The authors are thankful to the Jaypee University of Information Technology for providing the necessary research facilities and Department of Biotechnology, Ministry of Science and Technology, Govt. of India for providing research grant in the form of a programme support on high value medicinal plants to RSC and HS. We are also thankful to the Himalayan Forest Research Institute (HFRI), Manali, India for providing plant material of P. kurroa. The authors also gratefully acknowledge Sea6 Energy Pvt Ltd. for providing the seaweed extract powder.

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  1. Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Waknaghat, Solan, Himachal Pradesh, 173234, India

    Neha Sharma, Varun Kumar, Ra**der Singh Chauhan & Hemant Sood

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  1. Neha Sharma
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  2. Varun Kumar
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  3. Ra**der Singh Chauhan
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Correspondence to Hemant Sood.

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Sharma, N., Kumar, V., Chauhan, R.S. et al. Modulation of Picroside-I Biosynthesis in Grown Elicited Shoots of Picrorhiza kurroa In Vitro. J Plant Growth Regul 35, 965–973 (2016). https://doi.org/10.1007/s00344-016-9594-1

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