Abstract
Exploring innovative methods to provide essential nutrients and reducing ruminant greenhouse gas emission is crucial for animal production and diminishing global warming. This study was conducted to examine the efficacy of Moringa oleifera leaves (ML) in ruminants at 0%, 5%, 10%, 15%, 20%, 30% and 40% level in different roughage (R) and concentrate (C) (80R:20C, 70R:30C and 60R:40C) under in vitro conditions. Chemical composition of ML, concentrate mixture and berseem were estimated. Rumen fermentation parameters of male goat kids viz., total gas production, CH4, true dry matter digestibility (TDMD), organic matter digestibility (TOMD), partial fraction (PF), microbial biomass (MBP), ammonia (N), acetate, propionate, butyrate and acetate propionate ratio were observed under in vitro conditions. Results revealed that crude protein, organic matter and ethyl ether content were higher in ML as compared to concentrate mixture and berseem. Magnesium and iron content were also higher in ML as compared to concentrate and berseem. Total gas production, digestibility of DM and OM, MBP, acetate and propionate level were improved (P < 0.05) upto 10–20% replacement. In contrast, decreased in CH4 (%) and CH4 (mL/100 mg dDM) was noted with increased levels of ML incorporation. There was no change observed in ammonia, acetate: propionate ratios at all the three planes of nutrition. In this study, it is concluded that mixing Moringa oleifera leaves in feed can be used as protein supplement and reduce the methane emission without causing any effect on digestibility and rumen fermentation parameters. However, ML can be suggested for widespread practice to attain the sustainable animal production (10–20%) and to alleviate the global warming.
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Introduction
Livestock rearing is important for global food production. Animal production in farm is typically reduced due to the low quality and scarcity of animal feeds in tropical countries. Feedstuffs especially protein sources such as legumes, cereals and grains essential for animal development, have become very expensive and limited in many regions of the world (Choudhary et al. 2022). Hence, it is required for searching a substitute source of feed which are edible, rich in protein and minerals, low-priced and fulfils the basic needs of small ruminants.
The ruminant cattle industry significantly contributes to greenhouse gas (GHG) emission that cause global warming (Eisen and Brown 2022). Methane is one of the main GHG and its potency is twenty-five times as that of CO2. Ruminants are one major causes of emission of methane (81–92 MT) produced per year worldwide which is equal to total anthropogenic methane (23–27%). Methane is produced by enteric fermentation process in ruminants and contributes about 13% of methane emission from livestock in India (Gupta et al. 2018). Cattle contribute 49.10% enteric methane emission followed by buffalo, goat, sheep and others as 42.80%, 5.38%, 2.59% and 0.73% within agriculture. Different sources such as amino acids, organic acids, essential oils and exogenous enzymes have been used to alleviate the ruminant methane emission (Benetal et al. 2022; Kholif et al. 2022). Numerous studies have reported a reduction in enteric methane emission by feeding of tree leaves to ruminants and many workers have advocated their use as an alternative protein source for livestock (Ku-Vera et al. 2020).
Moringa oleifera is a perennial tree feed and also called as “miracle tree”. It is a multipurpose and fast-growing tree with nutritional and therapeutic properties that can be planted in a variety of climates including drought and heat, and can be harvested numerous times (Abbas et al. 2018). Its leaves contain sufficient quantity of minerals, proteins and vitamins according to the nutritional demand in lactating goats (Afzal et al. 2022). It is also having antioxidant properties such phenols, vitamin C, carotene and flavonoids (Saleem et al. 2020). It is an inexpensive protein constituent as compared to soyabean and sesame feed meals used in livestock feeding. Moringa oleifera leaves (ML) meal contains 9 times extra protein as compared to yogurt having good feeding effect and can be used as protein substitute in animal feed (Su and Chen 2020). The normal crude protein content in ML was 180–270 g CP/kg DM as reported by Kholif et al. (2018). Application of Moringa foliage improved the feed consumption, metabolic profile and growth performance of goat kids (Wankhede et al. 2022). ML are natural feed supplement which produce secondary metabolites like tannins and saponins, modify the pathways of rumen fermentation and prevent the growth of methanogens effectively (Zeru et al. 2022). ML strengthened the immune system and reduced oxidative stress in goats due to the presence of bioactive compounds (Al-Juhaimi et al. 2020; Teclegeorgish et al. 2021). Dong et al. (2019) reported that supplementation of ML in goats food improved content of fat milk and decrease the M. ruminantium which involved in methane production. Application of Moringa oil (4%) along with 30–50% of roughage ration decreased the methanogens and protozoa population but increase the Provotella which involved in rumen acidosis (Ebeid et al. 2009).
Gas production and rumen fermentation parameters
Animal feed composition having critical aspect to control the methane emission. Recently ruminant methane reduction approaches involved the addition of some inhibitors such as chemical, biological and natural animal feed to inhibit the growth of methanogenic microbes in gut of animals. ML are effective methanogen inhibitors and thus considered alternatives for rumen fermentation pathways. In this study increase in total gas production was observed upto 10% level. Gas production is mainly due to liberation of acetate, propionate and butyrate by the fermentation of carbohydrates. In the present study it is revealed that as the roughage contents increased methane production also augmented, but addition of ML reduces the methane production at 0, 5, 10, 15, 20, 30 and 40% levels. This might be due to the presence of ɑ-linolenic, tannins and saponins in ML (Machmüller et al. 2000). Presence of tannins and phenolics had antimicrobial effects which can be a main cause for methane reduction (Goel and Makkar 2012). Reduction in methane (17%) was also observed in ML treated ruminants over soyabean meal (Soliva et al. 2015). Supplementation of ML by replacing soyabean meal significantly reduces methane production, ammonia-N in steers and goats, but increased the production of CO2 reported by Elghandour et al. (2017a, b). ML feed decreased enteric methane emission and increased milk production in dairy cows as reported by Bashar et al. (2020). ML feeding may reduce the energy loss including methane and urinary nitrogen without having an effect on beef cattle production (Sultana et al. 2021).
Higher ruminal digestibility of fibers and other constituents in ML reportedly contributes to its considerably high energy concentration. Dey et al. (2014) also reported increased in the TDMD and TOMD contents on supplementation of M. oleifera leaves to wheat straw. Supplementation of M. oleifera improve digestibility, sustained outstanding situation and confirm better feeding value (Cohen-Zinder et al. 2016). Therefore, improvement in TDMD and TDMO in the present study might be due to higher degradability of Moringa leaves as both the parameters improved with incremental levels of concentrate replacement with ML. Li et al. (2019) reported that ML diet can enhance nutrient intake, nutritional digestibility and rumen fermentation in dairy Holsteins cows. Aregheore (2020) reported that ML supplementation (20%) in growing goats improved digestibility and weight gain.
The increasing level of ML in the experimental did not affect ammonical nitrogen concentration in any of the TMR. Ammonia-N ratio of ruminal in this study reached from 12.02 to 13.14 (mg/dl). This could be due to the total dietary nitrogen level was at par (iso-nitrogenous) or with a small difference, and thus nitrogen degradation in the rumen occurred in a similar fashion among the R:C ratio or within the same ratio in different level of ML replacement. Elghandour et al. (2017a, b) reported that ML supplementation decreased the ruminal ammonia-N and protozoal population. Application of ML decreased ruminal ammonia-N due to presence of tannins and phenols help retain dietary proteins and slow down the degradability of rumen proteins (Kholif et al. 2015, 2016). Rumen protozoa are thought to be the primary source of rumen ammonia due to bacterial protein consumption and proteolysis (Bhatta et al. 2012). Reduction in ammonia-N also may be due to the decrease in the protozoal and bacterial population which involved in degradation of proteins in ruminants (Newbold et al. 2004). ML may play a function in limiting ammonia by reducing ruminal protein breakdown and deamination and as well as rumen ammonia. Higher VFA and lower ruminal ammonia during ML feed showed increased in consumption of dietary nitrogen (Babiker et al. 2017). Increase in propionate production also represents a change in rumen fermentation to reduce methane emission (Polyorach et al. 2014). Moringa leaves silage increased the total gas production, acetate, propionate while reduced the ruminal protozoa population and methane production (Morsy et al. 2022).
PF which is the ratio of in vitro substrate truly digested to gas volume (Blümmel et al. 1997) theoretically varies from 2.75 to 4.41 the values of PF of all the three groups with ML were within the normal range indicating proper portioning of nutrient for microbial mass production. The increase in MBP (mg) in the current study might be due to higher fraction of CP, in concurrence with greater ruminal degradability of ML protein (Makkar and Becker 1997). It might also be due to the improvement of the rumen microbiome and stimulation of fermentation process by the fermentable N and available carbohydrates supplied by M. oleifera leaves. ML supplementation altered ruminal fermentation and reduced in vitro greenhouse gases production (Kholif et al. 2022). Present study revealed that supplementation of ML improved protein content, digestibility rate, microbial biomass and partial fraction and reduces methane gas emission.
Moringa oleifera leaves can be used as a protein basis in diet of goats under in vitro conditions. Supplementation of ML improved the nutrient digestibility, rumen fermentation parameters and corresponding decrease in methane production. Consequently, it can be concluded that M. oleifera leaf powder can be replaced up to 10–20% of concentrate as a protein source and for methane mitigation in ruminants. Still, further study on different animals with different concentration of ML is needed to validate/expand the results under in vitro and in vivo conditions.
Availability of data and materials
All data are presented in tables and figures within this manuscript.
Abbreviations
- ML:
-
Moringa leaves
- TDMD:
-
True dry matter digestibility
- PF:
-
Partial fraction
- CP:
-
Crude protein
- EE:
-
Ether extract
- OM:
-
Organic matter
- MBP:
-
Microbial biomass
- TMR:
-
Total mixed ration
References
Abbas RK, Elsharbasy FS, Fadlelmula AA (2018) Nutritional values of Moringa oleifera, total protein. amino acid, vitamins, minerals, carbohydrates, total fat and crude fiber, under the semi-arid conditions of Sudan. J Microb Biochem Technol 10:56–58. https://doi.org/10.4172/1948-5948.1000396
Abdel-Raheem SM, Hassan EH (2021) Effects of dietary inclusion of Moringa oleifera leaf meal on nutrient digestibility, rumen fermentation, ruminal enzyme activities and growth performance of buffalo calves. Saudi J Biol Sci 28:4430–4436. https://doi.org/10.1016/j.sjbs.2021.04.037
Abou-Elkhair R, Mahboub H, Sadek K, Ketkat S (2020) Effect of prepartum dietary energy source on goat maternal metabolic profile, neonatal performance, and economic profitability. J Adv Vet Anim Res 7:566–574. https://doi.org/10.5455/javar.2020.g454
Afzal A, Hussain T, Hameed A, Shahzad M, Mazhar MU, Yang G (2022) Dietary Moringa oleifera alters periparturient plasma and milk biochemical indicators and promotes productive performance in goats. Front Vet Sci 8:787719. https://doi.org/10.3389/fvets.2021.787719
Al-Juhaimi FY, Alsawmahi ON, Abdoun KA, Ghafoor K, Babiker EE (2020) Antioxidant potential of Moringa leaves for improvement of milk and serum quality of Aardi goats. South Afr J Bot 129:134–137. https://doi.org/10.1016/j.sajb.2019.03.022
Aregheore EM (2020) Intake and digestibility of Moringa oleifera–batiki grass mixtures by growing goats. Small Rumin Res 46:23–28. https://doi.org/10.1016/S0921-4488(02)00178-5
Association of Official Analytical Chemist (2005) Official methods of analysis, vol 222, 21st edn. Association of Official Analytical Chemists, Washington, DC
Babiker EE, Juhaimia FAL, Ghafoora K, Abdoun KA (2017) Comparative study on feeding value of Moringa leaves as a partial replacement for alfalfa hay in ewes and goats. Livest Sci 195:21–26. https://doi.org/10.1016/j.livsci.2016.11.010
Baccou JC, Lambert F, Sauvaire Y (1977) Spectrophotometric method for the determination of total steroidal sapogenin. Analyst 102:458–465
Bannink A, Kogut J, Dijkstra J, France J, Kebreab E, Van Vuuren AM, Tamminga S (2006) Estimation of the stoichiometry of volatile fatty acid production in the rumen of lactating cows. J Theor Biol 7(238):36–51. https://doi.org/10.1016/j.jtbi.2005.05.026
Bashar MK, Huque KS, Sarker NR, Sultana N (2020) Quality assessment and feeding impact of Moringa feed on intake, digestibility, enteric CH4 emission, rumen fermentation, and milk yield. J Adv Vet Anim Res 7:521–529. https://doi.org/10.5455/javar.2020.g449
Benetel G, Silva TDS, Fagundes GM, Welter KC, Melo FA, Lobo AAG, Muir JP, Bueno ICS (2022) Essential oils as in vitro ruminal fermentation manipulators to mitigate methane emission by beef cattle grazing tropical grasses. Molecules 27:2227. https://doi.org/10.3390/molecules27072227
Bhatta R, Saravanan M, Baruah KTL (2012) Nutrient content, in vitro ruminal fermentation characteristics and methane reduction potential of tropical tannin-containing leaves. J Sci Food Agric 92:2929–2935. https://doi.org/10.1002/jsfa.5703
Blümmel M, Makkar HPS, Becker K (1997) In vitro gas production—a technique revisited. J Anim Physiol Anim Nutr 77:24–34. https://doi.org/10.1111/j.1439-0396.1997.tb00734.x
Bodas R, Prieto N, García-González R, Andrés S, Giráldez FJ, López S (2012) Manipulation of rumen fermentation and methane production with plant secondary metabolites. Anim Feed Sci Technol 176:78–93. https://doi.org/10.1016/j.anifeedsci.2012.07.010
Brisibe EA, Umoren UE, Brisibe F, Magalhaes PM, Ferreira JFS, Luthria D, Wu X, Prior RL (2009) Nutritional characterization and antioxidant capacity of different tissues of Artemisia annua L. Food Chem 115:1240–1246. https://doi.org/10.1016/j.foodchem.2009.01.033
Choudhary S, Santra A, Muwel N, Sarkar S, Mandal A, Das SK (2022) Screening of forest tree leaves from North Eastern Himalayan region as feed additives for modulating in vitro rumen fermentation and methanogenesis from total mixed ration. Agroforest Syst 96:359–374. https://doi.org/10.1007/s10457-021-00724-5
Cohen-Zinder M, Leibovich H, Vaknin Y, Sagi G, Shabtay A, Ben-Meir Y (2016) Effect of feeding lactation cows with ensiled mixture of M. oleifera, wheat hay and molasses, on digestibility and efficiency of milk production. Anim Feed Sci Technol 211:75–83. https://doi.org/10.1016/j.anifeedsci.2015.11.002
Dey A, Paul SS, Pandey P, Rathore R (2014) Potential of Moringa oleifera leaves in modulating in vitro methanogenesis and fermentation of wheat straw in buffalo. Indian J Anim Sci 84:533–538
Dong L, Zhang T, Diao Q (2019) Effect of dietary supplementation of Moringa oleifera on the production performance and fecal methanogenic community of lactating dairy cows. Animals 9:262. https://doi.org/10.3390/ani9050262
Ebeid HM, Mengwei L, Kholif AE, Hassan FU, Lijuan P, **n L, Chengjian Y (2020) Moringa oleifera oil modulates rumen microflora to mediate in vitro fermentation kinetics and methanogenesis in total mix rations. Curr Microbiol. https://doi.org/10.1007/s00284-020-01935-2
Eisen MB, Brown PO (2022) Rapid global phaseout of animal agriculture has the potential to stabilize greenhouse gas levels for 30 years and offset 68 percent of CO2 emissions this century. PLOS Clim 1:e0000010. https://doi.org/10.1371/journal.pclm.0000010
Elghandour MMY, Vallejo LH, Salem AZM, Mellado M, Camacho LM, Cipriano M, Olafadehan OA, Olivares J, Rojas S (2017a) Moringa oleifera leaf meal as an environmental friendly protein source for ruminants: biomethane and carbon dioxide production, and fermentation characteristics. J Clean Prod. https://doi.org/10.1016/j.jclepro.2017.07.151
Elghandour MMY, Vázquez JC, Salem AZM, Kholif AE, Cipriano MM, Camacho LM, Márquez O (2017b) In vitro gas and methane production of two mixed rations influenced by three different cultures of Saccharomyces cerevisiae. J Appl Anim Res 45:389–395
Fadiyimu AA, Alokan JA, Fajemisin AN (2010) Digestibility, nitrogen balance and haematological profile of West African dwarf sheep fed dietary levels of Moringa oleifera as supplement to Panicum maximum. J Am Sci 6:634–643
Folin O, Ciocalteu V (1927) On tyrosine and tryptophane determinations in proteins. J Biol Chem 73:627–650. https://doi.org/10.1016/S0021-9258(18)84277-6
Goel G, Makkar HPS (2012) Methane mitigation from ruminants using tannins and saponins. Trop Anim Health Prod 4:729–739. https://doi.org/10.1007/s11250-011-9966-2
Gupta K, Barat GK, Wagle DS, Chawla HKL (1989) Nutrient contents and antinutritional factors in conventional and non-conventional leafy vegetables. Food Chem 31:105–116. https://doi.org/10.1016/0308-8146(89)90021-6
Gupta S, Mohini M, Thakur SS, Mondal G (2018) Effect of dietary monensin supplementation on nitrogen utilization and plasma metabolites in lactating Murrah buffaloes. Int J Curr Microbiol App Sci 7:3838–3845. https://doi.org/10.20546/ijcmas.2018.707.446
Kholif AE, Olafadehan OA (2021) Essential oils and phytogenic feed additives in ruminant diet: chemistry, ruminal microbiota and fermentation, feed utilization and productive performance. Phytochem Rev 20:1087–1108. https://doi.org/10.1007/s11101-021-09739-3
Kholif AE, Gouda GA, Morsy TA, Salem AZM, Lopez S, Kholif AM (2015) Moringa oleifera leaf meal as a protein source in lactating goat’s diets: feed intake, digestibility, ruminal fermentation, milk yield and composition, and its fatty acids profile. Small Rumin Res 129:129–137. https://doi.org/10.1016/j.smallrumres.2015.05.007
Kholif AE, Morsy TA, Gouda GA, Anele UY, Galyean ML (2016) Effect of feeding diets with processed Moringa oleifera meal as protein source in lactating Anglo-Nubian goats. Anim Feed Sci Technol 217:45–55. https://doi.org/10.1016/j.anifeedsci.2016.04.012
Kholif AE, Gouda GA, Anele UY, Galyean ML (2018) Extract of Moringa oleifera leaves improves feed utilization of lactating Nubian goats. Small Rumin Res 158:69–75. https://doi.org/10.1016/j.smallrumres.2017.10.014
Kholif AE, Gouda GA, Galyean ML, Anele UY, Morsy TA (2019) Extract of Moringa oleifera leaves increases milk production and enhances milk fatty acid profile of Nubian goats. Agroforest Syst 93:1877–1886. https://doi.org/10.1007/s10457-018-0292-9
Kholif AE, Gouda GA, Morsy TA, Matloup OH, Fahmy M, Gomaa AS, Patra AK (2022) Dietary date palm leaves ensiled with fibrolytic enzymes decreased methane production, and improved feed degradability and fermentation kinetics in a ruminal in vitro system. Waste Biomass Valor 13:3475–3488. https://doi.org/10.1007/s12649-022-01752-7
Komolong MK, Barber DG, McNeill DM (2001) Post-ruminal protein supply and N retention of weaner sheep fed on a basal diet of lucerne hay (Medicago sativa) with increasing levels of quebracho tannins. Anim Feed Sci Technol 92:59–72. https://doi.org/10.1016/S0377-8401(01)00246-2
Ku-Vera JC, Jiménez-Ocampo R, Valencia-Salazar SS, Montoya-Flores MD, Molina-Botero IC, Arango J, Gómez-Bravo CA, Aguilar-Pérez CF, Solorio-Sánchez FJ (2020) Role of secondary plant metabolites on enteric methane mitigation in ruminants. Front Vet Sci 7:584. https://doi.org/10.3389/fvets.2020.00584
Li Y, Zhang G-N, Xu H-J, Zhou S, Dou X-Z, Lin C, Zhang X-Y, Zhao H-B, Zhang Y-G (2019) Effects of replacing alfalfa hay with Moringa oleifera leaves and peduncles on intake, digestibility, and rumen fermentation in dairy cows. Livest Sci 220:211–216. https://doi.org/10.1016/j.livsci.2019.01.005
Machmüller A, Ossowski DA, Kreuzer M (2000) Comparative evaluation of the effects of coconut oil, oilseeds and crystalline fat on methane release, digestion and energy balance in lambs. Anim Feed Sci Technol 85:41–60. https://doi.org/10.1016/S0377-8401(00)00126-7
Makkar HPS, Becker K (1996) Nutritional value and antinutritional components of whole and ethanol extracted Moringa oleifera leaves. Anim Feed Sci Technol 63:211–228. https://doi.org/10.1016/S0377-8401(96)01023-1
Makkar HPS, Becker K (1997) Nutrients and antiquality factors in different morphological parts of the Moringa oleifera tree. J Agric Sci 128:311–322
Menke KH, Steingass H (1988) Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Anim Res Dev 28:7–55
Morsy TA, Gouda GA, Kholif AE (2022) In vitro fermentation and production of methane and carbon dioxide from rations containing Moringa oleifera leave silage as a replacement of soybean meal: in vitro assessment. Environ Sci Pollut Res. https://doi.org/10.1007/s11356-022-20622-2
Newbold CJ, McIntosh FM, Williams P, Losa R, Wallace RJ (2004) Effects of a specific blend of essential oil compounds on rumen fermentation. Anim Feed Sci Technol 114:105–112
Nwinuka NM, Ibeh GO, Ekeke GI (2005) Proximate composition and levels of some toxicants in four commonly consumed spices. J Appl Sci Environ Manag 9:150–155
Parra-Garcia A, Elghandour MMY, Greiner R, Barbabosa-Pliego A, Camacho-Diaz LM, Salem AZM (2019) Effects of Moringa oleifera leaf extract on ruminal methane and carbon dioxide production and fermentation kinetics in a steer model. Environ Sci Pollut Res 26:15333–15344. https://doi.org/10.1007/s11356-019-04963-z
Polyorach S, Wanapat M, Cherdthong A (2014) Influence of yeast fermented cassava chip protein (YEFECAP) and roughage to concentrate ratio on ruminal fermentation and microorganisms using in vitro gas production technique. Asian Australas J Anim Sci 27:36–46. https://doi.org/10.5713/ajas.2013.13298
Saleem A, Saleem M, Akhtar MF (2020) Antioxidant, anti-inflammatory and antiarthritic potential of Moringa oleifera Lam: an ethnomedicinal plant of Moringaceae family. South Afr J Bot 128:246–256. https://doi.org/10.1016/j.sajb.2019.11.023
Soliva CR, Kreuzer M, Foidl N, Foidl G, Machmüller A, Hess HD (2015) Feeding value of whole and extracted Moringa oleifera leaves for ruminants and their effects on ruminal fermentation in vitro. Anim Feed Sci Tech 118(2):47–62. https://doi.org/10.1016/j.anifeedsci.2004.10.005
Su B, Chen X (2020) Current status and potential of Moringa oleifera leaf as an alternative protein source for animal feeds. Front Vet Sci 7:53. https://doi.org/10.3389/fvets.2020.00053
Sultana S (2020) Nutritional and functional properties of Moringa oleifera. Metabol Open 8:100061. https://doi.org/10.1016/j.metop.2020.100061
Sultana N, Alimon A, Huque K, Sazili A, Yaakub H, Hossain J, Baba M (2015) The feeding value of Moringa (Moringa oleifera) foliage as replacement to conventional concentrate diet in Bengal goats. Adv Anim Vet Sci 3:164–173
Sultana N, Das NG, Kabir MA, Deb GK, Islam MT (2021) Metabolic benefit of bulls being fed Moringa leaves twigs and branches as a major concentrate ingredient. Front Anim Sci 2:712919. https://doi.org/10.3389/fanim.2021.712919
Teclegeorgish ZW, Aphane YM, Mokgalaka NS, Steenkamp P, Tembu VJ (2021) Nutrients, secondary metabolites and anti-oxidant activity of Moringa oleifera leaves and moringa-based commercial products. South Afr J Bot 142:409–420. https://doi.org/10.1016/j.sajb.2021.07.008
Teixeira EM, Carvalho MR, Neves VA, Silva MA, Arantes-Pereira L (2014) Chemical characteristics and fractionation of proteins from Moringa oleifera Lam. Leaves. Food Chem 147:51–54. https://doi.org/10.1016/j.foodchem.2013.09.135
Van Soest PJ, Robertson JB, Lewis BA (1991) Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci 74:3583–3597. https://doi.org/10.3168/jds.S0022-0302(91)78551-2
Wankhede SD, Dutta N, Tambe MB, Kaur N, Jadhav SE, Pattanaik AK (2022) Effect of dietary inclusion of Moringa oleifera foliage on nutrient metabolism, metabolic profile, immunity and growth performance of goat kids. Emerging Animal Species 3:100005. https://doi.org/10.1016/j.eas.2022.100005
Zeru AE, Hassen A, Apostolides Z, Tjelele J (2022) Screening of candidate bioactive secondary plant metabolite ion-features from Moringa oleifera accessions associated with high and low enteric methane inhibition from ruminants. Metabolites 12:501. https://doi.org/10.3390/metabo12060501
Zhishen J, Mengcheng T, Jianming W (1999) The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 64:555–559. https://doi.org/10.1016/S0308-8146(98)00102-2
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We thank the Director, National Dairy Research Institute, Karnal, Haryana, India for providing facilities to conduct this research.
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VKL performed all experiments and data analysis, PC wrote and editing the manuscript, MK helped in data analysis, MM conceptualization and editing the manuscript, GM experimental concept, design and final editing of the manuscript. All authors read and approved the final manuscript.
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Leitanthem, V.K., Chaudhary, P., Bhakat, M. et al. Impact of Moringa oleifera on rumen fermentation and methane emission under in vitro condition. AMB Expr 12, 141 (2022). https://doi.org/10.1186/s13568-022-01480-0
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DOI: https://doi.org/10.1186/s13568-022-01480-0