Ogt-mediated O-GlcNAcylation inhibits astrocytes activation through modulating NF-κB signaling pathway

Dong, **aoxue; Shu, Liqi; Zhang, **yu; Yang, Xu; Cheng, Xuejun; Zhao, **ngsen; Qu, Wenzheng; Zhu, Qiang; Shou, Yikai; Peng, Guo**; Sun, Binggui; Yi, Wen; Shu, Qiang; Li, Xuekun

doi:10.1186/s12974-023-02824-8

Ogt-mediated O-GlcNAcylation inhibits astrocytes activation through modulating NF-κB signaling pathway

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Published: 22 June 2023

Volume 20, article number 146, (2023)
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Ogt-mediated O-GlcNAcylation inhibits astrocytes activation through modulating NF-κB signaling pathway

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Guo** Peng⁵,
Binggui Sun^1,6,
Wen Yi⁴,
Qiang Shu¹ &
…
Xuekun Li^1,2,7,8

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Abstract

Previous studies have shown that Ogt-mediated O-GlcNAcylation is essential for neuronal development and function. However, the function of O-GlcNAc transferase (Ogt) and O-GlcNAcylation in astrocytes remains largely unknown. Here we show that Ogt deficiency induces inflammatory activation of astrocytes in vivo and in vitro, and impairs cognitive function of mice. The restoration of O-GlcNAcylation via GlcNAc supplementation inhibits the activation of astrocytes, inflammation and improves the impaired cognitive function of Ogt deficient mice. Mechanistically, Ogt interacts with NF-κB p65 and catalyzes the O-GlcNAcylation of NF-κB p65 in astrocytes. Ogt deficiency induces the activation of NF-κB signaling pathway by promoting Gsk3β binding. Moreover, Ogt depletion induces the activation of astrocytes derived from human induced pluripotent stem cells. The restoration of O-GlcNAcylation inhibits the activation of astrocytes, inflammation and reduces Aβ plaque of AD mice in vitro and in vivo. Collectively, our study reveals a critical function of Ogt-mediated O-GlcNAcylation in astrocytes through regulating NF-κB signaling pathway.

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Introduction

Astrocytes are abundant glial cells in central nervous system (CNS) and play critical function, such as maintaining CNS homeostasis, regulating synaptic information processing [1, 25, 39, 58]. Astrocytes activation, marked by the up-regulation of glial fibrillary acidic protein (GFAP) [60], presents in some pathological conditions including neurodegenerative diseases [16, 23, 44]. Reactive astrocytes secrete pro-inflammatory cytokines affecting neuronal survival and consequently contribute to neurological diseases such as Alzheimer’s disease, multiple sclerosis and spinal cord injury [6, 10, 22, 45, 46, 54, 71]. Diverse mechanisms, including epigenetic modifications and environmental factors, have been shown regulate the activation of astrocytes [46, 61, 71].

As a post-translational modification, O-GlcNAcylation is catalyzed by O-GlcNAc transferase (Ogt) with UDP-GlcNAc on the serine and threonine residues of proteins; meanwhile, O-GlcNAcylation can be removed by O-GlcNAcase (Oga). The balance between the activities of Ogt and Oga is essential for the dynamic and reversible feature of O-GlcNAcylation. Previous studies have shown that thousands of cytoplasmic and nuclear proteins are modified with O-GlcNAcylation, which is implicated in gene expression, proteasomal degradation, energy metabolism, cellular stress responses, and signal transduction [24, 26, 40, 64, 75].

Previous studies have shown that neuronal Ogt is essential for gene expression, neuronal survival and synaptic development, and that Ogt deficiency leads to abnormal feeding behavior and induces neurodegeneration [29, 36, 37, 55, 62, 67]. Specific deletion of Ogt in neural stem/progenitor cells alters neurogenesis and impairs cognitive function of mice [7, 9, 72]. Consistently, global level of O-GlcNAcylation decreases along with brain ageing, and its restoration can attenuate cognitive impairment in aged mice [70]. However, the function of O-GlcNAcylation in astrocytes remains largely unknown.

In the present study, we showed that astrocytic Ogt deficiency induced activation of astrocytes in vivo and in vitro and impaired cognitive function of mice. The restoration of O-GlcNAcylation by the replenishment of GlcNAc increased the level of UDP-O-GlcNAcylation, inhibited activation of astrocytes, and improved the cognitive function of Ogt deficient mice. Mechanistically, Ogt interacted with NF-κB p65 and catalyzed the O-GlcNAcylation of NF-κB p65 in astrocytes. Ogt deficiency promoted the binding of Gsk3β to NF-κB p65 and led to the activation of NF-κB signaling pathway. The restoration of decreased O-GlcNAcylation inhibited activation of astrocytes, inflammation and reduced Aβ plaque in the brain of Alzheimer’s disease (AD) mouse model.

Results

Ogt deficiency leads to the activation of astrocytes in vitro and in vivo

To determine the function of Ogt in astrocytes, we first isolated astrocytes from the brains of wild-type (WT) adult (postnatal 7-week) mice and performed immunostaining with multiple cell lineage markers. We observed that nearly 97% of cells were positive for astrocyte markers GFAP, Glast and Aldh1l1, respectively, but only very few cells were positive for the neuronal cell marker Tuj1 and the microglia marker Iba1 (Additional file 1: Fig. S1a, b), suggesting the homogeneity of cultured astrocytes. Western blot (WB) assay results showed that astrocytes displayed lower levels of Ogt and Oga compared to neurons (Additional file 1: Fig. S1c–e). However, the ratio of Ogt/Oga was significantly higher in astrocytes than that of neurons (Additional file 1: Fig. S1c, f). Consistently, the level of O-GlcNAcylation was significantly higher in astrocytes compared to neurons (Additional file 1: Fig. S1c, g).

Next, we generated a GlastCreERT2::Ogt^loxp/Y (cKO) mouse model by crossing Ogt^loxp/loxp conditional allele with GlastCreERT2 driver. Adult (postnatal 7-week) male mice were intraperitoneally (i.p.) administrated with multiple doses of tamoxifen (cKO) and corn oil (Ctrl) (1 time/day for 5 consecutive days, Additional file 1: Fig. S1h), respectively. Eight weeks after the final injection, astrocytes were isolated from the brains of Ctrl and cKO mice for in vitro assays, respectively. Both mRNA and protein levels of Ogt (Additional file 1: Fig. S1i–k), and the levels of O-GlcNAcylation and Oga (Additional file 1: Fig. S1j–m) were also significantly decreased in cKO cells compared to Ctrl cells. Immunostaining intensity of Ogt and O-GlcNAcylation signals were significantly decreased compared to those of Ctrl cells in vitro (Additional file 1: Fig. S1n, o). Interestingly, we noticed that the average area of cKO astrocytes (indicated by GFAP staining), the signal intensity and the protein level of GFAP were remarkably increased compared to Ctrl cells (Fig. 1a–d). mRNA levels of pan reactive astrocyte markers were also significantly increased in cKO astrocytes (Fig. 1e). Of note, mRNA levels of A1 type (neurotoxic) astrocyte markers were significantly increased; whereas, mRNA levels of A2 type (neuroprotective) astrocytic markers showed a subtle decrease (Fig. 1f, g). These results collectively suggest that Ogt deficiency leads to the activation of astrocytes in vitro.

Next, we aim to examine whether Ogt deficiency induces the activation of astrocytes in vivo. Immunostaining with brain sections revealed that Ogt and O-GlcNAcylation were significantly decreased in GFAP⁺ astrocytes of cKO mice (Additional file 1: Fig. S1p, q). Immunostaining intensity of GFAP and the number of astrocytes significantly increased in the hippocampal region of cKO mice compared to Ctrl mice (Fig. 1h–j). Three-dimension (3D) construction analysis showed that the volume, the number and process length of astrocytes significantly increased in the brain of cKO mice compared to Ctrl (Fig. 1k–o, Additional file 2: Fig. S2a), while the number and neurite length of microglia were not affected in the brain of mice (Additional file 2: Fig. S2b–g). Consistently, qRT-PCR and western blot results revealed a significant increase of GFAP but not Iba1 in the hippocampal tissue of cKO mice compared to Ctrl mice (Fig. 1p–s, Additional file 2: Fig. S2h).

Considering that few Glast⁺ astrocytes could be Nestin⁺ adult neural stem/progenitor cells (aNSPCs) in the brain of adult mice, we then examined whether Ogt deficiency in astrocytes affected neurogenesis. We observed that adult cKO mice showed the reduced number of BrdU⁺ cells and BrdU⁺DCX⁺ cells in the subgranular zone of hippocampus (Additional file 3: Fig. S3a–c) and subventricular zone (Additional file 3: Fig. S3e–g) compared to Ctrl, respectively, but the percentage of BrdU⁺DCX⁺/BrdU⁺ in these two regions showed no difference between Ctrl and cKO mice (Additional file 3: Fig. S3a, d, e, h). We further isolated aNSPCs from the forebrains of adult Ctrl and cKO mice, respectively. Ctrl and cKO NSPCs showed no difference in the levels of Ogt and O-GlcNAcylation (Additional file 3: Fig. S3i–k), and mRNA levels of proliferation and differentiation markers (Additional file 3: Fig. S3l–q). In addition, we did not observe the activation of astrocytes in the brain of NestinCreERT2::Ogt^loxp/Y mice, in which Ogt is specific deficient in Nestin⁺ aNSPCs (Additional file 3: Fig. S3r, s). Collectively, these results suggest that Ogt deficiency in astrocytes specifically leads to astrocyte activation and impairs neurogenesis in vivo.

Activated astrocytes induce inflammation in Ogt deficient mice

To determine whether activated astrocytes induced by Ogt deficiency drives inflammation, we first performed IL-1β immunostaining with cultured Ctrl and cKO astrocytes, respectively, and observed that the intensity of IL-1β signal increased in cKO astrocytes compared to Ctrl (Fig. 2a). qRT-PCR and WB results also showed that the levels of IL-1β and TNF-αwere significantly increased in cKO astrocytes (Fig. 2b–f).

Next, we examined the expression of IL-1β in the brains of Ctrl and cKO mice, and observed that the immunofluorescence intensity of IL-1β signal was remarkably increased (Fig. 2g). Consistently, qRT-PCR (Fig. 2h, i) and WB assays (Fig. 2j–l) results showed that the levels of IL-1β and TNF-α were significantly increased in the hippocampal tissues of cKO mice compared to Ctrl mice. ELISA results showed that the levels of IL-1β and TNF-α were significantly increased in the hippocampal tissue supernatants of cKO mice compared to Ctrl mice (Fig. 2m, n). Further, the exposure of an Ogt inhibitor, Ogt small molecule inhibitor-4 (OSMI-4), significantly reduced the level of O-GlcNAcylation in wild-type (WT) astrocytes (Fig. 2o, p), but significantly increased the of protein level of GFAP (Fig. 2o, q). In addition, the treatment with OSMI-4 also remarkably increased the staining intensity of GFAP and IL-1β (Fig. 2r), and the protein levels of IL-1β and TNF-α (Fig. 2s–u). These results collectively suggest that Ogt deficiency and inhibition of Ogt activity induce astrocytes activation and drive inflammation in vitro and in vivo.

Ogt deficiency in astrocytes impairs neurons and cognitive function of mice

Next, we examined the effects of Ogt deficiency in astrocytes on neuronal cells. ELISA results showed that the levels of IL-1β and TNF-α significantly increased in the supernatants of cultured cKO astrocytes (Additional file 4: Fig. S4a, b). The supplementation of supernatants from cKO astrocytes significantly reduced the intersection number and dendritic length of cultured hippocampal neurons (Additional file 4: Fig. S4c–f). In addition, the treatment with the supernatants of cultured cKO astrocytes also significantly increased the number of active Caspase3 positive (aCaspase3⁺) cells (Additional file 4: Fig. S4g, h) and the protein level of aCaspase3 (Additional file 4: Fig. S4i, j). Golgi staining results showed that hippocampal neurons displayed shorter length of dendrites, decreased numbers of intersections and spines in the brain of Ogt deficient mice (Fig. 3a–f). Together, these results suggest that activated astrocytes induce inflammation and impair neurons in Ogt deficient mice.

Given the neurons were impaired in cKO mice, we next examined whether Ogt deficiency in astrocytes affects cognition of mice. Eight weeks after tamoxifen induction (Additional file 4: Fig. S4k), Morris water maze (MWM) test was performed and we observed that cKO mice displayed longer latency during the training period (Fig. 3g, Additional file 4: Fig. S4l). A probe test was performed 24 h after the training. Although Ctrl and cKO mice exhibited similar swimming speed and travelled paths (Additional file 4: Fig. S4m, n), cKO mice displayed the longer latency, the decreased time in the target quadrant and decreased numbers of crossing the platform (Fig. 3h–j). Y maze test results showed that cKO mice displayed the lower percentage of spontaneous alternations than Ctrl mice during the trial test (Fig. 3k), while Ctrl and Ogt cKO mice showed no difference between the times of arm entries and distance length (Additional file 4: Fig. S4o, p). For the spatial novelty preference test in the Y maze, cKO mice displayed a lower percentage of time exploring in the novel arm, decreased distance and numbers of arm entries compared to those of Ctrl mice (Fig. 3l–o). Further, cKO mice displayed shorter latency to enter the dark compartment than that of Ctrl mice during the retention test, while it showed no difference between groups during the training session in the passive avoidance task (Fig. 3p). Collectively, these results suggest that cKO mice have the impaired learning and memory.

Restoration of O-GlcNAcylation inhibits astrocyte reactivation and inflammation

Previous studies have shown that N-acetylglucosamine (GlcNAc) could be converted to UDP-GlcNAc, the substrate of O-GlcNAcylation [3, 31, 57]. Next, we examined whether the restoration of O-GlcNAcylation via GlcNAc administration could inhibit astrocytes activation and inflammation in vitro. Ctrl and Ogt-deficient astrocytes were treated with phosphate buffer solution (PBS, Ctrl) and GlcNAc (20 μM) for 72 h, respectively. ELISA results showed that GlcNAc supplementation led to a significant increase of UDP-GlcNAc level (Fig. 4a). GlcNAc supplementation also reduced the staining intensity of GFAP and IL-1β of the cultured cKO astrocytes (Fig. 4b). WB assay and quantification results showed that GlcNAc supplementation significantly restored the level of O-GlcNAcylation (Fig. 4c, d) and remarkably reduced the levels of GFAP, IL-1β and TNF-α in cultured astrocytes (Fig. 4c, e–g).

Next, we examined the effects of the GlcNAc supplementation on astrocyte activation in vivo. Ctrl and cKO mice were administrated with saline and GlcNAc (400 mg/kg, i.p., 1 time/day for 14 days), respectively (Additional file 5: Fig. S5a). ELISA assay results showed that the level of UDP-GlcNAc was significantly increased in the supernatants of hippocampal tissue of cKO mice (Fig. 4h). ELISA assay results also showed that the levels of IL-1β and TNF-α were significantly decreased in the hippocampal tissue supernatants of GlcNAc-treated cKO mice compared to those of saline-treated cKO mice (Fig. 4i, j). Immunostaining showed that GlcNAc supplementation increased the intensity of O-GlcNAcylation in the brain of cKO mice (Fig. 4k, l). WB results showed that the GlcNAc supplementation significantly reduced the levels of GFAP, IL-1β and TNF-α in the hippocampal tissues of cKO mice (Fig. 4m–p). GlcNAc supplementation also remarkably reduced the immunostaining intensities of GFAP and IL-1β in the brain of cKO mice (Additional file 5: Fig. S5b). Taken together, these results suggest that the administration of GlcNAc restores O-GlcNAcylation, and inhibits astrocyte activation and inflammation in vitro and in vivo.

Restoration of O-GlcNAcylation improves behavioral deficits of Ogt deficient mice

To examine whether the restoration of O-GlcNAcylation could improve the behavioral deficits of cKO mice, Ctrl and cKO mice were administrated with saline and GlcNAc for 14 days (400 mg/kg, i.p., 1 time/day), respectively, and a series of behavioral tests were performed (Additional file 5: Fig. S5c). A novel object recognition task test showed no difference in exploration time between groups during the training time (day 1) (Additional file 5: Fig. S5d), but cKO mice treated with GlcNAc displayed a longer time of exploring novel objects compared to the saline-treated cKO mice during the test (day 2) (Fig. 5a, b, Additional file 5: Fig. S5e). Y maze test results showed that GlcNAc-treated cKO mice showed a significant increase in the percentage of spontaneous alternation compared to saline-treated cKO mice (Fig. 5c). No difference was observed for the times of arm entries and the travelled distance between groups (Additional file 6: Fig. S6a, b). Y maze also showed that GlcNAc-treated cKO mice displayed the longer time, longer distance and higher numbers of entries to the novel arm compared to saline-treated cKO mice (Fig. 5d–g, Additional file 6: Fig. S6c).

Next, we performed Morris Water Maze test (MWM) and observed that cKO mice treated with the GlcNAc displayed shorter latency compared to saline-treated cKO mice during the training period (Fig. 5h). 24 h after training, the probe test results showed that cKO mice treated with GlcNAc exhibited the reduced latency, increased time in target quadrant and increased numbers of crossing the platform compared to saline-treated cKO mice (Fig. 5i–k). GlcNAc supplementation did not affect the swimming speed and travelled path of cKO mice (Additional file 6: Fig. S6d–f). Collectively, these results suggest that the restoration of GlcNAcylation improves the behavioral deficits of cKO mice.

Ogt catalyzes the O-GlcNAcylation of NF-κB p65 and inhibits the activation of NF-κB signaling pathway

Previous studies have shown the important function of the NF-κB (p65) signaling pathway in inflammation [5, 11, 30, 35, 38, 59, 79]. Next, we examined whether Ogt deficiency induced inflammation through regulating NF-κB signaling pathway. co-immunoprecipitation (co-IP) showed that Ogt directly interacts with NF-κB p65, and vice versa, in cultured astrocytes (Fig. 6a, b). A pan-O-GlcNAcylation antibody could also precipitate p65, and vice versa, in cultured astrocytes (Fig. 6c, d). Chemoenzymatic labeling assay [32, 43] results showed that p65 was modified with O-GlcNAcylation (Fig. 6e). Under Ogt knock down (KD) condition, IP followed by WB assays showed that Ogt KD significantly decreased the O-GlcNAcylation of p65 (p65-HA), but significantly increased the level of p-p65, an active form of p65, in N2a cells (Fig. 6f–h). Consistently, IP followed by WB assays showed that ectopic Ogt significantly increased the O-GlcNAcylation of p65, but decreased the level of p-p65 in N2a cells (Fig. 6i–k).

To uncover the O-GlcNAcylation sites of p65, ectopic expression of p65 were performed followed by O-GlcNAcylation immunoprecipitation (IP) in N2a cells, and the precipitates were subjected to liquid chromatography coupled tandem mass spectrometry (LC–MS/MS). Four potential O-GlcNAcylation sites, Ser370, Ser374, Ser384, and Ser396 were identified on p65 (Additional file 7: Fig. S7a). Among these sites, the O-GlcNAcylation of Ser370, Ser384, and Ser396 had not been reported previously. The mutation of each single site (S370A, S374A, S384A, or S396A) led to a significant decrease of the O-GlcNAcylation level of p65, with the mutation of S384A and S396A showing the most dramatic effect (Additional file 7: Fig. S7b, c). Of note, only the mutation of S384A induced a significant increase of p-p65 level (Additional file 7: Fig. S7b, d).

To identify the proteins which phosphorylated p65, hippocampal tissue from Ctrl and cKO mice were performed IP with p65 antibody and the precipitates were subjected to liquid chromatography coupled tandem mass spectrometry (LC–MS/MS). MS/MS data analysis revealed 392 and 458 proteins in the precipitates of Ctrl and cKO mice, respectively (Additional file 11: Table S1). Of note, glycogen synthase kinase 3β (GSK3β) was specifically identified in cKO group. GSK3β has kinase activity and phosphorylates NF-κB p65 [65]. Co-IP with hippocampal tissues of Ctrl and cKO mice showed that the interaction between p65-GSK3β significantly increased in AD mice compared to Ctrl (Additional file 7: Fig. S7e–i). IP followed by WB assays showed that p65 directly interacted with glycogen synthase kinase 3β (Gsk3β), and Ogt KD promoted the binding of Gsk3β to p65 (Fig. 6l–n). Further, S384A mutation of Ogt significantly increased the binding of Gsk3β to p65 compared to the mutations of other O-GlcNAcylation sites (Fig. 6o–q). Of note, we did not observe a direct interaction between Gsk3β and Ogt (Additional file 7: Fig. S7j).

Next, we performed RNA-seq with Ctrl and Ogt cKO astrocytes. RNA-seq data analysis identified that 4269 genes showed differential expression, with 2343 genes up-regulated and 1926 genes down-regulated (Additional file 8: Fig. S8a, Additional file 12: Table S2). Gene ontology (GO) analysis revealed that up-regulated genes enriched terms for inflammatory response, cytokine signaling and NF-κB signaling pathway; whereas, down-regulated genes enriched for axon development and learning and memory (Additional file 8: Fig. S8b). WB results showed that the level of total p65 was not altered, but the level of p-p65 was significantly increased in cKO astrocytes compared to that of Ctrl cells (Additional file 8: Fig. S8c–e). In addition, we observed that cytoplasmic p65 was significantly decreased (Additional file 8: Fig. S8f, g) and nucleic p-p65 significantly increased at protein levels (Additional file 8: Fig. S8h, i). These results collectively suggest that Ogt regulates NF-κB signaling pathway through catalyzing O-GlcNAcylation on S384 of NF-κB p65.

Ogt depletion induces inflammation by activating NF-κB signaling in human astrocytes

Next, we aim to examine whether Ogt deficiency also induces inflammation in human astrocytes. Induced pluripotent stem cells (iPSCs) were generated by reprogramming urinary cells with Sendai virus following the manufacturer’s protocol, and were induced differentiation towards astrocyte lineage. Astrocytes were infected with lentivirus expressing shRNA against human Ogt. We observed that Ogt KD significantly reduced Ogt level, and remarkably increased mRNA levels of GFAP, IL-1β and TNF-α in human astrocytes (Fig. 7a–d). Ogt KD significantly reduced the levels of Ogt protein and O-GlcNAcylation (Fig. 7e–g), but remarkably increased the protein level of TNF-α in human astrocytes, while the level of IL-1β showed no difference (Fig. 7h–j). In addition, WB results showed that Ogt KD significantly increased the level of nuclear p-p65 in astrocytes, but did not affect the level of cytoplasmic p-65 (Fig. 7k–n). These results suggest that Ogt deficiency induces human astrocytes activation and inflammation.

Restoration of O-GlcNAcylation inhibits inflammatory activation of AD astrocytes

Finally, we aim to examine the roles of O-GlcNAcylation in Alzheimer’s disease (AD) mouse model. Previous studies have shown the decreased O-GlcNAcylation and the activation of astrocytes in the brain of the AD mouse model and AD patients [52, 53, 56, 69, 81]. Briefly, O-GlcNAcylated proteins in cell lysates were first labeled with GalNAz using the Click-iT O-GlcNAc Enzymatic Labeling System and conjugated with an alkyne-biotin compound using the Click-iT Protein Analysis Detection System. Control experiments in the absence of the enzyme GalT or UDP-GalNAz were carried out in parallel. Labeled proteins were then precipitated, resolubilized, neutralized, and further incubated with streptavidin beads at 4 °C overnight. After extensive washing steps, the bound protein fractions were eluted in the boiling elution buffer containing 50 mM Tris–HCl pH 6.8, 2.5% SDS, 100 mM DTT, 10% glycerol, and 20 mM biotin. Immunoblotting was carried out using anti-p65 antibody.

Flag-tagged p65 and HA-tagged OGT were co-expressed in N2a cells for 48 h. Flag-tagged p65 was immunoprecipitated from the cell lysates using anti-Flag-M2 magnetic beads and eluted in a buffer containing 4% SDS and 100 mM Tris–HCl pH 8.0. After separation and staining in 8–15% Bis–Tris gels, the p65 protein band was excised and digested with trypsin and chymotrypsin. The extracted mixtures were purified by reverse-phase HPLC (Agilent 1100) using a gradient of 5–30% buffer B (100% MeCN) over 20 min at 4 ml/min and a constant flow of buffer A (0.5% aqueous AcOH). Eluted fractions between 5 and 12 min were manually collected, pooled, lyophilized, and analyzed using nanoLC-LTQ-CID/ETD-MS. Proteome Discovery (MASCOT search engine, version 1.3) was used for data search with O-GlcNAc (Ser/Thr) set as a variable modification.

Immunoprecipitation/co-immunoprecipitation (IP/co-IP)

Primary P1 astrocytes were prepared for endogenous IP and Co-IP. N2a cells transfected with Ogt-Flag and NF-κB p65-HA overexpression plasmids were prepared for exogenous IP/Co-IP. For IP, cells were lysed by RIPA Lysis Buffer and lysates were filled up to 300 μl with IP Lysis Buffer (50 mM Tris–HCl, 0.5% Triton-X, 150 mM KCl, 1 mM EDTA in ddH₂O). For endogenous assay, lysates were blocked with 10 μl Protein A agarose beads at 4 °C for 2 h with rotation. 3 μl of primary antibody or rabbit IgG were added to the supernatant and incubated with rotation overnight at 4 °C. 30 μl of Protein A agarose beads were conjugated with antibody at 4 °C for 6–8 h with rotation. For exogenous assays, HA/Flag-beads with conjugated antibodies were added to the lysates directly at 4 °C overnight. Unconjugated protein was removed from beads by 3 washing times with IP Wash Buffer (50 mM Tris–HCl, 0.1% Triton-X, 150 mM KCl, 1 mM EDTA in ddH₂O) at 4 °C for 10 min. Target proteins were eluted from beads in 1× loading buffer at 100 °C for 5 min, and lysates before IP were used as the input. Proteins were detected by Western blot.

For co-IP assays, N2a cells and P0 astrocytes were crosslinked with 1% formaldehyde for 10 min, respectively, and the reaction was terminated with 125 mM glycine for 5 min at room temperature on a shaker at 40 rpm. Crosslinked cells were lysed with IP Lysis Buffer to avoid breaking up protein–protein interactions. co-IP assays were preformed similar to IP assay described above.

Total RNA extraction and quantitative real-time PCR (qRT-PCR)

Total RNA from cells and tissues were extracted by TRIzol reagent according to the manufacture’s protocol, and the concentration was determined with a Nanodrop spectrophotometer 2000 (Thermo Fisher Scientific). 0.5 µg total RNA was used for reverse transcription, and standard real-time qPCR assays were performed using SYBR Green (Vazyme) in triplicate. The results were analyzed using the ^ΔΔCt method. Samples from 3 to 5 animals per group were analyzed. The primers for qRT-PCR are shown in Additional file 14: Table S4.

RNA-seq and data analysis

All samples used for the cDNA library were assessed by NanoDrop 2000 (Thermo Fisher Scientific), and the RNA integrity value (RIN) was determined with the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies Inc.). A total amount of 3 µg of RNA per sample was used as input for each RNA sample preparation. Sequencing libraries were generated following the manufacturer’s recommendations, and index codes were added to attribute sequences to each sample using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB). Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and RNase H. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. The NEBNext Adaptor with hairpin loop structure was ligated to prepare for hybridization after adenylation of the 3′ ends of DNA fragments. The library fragments were purified with AMPure XP system (Beckman Coulter) to preferentially select cDNA fragments of 250–300 bp. Then, 3 µl USER Enzyme (NEB) was used with size-selected, adaptor-ligated cDNA at 37 °C for 15 min, followed by 5 min at 95 °C before PCR. Then PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer. Finally, PCR products were purified using AMPure XP system (Beckman Coulter), and library quality was assessed on the Agilent Bioanalyzer 2100 system (Agilent Technologies Inc.). Subsequently, the clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumina) according to the manufacturer’s instructions. After cluster generation, the library preparations were sequenced on an Illumina Hiseq platform (Illumina NovaSeq6000), and 125 bp/150 bp paired-end reads were generated.

Raw reads of fastq format were processed. In this step, clean reads were obtained by removing reads containing adapter, reads containing ploy-N and low-quality reads. The retained clean reads were aligned to the Mus musculus reference genome mm10. To quantify the gene expression level, feature counts v1.5.0-p3 was used to count the read numbers mapped to each gene. FPKM (expected number of Fragments Per Kilobase of transcript sequence per Millions base pairs sequenced) of each transcript was calculated based on the length of the gene and the count of reads mapped to this gene.

Gene ontology analysis

Gene ontology (GO) analysis was performed using the DAVID database, as described previously [8]. Each enriched GO function term is represented by a node, and the node size is proportional to the number of genes in its corresponding function term in the enrichment maps. Similar GO functions are categorized as one cluster. The function term and the number of genes in each cluster are labelled.

Behavioral tests

The Morris water maze (MWM) was performed as previously described [42]. MWM was performed in a round, water-filled tub (120 cm in diameter). MWM was divided into two stages: training and testing. For the training stage, an invisible escape platform (10 cm in diameter) was located in the same spatial location 1 cm below the water surface independent of a subject’s start position on a particular trial. Mice were placed in the water maze from 4 different starting positions (NE, NW, SE, SW). In this way, each animal would be able to determine the platform’s location, and each animal was given 4 trials/day for 4 days. 24 h after the final training trial, a probe trial was performed, during which the platform was removed and the time of swimming in the quadrant that previously placed the escape platform during task acquisition was measured over 60 s. All trials were videotaped and analyzed with MazeScan software (Acimetrics).

The Novel Object recognition (NOR) task test was performed as described previously [4]. Two sample objects in one environment were used to examine learning and memory with 24 h delays. In this test, mice were placed in a white plastic box (60 cm × 60 cm × 30 cm) with illumination, monitored by an overhead video camera and analyzed by an automated tracking system (San Diego Instruments, CA). On the first day, two identical objects (small cylindrical iron columns with a diameter of 5 cm and a height of 5 cm), termed as ‘old’, were placed 15 cm from the walls in the North–South orientation, and a mouse was placed at the mid-point of the wall opposite to the sample objects. After exploring the objects for 10 min, mice were put back in their home cage. 24 h later, one of the ‘old’ objects used for the memory acquisition was replaced with a ‘novel’ object (a small cylindrical iron cone with a diameter of 5 cm and a height of 5 cm) similar to the ‘old’ one. The mouse was again placed in the chamber for 10 min to explore the objects and the time spent exploring the novel and old object was assessed.

Y maze spontaneous alternation and spatial novelty preference test with three identical, opaque arms at 120° angles from each other, as previously described [12]. The arms were 35 cm long, 8 cm wide and 8 cm high, allowing the mice to see distal spatial cues. The continuous spontaneous alternation testing is based on the natural tendency of rodents to explore a novel environment. The mice were placed in the Y maze, facing the wall of one randomly chosen arm and could freely explore the three arms of the Y maze for 8 min. Typically, mice explore the least recently visited arm and tend to alternate between the three arms. For efficient alternation, mice rely on their spatial working memory [66]. Every arm entry was recorded by automated tracking system (San Diego Instruments, CA) in order to calculate the percentage of alternation. An entry was recorded when all four limbs were within the arm. The number of arm entries and total distance were used as measures for locomotor activity, while the spontaneous alternation percentage was used as a measure of spatial working memory. To calculate the percentage, the total number of alternations (i.e., every time a mouse explored the three arms consecutively) was divided by the total possible alternations (i.e., the total number of arm entries minus 2) and multiplied by 100.

In Y maze spatial novelty preference test, one of the three arms was closed and the mice could explore the other two arms for 10 min (training phase). Next, the mice were transferred to their holding cage for 2 h. Subsequently, they were placed into the start arm and could explore all three arms of the maze for 5 min (test phase). During these 5 min, the time and distance and number of entries in each arm were recorded by automatic tracking system (San Diego Instruments, CA) and those in the novel arm (i.e., previously closed during the training phase) were used as measures for spatial short-term memory.

The passive avoidance task was performed as previously described [27], with minor modifications. The apparatus consists of a two-chambered light–dark shuttle box (20.3 cm height × 21.3 cm width × 15.9 cm depth; ENV-010MC, Med Associates, USA) interconnected with a guillotine door. The experimental protocol consisted of two sessions; the training and the retention test session, conducted 24 h apart. During the training session, the animals were individually placed into the light compartment of the apparatus and explored the light compartment for a 30-s adaptation period with the door between the light and dark compartments closed. After the adaptation period, the door between the two compartments was automatically raised and mice were given an additional 5 min to explore the compartment during which the latency to enter the dark compartment was measured. Upon entering the dark compartment, the guillotine door was closed and 3 s later an inescapable foot-shock (0.5 mA, 2 s duration) was delivered through the grid floor. Thirty sec after the foot-shock, animals were immediately returned to their home cage. On the retention test day (24 h following training) mice were placed into the light compartment of the apparatus, and after a 30-s delay the door between the two compartments was raised. The latency to enter the dark compartment was automatically measured by the Instruments software. The trial was terminated after 5 min when the animal did not cross into the dark compartment.

Statistical analysis

Data are presented as the means ± SEM and were analyzed with GraphPad Prism software. Unpaired Student’s t test was used to determine the difference between two groups; one-way ANOVA analysis followed by Tukey’s multiple-comparison test (used for 1 variable with normal distribution sample) and two-way ANOVA analysis followed by Sidak’s multiple-comparison test (used for multiple variables) was used to determine differences between multiple groups, respectively. P < 0.05 was considered statistically significant. The details for the number of samples used can be found in Figure legends.

Data availability

The accession number for the RNA-seq data reported in this paper is GEO: GSE1693471.

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Acknowledgements

We thank for the technical support of the Core Facilities, Zhejiang University School of Medicine.

Funding

This work was supported in part by the National Natural Science Foundation of China (grants 92049108 to X.L., 91751325, 31270865 to W.Y.), the National Key Research and Development Program of China (2017YFE0196600 to X.L., 2016YFA0100303 to W.Y., 2017YFC1001703 to Q.S., 2019YFA0110103 and 2021YFA1101701 to B.S.), the Natural Science Foundation of Zhejiang Province (LZ19C090001 to B.S.). Q.S. was also supported by the Key Research and Development Program of Zhejiang Province (2017C03009).

Author information

** Peng
NHC and CAMS Key Laboratory of Medical Neurobiology, School of Brain Science and Brain Medicine, Zhejiang University, Hangzhou, 310058, Zhejiang, China
Binggui Sun
Zhejiang University Cancer Center, Zhejiang University, Hangzhou, 310029, China
Xuekun Li
Binjiang Institute of Zhejiang University, Hangzhou, 310053, China
Xuekun Li

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**aoxue Dong
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**yu Zhang
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Contributions

XL conceptualized the project. XD, JZ performed immunofluorescence staining, qRT-PCR, Western blot, immunoprecipitation and behavioral tests with the help of XY, LS and WQ analyzed RNA-seq data. XD, XZ, XC and YC conducted the astrocytes isolation and culture, maintained the animals and performed tamoxifen injection. XD and YS performed the ELISA assay. QZ and WY performed O-GlcNAcylation MS/MS analysis and the Click-it reaction assay. QL did the neuronal cell culture and analysis. XL, XD, LS, JZ, WQ, QS and WY analyzed the data. XL wrote the manuscript with the help from XD, JZ, GP, WY, QS and BS. All authors reviewed and approved the final manuscript.

Corresponding authors

Correspondence to Binggui Sun, Wen Yi, Qiang Shu or Xuekun Li.

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All experimental procedures were performed according to protocols approved by The Animal Ethics Committee of Zhejiang University. All experiments were conducted in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals (National Research Council, 1996).

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Supplementary Information

Additional file 1: Figure S1.

Ogt deficient astrocytes showed the reduced levels of Ogt and O-GlcNAcylation.Representative images of GFAP, Aldh1l1, Glast, Iba1, and Tuj1 immunostaining with cultured adult Ctrl astrocytes. Scale bar, 50 μm.Quantification results show that the percentage of GFAP⁺, Glast⁺ and Aldh1l1⁺ cells are all around 97%, the percentage of Tuj1⁺ cells is around 2%, and the percentage of Iba1⁺ cells is around 3%. n = 3 independent experiments. Values represent mean ± SEM.WB assayand quantification results showed that P1 and adult astrocytes showed the decreased levels of Ogtand Oga, but the increased Ogt/Ogaand O-GlcNAcylation levelcompared with cortical and hippocampal neurons. n = 3 independent experiments for each group. Values represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; one-way ANOVA analysis followed by Tukey’s multiple-comparison test, F = 38.5 for, F = 333.8 for, F = 51.81 for, F = 418.4 for. C: cortical neurons. H, hippocampal neurons; P1, postnatal day 1; Adult, postnatal 7-week.Schematic illustration of tamoxifen administration strategy. AdultGlast-CreERT2::Ogt^floxp/Y mice were intraperitoneallyadministrated with tamoxifenand corn oil, respectively. Eight weeks after tamoxifen administration, mice were sacrificed for assays.qRT-PCR results showed that mRNA level of Ogt significantly decreased in adult cKO astrocytes compared with Ctrl astrocytes. n = 3 independent experiments. Values represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; unpaired Student’s t-test.WB assayand quantification results showed that the protein levels of Ogtand O-GlcNAcylationsignificantly decreased in adult cKO astrocytes compared with Ctrl astrocytes. The level of Oga was also decreased in cKO cells. n = 3 independent experiments. Values represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; unpaired Student’s t-test.Representative images of GFAP-Ogtand Glast-O-GlcNAcylationimmunostaining with cultured adult Ctrl and cKO astrocytes. Scale bar, 50 μm.Representative images of GFAP-Ogtand Glast-O- GlcNAcylationimmunostaining with the brain sections of adult Ctrl and cKO mice. Scale bar, 20 μm.

Additional file 2: Figure S2.

Astrocytic Ogt deficiency does not affect microglia, and Ogt cKO in neural stem/progenitor cells does not induce inflammation in vivo.Three-dimensionalview of astrocytesand microgliaimmunostaining in the hippocampal regions of Ctrl and cKO mice. Scale bar, 20 μm.Quantification results showed that the level of Iba1 fluorescence intensityand the number of Iba1⁺ cellsshowed no difference in the hippocampus region between Ctrl and cKO mice. n = 4 mice per genotype. Values represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; unpaired Student’s t-test.Representative images of Iba1 immunostaining in the hippocampal regions of Ctrl and cKO mice. Scale bar, 20 μm.Sholl analysis showed no significant difference in the number of neurite intersections per radius, the number of neurites per cell, and the total length of neuritesof microglia in the hippocampal regions of Ogt cKO mice compared with Ctrl mice. 12 cells were picked up per animal and total 36 cells from 3 mice were analyzed per group; Values represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; two-way ANOVA analysis followed by Sidak's multiple-comparison test for, F = 5.062; unpaired Student’s t-test for.qRT-PCR results showed that mRNA level of Iba1 in the hippocampus showed no difference between Ctrl and cKO mice. n = 5 mice per genotype. Values represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; unpaired Student’s t-test.

Additional file 3: Figure S3.

Astrocytic Ogt deficiency impairs adult neurogenesis, and Ogt cKO in neural stem/progenitor cells does not induce inflammation in vivo.Representative images of DCX and BrdU immunostaining in the subgranular zoneof the hippocampus of Ctrl and Ogt cKO mice. Scale bar, 100 μm.Quantification results showed that cKO mice had the reduced number of BrdU⁺ cellsand DCX⁺cells, but a similar percentage of BrdU⁺DCX⁺/BrdU⁺in the SGZ of Ctrl and Ogt cKO mice. mice were injectedwith BrdU and sacrificed 1-day post the final BrdU administration. n = 4 mice per genotype. Values represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; unpaired Student’s t-test.Representative images of DCX and BrdU immunostaining in the subventricular zoneof the lateral ventricles of Ctrl and Ogt cKO mice. Scale bar, 100 μm.Quantification results showed that cKO mice had the reduced number of of BrdU⁺ cellsand DCX⁺cells, but a similar percentage of BrdU⁺DCX⁺/BrdU⁺in the SVZ of Ctrl and Ogt cKO mice. mice were injectedwith BrdU and sacrificed 1-day post the final BrdU administration. n = 4 mice per genotype. Values represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; unpaired Student’s t-test.WB assayand quantification resultsshowed that the protein level of Ogt and O-GlcNAcylation were not affected in proliferating adult neural stem/progenitor cellsof Ctrl and Ogt cKO mice. n = 4 independent experiments. Values represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; unpaired Student’s t-test.qRT-PCR results showed that under proliferating condition, Ctrl and cKO aNSPCs showed no difference in mRNA levels of Ogt, Sox2and Nestin. n = 4 independent experiments. Values represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; unpaired Student’s t-test.qRT-PCR results showed that under differentiation condition, Ctrl and cKO aNSPCs showed no difference in mRNA levels of mRNA level of Ogt, S100βand Map2. n = 4 independent experiments. Values represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; unpaired Student’s t-test.Representative images of GFAP and Iba1 immunostaining in the hippocampus of Ctrland NestinCreERT2::Ogt^loxp/Yadult mice. Scale bar, 100 μm.

Additional file 4: Figure S4.

Reactive astrocytes impair hippocampal neuronal cells and cognition of mice.ELISA results showed that the levels of IL-1βand TNF-αremarkably increased in the supernatants of cKO astrocyte-conditioned medium. n = 3 independent experiments. Values represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; unpaired Student’s t-test.Representative images of Tuj1 immunostaining with hippocampal neurons at day in vitro 10cultured with neurobasal medium, Ctrl and cKO astrocyte-conditioned medium, respectively. Scale bar, 20 μm.Sholl analysis showed that hippocampal neurons cultured with cKO ACM displayed the overall decrease in the number of dendritic intersections per radius, the number of dendrites per celland total length of dendritesat DIV 10 compared with NB- and Ctrl ACM-cultured cells. Values represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; n = 36 neurons from 3 independent experiments for each group; two-way ANOVA analysis followed by Sidak’s multiple-comparison test for, F = 353.5; one-way ANOVA analysis followed by Tukey’s multiple-comparison test for, F = 87.52 for, F = 168.7 for.Representative images of Tuj1 and activated-Caspase3immunostaining with hippocampal neuronscultured with neurobasal medium, Ctrl and cKO ACM, respectively. Scale bar, 50 μm.Quantification results showed that hippocampal neurons cultured with cKO ACM displayed the increased percentage of activated-Caspase3⁺ cells compared to that with NB- and Ctrl ACM-cultured cells. n = 6 independent experiments. Values represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; one-way ANOVA analysis followed by Tukey’s multiple-comparison test, F = 86.5.WB assayand quantification resultsshowed that the protein level of aCaspase3 significantly increased in hippocampal neurons cultured with cKO ACM compared to cell cultured with NB and Ctrl ACM, respectively. n = 3 independent experiments. Values represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; one-way ANOVA analysis followed by Tukey’s multiple-comparison test, F = 42.74.Schematic illustration of tamoxifen administration and behavioral tests strategy. adult Glast-CreERT2::Ogt^floxp/Ymice were intraperitoneally injected with tamoxifenand corn oil, respectively. Eight weeks after tamoxifen administration, behavioral tests were performed.Representative images of the swimming path of Ctrl and cKO mice during the probe trial in Morris Water Maze test. The red circle represents the position of platform.Adult Ctrl and cKO mice showed no difference for the length of swimming pathand average swimming speedduring the probe trial test. n = 10 mice per group. Values represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; unpaired Student’s t-test.Y maze spontaneous alternation test results showed no difference for the number of arm entriesand total distancebetween Ctrl and cKO mice. n = 8 mice per group. Values represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; unpaired Student’s t-test.

Additional file 5: Figure S5.

Restoration of O-GlcNAcylation inhibits astrocyte activation and inflammation, and improves cognitive function of Ogt deficient mice.Schematic illustration of tamoxifen and GlcNAc administration strategy. AdultGlast-CreERT2::Ogt^floxp/Y mice were intraperitoneally injected with tamoxifenand corn oil, respectively. 6 weeks after tamoxifen administration, Ctrl and cKO mice were injected with salineand GlcNAcfor 14 days, and then the mice were sacrificed for assays.Representative images of GFAP and IL-1β immunostaining with brain sections of Ctrl and cKO mice treated with salineand GlcNAc, respectively. Scale bar, 20 μm.Schematic illustration of tamoxifen, GlcNAc administration and behavioral test strategy. AdultGlast-CreERT2::Ogt^floxp/Y mice were intraperitoneally injected with tamoxifenand corn oilfor, respectively. Six weeks after tamoxifen administration, Ctrl and cKO mice were injected with salineor GlcNAcfor 14 days, respectively, and behavioral tests were performed. Saline and GlcNAc was continuously injected during the behavioral tests.Mice from the four group showed no difference in the percentage of time spent exploring two identical objectsand during the training period in novel object recognition task test. n = 6 mice per group; Values represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; two-way ANOVA analysis followed by Sidak's multiple-comparison test, F = 0.3952.Schematic illustration of novel object recognition task and heatmap image of each animal showing the distribution of exploring time of 4 groups mice during the testing trial. n = 6 mice per group.

Additional file 6: Figure S6.

Restoration of O-GlcNAcylation improves cognitive function.Mice showed no difference in the number of arm entries, total distancein Y maze spontaneous alternation test between four groups. n = 6 mice per group. Values represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; two-way ANOVA analysis followed by Sidak's multiple-comparison test for, F = 0.1623; one-way ANOVA analysis followed by Tukey’s multiple-comparison test for, F = 0.5806.Schematic Illustration of Y spontaneous alternation test and representative heat map images of mice during Y maze spontaneous alternation test.Representative images of the swimming path during the probe trial of Morris Water Maze test. The red circle represents the platform.The average swimming speedand total swimming path lengthshowed no difference in all four groups during the probe trial test. n = 6 mice per group; Values represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; one-way ANOVA analysis followed by Tukey’s multiple-comparison test, F = 0.3744 forand F = 0.3702 for.

Additional file 7: Figure S7.

Ogt interacts with NF-κB and catalyzes the O-GlcNAcylation of NF-κB.Schematic illustration of O-GlcNAcylation sites of p65 on S374, S370, S384 and S396 in N2a cells identified by MS/MS analysis. nanoLC-LTQ-CID was used to map** the sites of O-GlcNAcylation on p65. The matched fragment ions are labeled in y and b.IP-WB assayand quantification results show that mutation of single potential O-GlcNAcylation sites led to a significant decrease in the O-GlcNAcylation level of p65in N2a cells, and only the mutation of S384 induced a significant increase of p-p65. n = 3 independent experiments. Values represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; one-way ANOVA analysis followed by Tukey’s multiple-comparison test, F = 51.62 forand F = 74.06 for.IP followed by WB assay results showed the level of GSK3β showed no difference in the hippocampus of Ctrl and cKO mice, and the interaction between p65 and GSK3β was significantly increased in the hippocampus of Ctrl and cKO mice. n = 3 independent experiments. Values represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; unpaired Student’s t-test.co-IP followed by WB assay showed no direct interaction between Gsk3β and Ogt.

Additional file 8: Figure S8.

RNA-seq data analysis shows that Ogt deficiency activates NF-κB signaling pathway in astrocytes.Heatmap illustrating the altered transcriptome of cKO astrocytes group compared to Ctrl astrocytes. Red color, up-regulated genes; blue color, down-regulated genes. Two biological replicate samples were used for sequencing in each group.Gene ontologyCircle visualization of differentially expressed genesenriched biological process terms in cKO astrocytes including inflammatory response, cytokine signaling, NF-κB signaling, axon development and learning and memory. The outer circle showed the log2 fold changeof the genes in each category, and the height of the inner bar plot indicated the significance level of the GO term), and the color represents the Z score.WB assayand quantification results showed that Ogt deficiency did not affect the level of total p65, but significantly increased the protein level of p-p65in cultured astrocytes. n = 3 independent experiments. Values represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; unpaired Student’s t-test.WB assaysand quantification results showed that Ogt deficiency significantly reduced the level of p65 in cytoplasmand significantly increased the level of p-p65 in nucleus. n = 3 independent experiments. Values represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; unpaired Student’s t-test.

Additional file 9: Figure S9.

Restoration of O-GlcNAcylation inhibits astrocyte activation and inflammation, and improves cognitive function of Ogt deficient mice.Schematic illustration of GlcNAc administration strategy. 2.5-month-old Ctrl and AD mice were administrated with salineand GlcNAcfor 6 weeks, and mice were sacrificed for assays.Representative images of O-GlcNAcylation and Iba1 immunostaining with brain sections of Ctrl and cKO mice treated with salineand GlcNAc, respectively. Scale bar, 100 μm.Quantification results show that the level of of O-GlcNAcylation fluorescence intensitysignificantly decreased in the hippocampus region of AD mice compared to Ctrl mice, which was significantly increased by GlcNAc administration. The intensity of Iba1 fluorescence was also significantly reduced after GlcNAc administration. n = 5 mice per group. Values represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; one-way ANOVA analysis followed by Tukey’s multiple-comparison test, F = 10.64) for, F = 8.674.WB assaysand quantification results showed that the levels of O-GlcNAcylation, Ogt, Ogasignificantly decreased, but the level of APPsignificantly increased in AD astrocytes compared with Ctrl astrocytes. n = 4 independent experiments. Values represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; unpaired Student’s t-test.IP-WBshowed the reduced levels of O-GlcNAcylationand Ogt, but the levels of p65and GSK3βwere not affected in the hippocampus of Ctrl and AD mice. n = 4 mice per group. Values represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; unpaired Student’s t-test.Quantification results of IP inshowed that the decreased O-GlcNAcylation on p65, the decreased interaction between p65 and Ogt, and the increased interaction between p65 and GSK3βin the hippocampus of AD mice compared to Ctrl mcie. n = 4 mice per group. Values represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; unpaired Student’s t-test.

Additional file 10: Figure S10.

The original images of all western blot assays.

Additional file 11: Table S1.

The list of proteins interacted with p65 in Ctrl and cKO hippocampal tissues.

Additional file 12: Table S2.

The DEGs in cKO astrocyte.

Additional file 13: Table S3.

Reagents and experimental model resources.

Additional file 14: Table S4.

The list of primers used for qPCR, plasmids construction and and genoty**.

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Dong, X., Shu, L., Zhang, J. et al. Ogt-mediated O-GlcNAcylation inhibits astrocytes activation through modulating NF-κB signaling pathway. J Neuroinflammation 20, 146 (2023). https://doi.org/10.1186/s12974-023-02824-8

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Received: 29 November 2022
Accepted: 05 June 2023
Published: 22 June 2023
DOI: https://doi.org/10.1186/s12974-023-02824-8

Ogt-mediated O-GlcNAcylation inhibits astrocytes activation through modulating NF-κB signaling pathway

Abstract

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Introduction

Results

Ogt deficiency leads to the activation of astrocytes in vitro and in vivo

Activated astrocytes induce inflammation in Ogt deficient mice

Ogt deficiency in astrocytes impairs neurons and cognitive function of mice

Restoration of O-GlcNAcylation inhibits astrocyte reactivation and inflammation

Restoration of O-GlcNAcylation improves behavioral deficits of Ogt deficient mice

Ogt catalyzes the O-GlcNAcylation of NF-κB p65 and inhibits the activation of NF-κB signaling pathway

Ogt depletion induces inflammation by activating NF-κB signaling in human astrocytes

Restoration of O-GlcNAcylation inhibits inflammatory activation of AD astrocytes

Immunoprecipitation/co-immunoprecipitation (IP/co-IP)

Total RNA extraction and quantitative real-time PCR (qRT-PCR)

RNA-seq and data analysis

Gene ontology analysis

Behavioral tests

Statistical analysis

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