Introduction

Cyanobacteria as microbial cell factories possess high potential for sustainable production of various industrially relevant chemicals by direct carbon capture and conversion. High production of a target compound requires a significant redirection of metabolic flux for its synthesis, which might be challenging to achieve for the products of interest that are derived from secondary metabolism [22]. The resulting constructs were transformed into three Synechocystis strains: WT, AtC, TsC [17]. The AtC and TsC strains are used as control strains for production comparison. All created plasmids and strains are summarized in Table 1.

Interestingly, for some constructs, we were unable to obtain colonies after transformation. For instance, construct pPPD-BE–C, expressing tyrB from E. coli and Z. mobilis tyrC in an operon, was not possible to generate in the WT background, but for PAL and TAL expressing Synechocystis strains full segregation was successfully achieved. The construct pPPD-P–C-CM, generated to express PAA-AT, TyrC and CM from an operon, could not be transformed into any of the Synechocystis strains used, suggesting that the combined expression of the enzymes has a detrimental effect on the cell. Additionally, we noticed that for some constructs (pPPD-C, pPPD-CM) the full segregation was possibly combined with compensatory mutation(s), since the color of partially segregated colonies was changed from pale to healthy bright green when full segregation was achieved. In the cases of ΔppdC, ΔppdTsC, ΔppdE-C-CM and ΔppdTsE-C-CM completely segregated strains were not obtained.

Comparative production of tCA and pCou by engineered strains

Engineered Synechocystis strains were cultivated under constant light intensity for seven days and sampled on days four and seven for LC–MS analysis to detect Phe, Tyr, tCA and pCou in the growth medium. No Phe or Tyr was detected in the culture medium of strains obtained with the WT background. It appears that the knockout of ppd and introduction of the pathway genes alone or in any of the obtained combinations, were not sufficient to promote secretion of Phe or Tyr from the cell. The presence of a heterologous sink may be crucial for efficient consumption of the central metabolites Phe and Tyr.

Western blot analysis (Fig. 2) was performed on WT-based strains, since Western blots of extracts from the AtC and TsC strains exhibited extra bands of smaller molecular sizes [23], overlap** and interfering with detection of other strep-tagged proteins. Interestingly, the results showed that the protein level of TyrC when expressed alone in the not fully segregated strain ΔppdC is considerably lower than when expressed as a second gene in an operon. No expression of the plant PPA-AT was detected at tested conditions in strains ΔppdP and ΔppdP-CM, whereas in the ΔppdP-C strain, the PAA-AT was barely detectable while TyrC showed the thickest band. Plausibly, the activity of the upstream transaminase enzymes when combined with TyrC decreased the possible burden on the cell such as depletion of prephenate pool for phenylalanine synthesis caused by TyrC activity. The CM domain tagged with Strep-tag was not detectable by Western blot possibly due to its smaller molecular size, however in the screening tests in E. coli the corresponding band of ~ 12.5 kDa was present (data not shown).

Fig. 2
figure 2

Expression of proteins in WT-based engineered Synechocystis strains. Upper panel displays the Western Blot using anti-Strep antibody with a total duration of exposure of 600 s and 1000 s (inset darker areas, columns ΔppdP, ΔppdC, and top band in ΔppdP-C); bottom panel shows the SDS-PAGE with 9 μg of protein crude extract. PPA-AT corresponds to the band size of ~ 51 kDa, TyrBE ~ 43,5 kDa, TyrBN ~ 41,6 kDa and TyrC to 32,1 kDa respectively

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The specific production titer of accumulated tCA and pCou in the growth medium during photoautotrophic growth is presented in Fig. 3. The tCA production of the ΔppdAt strain was nearly 1.5 times higher than that of the parental strain AtC accumulating 138 ± 3.5 mg L−1 of tCA. The overexpression of transaminases individually as well as tyrC gene alone showed no improvement compared to ΔppdAt, suggesting that the ppd deletion was crucial for the titer increase. The growth of all AtC-based engineered strains was similar to the control strain AtC (Fig. 4A–C).

Fig. 3
figure 3

Specific production titer of tCA (A) and pCou (B) of Synechocystis strains during photoautotrophic growth. Values are the means of three biological replicates and two technical replicates, error bars represent the standard deviation. Statistically significant differences were examined by Student’s t-test and are represented by an asterisk (*P < 0.05)

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Fig. 4
figure 4

Growth of tCA (A, B, C) and pCou (D, E, F) producing Synechocystis strains during seven days of experiment. Results are the mean of three biological replicates, error bars represent standard deviation

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The productivity of pCou in most engineered strains expressing TsTAL together with the ppd deletion was similarly increased, whereas the titer of ΔppdTsC strain increased nearly 1.5 times compared to the control strain TsC, reaching 72.3 ± 10.3 mg L−1 after seven days, (Fig. 3B). Except for ΔppdTsBN-CM strain, there were no significant difference in pCou titer among the strains with ppd knocked out. The obtained results also demonstrate little increase of pCou specific production from day four to seven, suggesting that Tyr synthesis is less coupled to growth phase of the cells than Phe. In case of tCA, production normalized per OD750 at day seven doubled to that of day four in some strains. It is plausible, that at early growth stages Phe would be incorporated into biomass, whereas when cells divide less frequently, more Phe becomes available for secondary metabolism synthesis. The growth of the overexpression strains was slightly better than the corresponding control strain TsC (Fig. 4 D, E, F), with the exception of ΔppdTsC and ΔppdTsC-CM strains, likely due to detrimental effects of TyrC gene overexpression, which also prevented full segregation in these strains. As mentioned above, possibly, the expression of TyrC negatively affects the cell growth due to depletion of the prephenate pool for phenylalanine synthesis, whereas when combined with any of the aminotransferase enzymes, the negative effect is relieved.

Overexpression of the CM domain insensitive to Phe inhibition (ΔppdAtCM, Fig. 3A) led to a decrease of the tCA synthesis compared to both AtC and ΔppdAt strains, whereas the pCou titer in strain ΔppdTsCM (Fig. 3B) was at the same level as the ΔppdTs control. An increase of intracellular prephenate availability theoretically should enhance the flux of precursors to Phe and Tyr. The pCou production and consequently Tyr synthesis, was not affected by CM overexpression. Previously, it was suggested that the native prephenate dehydratase, encoded by pheA, is subject to feedback inhibition by Phe in the closely related strain Synechocystis sp. 29108 [2]. The overexpression of transaminases in an operon with CM restored the titer of tCA to control AtC strain levels (Fig. 3A, strains ΔppdAtBE-CM, ΔppdAtBN-CM and ΔppdAtP-CM). Since TyrB from E. coli has a broad aromatic substrate specificity, and although the substrate preference of TyrB from Nostoc is unknown, it is possible that the heterologous transaminase activity redirects the carbon flux into arogenate and Tyr (see Fig. 1) and thus relieves the inhibition of PheA by Phe. Possible regulation mechanisms involved in Tyr and Phe synthesis in Synechocystis are summarized in Fig. 5.

Fig. 5
figure 5

Enzyme regulation of Phe and Tyr biosynthesis possibly existing in Synechocystis. Summarized and adapted from references [1,2,3,4] and also based on results in this study. Abbreviations: CM chorismate synthase; DAHP, 3-deoxy-D-arabinoheptulosonate 7-phosphate; DAHPS, DAHP synthase; E4P, erythrose-4-phosphate; HGA homogentisate; HPC hydroxyphenyl acetate; HPP, 4-hyroxyphenylpyruvate; HPPD, 4-hydroxyphenylpyruvate dioxygenase; PAT prephenate aminotransferase PDT prephenate dehydratase; PEP, phosphoenolpyruvate; PPY, phenylpyruvate

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The overexpression of TyrC alone, which acts only on prephenate and arogenate as substrates resulted in similar tCA production titers as in the ΔppdAt strain, whereas the expression of TyrB transaminases in operon with TyrC decreased productivity to AtC strain levels (Fig. 3A), possibly because of a stronger partitioning of substrate towards Tyr. Integration of the TyrC overexpression cassette in WT and TsC never reached complete segregation. The double construct of tyrC and pheACM in an operon were not transformable in WT or AtC, suggesting that expression of these genes together is only viable when combined with TAL overexpression, although it did not enhance the pCou titer in ΔppdTsC-CM strain.

The intracellular tyrosine concentration might be the crucial key to this regulatory puzzle. The study by Hall and Jensen [2] suggested that Tyr acts as an activator of PheA (Fig. 5), and the activation effects of Tyr is as strong as the inhibiting effect of Phe. Possibly, the pattern of coupled Tyr and Phe biosynthesis, where enzyme activity of one branch can be affected by the product of another, ensures the required ratio of Phe/Tyr synthesis for the cellular needs [24]. Indeed, it has been shown previously that the addition of a degradation tag to PheA in an aromatic amino acid overproducer strain expressing feedback resistant aroG and TyrA from E. coli resulted not only in decrease of Phe, but also in Tyr [25]. In our case, tyrC overexpression in combination with pheACM and transaminases would enhance Tyr biosynthesis, leading to PheA activation, thus redirecting the flux away towards Phe synthesis and thereby no increase of pCou titer is observed. In case of the PAL overexpression strains, ΔppdAtE-C-CM and ΔppdAtN-C-CM had no improvement of tCA titer with respect to control AtC, whereas strains ΔppdAtE-C and ΔppdAtN-C produced less than the AtC control strain. In analogy with the above reasoning, tyrosine activation of the Phe branch would be caused by initial Tyr accumulation but will not result in a net accumulation of Phe, as the upstream pathway will then be downregulated to maintain a balanced Phe/Tyr ratio.

Similarly as observed previously [17, 26], the effect of the heterologous sink created by PAL overexpression results in slower growth, possibly due to decreased intracellular Phe concentration. This could further indicate that only the elevated Phe levels are controlling the flux into shikimate pathway via DAHP synthase inhibition, whereas the cell response to low Phe levels is slowing down the growth and biomass formation rather than compensatory pathway upregulation due to consumption of Phe by PAL. This is further supported by the observation that overexpression of feedback resistant DAHP synthase and TyrA from E.coli [25] led to Phe and Tyr accumulation in the media, but lower biomass accumulation, while improved growth rate was obtained when the natural mutagenesis was applied [26, 27].

The strong re-routing of Phe to tCA may have other detrimental effects on the cell, such as in response to high concentrations of tCA, or effects on regulation of other reactions and related metabolites. With regard to effect of high tCA concentration, in our previous work supplementation of AtC culture with Phe during photoautotrophic growth resulted in the Phe conversion into tCA with the tCA concentration above 0.5 g/L and the growth of the culture was not affected [26]. Nevertheless, since all the reactions involved Tyr and Phe metabolism in cyanobacteria are not characterized, potential effects are difficult to predict.

Undoubtedly, the regulation of Phe and Tyr synthesis is tight and complex, and their intracellular concentrations is probably maintained in a dynamic equilibrium. Metabolic flux analysis would be important to identify the partitioning of precursors into the Phe or Tyr biosynthetic branch. Jensen and Hall suggested that cyanobacterial control of the aromatic amino acids pathway is endo-oriented, where the pathway regulation is tuned to the endogenous formation of the initial pathway substrates, and thereby the earliest enzyme controlling the flow has the major role in the end-product regulation [24]. Thus, the loss of early-pathway regulation would provide sufficient elevation of substrate levels, and eliminate the flow restrictions by terminal branch enzymes leading to extracellular accumulation of Phe and Tyr as was observed previously [25]. Similarly, in our study, there was no Phe or Tyr secretion observed with overexpression of the terminal enzymatic steps in Phe and Tyr synthesis.

Comparative production of tCA and pCou by engineered strains in PRM8 background

As the next step, we aimed to combine obtained strains with the overexpression of a feedback resistant version of DAHP synthase, the first enzyme in the shikimate pathway, in order to relieve the early-flux controlling node. Yet, the transformation of the AroG150-pEERM3 construct into any of the engineered strains as well as Synechocystis WT was unsuccessful, consistently resulting in false-positive colonies after several transformation trials. An overexpression of feedback resistant aroG from E.coli in Synechocystis has been already demonstrated previously (aroGL175D [27], and in [25] with no mentioning of the mutation site). The mutation aroGL150P was described to result in an activated version of DAHP synthase [5], thus it might have placed some metabolic burden to the cell leading to an unsuccessful transformation in Synechocystis.

As an alternative approach, we used PRM8 (Phenylalanine Resistant Mutant #8), obtained from previous study [26], as a background strain for overexpression of created genetic constructs. This metabolic mutant of Synechocystis was shown to have a deregulated shikimate pathway with a mutation in a native DAHP synthase resulting in accumulation of high volumetric titers of phenylalanine when cultivated in high-density cultivation conditions. Thus, we have overexpressed several constructs, including the ppd knockout, in PRM8 based strains AtPRM8 and TsPRM8 harboring PAL and TAL activities respectively [26] (for simplicity these strains’ names have been shortened to At8 and Ts8 in this paper). Productivity of resulting engineered strains (Table 1) was compared to the corresponding ΔppdAt or ΔppdTs control strains. The results of the photoautotrophic growth after 7 days demonstrated that the productivity of tCA was not improved when At8 was used as a background strain (Fig. 6A). The growth of PRM8 based engineered strains was similar or slightly better compared to the control ΔppdAt strain (Fig. 7A). In case of pCou production, only the strain ΔppdTs8C reached specific productivity titer of the control strain ΔppdTs (Fig. 6B) although the growth of this strain was reduced nearly by half compared to the rest of the strains (Fig. 7B), while for the remaining strains the pCou titer was lower compared to the control strain. Notably, the growth of all engineered pCou-producing strains based on PRM8 was slower than of the ΔppdTs control, which is opposite for tCA-producing strains (Fig. 7). The ppd knockout seemed to cause a negative effect on growth of TsPRM8 strains in standard photoautotrophic conditions.

Fig. 6
figure 6

Specific production of tCA (A) and pCou (B) during photoautotrophic growth of Synechocystis PRM8-based strains. Values are the means of three biological replicates and two technical replicates, error bars represent the standard deviation. Statistically significant differences were examined by Student’s t-test and are represented by an asterisk (*P < 0.05)

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Fig. 7
figure 7

Growth of At8 (A) and Ts8 (B)-based engineered strains of Synechocystis during seven days of experiment. Results are the mean of three biological replicates, error bars represent standard deviation

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In the previous study [26], the tCA and pCou production during shake flask cultivation experiments was decreased in PRM based strains compared to WT based AtC and TsC control strains, while high-density cultivation conditions revealed a superior productivity phenotype of PRM8 based strains. Similarly, we have performed a high-density cultivation experiment to determine the effect of ppd knockout on selected strains in this specific growth condition. The results presented in Fig. 8A and B showed that although ppd knockout increased tCA and pCou production compared to AtC and TsC controls similarly to shake flasks experiment, when At8 was used as background, the tCA titer decreased with ppd knockout in strain ΔppdAt8. In case of pCou production (Fig. 8B), ΔppdTs8 strain reached higher specific titer than Ts8, however the productivity of Ts8 strain resulted to be lower than previously described [26].

Fig. 8
figure 8

Productivity and growth of PRM8-based strains during high-density cultivation. A Comparison of tCA production between engineered and control strains. B Comparison of pCou production between engineered and control strains. Samples were taken from the growth medium at day two and day four. The values are the means of three biological replicates and two technical replicates, error bars represent the standard deviation of three biological replicates. C Growth curves of tested strains during four days of cultivation. Error bars represent standard deviation of three biological replicates. Statistically significant differences were examined by Student’s t-test and are represented by an asterisk (*P < 0.05)

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Thus, the usage of PRM8 as a background strain did not enhance tCA and pCou titer even when combined with ppd knockout, as the improvement of productivity with deleted HPPD activity was only observed in WT based strains, which in summary demonstrates the complexity of shikimate pathway regulation.

Phenotypic effect of TAL overexpression

The expression of heterologous TAL in the WT or Δppd Synechocystis background was coinciding with the appearance of brown culture supernatant, which we have observed previously in experiments with TsC strain expressing TAL (K. Kukil, unpublished data) (Fig. 9). Similar evidence was reported for the overexpression of HPPD from A. thaliana in Synechocystis, and overexpression of α-ketoisocaproate dioxygenase from Rattus norvegicus, which possess the HPPD activity [10, 28]. The brown coloration is likely due to the formation of pyomelanin, a melanin-like pigment, a reaction product of HGA oxidation and polymerization [29]. Unlike in previous studies where the co-occurrence of the brown coloration reported is due to extra HPPD activity and thus increased HGA accumulation, in case of TsC strain, the TAL overexpression is supposed to increase the Tyr consumption by diverting it into the heterologous compound pCou. It is likely that the direction of Tyr into tocopherol biosynthesis involves certain regulation. In the above-mentioned study on overexpression of A. thaliana HPPD, when HPPD was coexpressed with geranylgeranyldiphosphate hydratase (GGH) from A. thaliana in Synechocystis, the supernatant did not turn brown, but accumulation of tocochromanols was increased [10]. The availability of prenyl chain precursors thus seems to be a bottleneck for efficient utilization of HGA into vitamin E synthesis. Synthesis of HGA from HPP is a multistep exergonic reaction involving oxidative decarboxylation, phenyl ring hydroxylation and side-chain rearrangement, and this reaction is thus irreversible [30]. Possibly, the TAL expression in Synechocystis alters the Tyr partitioning leading to increased HGA synthesis and brown coloration of the media. Since the substrate usage of Synechocystis HPPD enzyme is not known, there is a possibility that HPPD apart from HPP may also use PPY or other aromatic and non-aromatic keto-acids (Fig. 1).

Fig. 9
figure 9

Appearance of brown color in growth medium of Synechocystis. Left panel: Stationary culture (two weeks of cultivation). Culture medium of AtC strain is shown for comparison. Right panel: Agar plates after incubation for several weeks

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It is also puzzling that the brown color was present in the cultures of TsTAL expressing strains where ppd, encoding HPPD, was deleted (Fig. 5). Nowicka and Kruk observed the incorporation of radiolabeled HGA into the Tyr pool in Synechocystis, suggesting the existence of an unknown pathway for conversion of aromatic compounds [4]. Moreover, it was reported recently that the deletion of arogenate dehydrogenase, encoded by tyrA, from the terminal branch of Tyr biosynthesis is possible [3]. Therefore, either the report on the tyrA knock out strain is in conflict with the supposed essentiality of this gene in cyanobacteria [31] or it leads to the possibility of existence of another route to Tyr. It is tempting to suggest that the brown coloration in Δppd strains is a result of HGA accumulation via a not yet identified route of aromatic compounds metabolism.

Altogether, Synechocystis cells exhibit a strong response to the overexpression of heterologous genes from the shikimate pathway, leading to either absence of colonies after transformation, or likely accumulating some compensatory mutations to reach full segregation. This demonstrates the high rigidity of the cellular metabolism of aromatic compounds, which makes it a challenging target for metabolic engineering.

Conclusions

In this study following the aim to establish the cyanobacteria as microbial cell factories for sustainable production of phenylpropanoids, we engineered previously obtained Synechocystis strains AtC and TsC in order to enhance their tCA and pCou productivity, respectively. We demonstrate that inactivation of HPPD promoted the tCA and pCou synthesis nearly 1.5 times compared to parental strains, reaching 138 ± 3.5 mg L−1 of tCA and 72.3 ± 10.3 mg L−1 of pCou after seven days of photoautotrophic growth. However, there was no further improvement of productivity, when HPPD knock out was combined with heterologous overexpression of genes from the terminal steps of phenylalanine and tyrosine synthesis, possibly due to tight regulation of the phenylalanine and tyrosine biosynthetic pathway. In order to resolve this issue we used PRM8 based strains for overexpression of the designed synthetic constructs. However, the resulting tCA and pCou titers were not enhanced compared to WT background strains.

A comprehensive investigation of aromatic amino acid metabolism is needed, such as metabolic flux analysis in order to explore the possible existence of alternative routes leading to tyrosine formation as well as the possibility of decoupling the tyrosine synthesis from phenylalanine, which appears to be difficult in Synechocystis.

Methods

Bacterial strains and growth conditions

Escherichia coli DH5α-Z1 (Invitrogen) was used for subcloning and conjugation. E. coli cultures were cultivated in LB medium at 37 °C and supplemented with appropriate antibiotics to the final concentrations in the medium: 50 μg·ml−1 kanamycin (Km) or 200 μg·ml−1 erythromycin (Em) (Sigma, Merck).

In this study the model cyanobacterial strain Synechocystis sp. PCC 6803, a glucose-tolerant unicellular strain, was used. Cultures were grown in BG11 medium [32] with respective antibiotics Km 25 μg·ml−1 and/or Em 25 μg·ml−1 at 30 °C under constant 45 µmol photons m−2 s−1 light. The optical density of Synechocystis cultures was measured on Varian Cary 50 BIO spectrophotometer at a wavelength of 750 nm.

Plasmid construction for gene expression

In this study, all overexpressed genes were inserted in the ppd gene (slr0090) loci. For this, firstly, a ppd knock out vector, pPPD, was constructed based on the integrative vector pEERM3 [20]. Flanking regions of 1 kb length were amplified upstream and downstream of slr0090 from Synechocystis genomic DNA and cloned into pEERM3. Then, the antibiotic resistance cassette was changed from original chloramphenicol to Em using the PstI and SacI restriction sites. A sequence of a strong constitutive promotor Ptrc2O, synthetic ribosomal binding site RBS* [22], followed by XbaI restriction site, Strep-tag sequence with glycine-serine linker at the N-terminus position and BamHI and PstI cloning sites was amplified form pEEKN vector [17] and cloned using EcoRI and PstI restriction sites creating the pPPD vector (Fig. 10).

Fig. 10
figure 10

Schematic representation of vector construction. The base vector pPPD was cut with corresponding restriction site to insert ORF1 (single constructs). The addition of next ORFs was performed by cutting the new vector with BcuI and PstI (or BamHI) and the next insert with XbaI and PstI (or BamHI) and creation a BcuI/XbaI scar. DAHP synthase encoded by aroG was amplified from E. coli using E. coli cells as template. The gene was inserted into the integrative vector pEERM3. The expression was driven by a constitutive promotor Ptrc2O and synthetic ribosomal binding site RBS*, the gene sequence was followed by a flexible Gly-Ser linker and Strep-tag sequence at the C-terminal position. In order to create a feedback-resistant version of E.coli DAHP synthase, a Pro150Leu substitution [5] was introduced by mutagenesis PCR, creating the construct AroG150-pEERM3

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E. coli genes for overexpression were amplified using the E. coli BL21(DE3) genomic DNA as template. All other genes were codon optimized and synthesized by GenScript. Transaminase genes tyrB form Nostoc sp. 3335mG (GenBank PXA95964.1) and the CDS of prephenate aminotransferase from Arabidopsis thaliana (At2g22250/NP_565529.1) with the amino acid substitutions T84V and K169V were carrying the 3’ overhang of a BcuI restriction site, and were cloned into the pPPD vector using BamHI and PstI restriction sites. The tyrB gene from E. coli already contained a PstI restriction site, therefore during gene amplification a 5’ overhang with XbaI restriction site followed by Strep-tag sequence and a 3’ overhang with BcuI restriction site were introduced. Then the amplicon was cloned into the pPPD vector using XbaI and BamHI sites. Each transaminase expressing construct was combined in an operon with the tyrC gene (UniprotKB-Q04983), encoding cyclohexadienyl dehydrogenase from Zymomonas mobilis subsp. mobilis (strain ATCC 31821/ZM4/CP4) [33] and/or pheACM, encoding the chorismate mutase domain of the fused chorismate mutase/prephenate dehydratase from E.coli (UniProtKB- P0A9J8). The tyrC sequence fragment carried a 5’ overhang of XbaI site and RBS* and a 3’ overhang with a strep-tag sequence, BcuI and PstI (or BamHI in case of tyrB from E. coli) restriction sites. The fragment of 327 bp that corresponds to the chorismate mutase domain (residues 1 to 109) of CM/PDH from E. coli, in a same manner as tyrC carried a 5’ overhang of XbaI site and RBS* sequence and a 3’ overhang with a strep-tag sequence, and BcuI and PstI (or BamHI in case of tyrB from E. coli) restriction sites. Both fragments were cloned sequentially by cutting with XbaI and PstI, while the backbone was cut with BcuI and PstI respectively, thereby creating an ACTAGA scar from the BcuI/XbaI ligation. In addition, the tyrC and pheACM genes were cloned singly into pPPD vector as well as two together without an upstream transaminase gene.

Transformation of Synechocystis

For transformation, Synechocystis WT cells, PRM8 strain as well as engineered strains already possessing the Km resistance, were transformed with the series of pPPD expression vectors as described previously [22]. Colonies that appeared after 10–14 days were analyzed using PCR and restreaked on plates repeatedly or cultivated in liquid BG11 medium with appropriate antibiotics until full segregation was reached.

Western blotting

Soluble protein fraction of Synechocystis cells was extracted as described previously by Ivleva and Golden 2007 [34]. Proteins were separated by SDS-PAGE, using Mini-PROTEAN TGX™ gels (Bio-Rad), and transferred to a PVDF membrane (Bio-Rad) according to standard protocols. For detection of strep-tagged proteins Anti-Strep-tag II (Abcam) antibodies were used.

Determination of tCA and pCou by LC–MS

Determination of tCA and pCou in the growth media was performed by LC–MS. For this 1 ml of supernatant was taken from Synechocystis cultures at certain days of experiment, samples were filtered through 0.2 μm pore PTFE filters (Fisherbrand) and subjected to HPLC analysis. Samples were stored at − 20 °C if not analyzed the same day.

HPLC–MS analysis was performed using an Agilent 1290 Infinity II HPLC system equipped with a 1290 Infinity II High Speed pump and a 1260 II Infinity DAD HS UV–vis detector, using an InfinityLab POROSHELL SB-120 C18 column with dimensions of 100 mm × 2.1 mm and 2.7 μm particle size. The HPLC was coupled to an InfinityLab LC/MSD equipped with an ESI ionization source. LC separation was performed using a water (A, 0.1% formic acid) and acetonitrile (B) eluent system using the method: 0–1 min 10% B; 1–10 min 10 → 90% B; 10–11 min 90% B; 11–11.1 min 90–10% B, 11.1–12 min 10% B; at a flow rate of 0.3 ml/min. The quantification of tCA and pCou in Synechocystis cultures was based on a linear calibration curve from standards measured in technical triplicates. Standards for tCA and pCou (Sigma, Merk) were prepared in BG11 medium in the range 1–200 μg·ml−1 and filtered before analysis.