Abstract
The metabolism of Ibutamoren (MK-677), a nonpeptide growth hormone secretagogue, was investigated by analyzing human urine samples collected over 2 weeks following a single oral administration of the drug. Sample preparation involved dilution and enzymatic hydrolysis followed by a liquid–liquid extraction. Compounds were detected by reversed-phase ultra-high-performance liquid chromatography with quadrupole–time-of-flight mass spectrometric detection, using electrospray ionization in the positive ion mode. In the urine samples, the unchanged compound and its metabolites were identified. The hydroxylated analyte can be detected 4 days after a single oral administration of the compound, making it a promising target for detection.
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ACKNOWLEDGMENTS
The experiments were performed using scientific equipment of the Center for Collective Use “Ecological Analytical Center” of Kuban State University.
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This work was supported by the State Contract of the RF Ministry of Science and Higher Education, project no. FZEN-2023-0006.
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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. Informed consent was obtained from all individual participants involved in the study.
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Translated by O. Zhukova
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Dmitrieva, E.V., Temerdashev, A.Z., Gashimova, E.M. et al. A Study of the Metabolism of Ibutamoren (MK-677), a Growth Hormone Secretagogue, in Human Urine by Ultra-High-Performance Liquid Chromatography–High-Resolution Mass Spectrometry. J Anal Chem 79, 219–223 (2024). https://doi.org/10.1134/S1061934824020072
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DOI: https://doi.org/10.1134/S1061934824020072