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Determination of Andarine (S-4), a Selective Androgen Receptor Modulator, and Ibutamoren (MK-677), a Nonpeptide Growth Hormone Secretagogue, in Urine by Ultra-High Performance Liquid Chromatography with Tandem Mass-Spectrometric Detection

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Abstract

A procedure for the determination of a selective androgen receptor modulator andarine (S-4) and a nonpeptide growth hormone secretagogue ibutamoren (MK-677) in urine has been developed including sample preparation by the “dilute-and-shoot” procedure, separation of analytes by ultra-high performance liquid chromatography in the gradient elution mode, and mass-spectrometric detection with heated electrospray ionization. The limits of detection are in the range of 0.5–2.5 ng/mL, the calibration curves are linear in the range of 2.5–250 ng/mL for andarine and 5–250 ng/mL for ibutamoren. The proposed procedure was used for the analysis of urine samples obtained from volunteers after a single administration of these drugs containing 15 mg of active substances.

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Authors and Affiliations

  1. Kuban State University, Krasnodar, 350040, Russia

    E. V. Dmitrieva, A. Z. Temerdashev, A. A. Azaryan & E. M. Gashimova

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  1. E. V. Dmitrieva
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  2. A. Z. Temerdashev
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  3. A. A. Azaryan
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  4. E. M. Gashimova
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Correspondence to E. V. Dmitrieva.

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Original Russian Text © E.V. Dmitrieva, A.Z. Temerdashev, A.A. Azaryan, E.M. Gashimova, 2018, published in Zhurnal Analiticheskoi Khimii, 2018, Vol. 73, No. 7, pp. 523–528.

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Dmitrieva, E.V., Temerdashev, A.Z., Azaryan, A.A. et al. Determination of Andarine (S-4), a Selective Androgen Receptor Modulator, and Ibutamoren (MK-677), a Nonpeptide Growth Hormone Secretagogue, in Urine by Ultra-High Performance Liquid Chromatography with Tandem Mass-Spectrometric Detection. J Anal Chem 73, 674–678 (2018). https://doi.org/10.1134/S1061934818070031

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  • DOI: https://doi.org/10.1134/S1061934818070031

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