Extended Data Fig. 8: Colonic EEC deficiency is associated with dysbiosis.
From: Interaction between the gut microbiota and colonic enteroendocrine cells regulates host metabolism
(a) Relative frequency of bacterial phyla as determined by 16 s sequencing from co-housed and genotype segregated WT and EECΔCol animals (n = 5 mice). (b) Volcano plots of differential abundance testing of fecal microbiota species (sequence variants) from WT and EECΔCol male mice at 4 (top, n = 8 WT or 5 EECΔCol mice) and 6–8 (bottom, n = 14 mice) weeks of age. Only statistically significant features are shaded in color. (c) Alpha diversity by feature abundance, ß-diversity PCoA by unweighed UNIFRAC (PERMANOVA p = 0.004), and volcano plots of differential abundance testing of sequence variants from iEECΔCol or tamoxifen (TMX) treated non-Cre control mice (13 weeks of age, n = 6 mice). (d) Representative microphotographs of HE-stained liver (left panels) and adipose tissue (right panels) of male EECΔCol mice under vehicle or antibiotic treatment, scale bar 100 µm (one representative animal in each group, n > 3 per group). (e) PCoA plots of ß-diversity analysis of FMT from donor (WT or iEECΔCol) and GF WT recipient mice (n = 5 mice). Respective PERMANOVA calculated p values are noted in the panels.