Abstract
ATP and its ionotropic P2X receptors are components of the most ancient signaling system. However, little is known about the distribution and function of purinergic transmission in invertebrates. Here, we cloned, expressed, and pharmacologically characterized the P2X receptors in the sea slug Aplysia californica—a prominent neuroscience model. AcP2X receptors were successfully expressed in Xenopus oocytes and displayed activation by ATP with two-phased kinetics and Na+-dependence. Pharmacologically, they were different from other P2X receptors. The ATP analog, Bz-ATP, was a less effective agonist than ATP, and PPADS was a more potent inhibitor of the AcP2X receptors than the suramin. AcP2X were uniquely expressed within the cerebral F-cluster, the multifunctional integrative neurosecretory center. AcP2X receptors were also detected in the chemosensory structures and the early cleavage stages. Therefore, in molluscs, rapid ATP-dependent signaling can be implicated both in development and diverse homeostatic functions. Furthermore, this study illuminates novel cellular and systemic features of P2X-type ligand-gated ion channels for deciphering the evolution of neurotransmitters.
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Introduction
In addition to being the critical energy storage for every cell, ATP acts as one of the most ancient intracellular and intercellular signal molecules1,2,3. The possible involvement of ATP in signaling mechanisms was initiated in the 1920s by Drury and Szent-Gyorgyi4; and then in the 1950s by Holtons5,6,7, leading to the concept of purinergic transmission in the 1970s by Burnstock3,8. Eventually, in 1983, rapid ATP-gated ion currents were discovered in neurons9,10 and muscles11, and specific subtypes of the ligand-gated P2X receptors were identified in the 1990s12,13,14,15. Finally, the 3D structure of P2X receptors was revealed in 2009–201216,17. These are distinctive trimeric ligand-gated channels showing a common architecture with acid-sensing ion channels but unrelated in their respective amino acid sequences18.
Comparative studies established that P2X-type receptors are broadly distributed across many eukaryotic lineages1,19,20,21, including most Metazoa2,3. Across all domains of life, ATP can operate as the bona fide ancient signal molecule (and a volume transmitter) associated with adaptive reactions to injury and damage2,3,22.
In selected evolutionary lineages, P2X receptors were secondarily lost. The list includes higher plants3, some bilaterians such as selected acoels22, arthropods, and nematodes. For example, Drosophila and C. elegans genomes have no P2X receptors, but other ecdysozoans such as Daphnia, the shrimp Litopenaeus, the tick Boophilus23, and tardigrades24 contain one receptor. Lophotrochosoans or Spiralia, including flatworms, also have one type of P2X receptor with shared pharmacological properties to mammals25. However, practically nothing is known about the functional roles of P2X receptors in the CNS and peripheral tissues of invertebrates and molluscs, in particular3. Mollusca is one of the most diverse animal phyla in terms of its morphological and biochemical adaptations.
The release of ATP from the central ganglia of the pond snail, Lymnaea stagnalis, was demonstrated26. Subsequently, P2X receptors were identified in this species with widespread expression across the CNS27 but unknown function(s).
Here, we show that ATP and its ligand-gated P2X receptors are components within chemosensory structures and the unique integrative neurosecretory center present in the CNS of the sea slug Aplysia—an important model for neuroscience28,29. Expression and pharmacology of P2X receptors in Aplysia confirms the preservation of evolutionary conserved bioenergetic reporter-sensor systems across animals and provides new tools to decipher homeostatic mechanisms in neuroendocrine systems and development.
Results
Identity, phylogeny, tissue-specific expression, and quantification of Aplysia P2X (AcP2X) receptors
We identified and cloned a single Aplysia P2X receptor with two splice forms (GenBank#: NP_001191558.1, NP_001191559.1), which shared 92% identity. The predicted structure of the Aplysia P2X receptor reveals all major evolutionary conservative sites and posttranslational modifications (Supporting Information, Fig. S1), which are similar to its homolog in another gastropod, Lymnaea27. The genomic organization of the P2X receptors confirmed the overall evolutionary conservation of exons and intron–exon boundaries. The Aplysia P2X receptor is similar to a relatively compact vertebrate P2X4 receptor with similar numbers and length in exons, but this is not always found in other invertebrates (e.g., Daphnia pulex and Nematostella vectensis have 8 exons each). However, the overall similarity of the P2X4 receptor and the molluscan receptor intron/exon boundaries suggests that this receptor structure was present before divergence chordates and molluscs.
Figure 1A shows the phylogenetic relationships among P2X receptors with distinct events of gene duplications in the lineages leading to humans, zebrafishes, hemichordates, echinoderms, and basally-branched metazoans such as sponges, placozoans, and cnidarians (see Supporting Information, Table S1 for sequences used in this analysis). In contrast, representatives of molluscs (including Aplysia), annelids, and parasitic flatworms (Schistosoma) appear to have a single copy of a P2X-encoded gene that often form distinct phyletic clusters within a respective phylum. This reconstruction suggests a single P2X gene in the common metazoan ancestor with independent multiplication events in selected animal lineages. It primarily occurred within vertebrates, as reflected in the whole-genome duplication events early in the evolution of this group. Interestingly, some bilaterians such as the acoel Hofstenia miamia, insects, and nematodes12 secondarily lost the P2X receptors. This mosaic-type phyletic distribution likely illustrates different system constraints for recruiting P2X receptors to novel functions or preserving ancestral molecular mechanisms of purinergic signaling.
Next, we characterized the expression of the P2X receptors in Aplysia using a broad spectrum of RNA-seq data obtained from adult and developmental stages30 (see Supporting Information, Table S2 for project SRA accession numbers). The highest level of AcP2X expression was found in the chemosensory structures (the mouth area and rhinophores31) and the gill (Fig. 1B), which is also known as the chemosensory and respiratory organ. Expression of AcP2X receptors was detected in the majority of the peripheral organs in Aplysia as well as during the first developmental stages (Fig. 1B), where no neurons or specialized sensory cells exist. Thus, ATP could act as a paracrine messenger in early embryogenesis.
The freshwater pond snail Lymnaea stagnalis is the only know molluscan species with the biophysical characterization of its P2X receptors27. The Aplysia P2X receptor's structural organization was comparable to Lymnaea’s P2X (Figs. 1C, 2; and Fig. S2 Supplementary Information) but with noticeable differences in their predicted ATP binding and other regulatory sites suggesting potentially different biophysical properties. These differences are also evident in the 3D models for related molluscan species (Fig. 2 and Fig. S2, Supplementary Information). Specifically, the differences between Aplysia and Lymnaea were found in the region of the P2X receptors known as the ‘dorsal fin’16,32. The crystallography analysis16 indicated that ATP binding causes rearrangement of the ‘head’, ‘right flipper’, and ‘dorsal fin’ domains (Fig. 2A,B). These conformational changes occur both around the ligand (ATP) binding site and between subunits16,33. Thus, the observed variability of amino acid sequences in these regions between Aplysia and Lymnaea suggests that marine and freshwater organisms are distinct in the kinetic and pharmacological properties of their respective P2X receptors. This possibility was experimentally tested, as we reported below.
Expression of AcP2X in Xenopus oocyte confirms the evolutionary conservation of kinetic and pharmacological parameters
ATP elicited an inward current in a concentration-dependent manner in oocytes injected with AcP2X (Fig. 3A). EC50s (concentrations of a drug that gives half-maximal response) were determined for both the fast- (0-1seconds) and the slow component of current with continuous application of ATP. The EC50 for the fast component was 306.0 µM with a 1.58 Hill coefficient, and for the slow component, 497.4 µM with a 0.97 Hill coefficient (n = 7, oocytes, Fig. 3B). The second application of the agonist, with a recovery time of 6 min, generated a 15–30% reduction in peak amplitude and is indicative of the rundown observed in other P2X receptor subunits. The response to 250 µM ATP produced a mean peak amplitude of 31.3 nA ± 3.8 nA and a time to a peak value of 2.76 ± 0.21 s (n = 19) with a holding membrane potential of − 70 mV (Fig. 3C). The ATP analog, 2′,3′-O-(4-Benzoylbenzoyl) adenosine 5′-triphosphate (Bz-ATP34) gave a partial response at 20% of the ATP response (n = 8, oocytes, Fig. 3C). There were no UTP and ADP responses within the same range of concentrations (data not shown).
The current–voltage relationship was investigated in the presence of elevated (144 mM) and low extracellular NaCl (96 mM) concentrations (n = 6, oocytes, Fig. 3D). A reversal potential was determined by applying a ramp protocol from − 70 to 20 mV in high and normal Na+ with 250 µM of ATP (Fig. 3E). According to the Nernst equation, the reversal potential was 13.9 mV and shifted by + 10.2 ± 1.3 mV to positive holding in high sodium solution (n = 6, oocytes).
P2X antagonist suramin34 inhibited ATP responses in a concentration-dependent manner (Fig. 4A,B; n = 7, oocytes). Another P2X antagonist, pyridoxal-phosphate-6-azophenyl-2′,4-disulfonic acid (PPADS34), also inhibited the response of ATP on AcP2X in a concentration-dependent manner (Fig. 4C,D). However, the application of PPADS produced a greater block than the suramin (Fig. 4E). Mean current responses to 250 µM ATP in the range of 1–250 µM PPADS generated an IC50 = 211.2 µM for the fast component, but the slow component could not be calculated (n = 7, oocytes, Fig. 4D). The second splice form of AcP2Xbwas also expressed in oocytes producing currents very similar to the first isoform AcP2X described above; however, it resulted in much smaller (and unstable) responses (data not shown).
P2X receptors are expressed in unique populations of neurosecretory cells of Aplysia
Interestingly, the CNS has the overall lowest P2X gene expression (Fig. 1B). This situation might be analogous to the recruitment of purinergic signaling in the chemosensation within the mammalian brain35, suggesting a distinct and relatively small population of ATP-sensing cells. We tested this hypothesis.
AcP2X was explicitly expressed in two symmetrical subpopulations localized in the F-cluster of the cerebral ganglion of Aplysia36,37. Each subpopulation contained about 25–40 cells (30–60 μm diameter, Fig. 5A,B)36. As illustrated in Fig. 5A, but not as in the present version Fig. 1A the labeled cells are not homogenous and might represent different subpopulations (larger and middle-size cells neurons36, and some of these neurons can be insulin-containing37). Application of 2 mM ATP to these neurons elicited a 2–5 mV depolarization (3.1 mV ± 2.2 mV n = 11), action potentials, and these effects were reversible (Fig. 5C) and voltage-dependent (Fig. 5D), consistent with the pharmacological properties of Aplysia’s P2X receptors expressed in oocytes. Neurons from neighboring cerebral clusters that were negative for AcP2X by in situ hybridization showed no response to as high as 10 mM ATP concentration (n = 31). These tests confirmed that P2X receptors in F-cluster neurosecretory cells are functional.
Discussion
As the central bioenergetic currency, the intracellular concentrations of ATP reach 1–10 mM with multiple mechanisms of its extracellular release across all domains of life2. In the molluscan CNS, the baseline level of ATP release can be increased following depolarization and serotonin application, suggesting that ATP can act as an endogenous fast neurotransmitter26. The presence of ionotropic P2X ATP-gated cationic channels in peripheral chemosensory structures (together with profound serotonergic innervations of these structures in molluscs38,39,40,41,42,43,44) and the CNS (Fig. 1B) further support this hypothesis. However, we also reported AcP2X receptor expressions early in development, suggesting that ATP might be a paracrine signal molecule controlling cleavage and differentiation.
The purinergic sensory transmission is widespread in mammals35 and might have deep evolutionary roots2,3,45. Mammalian P2X receptors13,46 are comparable to their homologs in Aplysia based on their sequence and kinetic parameters but have a lower sensitivity to ATP. As a part of future directions in comparative biophysical studies, it would be important to perform targeted mutagenesis of the molluscan P2X receptors to precisely identify amino acids responsible for the observed differences among mammalian and invertebrate P2X receptors.
The overall kinetic and pharmacological parameters of Aplysia P2X receptors are also similar to those described both in the closely related Lymnaea27 and distantly related Schistosoma25. However, the Lymnaea P2X receptor showed much higher sensitivity (EC50 is in µmolar range) to ATP than Aplysia, consistent with predicted structural differences of P2X receptors across species (Fig. 2). Suramin and PPADS both inhibited the ATP evoked responses in other species23,24,25,27, but in Aplysia, it occurred in a narrower range (10–250 µM) than in Lymnaea (0.1–250 µM). Suramin and PPADS are known as pan-P2X blockers. Thus, it might be expected that novel and, perhaps, more selective antagonists could exist for the Aplysia receptor subtype.
Ecdysozoan P2X receptors are also relatively diverse (although detailed mutagenesis studies have not been performed in any representatives of this largest metazoan lineage). In contrast to Aplysia, the tick P2X receptor displayed a very slow current kinetics and little desensitization during ATP application23. The tardigrade P2X receptor24 had a relatively low sensitivity for ATP (EC50 ~ 44.5 μM), but fast activation and desensitization kinetics—similar to Aplysia.
Aplysia P2X receptors exhibit a distinct phenotype, having a moderate ATP sensitivity (compared to the freshwater Lymnaea and mammals) but faster kinetics than some ecdysozoans. These “hybrid features” might be related to the marine ecology of Aplysia with a broader range of environmental changes. The agonist potencies (ATP and BzATP) in the cloned Aplysia P2X do not match any known P2X receptor subtype. It suggests that there was a specific diversification of P2X receptors in the lineage leading to Aplysia.
The currents recorded from the Aplysia F-cluster neurons do not fully resemble the currents recorded from the oocytes. They do not desensitize and get bigger as the membrane potential hyperpolarizes. These observations also suggest that either expression in Xenopus (as the freshwater animal with different osmolarity) modify the properties of native channels or implicate additional regulatory mechanisms for the Aplysia P2X receptors in the intact CNS. Additional integrative analyses would be needed to biophysically determine the channel properties and physiological role of AcP2X receptors.
Interestingly, the abundance of P2X receptors in the Aplysia chemosensory systems (such as mouth areas, rhinophores, and gills) correlates with the expression of nitric oxide synthase47, suggesting interactions of these afferent pathways in the control of feeding and respiration. Aplysia might also detect environmental ATP from bacterial and algal (food) sources2 as in some other studied marine species, including lobsters48.
The P2X receptor is one of many different receptors in the purinergic signaling complex. The ‘purinome’ includes P2 receptors, both ionotropic (P2X) and metabotropic (P2Y), P1 adenosine receptors, adenosine transporters ectonucleotidases49,50. Of note, the Aplysia genome has no P2Y receptors51. Still, it has twenty-four P1 adenosine receptors that are also putative purinergic G protein-coupled receptors, compared to the human genome that contains eight P2Y receptors and four P1 adenosine receptors51. Besides the purinergic receptors, there are two gene families related to purinergic signaling: adenosine transporter proteins, equilibrative (ENT) and concentrative nucleoside transporters (CNT)52,53,54. The Aplysia genome has no CNTs but contains five predicted ENTs, whereas the human genome has three CNTs and four ENTs. A putative vesicular nucleotide transporter, VNUT (SLC17a)55,56 was detected in the Aplysia genome (Solute carrier family 17 member 9, e-value = 0)57. However, it should be noted this predicted Aplysia gene (XP_005101701.3) is a tentative annotation without experimental validation of its specific nucleotide affinities (e.g., ATP). Its ortholog, VNUT, has only been functionally characterized in humans and mice55. XP_005101701.3 would be an essential gene for future experimental analysis of expression and selectivity determination in Aplysia.
The Aplysia genome has a representative complement of ectoenzymes (cell surface enzymes that can hydrolyze nucleosides and nucleotides extracellularly58,59), including the enzymes of the nucleoside triphosphate diphosphohydrolase (ENTPDase) family, the nucleotide pyrophosphatase/phosphodiesterase (ENPP) family, ecto-5′-nucleotidase (5NTD), alkaline phosphatase (AP), adenosine deaminase (ADA) and purine nucleoside phosphorylase (PNP)59. The presence of this type of extracellular enzymes can restrict the volume type transmission mediated by ATP, and purinergic signaling in the CNS might be more localized in 3D space and time.
The purinergic signaling system is prominent and widespread in the human brain. Yet, in the CNS of Aplysia, the detectable expression of the ionotropic P2X receptor is apparently confined to the F-cluster, emphasizing the integrative role of this neurosecretory center. F-cluster also has insulin-containing neurons36,37, likely associated with the systemic control of growth and, subsequently, reproduction. The release of the Aplysia insulin can decrease the level of glucose in the hemolymph37. Moreover, some F-cluster neurosecretory cells are electrically coupled36, which help synchronize their discharges and, eventually, insulin secretion. It is known that ATP can also be released from gap junctions (innexins) and during synaptic exocytosis2. Thus, we can propose that P2X-induced neuronal depolarization of insulin-containing neurons provides positive purinergic feedback, sustaining this multifunctional integrative center's excitability and secretory activity in Aplysia-related gastropods.
The potential functional roles of many differentially expressed genes in the F-cluster can be determined in the future. Their involvements in control of multiple behaviors, immunity is evidenced by a broad array of respective genes uniquely or differentially expressed in F-cluster (our recent RNA-sed data revealed unexpected diversity of specific transcripts controlling pain, injury, toxins, immunity [interleukins], feeding, and energetics [insulins], reproduction and potentially some novel functions [ecdysone-type receptors], etc.—LLM, ABK, unpublished data). This is the reason to view this cluster as one of the top-level integrative centers in the animal with the broadest spectrum of secretory peptides and multiple receptors, including P2X receptors.
This study also clearly illustrates that genomic/molecular predictions have to be experimentally validated as we performed here with the Aplysia P2X receptors. Indeed, many observed differences in kinetics and pharmacological properties of these Aplysia receptors (compared to other species) cannot be deduced from the sequence information. It further stresses physiological experiments' value in analyzing predictions of emerging massive comparative genomics/transcriptomics datasets.
Materials and methods
Animals and molecular analyses of Aplysia AcP2X
Aplysia californica (60–100 g) were obtained from the National Resource for Aplysia at the University of Miami. Animals were anesthetized by injection of 60% (volume/body weight) isotonic MgCl2 (337 mM) prior to the removal of the CNS. The ganglia were then pinned to a sylgard dish in artificial seawater (ASW: 460 mM NaCl, 10 mM KCl, 55 mM MgCl2, 11 mM CaCl2, 10 mM HEPES, pH = 7.6), and the cells were exposed by the mechanical removal of the overlying sheath with fine forceps.
Cloning
The original sequences were generated using RNA-seq profiling60,61,62,63. Details for RNA extraction and cDNA library construction have been described60,61,62,64.
Both 5′ and 3′ RACE were performed to obtain the full-length coding sequence. Full-length CDS sequence for AcP2X (GenBank# NP_001191558.1) was obtained using terminal primers: 5′-ATGGCTCCACCACAAGTCATGAAG-3′ and 5′-AAGCATCAAGGTGCGGCTCCTCCATCAC-3′. Amplified PCR product was cloned into pCR4-TOPO (Cat#K4575-01, LifeTechnologies). Four clones were isolated and sequenced together with a splice variant, AcP2Xb (GenBank# NP_001191559.1), and the full-length CDS for this isoform was also cloned and sequenced. Since there appears to be only one P2X gene in the Aplysia genome (GCF_000002075.1), we will designate the predicted protein as AcP2X.
In situ hybridization
The original sequences were obtained from Aplysia RNA-seq profiling60,61,62,63. We used the same protocols for whole-mount in situ hybridization as reported elsewhere65,66 with a specific probe for the validated AcP2X. The two isoforms of AcP2X vary by a 147 base deletion/insertion and would be difficult to distinguishable by in situ. The antisense probe was generated by digestion of the AcP2X plasmid with Not I (Cat#R0189s, New England Biolabs Inc.) then transcribed with T3 polymerase from the DIG RNA Labeling Kit (Cat#11175025910, Roche Diagnostics). The control sense probe was produced by the same protocol but used Pme1 (Cat# R0560s, New England Biolabs Inc.) for digestion and T7 polymerase for transcription.
Expression of AcP2X was investigated in central ganglia of 8 experimental and 2 control CNS preparations; additional controls were reported elsewhere60,66. Control in situ hybridization experiments with full length ‘sense’ probes revealed no specific or selective localization in the CNS under identical conditions and labeling protocols. Images were acquired with a Nikon Coolpix 4500 digital camera mounted on an upright Nikon Optiphot-2 microscope.
RNA-seq and quantification
The isolation of specific cells, RNA extraction, and preparation of cDNA for RNA-seq analysis as well as transcriptome annotation was performed using the same methods as reported elsewhere60,61,62,64.
Expression levels of transcripts were calculated using the normalization method for RNA-seq—Transcripts Per Million (TPM)67. Map** was performed in the STAR (2.3.0)/feature Counts analysis with the values obtained from the Bowtie2/Tophat pipeline68. The mapped reads were summarized and counted within the R statistical programming language. Supplementary Table S2 contains a list of RNA-seq projects and their corresponding SRA accession number.
Sequence analysis and phylogeny
Sequences were obtained through BLAST search across both Metazoans and non-metazoan groups and aligned using the MUSCLE or ClustalX ver. 2.1 programs with default parameters. Protein domains and motifs were obtained from Prosite49 and SMART50 databases. A maximum likelihood (ML) tree with the best-fit model (LG + G) was constructed using MEGA X51 and 10,000 iterations.
Protein modeling
The reconstruction of 3D-structures of the P2X receptor from Aplysia californica (NP_001191558.1, GenBank, NCBI) and Lymnaea stagnalis (AFV69113.1, Genbank, NCBI) was based on pdb ID: 5svk (open state) and 4dwo (closed state) modeling69. Alternative models of the same P2X receptors were generated using PyMol (The PyMol Molecular Graphics System, Version 1.8.6.0 Schrödinger, LLC) and Phyre2 software69,70,71,72.
Electrophysiology
Oocytes recordings
RNA preparation for oocyte injections and oocyte maintenance are described above. The oocyte recording bath was in ND96 medium (96 mM NaCl, 2 mM KCl, 1 mM MgCl2, 1.8 mM CaCl2, and 5 mM HEPES, pH = 7.4), or with the 1.8 mM CaCl2 being replaced by 1.8 mM BaCl2. Ba2+ was used in oocytes experiments only to block the endogenous chloride and potassium currents in Xenopus oocytes. Whole-oocyte currents were recorded by two-electrode voltage clamp (GeneClamp500B, Axon Instruments, Foster City, CA, USA) using microelectrodes made of borosilicate glass (WPI, USA) with a resistance of 0.5–1 MΩ when filled with 2.5 M KCl. Currents were filtered at 2 kHz and digitally sampled at 5 kHz with a Digidata 1320B Interface (Axon Instruments, CA). Recording and data analysis were performed using pCLAMP software version 8.2 (Axon Instruments). For data acquisition and clamp protocols, the amplifiers were connected via a Digidata 1320B AD/DA converter (Axon, USA) to an AMD PC with pClamp 8.2 voltage-clamp software (Axon, USA). Unfiltered signals were sampled at 10 kHz and stored digitally.
Data are presented as mean ± S.E. using Student’s paired t-test. Concentration–response data were fitted to the equation I = Imax/[1 + (EC50/L)nH], where I is the actual current for a ligand concentration (L), nH is the Hill coefficient, Imax is the maximal current, and EC50 is the concentration of agonist evoking 50% the maximum response. Respectively, the IC50 is the concentration of an inhibitor where the response (or binding) is reduced by half.
To compute the reversal potential for sodium, the Nernst equation used; Vj = (RT)/(zF)ln(c1/c2) where R is the gas constant 1.98 cal K−1 mol−1, F is the Faraday constant 96,840 C/mol, T is the temperature in oK and z is the valence of the ion.
In situ recordings
Voltage- and current-clamp experiments were carried out on identified F-cluster neurons in intact nervous systems of Aplysia36. ~ 0.5 mL bath was perfused with solutions (artificial sea water) using a gravity-feed system and a peristaltic pump, and solution exchanges were performed by VC-6 six-channel valve controller (Warner Inst., USA). Conventional two-electrode (3–10 MΩ) voltage-clamp techniques (Axoclamp2B, TEVC mode) were employed to measure agonist-activated currents as reported73 at room temperature(20 ± 2 °C). To characterize membrane and action potentials, we used a bridge mode of Axoclamp2B with borosilicate microelectrodes (tip resistance: 10–18 MΩ, with 0.5 M KCl, 2 M K-Acetate, and 5 mM HEPES, pH = 7.2).
Data availability
All data are contained within the article as well in Supplementary Information.
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Acknowledgements
We thank Dr. T.Ha and E. Bobkova for help with cloning and in situ hybridization. We also thank two anonymous reviewers for their highly valuable comments and suggestions. The manuscript is dedicated to Geoffrey Burnstock (1929–2020), who discovered and promoted purinergic signaling over decades.
Funding
This work was supported by the Human Frontiers Science Program (RGP0060/2017) and National Science Foundation (1146575, 1557923, 1548121, 1645219) Grants to L.L.M. Research reported in this publication was supported by the National Institute of Neurological Disorders and Stroke of the National Institutes of Health under Award Number R01NS114491 (to L.L.M.). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. D.R. was supported by Russian Ministry of Science and High Education (agreement 075-15-2020-801); J. G. was supported by Hungarian Government Balaton Limnological Institute grant.
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All authors had access to the data in the study and take responsibility for the integrity of the data and the accuracy of the data analysis. Research design, acquisition of data: all authors. Molecular data, expression and RNA-seq: A.B.K., L.L.M.; Protein modeling and analysis: D.R. Pharmacological tests: J.G.; Analysis and interpretation: all authors: Manuscript writing: L.L.M. Funding: L.L.M. All authors reviewed the manuscript.
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Györi, J., Kohn, A.B., Romanova, D.Y. et al. ATP signaling in the integrative neural center of Aplysia californica. Sci Rep 11, 5478 (2021). https://doi.org/10.1038/s41598-021-84981-5
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DOI: https://doi.org/10.1038/s41598-021-84981-5
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