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Inhibition of mutant troponin C activity by an intra-domain disulphide bond

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Abstract

Triggering of contraction in striated muscles involves a conformational transition in the N-terminal domain of troponin C, the calcium-binding component of thin filaments. We have designed a mutant troponin C in which the key conformational transition and the calcium-regulatory activity are reversibly blocked by the formation of a disulphide bridge. Our results may be applicable to other proteins of the same family of calcium-binding proteins.

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Authors and Affiliations

  1. Department of Muscle Research, Boston Biomedical Research Institute, Boston, Massachusetts, 02114, USA

    Zenon Grabarek, Ruo-Ying Tan, **g Wang, Terence Tao & John Gergely

  2. Department of Neurology and ‡Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, 02115, USA

    Terence Tao & John Gergely

  3. Neurology Service, Massachusetts General Hospital, Boston, Massachusetts, 02114, USA

    John Gergely

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  1. Zenon Grabarek
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  2. Ruo-Ying Tan
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  3. **g Wang
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  4. Terence Tao
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  5. John Gergely
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Grabarek, Z., Tan, RY., Wang, J. et al. Inhibition of mutant troponin C activity by an intra-domain disulphide bond. Nature 345, 132–135 (1990). https://doi.org/10.1038/345132a0

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