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High-resolution genetic map** of QTL governing resistance to corn leaf aphid, Rhopalosiphum maidis (Fitch) in barley

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Abstract

A map** population, recombinant inbred lines (RILs), was developed from the cross of aphid susceptible cv. PL426 and resistant genotype BK 9816 to identify genetic loci conferring corn leaf aphid (CLA) Rhopalosiphum maidis (Fitch) resistance in barley. RILs showed wide range of aphid infestation (2.2–65.0 aphids/tiller) indicating quantitative inheritance of aphid resistance. All the traits related to aphid resistance, viz. aphid fecundity, chlorophyll content and tiller dry weight of plants, also showed quantitative variation. RILs were further genotyped by sequencing to identify SNPs covering whole barley genome. A total of 605 high-quality bin-mapped SNPs were positioned in seven linkage groups with a total map length of 1208.9 cM and average marker distance of 2.02 cM. Composite interval map** identified five different quantitative trait loci (QTLs) on chromosomes 1H, 3H, 5H and 6H associated with aphid resistance and related traits. Two QTLs on chromosome 6H, QCLA.pau-6H.1 and QCLA.pau-6H.2, were mapped at genetic intervals of 1.7 cM and 4.9 cM, respectively. QCLA.pau-6H.1 explained 9.76 percent of phenotypic variation for aphid count and 12.78 percent for aphid score. Similarly, QCLA.pau-6H.2 explained 10.96 percent of phenotypic variation for aphid score. The QTL for chlorophyll content and tiller dry weight spanned the same region of QCLA.pau-6H.2, explained 13.08 and 13.20 percent phenotypic variation, respectively. Another QTL associated with chlorophyll content was mapped on chromosome 3H at 3.6-cM interval explained 12.90 percent phenotypic variation. In addition, QTL regions were also identified for aphid resistance and related traits on chromosome 1H and 5H.

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Data availability

The SRA data for all the individual along with parental lines are available under accession number PRJNA755816 in the NCBI SRA database (http://www.ncbi.nlm.nih.gov/sra/PRJNA755816).

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Acknowledgements

Authors express their gratitude to Head, Department of Plant Breeding and Genetics and Director, School of Agricultural Biotechnology for providing facilities to carry out the study.

Funding

The financial support provided by the Department of Biotechnology, Ministry of Science and Technology, Government of India, in the form of project grant BT/PR9656AGR/2/870/2013 is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, Punjab, 141 004, India

    Beant Singh, Dharminder Bhatia, Deepika Narang & Rajwinder Kaur

  2. School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, Punjab, 141 004, India

    Parveen Chhuneja

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  1. Beant Singh
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  2. Dharminder Bhatia
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  3. Deepika Narang
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  4. Rajwinder Kaur
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  5. Parveen Chhuneja
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Contributions

BS and PC conceived and designed this research; BS and RK performed the experiment; DB and DN analyzed the data; BS, PC, DB and DN wrote the manuscript.

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Correspondence to Beant Singh.

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Informed consent was obtained from all individual participants included in the study.

Additional information

Communicated by Mian Abdur Rehman Arif.

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Singh, B., Bhatia, D., Narang, D. et al. High-resolution genetic map** of QTL governing resistance to corn leaf aphid, Rhopalosiphum maidis (Fitch) in barley. CEREAL RESEARCH COMMUNICATIONS 51, 379–389 (2023). https://doi.org/10.1007/s42976-022-00317-7

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