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A novel major gene on chromosome 6H for resistance of barley against the barley yellow dwarf virus

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Abstract

In a map** population derived from the Ethiopian barley line L94 × Vada, natural infection by barley yellow dwarf virus (BYDV) occurred. While line L94 hardly showed symptoms, Vada was severely affected. The 103 recombinant inbred lines segregated bimodally. The major gene responsible for this resistance mapped to chromosome 6H. We propose to name the locus Ryd3. A subset of recombinant inbred lines, L94, and Vada were planted in a subsequent field test which confirmed the previous field observations. Double antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISA) indicated that the epidemic was due to a combination of the serotypes BYDV-PAV and BYDV-MAV. In the accessions with the least BYDV symptoms no virus was detected, justifying the consideration of the gene as conferring true resistance rather than tolerance to these viruses. In a laboratory/gauze house trial a near-isogenic line carrying the Vada chromosome 6H fragment in an L94 background was affected as much as Vada. The effect of Ryd3 was quantified, and compared with that of the only other known major gene for resistance to BYDV, Ryd2, which is also of Ethiopian origin and is located on chromosome 3H. Both genes seemed to reduce the chance of the viral isolate used in this study to establish infection. In plants in which it became established, the virus concentration reached a similar level as in susceptible accessions, but with less dramatic symptom development. Inoculated plants in which the virus failed to multiply tended to show an increase in the number of ears per plant, resulting in higher grain yield per plant. Ryd3 co-segregates with several PCR-based molecular markers that may serve for marker assisted selection.

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References

  • Baltenberger DE, Ohm HW, Foster JE (1987) Reactions of oat, barley and wheat to infection with barley yellow dwarf isolates. Crop Sci 27:195–198

    Google Scholar 

  • Banks PM, Waterhouse PM, Larkin PJ (1992) Pathogenicity of three RPV isolates of barley yellow dwarf virus on barley, wheat and wheat alien addition lines. Ann Appl Biol 121:305–314

    Google Scholar 

  • Berloo R van (1999) GGT: software for the display of graphical genotypes. J Hered 90:328–329

    Article  Google Scholar 

  • Berloo R van, Aalbers H, Werkman A, Niks RE (2001) Resistance QTL confirmed through development of QTL-NILs for barley leaf rust resistance. Mol Breed 8:187–195

    Article  Google Scholar 

  • Burnett PA, Comeau A, Qualset CO (1995) Host plant tolerance or resistance for control of barley yellow dwarf. In: D’Arcy CJ, Burnett PA (eds) Barley yellow dwarf. Forty years of progress. APS, St Paul, pp 321–343

    Google Scholar 

  • Catherall PL, Jones AT, Hayes JD (1970) Inheritance and effectiveness of genes in barley that condition tolerance to barley yellow dwarf virus. Ann Appl Biol 65:153–161

    Google Scholar 

  • Chalhoub BA, Sarrafi A, Lapierre HD (1995) Partial resistance in the barley (Hordeum vulgare L.) cultivar Chikurin Ibaraki 1 to two PAV-like isolates of barley yellow-dwarf virus: allelic variability at the Yd2 gene locus. Plant Breed 114:303–307

    Google Scholar 

  • Collins NC, Paltridge NG, Ford CM, Symons RH (1996) The Yd2 gene for barley yellow dwarf virus resistance maps close to the centromere on the long arm of barley chromosome 3. Theor Appl Genet 92:858–864

    Article  CAS  Google Scholar 

  • Ford CM, Paltridge NG, Rathjen JP, Moritz RI, Simpson RJ, Symons RH (1998) Rapid and informative assays for Yd2, the barley yellow dwarf virus resistance gene, based on the nucleotide sequence of a closely linked gene. Mol Breed 4:23–31

    Article  CAS  Google Scholar 

  • Friedt W, Scheurer KS, Huth W, Habekuß A, Ordon F (2003) Genetic analysis of BYDV-tolerance in barley (Hordeum vulgare L.). J Plant Dis Prot 110:278–286

    CAS  Google Scholar 

  • Jefferies SP, King BJ, Barr AR, Warner P, Logue SJ, Langridge P (2003) Marker-assisted backcross introgression of the Yd2 gene conferring resistance to barley yellow dwarf virus in barley. Plant Breed 122:52–56

    Article  CAS  Google Scholar 

  • Jørgensen JH (1992) Discovery, characterization and exploitation of Mlo powdery mildew resistance genes in barley. Euphytica 63:141–152

    Google Scholar 

  • Künzel G, Korzun L, Meister A (2000) Cytologically integrated physical restriction fragment length polymorphism maps for the barley genome based on translocation breakpoints. Genetics 154:397–412

    PubMed  Google Scholar 

  • Le Gouis J, Devaux P, Werner K, Hariri D, Behrman N, Béghin D, Ordon F (2004) Rym15 from the Japanese cultivar Chikurin Ibaraki1 is a new barley mild mosaic virus (BaMMV) resistance gene mapped on chromosome 6H. Theor Appl Genet 108:1521–1525

    Article  PubMed  Google Scholar 

  • McKirdy SJ, Jones RAC, Nutter FW Jr (2002) Quantification of yield losses caused by Barley yellow dwarf virus in wheats and oats. Plant Dis 86:769–773

    Google Scholar 

  • Miller WA, Rasochova L (1997) Barley yellow dwarf viruses. Annu Rev Phytopathol 35:167–190

    Article  CAS  PubMed  Google Scholar 

  • Niks RE, Fernández E, Haperen B van, Bekele-Aleye B, Martínez F (2000) Specificity of QTLs for partial and non-host resistance of barley to leaf rust fungi. Acta Phytopathol Hung 35:13–21

    CAS  Google Scholar 

  • Parlevliet JE, Ommeren A van (1984) Interplot interference and the assessment of barley cultivars for partial resistance to leaf rust, Puccinia hordei. Euphytica 33:685–697

    Google Scholar 

  • Proll E, Richter J, Hamann U, Stanarius A, Eisenbrandt K, Spaar D (1984) Kartoffelblattrollvirus (potato leaf roll virus): Reinigung und Herstellung von Antiseren. Arch Phytopathol Plant Prot 20:1–13

    Google Scholar 

  • Qi X, Stam P, Lindhout P (1998a) Use of locus specific AFLP markers to construct a high density molecular map in barley. Theor Appl Genet 96:376–384

    Article  CAS  Google Scholar 

  • Qi X, Niks RE, Stam P, Lindhout P (1998b) Identification of QTLs for partial resistance to leaf rust (Puccinia hordei) in barley. Theor Appl Genet 96:1205–1215

    Article  CAS  Google Scholar 

  • Ramsay L, Macaulay M, Ivanissevich S degli, MacLean K, Cardle L, Fuller J, Edwards KJ, Tuvesson S, Morgante M, Massari A, Maestri E, Marmiroli N, Sjakste T, Ganal M, Powell W, Waugh R (2000) A simple sequence repeat-based linkage map of barley. Genetics 156:1997–2005

    CAS  PubMed  Google Scholar 

  • Ranieri R, Lister RM, Burnett PA (1993) Relationship between barley yellow dwarf virus titer and symptom expression in barley. Crop Sci 33:968–973

    Google Scholar 

  • Rasmusson DC, Schaller CW (1959) The inheritance of resistance in barley to the yellow-dwarf virus. Agron J 51:661–664

    Google Scholar 

  • Regenmortel MHV van, Fauquet CM, Bishop DHL (2000) Virus taxonomy. Classification and nomenclature of viruses. In: Seventh report of the international committee on taxonomy of viruses. Academic, San Diego, pp 775–784

  • Schaller CW (1984) The genetics of resistance to barley yellow dwarf virus in barley. In: Burnett PA (ed) Barley yellow dwarf. A proceedings of the workshop. CIMMYT, Mexico, pp 93–99

  • Schaller CW, Rasmusson DC, Qualset CO (1963) Sources of resistance to the yellow-dwarf virus in barley. Crop Sci 3:342–344

    Google Scholar 

  • Schaller CW, Qualset CO, Rutger JN (1964) Inheritance and linkage of the Yd2 gene conditioning resistance to the barley yellow dwarf disease in barley. Crop Sci 4:544–548

    Google Scholar 

  • Scheurer KS, Huth W, Friedt W, Ordon F (2000) First results on BYDV-tolerance in barley estimated in pot experiments. J Plant Dis Prot 107:427–432

    Google Scholar 

  • Scheurer KS, Friedt W, Huth W, Waugh R, Ordon F (2001) QTL analysis of tolerance to a German strain of BYDV-PAV in barley (Hordeum vulgare L.). Theor Appl Genet 103:1074–1083

    Article  CAS  Google Scholar 

  • Skaria M, Lister RM, Foster JE, Shaner G (1985) Virus content as an index of symptomatic resistance to barley yellow dwarf virus in cereals. Phytopathology 75:212–216

    Google Scholar 

  • Spaner D, Shugar LP, Choo TM, Falak I, Briggs KG, Legge WG, Falk DE, Ullrich SE, Tinker NA, Steffenson BJ, Mather DE (1998) Map** of disease resistance loci in barley on the basis of visual assessment of naturally occurring symptoms. Crop Sci 38:843–850

    Google Scholar 

  • Stam P, Bos I, Qi X, Niks RE (1997) QTL map** of yield and yield-related traits in a wide cross of spring barley (Hordeum vulgare L.). Advances in biometrical genetics. In: Krajewski P, Kaczmarek Z (eds) Proceedings of 10th meeting of the EUCARPIA section biometrics in plant breeding. Poznan, pp 277–280

  • Suneson CA (1955) Breeding for resistance to barley yellow dwarf virus in barley. Agron J 47:283

    Google Scholar 

  • Suneson CA, Ramage RT (1957) Studies on the importance of the yellow-dwarf virus. Agron J 49:365–367

    Google Scholar 

  • Too**da T, Broers LH, Chen XM, Hayes PM, Kleinhofs A, Korte J, Kudrna D, Leung H, Line RF, Powell W, Ramsay L, Vivar H, Waugh R (2000) Map** quantitative and qualitatitve resistance genes in a doubled haploid population of barley (Hordeum vulgare) Theor Appl Genet 101:580–589

    Article  CAS  Google Scholar 

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Acknowledgements

We acknowledge the expert technical assistance in the field trials by Anton Vels. We thank Thierry Marcel for providing additional marker information on the map** population.

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Authors and Affiliations

  1. Graduate School of Experimental Plant Sciences, Department of Plant Breeding, Wageningen University, P.O. Box 386, 6700, Wageningen, The Netherlands

    R. E. Niks & B. Bekele

  2. Federal Centre for Breeding Research on Cultivated Plants, Institute of Epidemiology and Resistance, Theodor-Roemer-Weg 4, 06449, Aschersleben, Germany

    A. Habekuß & F. Ordon

Authors
  1. R. E. Niks
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  2. A. Habekuß
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  3. B. Bekele
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  4. F. Ordon
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Correspondence to R. E. Niks.

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Communicated by P. Langridge

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Niks, R.E., Habekuß, A., Bekele, B. et al. A novel major gene on chromosome 6H for resistance of barley against the barley yellow dwarf virus . Theor Appl Genet 109, 1536–1543 (2004). https://doi.org/10.1007/s00122-004-1777-7

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