Abstract
Pectobacterium brasiliense is an important bacterial species that causes soft rot disease in a wide range of host plants including potatoes worldwide. In 2018, seed potato tubers were collected to verify the presence and characterize strains of P. brasiliense in Turkey. Bacterial isolation from soft-rotted seed tubers was performed on a CVP medium, and we found that 18 of 34 strains (55%) belonged to the P. brasiliense species. All of them produced 322 bp products with Br1f/L1r primers in the PCR assay. Partial nucleotide sequences of the housekee** genes mdh and recA confirmed the result obtained with the species-specific primers. Phylogenetic analysis of the recA gene sequences allowed the strains to be grouped into a cluster divided into two clades distinct from other Pectobacterium species. There was a difference between the tested strains for their ability to macerate potato tubers tissue. Four Turkish P. brasiliense strains were determined to be highly virulent on potato tubers, which caused soft rot symptoms on plants of 17 species of 8 families. Multiple strains also caused soft rot on harvested plant organs (leaves, head, and bulb) and necrosis on sugar beet roots and eggplant fruits, except strain Pbr5, whose pathogenicity was limited. This is the first isolation of P. brasiliense from infected seed potato tubers in Turkey and the first detailed report showing the host range of domestic strains.
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Acknowledgements
The author would like to sincerely thank Prof. Dr. Ewa Lojkowska for supplying the reference cultures from the Polish IFB collection (the Intercollegiate Faculty of Biotechnology University of Gdansk and Medical University of Gdansk, Gdansk, Poland) and Dr. A. Demirci for diligent proofreading of this manuscript.
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This study was supported by a financial grant from the project management office, Yozgat Bozok University, project no. 6602c-ZF/21–472.
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MO conducted the research, survey, isolation, identification, analyzed and discussed the results, and writing—review, editing, ARU performed only pathogenicity experiment for host range analysis and all authors read and approved the final manuscript.
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Öztürk, M., Umar, A.R. Occurrence, identification, and host range of Pectobacterium brasiliense causing soft rot on seed potato tubers in Turkey. J Plant Dis Prot 130, 1–12 (2023). https://doi.org/10.1007/s41348-022-00675-8
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DOI: https://doi.org/10.1007/s41348-022-00675-8