Abstract
Ecological studies of common brushtail possums (Trichosurus vulpecula) in their extant range have been limited by technology and the species’ nocturnal habit. However, camera traps now allow the investigation of possum ethology without observer interference. Here, we analysed terrestrial possum activity patterns using a large dataset collected over 3 years from 133 camera traps in mesic eucalypt woodland and open forest in three national parks on the New England Tablelands, New South Wales, Australia. We investigated how weather and moonlight intensity influenced possum activity patterns throughout the night, and across seasons and years, by using the timestamps assigned to each detection by the camera trap. Terrestrial possum activity increased as ambient temperatures decreased in autumn and peaked in winter when females were rearing offspring. Nightly possum detections decreased significantly with rain and increasing mean temperature. Possums were almost exclusively nocturnal, with most terrestrial activity earlier in the evening in winter and later at night in summer. During longer nights, higher temperatures also delayed activity. While nightly detection rates were not affected by lunar phase, possums preferred parts of the night with the highest moonlight intensity, and this effect was stronger on brighter nights. Overall, brushtail possums were most active on the ground when temperatures were mild and moonlight bright, presumably assisting foraging and predator avoidance, and during the breeding season; they avoided rain. These patterns suggest that reproduction, thermoregulation and risk of predation strongly shape the nocturnal activity cycle. Furthermore, our research adds to the evidence that camera traps can help greatly expand our knowledge of the ecology and behaviour of nocturnal mammals.
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Introduction
The common brushtail possum, Trichosurus vulpecula (Kerr, 1792), is a medium-sized (1.2–4.5 kg) marsupial endemic to Australia and widely distributed, with five subspecies across the continent (Jackson and Groves 2015). It falls into the category of ‘critical weight range’ — between approximately 35 and 5500 g — in which the highest risk of mammal decline and extinction has been observed in Australia (Burbidge and McKenzie 1989). Following European settlement, some urban and agricultural regions have maintained robust populations, and possums have successfully adjusted to living in cities (Eymann et al. 2006). At the same time, the species has suffered a decline as well as local and regional extinction throughout much of its range, particularly in central Australia, tropical savannas and parts of inland New South Wales and Western Australia. Its decline has been attributed primarily to land clearing, hunting, predation by introduced predators (dingoes, Canis familiaris; European red fox; Vulpes vulpes; and feral cat, Felis catus) and increasing aridity (Kerle et al. 1992; How and Hillcox 2000; Goldingay and Jackson 2004; Stobo-Wilson et al. 2019). There have been attempts to reintroduce brushtail possums, with varied success, into sites within its historic range, such as Ikara-Flinders Ranges National Park in South Australia (Bannister et al. 2020) and Wadderin Sanctuary in Western Australia (Short and Hide 2014).
Successful management of wildlife species relies on knowledge of their biology, ecology and ethology. Brushtail possum ecology and ethology have long been studied (Tyndale-Biscoe 1955; Dunnet 1956; Kerle et al. 1992; Marsh et al. 2003; Mella et al. 2014). Research has been particularly focused on New Zealand where the species was introduced in the nineteenth century and subsequently became a major pest, damaging plantations and native forests, preying on native bird species and posing a risk as a vector of bovine tuberculosis (Paterson et al. 1995; Montague 2000; Nugent and Sweetapple 2000; Nugent et al. 2001, 2015; Glen et al. 2012). Nevertheless, studying activity patterns has been limited by the species’ nocturnal habits and available technologies. VHF radio collars and GPS tags have provided further insight (Ball et al. 2005; Harper 2006; Whyte et al. 2014), but these methods are resource- and time-intensive and tend to provide detailed information on a subset of individuals rather than describing populations. Overall, the effects of environmental variables on fine-scale temporal activity and long-term seasonal changes are still largely unknown. Recent advances in wildlife monitoring with camera traps allow continuous recording of animal activity at a landscape scale, providing both spatially and temporally explicit information at a population level (Meek et al. 2014; Rovero and Zimmerman 2016). Camera traps combine the benefits of high temporal resolution and long-term monitoring, allowing investigation of seasonal changes in activity as well as fine-scale diel activity patterns. To our knowledge, there is no long-term dataset from continuous monitoring of common brushtail possum activity at landscape scale over multiple years in their native range in Australia.
Here we analysed camera trap data collected over 3 years to detect terrestrial activity patterns of brushtail possums in the New England Tablelands at different temporal scales — nightly, and within and between seasons and years. We also sought to identify the influence of environmental factors, such as weather conditions and moonlight illumination, on these patterns. We did not attempt to estimate population densities at our study sites but rather focused on activity indices, analysing general patterns at a landscape scale.
We recognise that our results represent patterns of possum activity on the ground, but their overall activity patterns might differ. However, no other research method allows for continuous monitoring of animal activity on a landscape scale across multiple seasons. There have been attempts to survey Australian arboreal fauna at tree canopy level (e.g. Cotsell and Vernes 2016), but placing cameras on tree branches or directed at tree hollows restricts the effective detection area of each camera to a branch or hollow. Because possums use multiple dens within their home range and rarely share dens (Paterson et al. 1995), a substantially higher number of canopy cameras would be required to collect a comparable dataset of arboreal activity.
Material and methods
Study area
Our study was conducted in the New England Tablelands in north-eastern New South Wales, Australia (Fig. 1). This region encompasses the Great Escarpment, with steep, rugged gorges and boulder-strewn slopes in the east (1500 m a.s.l.) and undulating plateaus in the west (600 m a.s.l.). The four monitoring sites were situated towards the east of the plateau and were: Tabletop (30°53ʹ30ʺ S, 151°52ʹ50ʺ E) and Front Tableland (30°59ʹ3ʺ S, 152°7ʹ8ʺ E) in Oxley Wild Rivers National Park (NP), Paddys Land (30°4ʹ49ʺ S, 152°12ʹ0ʺ E) in Guy Fawkes River NP and Petroi (30°35ʹ12ʺ S, 152°19ʹ27ʺ250 E) in New England NP (Fig. 1). Vegetation is a combination of sclerophyll forests and woodlands with pockets of subtropical dry rainforest in gullies and gorges. Dominant vegetation types vary between sites, with dry sclerophyll forests at Tabletop, a combination of dry and wet sclerophyll forests at Front Tableland and Paddys Land and wet sclerophyll and rainforest at Petroi (Office of Environment and Heritage 2016). The altitudinal range of camera locations was similar for all study sites, with the lowest cameras at 854–916 m a.s.l. and the highest at 1104–1281 m a.s.l. The climate is temperate with the highest temperatures in January and the lowest in July. Rainfall is distributed throughout the year, with the highest rainfall in summer, from November to February (Bureau of Meteorology (BOM) 2020). The four study sites were located within 100 km and, due to topography, represented comparable but sufficiently different climatic and weather conditions (Table 1) to test the effects of environmental factors on activity.
The study sites were located in extensive areas of native vegetation, with distances ranging from 0 to 7.5 km to agricultural land, predominantly sheep and cattle farms, where dingo and red fox populations are controlled to varying degrees by toxic baiting, trap**, shooting and exclusion boundary fences. During the study period, two of the study sites (Tabletop and Front Tableland) were also subjected to aerial predator baiting programmes, typically undertaken in May to June by the NSW National Parks and Wildlife Service (NPWS).
Camera trap monitoring
A permanent camera trap monitoring network consisting of 20–60 Reconyx Hyperfire HC600 cameras (RECONYX LLC, Holmen, WI, USA) per site was established in the four study sites. From a random starting point, camera traps were placed on perpendicular poles located at ~ 1 km spacing along management trails. Camera traps were approximately 50 cm above the ground, faced along the trail and angled at ~ 22.5° towards the axis of the trail. As this camera placement was optimised for the detection of introduced predators (Ballard et al. 2014), we tested it for reliable detection of slightly smaller possums. For a limited time, a second camera was fitted 25 cm above the ground for a subset of cameras (approximately one-third of all cameras at two study sites). While the lower mounted cameras were more effective in detecting possums, the cameras mounted at the standard height of 50 cm detected possums consistently albeit at a reduced rate; our data was, therefore, a representative subset of all possum movements on the ground (M.K.Ś. unpublished). We did not need to know the exact numbers of detections because we assumed that the detection probability was constant among camera traps. Because our dataset is large (> 10,000 independent possum detections), collected over a long period (3 years) and the ratio of detections was consistent between paired cameras set at different heights, we are confident that our detections were drawn randomly from all real events.
Cameras were serviced approximately every 2 months when batteries and SD cards were replaced. All photos were then downloaded to a PC, tagged to species level and filtered for those containing T. vulpecula. A threshold of 5 min was applied to define individual activity events (i.e. subsequent detections within a 5-min period were ignored). These activity events (hereafter referred to as ‘events’) were used in subsequent analyses (Table 2).
Environmental variables
Temperature
For each site, the temperature information encoded in metadata of each picture taken by the camera trap was used to initially calculate mean temperature values for 1-h intervals from all available readings in that hour. On average, 3.6 readings (SD = 3.63) were recorded per 1-h block, and at least one reading was recorded for 50,362 out of the 70,462 one-hour blocks. Missing values were interpolated, and data further condensed to calculate an average value for the 12-h block between 18:00 and 06:00, representing the average nightly temperature. The intermediate step of calculating 1-h blocks was used because motion triggers were often clustered (e.g. animals grazing continuously in front of a camera), and such a cluster would skew a mean towards values recorded in that cluster.
Sunlight and moonlight
We defined night as the period between sunset and sunrise. Each event was placed in the context of astronomical events (such as sunrise, sunset, moonrise, moonset), and these were calculated using the package ‘suncalc’ (Agafonkin and Thieurmel 2018). To account for seasonal changes in the length of the night, as well as shifts in the time of sunset and sunrise, we standardised the time of each event by converting it to a relative position of that event within a night. Each nocturnal observation took a fractional value between 0 (at sunset) and 1 (at sunrise), with a value of 0.5 for astronomical midnight.
Furthermore, moonlight illumination was calculated as the ambient intensity of moonlight on the ground using a custom model, taking into account brightness of the lunar disc, distance to the moon, position of the moon in the sky and light propagation in the atmosphere (Śmielak 2023). For each night at each of the study sites, we calculated mean moonlight intensity and the time of the lunar noon (the point when the moon is at its highest over the horizon and thus the brightest). The moonlight illumination model did not consider cloud cover; therefore, an interaction variable (moonlight intensity × mean cloud cover) was included in the global model. Time of lunar noon was standardised relative to the duration of each respective night.
Rainfall and cloud cover
Rainfall records were obtained from historical weather data services (Bureau of Meteorology (BOM) 2020), with the distance of each camera trap to the nearest weather station ranging from 1.7 to 24.3 km. Daily measurements were taken around 09:00 h and represented total rainfall over the previous 24 h. Across the four sites, rainfall records were unavailable for 218 out of 4146 days, and those days were removed from analysis.
Cloud cover data were obtained from the NCEP/NCAR Reanalysis 1 project using the R package RNCEP (Kemp et al. 2020). For each study site, cloud cover data for the nearest grid cell was downloaded in a 6-h series, and an average cloud cover for each night was calculated as a weighted mean (weights were 0.25 for 18:00 h, 0.5 for 00:00 h and 0.25 for 06:00 h).
Statistical analysis
All analyses were performed using R Statistical Software (version 4.2.2, R Core Team 2022) . We identified candidate models by applying the ‘dredge’ function in the MuMIn package (Bartoń 2020) to a global model containing all variables and interactions. The best candidate models were chosen based on the Akaike information criterion (AIC), and models with ΔAIC < 2 (Symonds and Moussalli 2011) are presented in Tables 3 and 4. All numerical explanatory variables were standardised by subtracting the mean value and dividing by one standard deviation (Schielzeth 2010). Models were fitted using R package ‘glmmTMB’ (Magnusson et al. 2020).
To analyse the influence of environmental conditions on daily detection rates, we fitted a generalised linear model (GLM) with a log link function and a negative binomial error distribution. The response variable was the number of events in a 24-h period (from noon to noon) across all cameras for a given site. Explanatory variables in the global model were mean temperature at night, rainfall, night length, mean moonlight illumination, mean cloud cover and the interactions between mean temperature and mean cloud cover, mean temperature and night length, mean temperature and rainfall and mean moonlight illumination and mean cloud cover. Because of the length of the study, long-term changes in detection rates, which varied between sites, were present in our data. We accounted for this temporal trend, as well as the different number of cameras per site, by including the interaction between two explanatory variables — day since the beginning of the study and site —in the model. Three candidate models with ΔAIC < 2 were identified, and they were averaged using model.avg() function in MuMIn to produce the final model.
To analyse the influence of environmental conditions on the temporal distribution of possum activity, we removed seven anomalous diurnal data points from the dataset and fitted a generalised linear mixed model (GLMM). The response variable, standardised diel time of activity, took values from 0 to 1 (see above). Therefore, a beta distribution with a logit link function was used in the model. Explanatory variables in the global model were mean temperature at night, night length, time of lunar noon, mean moonlight intensity, mean cloud cover and interactions between mean temperature at night and night length, mean temperature at night and mean cloud cover and the time of lunar noon and mean moonlight intensity. Camera trap identity was included as a random variable, and three candidate models with ΔAIC < 2 were identified and averaged using model.avg() function in MuMIn to produce the final model.
Results
Detection rates
Over the 3 years, 10,037 common brushtail possum activity events were recorded with an average of 7.1 events per 100 camera trap nights (Table 2). The lowest mean detection rate was recorded at Tabletop (3.4 events per 100 camera trap nights) and the highest at Paddys Land (15.3 events per 100 camera trap nights). We observed seasonal patterns with higher nightly detection rates in winter than in summer. This was also true for nocturnal hourly detection rates, indicating that higher daily detection rates were not due to a wider window of detection on longer winter nights (Fig. 2).
Daily (per 100 camera trap nights; left) and hourly (per 100 camera trap hours, night only; right) detection rates (grey) and moving average (solid line) for each study site. For all sites, there was an increase in activity mid-year (winter) and a decrease around the new year (summer), to varying degrees. Moving average calculated using ‘loess’ function and moving window of 0.3
The mean nightly temperature was 10.9 °C (range − 2.0–27.2 °C), and the mean cloud cover was 39% (range 0–99%). The mean daily rainfall recorded was 2.4 mm (range 0–158 mm), and no rainfall was recorded on 66% of the nights with data.
Possum daily detection rates were significantly affected by three two-way interactions, all involving mean nightly temperature: mean cloud cover, night length and rainfall (Table 5). Possum terrestrial activity declined with increasing mean nightly temperature but to a much greater degree on cloudy nights than on cloudless nights (Fig. 3c). The impact of increasing nightly temperature on declining possum activity was only evident on rain-free nights (Fig. 3a) and was much greater on short summer nights than in midwinter, when the effect was almost but not significantly reversed (Fig. 3b). Modest rainfall nullified the effect of nightly temperature on possum activity, and heavy rain reversed the pattern, with significantly more possum activity on the warmest nights with heavy rain and virtually none on the coldest, very wet nights.
Temporal distribution of terrestrial activity
Common brushtail possum activity was almost exclusively nocturnal, with 10,030 of the 10,037 events recorded at night. There was a seasonal pattern observed across all study sites, with activity being recorded, on average, earlier during the night in winter than in summer (Fig. 4).
The standardised time of possum activity at four study sites. Horizontal lines represent sunset (y = 0) and sunrise (y = 1). Black line represents the moving mean of the time of activity calculated using ‘loess’ function and moving window of 0.3, with the mean time of detection closer to sunset in winter, and closer to sunrise in summer. Shading represents density of observations
The time of nocturnal possum activity was significantly influenced by three two-way interactions: between mean temperature at night and night length; between mean temperature at night and cloud cover; and between time of lunar noon and mean moonlight intensity (Table 6). The effect of night length was inversely related to the timing of possum activity, with possums more active earlier in the evening in midwinter than in midsummer (Fig. 5a). However, this effect was modulated by the mean temperature at night, with possums active earlier in the evening on cold than on warmer winter evenings. In midsummer, the mean temperature had no effect on possums becoming active later in the night. The effect of mean temperature was also modulated by mean cloud cover: on colder nights, possums were active later when there were clouds, and the opposite was true for the warmest nights (Fig. 5b). The timing of possum activity was also positively influenced by the time of lunar noon when moonlight is most intense, with possums most active early in the evening when the lunar noon was early but delaying their activity until later in the night when the lunar noon was late (Fig. 5c). This relationship was strongest on bright nights, while on nights with no moonlight, the time of lunar noon had no impact on the timing of possum activity.
Discussion
Seasonal changes
During the study, possums were more active on the ground between autumn and spring when ambient temperature was the lowest. Higher detection rates can be explained by a likely increase in the number of individuals, greater possum activity due to increased energy requirements during cold winter weather and/or increased mobility when searching for mates. Reproduction in possums in temperate regions appears to be moderately seasonal with a birth pulse in autumn (April to May) followed in some years by a second, springe birth pulse (Cowan 2014). Similar patterns have been observed previously at one of our study sites (Clinchy et al. 2004). Furthermore, pregnancy and rearing offspring combined with lower temperature in winter increase energetic requirements, likely explaining the correlation between seasonal temperatures and detection rates. During the autumn breeding season, activity is extended (Paterson et al. 1995), and males have been found to lose body condition (Gilmore 1969), likely due to longer movements as they increase their home range (Green 1984). The cyclic change in detection rate is most likely primarily due to seasonal changes in population size through recruitment, mortality and migrations, which is behaviourally modulated by greater mobility associated with reproduction, greater energetic requirements of rearing offspring and colder ambient temperatures.
Weather
Without rain, possums were more active on the ground on colder nights, which was expected given that increased movement increases thermogenesis for behavioural thermoregulation (Terrien et al. 2011). The lower critical temperature (the temperature below which the basal rate of heat production does not compensate for heat loss to the environment, therefore incurring thermoregulatory costs) for brushtail possums in still air is between 7 and 10 °C (van den Oord et al. 1995). Hence, moderately cool nights incur little energetic penalty. The situation changes significantly with rain and wind. Simulated rain by itself increased the lower critical temperature by 6 °C and in conjunction with wind by 8 °C (van den Oord et al. 1995). We observed a strong negative impact of rainfall on activity on cool nights, which is consistent with previous research (Herbert and Lewis 1999). Effect of rainfall was, however, minimal on the warmest nights, showing that temperature can minimise the energetic penalty of rain on thermoregulation. The sugar glider (Petaurus breviceps), a related smaller possum species, is significantly less active and often enters torpor on cold and rainy nights (Körtner and Geiser 2000). Therefore, although possums were more active on colder nights, which presumably increased thermogenesis, rain likely increased the costs of thermoregulation to a point where it was difficult to offset it by increased food intake. As a result, reducing activity, and presumably denning, likely became the preferred strategy on cold rainy nights.
Ambient temperatures are rarely constant throughout the night. Generally, night temperatures are the highest at sunset and decrease until sunrise as heat is radiated from the ground. On cloudy nights, some of the radiated heat is reflected towards the ground, which means temperatures drop more slowly. While this could be advantageous on cold nights, on warm nights, it could mean that temperature can potentially stay uncomfortably high for longer — an effect that was especially pronounced during shorter summer nights. Possums can tolerate ambient temperatures up to 40 °C (Halse and Rose 1988), but evaporative cooling, respiratory rate and metabolic rate begin to increase rapidly above 25 °C in resting possums (upper critical temperature). Activity would further lower this threshold as an activity-related increase in metabolic rate requires enhanced heat dissipation, which in turn requires more energy and water use. Moreover, high temperatures can suppress food intake as they reduce hepatic detoxification capacity (Beale et al. 2022). Hence, warmer ambient temperatures can be as problematic as cold and rainy conditions. As a result, on cold nights with clouds, the optimal window of activity widened, whereas on warm clear nights it shrank, and detection rate decreased. This effect was further reinforced on shorter nights or mitigated when the night was sufficiently long.
Changes in ambient climatic conditions over the course of the evening meant that activity varied throughout the night. Previous studies have shown that possums exposed to outdoor conditions in pens commenced activity after sunset, but the timing was modulated by environmental factors, such as rain and wind (Herbert and Lewis 1999). Paterson et al. (1995), using direct observations from a hide, found that possum activity started after sunset, reached its peak between 23:00 and 02:30 h and then gradually decreased towards sunrise. Our long-term study revealed modulations as possums commenced activity later after sunset on shorter (and warmer) nights than on longer (and colder) ones. On long winter nights, higher temperatures delayed activity significantly, whereas, on short summer nights, temperature had little or no impact. Nights in summer were either too short to allow for much flexibility or, during summer, temperatures at sunset were generally above the optimum, and therefore, possums delayed activity as much as possible. As expected, higher cloud cover allowed possums to be active later in the night on colder nights as temperature dropped more slowly. At the same time on warm nights, possums were active earlier when the sky was overcast. A possible explanation is that warm nights already have a narrow window of activity and with the slower temperature decline; delaying activity might not have been worth the lost feeding opportunity.
Moonlight
Unlike the environmental factors discussed above, moonlight does not im**e on energy expenditure directly, and its cyclic changes are unrelated to season or the 24-h day–night cycle. One of the interesting findings of our study was that the terrestrial detection rate for possums did not change with moonlight intensity. This contrasts with several previous studies that showed that feeding activity increases during a new moon (Lennon James 1998) and that possums in open woodland are stationary for more time on brighter nights, which might indicate predator avoidance behaviour (MacLennan 1984). Eyre (2004) found that moonlight negatively affected detections of common brushtail possums, mountain brushtail possums (Trichosurus cunninghami) and some gliders (Petauroidea). Other studies found no effect of moon phase on brushtail possum activity in New South Wales (Pickett 1999) and koomal (Trichosurus vulpecula hypoleucus) detections in south-western Australia (Wayne et al. 2005), which is consistent with our results. Interestingly, Parisi (2011) found that in New Zealand, possum activity was the highest during full moon and decreased significantly around new moon, particularly under canopy cover.
Moon phase is not linearly related to moonlight intensity (e.g. when 50% of lunar disc is illuminated, its brightness is only around 8% of that of a full moon). Around full moon, small daily changes in lunar phase (1–2%) can translate into major changes in moon brightness (up to 40%), while around new moon, there is minimal change in actual brightness despite large changes in moon phase (Śmielak 2023). Also, moon phase does not account for position of the moon in the sky, and its visibility above the horizon and overall is a poor proxy for lunar illumination (Kyba et al. 2020). Brushtail possums are relatively small folivores with an energy-poor diet high in tannins, require a steady food intake and are unable to enter torpor. In temperate eucalypt forests, foliage comprises on average half of their diet and presents significant challenges in meeting energy needs due to the high concentration of digestion-inhibiting compounds found in leaves (Hume 1999). These challenges lead to digestive and metabolic adaptations; for instance, the mean retention time of food by arboreal marsupials is similar to that of large ruminant and fermenting herbivores, ranging between 37 and 100 h (Cork and Foley 1991). The main factor limiting energetic intake in possums feeding on leaves is not the availability of food, but rather the quality of it and the time needed to digest, absorb and detoxify it (Nugent et al. 2000; Edwards et al. 2010). To maximise intake and mitigate the impact of plant chemical defences, possums have to either feed longer or travel further so they can frequently switch diets (Wiggins et al. 2003, 2006; Marsh et al. 2006). In the absence of better food sources such as fruit or invertebrates (particularly at times of increased energetic need, detailed earlier), possums are unlikely to be able to significantly decrease activity for the multiple days required for a large change in moon phase. Therefore, a preference towards a particular moon phase may not be noticeable. However, temporal modifications of activity are evident not between but within nights.
We found that possum terrestrial activity was correlated with the time of the lunar noon. This effect was stronger on brighter nights, which indicates that common brushtail possums preferred higher moon illumination. Since possums spent approximately 16% of their time feeding and 30% travelling (MacLennan 1984), we hypothesise that they exhibited within-night resource selection and focused their locomotor activity in the part of the night with optimal moonlight levels.
We hypothesise that this preference for moonlit parts of the night indicates that higher illumination levels provide an advantage to possums (improved foraging efficiency and/or predator detection) that outweigh the increased risk of predation that would result from spending more time on the ground. Few studies have assessed the effects of on-the-ground moonlight intensity, rather than moon phase, on animal activity. Gilmore (2016) rightly pointed out that previous studies of possum ecology struggled to use biologically meaningful measures of moonlight exposure, which might explain the inconclusive results that have been reported, ranging from lunar phobia (Lennon James 1998; Linley et al. 2020) to lunar philia (Cowan and Clout 2000; Parisi 2011). In studies where artificial light was used to simulate moonlight, possums in captive trials perceived illuminated patches as less risky (Nersesian et al. 2012), but no such effect was found when tested on possums in the wild (Mella et al. 2014). However, all these studies, using either moon phase or artificial light, assumed constant light levels through the night. The only study undertaken where light levels were measured hourly through the night revealed that illumination, but not moon phase, affected activity of five nocturnal pest species in New Zealand (Gilmore 2016). Due to the short duration of the study (12 nights) and resultant small sample sizes, the effect of illumination on possums specifically could not be analysed and was therefore modelled for all five pest species combined and was negative, which could be a type II error. Pooling the species could have contributed to the negative result because of the different ecological niches occupied by the five pest species, which ranged in size from mice to feral cats. We argue that understanding the effect of moonlight on animal activity requires higher temporal resolution of data, and, therefore, lunar illumination should either be measured or estimated using appropriate models (Śmielak 2023).
Conclusions
Our study provides a valuable contribution towards understanding common brushtail possum biology at different temporal scales. Furthermore, our work greatly expands knowledge of the temporal niche of possums in Australia, an aspect that has not been studied in detail before either in possums or in nocturnal mammals in general, predominantly due to technical limitations. Our study presents a novel approach to analysing changes in activity, with a higher temporal resolution than has commonly been used. Time-stamped records of activity, combined with a custom model of moonlight intensity, allowed us to detect subtle modulations in terrestrial activity from night to night.
We analysed drivers of terrestrial possum activity at various temporal scales using an extensive camera trap dataset collected over multiple years, which permitted temporal replication. Both temperature and rainfall were identified as important drivers, with results suggesting hot rather than cold weather limited possum terrestrial activity. This is particularly important in the context of climate change and can contribute to better predictions of how the species will respond to rising temperature, extreme weather and other climatic events and the likelihood of reestablishment in hotter environments where possums are locally extinct.
In our study, possums exhibited a very strong diel pattern of terrestrial activity, with all but seven of the recorded detections being nocturnal. Possums adjusted timing of their activity based on the season (night length) while modulating it in response to temperature and changes in moonlight intensity. Besides seasonal changes, weather and lunar illumination levels clearly regulated patterns of on-ground locomotion and therefore detection. An adverse effect of one environmental factor was often mitigated by another one; for instance, rain reduced activity on cold but not on warm nights. This shows that the temporal niche of possums is more complex than previously thought.
Data availability
The raw data analysed during this study (camera trap detections) are included are as a supplementary material.
Code availability
R code to replicate the analysis is provided in a dedicated public data reposity (https://github.com/msmielak/Brushtail_possum_activity).
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Acknowledgements
We thank the Invasive Animals Co-operative Research Centre and the Australian Government for providing postgraduate research scholarships to M.K.S.
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Conceptualisation, M.K.S, G.B., G.K. and P.J.S.F.; methodology, M.K.S., G.B., G.K., K.V. and P.J.S.F.; investigation, M.K.S.; data curation, M.K.S.; data analysis, M.K.S.; funding acquisition, P.J.S.F., G.B., N.R. and K.V.; project administration, N.R., K.V. and G.B.; resources, P.J.S.F., G.B., K.V. and N.R.; software, M.K.S.; supervision, N.R., K.V., G.B., G.K. and P.J.S.F.; and writing, original draft preparation, M.K.S. All authors provided feedback on interpretation of the data, reviewed and edited the submitted draft and read and agreed to the published version of the manuscript.
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Śmielak, M.K., Ballard, G., Fleming, P.J.S. et al. Brushtail possum terrestrial activity patterns are driven by climatic conditions, breeding and moonlight intensity. Mamm Res 68, 547–560 (2023). https://doi.org/10.1007/s13364-023-00691-5
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DOI: https://doi.org/10.1007/s13364-023-00691-5