Abstract
This study aims to isolate and characterize a novel rhamnolipid producer within the recent bioremediation approaches for treating hydrocarbon-contaminated soils in Algeria. In this context, from a hydrocarbon-contaminated soil, a newly bacterium designated LGMS7 was screened and identified, belonged to the Pseudomonas genus, and was closely related to Pseudomonas mucidolens, with a 16S rRNA sequence similarity of 99.05%. This strain was found to use different hydrocarbons and oils as a sole carbon and energy source for growth. It showed a stable emulsification index E24 (%) of 66.66% ± 3.46 when growing in mineral salts medium (MSM) supplemented with 2% (v/v) glycerol after incubation for 6 days at 30 °C. Interestingly, it was also able to reduce the surface tension of the cell-free supernatant to around 30 ± 0.65 mN m−1 with a critical micelle concentration (CMC) of 800 mg l−1. It was found to be able to produce around 1260 ± 0.57 mg l−1 as the yield of rhamnolipid production. Its biosurfactant has demonstrated excellent stability against pH (pH 2.0–12.0), salinity (0–150 g l−1), and temperature (−20 to 121 °C). Based on various chromatographic and spectroscopic techniques (i.e., TLC, FTIR, 1H-NMR), it was found to belong to the glycolipid class (i.e., rhamnolipids). Taken altogether, the strain LGMS7 and its biosurfactant display interesting biotechnological capabilities for the bioremediation of hydrocarbon-contaminated sites. To the best of our knowledge, this is the first study that described the production of biosurfactants by Pseudomonas mucidolens species.
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Acknowledgements
Authors are grateful to the team of Technological Properties of Lactic Acid Bacteria from the Laboratory of Microorganism Biology and Biotechnology LBMB, Oran, for the help in biochemical characterization using the API 20 E gallery. We also thank Mr. Peter Thornton for polishing the manuscript. Special thanks to Dr. Isabella Gandolfi for the DNA sequencing support.
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AC: conceptualization, writing–reviewing and editing. Dr. AC: supervision, methodology, software, writing–reviewing and editing. Pr. FB and Pr. AF: visualization, writing–reviewing, investigation, and supervision.
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Chaida, A., Chebbi, A., Bensalah, F. et al. Isolation and characterization of a novel rhamnolipid producer Pseudomonas sp. LGMS7 from a highly contaminated site in Ain El Arbaa region of Ain Temouchent, Algeria. 3 Biotech 11, 200 (2021). https://doi.org/10.1007/s13205-021-02751-6
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DOI: https://doi.org/10.1007/s13205-021-02751-6