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Fabrication and Osteoblastic Adhesion Behavior of Regenerated Silk Fibroin/PLLA Nanofibrous Scaffold by Double Syringe Electrospinning

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Abstract

In this work, regenerated silk fibroin (SF)/poly-L-lactic acid (PLLA) composite electrospun fibers were successfully prepared by electrospinning method with single and double syringe systems. Furtherly, we applied simultaneous and alternative spinning when using double syringe systems. The morphologies of the obtained silk/PLLA hybrid nanofibers were characterized by scanning electron microscope (SEM). Fourier transform infrared spectroscopy (FTIR) showed that PLLA has been well incorporated into the SF electrospun fibers using double syringes. Furthermore, the tensile test results indicate the tensile strength and Young's modulus of the silk/PLLA hybrid nanofibers webs were improved by both single and double syringe system. Moreover, the cell adhesion ratios of simultaneous silk/PLLA nanofibers were much higher than that of alternative silk/PLLA nanofibers, and even higher than pure silk nanofibers. Therefore, simultaneous spinning using double syringe system is a potential method for fabrication of nanofiber scaffold.

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Acknowledgments

The work was supported by National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou 215123, China.

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  1. These authors contribute equally to this work.

Authors and Affiliations

  1. National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215021, China

    Menglin Zhu, **g Gu, Lili He, Faraz Khan Mahar & Kai Wei

  2. Nano Fusion Technology Research Lab, Division of Frontier Fibers, Institute for Fiber Engineering (IFES), Interdisciplinary Cluster for Cutting Edge Research (ICCER), Shinshu University, Nagano, 386-8567, Japan

    Icksoo Kim

Authors
  1. Menglin Zhu
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  2. **g Gu
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  3. Lili He
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  4. Faraz Khan Mahar
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  5. Icksoo Kim
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  6. Kai Wei
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Correspondence to Icksoo Kim or Kai Wei.

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Zhu, M., Gu, J., He, L. et al. Fabrication and Osteoblastic Adhesion Behavior of Regenerated Silk Fibroin/PLLA Nanofibrous Scaffold by Double Syringe Electrospinning. Fibers Polym 20, 1850–1856 (2019). https://doi.org/10.1007/s12221-019-1188-7

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  • DOI: https://doi.org/10.1007/s12221-019-1188-7

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