Abstract
In the world's flower trade, gladiolus (Gladiolus spp.) is ranked first among bulbous flowers and eighth among cut flowers, with more than 30,000 different cultivars being grown. Mass multiplication and commercialization are restricted by the traditional propagation methods. However, the large-scale proliferation and improvement of the gladiolus have been accomplished with the aid of plant tissue culture and other biotechnological techniques. The current review includes a thorough examination of the growth and development parameters required for successful in vitro gladiolus development as well as cormel formation. Moreover, focus is being given to various techniques and methods such as in vitro cytogenetic stability and modification of chromosome number, in vitro mutagenesis and selection of pest resistance, in vitro identification and selection to develop virus-free germplasm, cryopreservation, synthetic seed technology, identifying virus diseases by RT-PCR, somaclonal variation, and protoplast and somatic hybridization. Molecular markers and their applications for genetic diversity analysis, relationships between different genotypes, and clonal stability analysis in Gladiolus species have been conducted by several research groups worldwide and are also being discussed. The article also covers efforts to enhance the functionality of plant phenotypes through genetic transformation. Future prospects for further improvement of ornamental gladiolus are also explored. Overall, the current review provides insight into the applications of basic and advanced biotechnological tools for gladiolus improvement.
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References
Manning, J., & Goldblatt, P. (2008). The iris family: Natural history and classification (pp. 138–142). Timber Press.
Cantor, M., Butta, E., Cristea, G., & Chis, L. M. (2010). Improvement of gladiolus varietal collection in order to use as genitors in breeding work. Bulletin UASVM Horticulture, 67, 1843–5394.
Kispotta, L. M., Jha, K. K., Horo, P., Tirkey, S. K., Misra, S., & Sengupta, S. (2017). Studies on combining ability and heterosis in Gladiolus (Gladiolus hybridus). International Journal of Environmental Science and Technology, 6, 420–442. https://doi.org/10.1080/14620316.2021.1990802
Kispotta, L. M., Jha, K. K., Horo, P., Tirkey, S. K., Misra, S., & Sengupta, S. (2017). Genetic variability and heritability in Gladiolus hybridus. International Journal of Environmental Science and Technology, 6, 519–528.
Pragya, P., Bhat, K. V., Misra, R. L., & Ranjan, J. K. (2010). Analysis of diversity and relation-ships among gladiolus cultivars using morphological and RAPD markers. Indian Journal of Agricultural Sciences, 80, 766–772.
Cantor, M., & Gladiolus, T. J. (2011). Wild crop relatives: Genomic and breeding resources (pp. 133–159). Springer.
Valente, L., Savolainen, V., Manning, J. C., Goldblatt, P., & Vargas, P. (2011). Explaining disparities in species richness between Mediterranean floristic regions: A case study in gladiolus (Iridaceae). Global Ecology and Biogeography, 20, 881–892. https://doi.org/10.1111/j.1466-8238.2010.00644.x
Anderson, N. O., Frick, J., Younis, A., & Currey, C. (2012). Heritability of cold tolerance (winter hardiness) Gladiolus grandiflorus. Plant Breed (pp. 298–312). InTech.
Datta, S. K. (2020). Breeding of Ornamentals: Gladiolus. International Journal of Life Sciences, 9, 115–133. https://doi.org/10.5958/2319-1198.2020.00007.X
Larson, R. A. (2012). Introduction to floriculture. Elsevier.
Ohri, D. (2013). Cytogenetics of domestication and improvement of garden gladiolus and bougainvillea. Nucleus, 56, 149–153. https://doi.org/10.1007/s13237-013-0091-7
Manzoor, A., Ahmad, T., Bashir, M. A., Baig, M. M. Q., Quresh, A. A., Shah, M. K. N., & Hafiz, I. A. (2018). Induction and identification of colchicine induced polyploidy in Gladiolus grandiflorus‘White Prosperity.’ Folia Horticulturae, 30, 307–319. https://doi.org/10.2478/fhort-2018-0026
Kadam, J. J., Agale, R. C., Rite, S. C., & Pandav, S. M. (2014). Exploration of fungicides and phyto-extract against Fusarium Oxysporum f. sp. gladioli causing corm rot of gladiolus. Discover Agriculture, 2, 61–64.
Chaudhary, V., Kumar, M., Sharma, S., Kumar, N., Kumar, V., Yadav, H. K., Sharma, S., & Sirohi, U. (2018). Assessment of genetic diversity and population structure in gladiolus (Gladiolus hybridus Hort.) by ISSR markers. Physiology and Molecular Biology of Plants, 24, 493–501. https://doi.org/10.1007/s12298-018-0519-2
Thorpe, T. A. (2007). History of plant tissue culture. Molecular Biotechnology, 37, 169–180. https://doi.org/10.1007/s12033-007-0031-3
Kumar, M., Sirohi, U., Malik, S., Kumar, S., Ahirwar, G. K., Chaudhary, V., Yadav, M. K., Singh, J., Kumar, A., & Pal, V. (2022). Prakash S Methods and factors influencing in vitro propagation efficiency of ornamental tuberose (Polianthes species): A systematic review of recent developments and future prospects. Horticulturae, 8, 998. https://doi.org/10.3390/horticulturae8110998
Mehbub, H., Akter, A., Akter, M. A., Mandal, M. S. H., Hoque, M. A., Tuleja, M., & Mehraj, H. (2022). Tissue culture in ornamentals: Cultivation factors, propagation techniques, and its application. Plants, 11, 3208. https://doi.org/10.3390/plants11233208
Winter, P., & Kahl, G. (1995). Molecular marker technologies for plant improvement. World Journal of Microbiology & Biotechnolog, 11, 438–448. https://doi.org/10.1007/BF00364619
Henry, R. (1997). Molecular markers in plant improvement. In R. Susi (Ed.), Practical Applications of Plant Molecular Biology (pp. 99–132). Chapman & Hall.
Baird, G., Abbott, A., Ballard, R., Sosinski, B., & Rajapakse, S. (1997). DNA diagnostics in horticulture. In P. Gresshoff (Ed.), Current Topics in Plant Molecular Biology: Technology Transfer of Plant Biotechnology (pp. 111–130). CRC Press.
Collard, B. C. Y., & Mackill, D. J. (2008). Marker-assisted selection: An approach for precision plant breeding in the twenty-first century. Philosophical Transactions of the Royal Society B: Biological Sciences., 363, 557–572. https://doi.org/10.1098/rstb.2007.2170
Kamo, K., Blowers, A., Smith, F., Van Eck, J., & Lawson, R. (1995). Stable transformation of gladiolus using suspension cells and callus. Journal of the American Society for Horticultural Science, 120, 347–352. https://doi.org/10.21273/JASHS.120.2.347
Kamo, K., Blowers, A., Smith, F., & Van Eck, J. (1995). Stable transformation of gladiolus by particle gun bombardment of cormels. Plant Science, 110, 105–111. https://doi.org/10.1016/0168-9452(95)04195-Z
Memon, N., Qasim, M., Jaskani, M. J., Khooharo, A. A., Hussain, Z., & Ahmad, I. (2013). Comparison of various explants on the basis of efficient shoot regeneration in gladiolus. Pakistan Journal of Botany, 45, 877–885.
Ascough, G. D., Erwin, J. E., & Van Staden, J. (2009). Micropropagation of Iridaceae—a review. PCTOC, 97, 1–19. https://doi.org/10.1007/s11240-009-9499-9
Kumar, A., Sood, A., Palni, L. M. S., & Gupta, A. K. (1999). In vitro propagation of Gladiolus hybridus Hort.: Synergistic effect of heat shock and sucrose onmorphogenesis. PCTOC, 57, 105–112. https://doi.org/10.1023/A:1006373314814
Memon, N., Qasim, M., Jaskani, M. J., Awan, F. S., Khan, A. I., Sadia, B., & Hussain, Z. (2012). Assessment of somaclonal variation in in-vitro propagated cormels of gladiolus. Pakistan Journal of Botany, 44, 769–776.
Kundang, N., Kumar, V., & Kumar, S. (2017). Effect of macronutrients and plant growth hormones for the in vitro formation of cormlets of Gladiolus Pacifica. CJB, 6, 10–16.
Kumar, A., Kumar, A., Sharma, V., Mishra, A., Singh, S., & Kumar, P. (2018). In vitro regeneration of gladiolus (Gladiolus hybrida L.): Optimization of growth media and assessment of genetic fidelity. International Journal of Current Microbiology and Applied Sciences, 7, 2900–2909. https://doi.org/10.20546/ijcmas.2018.710.337
Bajaj, Y. P. S., Sidhu, M. M. S., & Gill, A. P. S. (1983). Some factors affecting the in vitro propagation of Gladiolus. Scientia Horticulturae, 18, 269–275. https://doi.org/10.1016/0304-4238(83)90031-6
Singh, S. K., Misra, R. L., & Ranjan, J. K. (2012). In vitro shoot regeneration from cormel derived callus of gladiolus and bio-hardening of plantlets. Indian Journal of Biotechnology, 11, 99–104.
Saha, B., Datta, A. K., Datta, S., & da Silva, J. A. T. (2013). In vitro corm development, field evaluation and determination of genetic stability of corm-derived elite gladiolus germplasm. Floriculture and Ornamental Biotech, 7, 82–85.
Kabir, M. H., Mamun, A. N. K., Yesmin, F., & Subramaniam, S. (2014). In vitro propagation of Gladiolus dalenii from the callus through the culture of corm slices. Journal of Phytology, 6, 40–45.
Simonsen, J., & Hildebrandt, A. C. (1971). In vitro growth and differentiation of Gladiolus plants from callus cultures. Canadian Journal of Botany, 49, 1817–1819. https://doi.org/10.1139/b71-256
Kamo, K. (1995). A cultivar comperision of plant regeneration from suspension cells, callus, and cormel slices of gladiolus. In Vitro Cellular & Developmental Biology - Plant, 31, 113–115. https://doi.org/10.1007/BF02632247
Rernotti, P. C. (1995). Primary and secondary embryogenesis from cell suspension cultures of Gladiolus. Plant Science, 107, 205–214. https://doi.org/10.1016/0168-9452(95)04106-5
Kamo, K., Rajasekaran, K., & Cary, J. (2014). Growth characteristics of micropropagated, regenerated and transgenic gladiolus plants. Journal of Applied Horticulture, 16, 193–198.
Isah, T., Qurratul, & Umar, S. (2022). Influence of silver nitrate and copper sulphate on somatic embryogenesis, shoot morphogenesis, multiplication and associated physiological biochemical changes in Gladiolus hybridus L. PCTOC, 149, 563–587. https://doi.org/10.1007/s11240-022-02309-1
Hussain, I., Muhammad, A., Rashid, H., & Quraishi, A. (2001). In vitro multiplication of Gladiolus (Gladiolus crassifolius). Plant tissue culture, 11, 121–126.
Pathania, N. S., Misra, R. L., & Raghava, S. P. S. (2001). Precocious shoot proliferation and microcorm production in gladiolus through tissue culture. Journal of Ornamental Horticulture, 4, 69–73.
Roy, S. K., Gangopadhyay, G., Bandyopadhyay, T., Modak, B. K., Datta, S., & Mukherjee, K. K. (2006). Enhancement of in vitro micro corm production in gladiolus using alternative matrix. African Journal of Biotechnology, 5, 1204–1209.
Memon, N., Qasim, M., Jaskani, M. J., & Ahmad, R. (2010). In vitrocormel production of gladiolus. Pakistan Journal of Agricultural Sciences, 47, 115–123.
Priyakumari, I., & Sheela, V. L. (2005). Micropropagation of gladiolus cv. ‘PeachBlossom’through enhanced release axillary buds. Journal of Tropical Agriculture., 43, 47–50.
Prasad, V. S. S., & Gupta, S. D. (2006). In vitro shoot regeneration of gladiolus in semi-solid agar versus liquid cultures with support systems. PCTOC, 87, 263–271. https://doi.org/10.1007/s11240-006-9160-9
Aftab, F., Alam, M., Afrasiab, H., & Afrasiab, H. (2008). In vitro shoot multiplication and callus induction in Gladiolus hybridus Hort. Pakistan Journal of Botany, 40, 517–522.
Sheena, A., & Sheela, V. L. (2010). Effects of the growth retardant triadimefon on the ex-vitro establishment of gladiolus (Gladiolus grandifloras L.) cv. Vinks Glory. Plant Tissue Culture and Biotechnology, 20, 171–178. https://doi.org/10.3329/ptcb.v20i2.6896
Deshmukh, V. D., Kharde, A. V., & Talekar, B. K. (2021). Interactive effects of BA and IAA on shoot proliferation of gladiolus (gladiolus grandiflorus) var. white prosperity. THE Journal of Oriental Research Madras., 92, 12–19.
Sirohi, U., Kumar, M., Sharma, V. R., Teotia, S., Singh, D., Chaudhary, V., Sirohi, U., Priya, & Singh, M. K. (2022). CRISPR/Cas9 System: A potential tool for genetic improvement in floricultural crops. Molecular Biotechnology. https://doi.org/10.1007/s12033-022-00523-y
Emek, Y., & Erdag, B. (2007). In vitro propagation of Gladiolus anatolicus (Boiss.) Stapf. Pakistan Journal of Botany, 39, 23–30.
Sinha, P., & Roy, S. K. (2002). Plant regeneration through in vitro cormel formation from callus culture of Gladiolus primulinus Baker. Plant Cell, Tissue and Organ Culture, 12, 139–145.
Nezamabad, P. S., Habibi, M. K., Dizadji, A., & Kalantari, S. (2015). Elimination of bean yellow mosaic virus through thermotherapy combined with meristem-tip culture in gladiolus corms. Journal of Crop Protection, 4, 533–543. https://doi.org/10.1007/s13205-019-1684-x
Wu, J., Liu, C., Seng, S., Khan, M. A., Sui, J., Gong, B., Liu, C., Wu, C., Zhong, X., He, J., & Yi, M. (2015). Somatic embryogenesis and Agrobacterium-mediated transformation of Gladiolus hybridus cv. ‘Advance Red.’ PCTOC, 120, 717–728. https://doi.org/10.1007/s11240-014-0639-5
Mujib, A., Ali, M., Tonk, D., & Zafar, N. (2017). Nuclear 2C DNA and genome size analysis in somatic embryo regenerated gladiolus plants using flow cytometry. Advances in Horticultural Science, 31, 165–174.
Nishihara, M., Higuchi, A., Watanabe, A., & Tasaki, K. (2018). Application of the CRISPR/Cas9 system for modification of flower color in Torenia fournieri. BMC Plant Biology, 18, 1–9. https://doi.org/10.1186/s12870-018-1539-3
Choudhary, D., Agarwal, G., Singh, V. P., & Arora, A. (2010). In vitro micropropagation of Gladiolus grandiflora (var. Snow Princess) flower from cormel explant. Indian Journal of Plant Physiology, 15, 90–93.
Cai-hua, L., **-**, F., Shu-fang, G., & Dai-di, C. (2012). Study on mutant induction of gladiolusbyin vitro culture of petals. Journal of Northeast Agricultural University, 19, 38–42. https://doi.org/10.1016/S1006-8104(13)60020-3
Memon, N., Jasakni, M. J., Qasim, M., & Sharif, N. (2014). Cormel formation in gladiolus through tissue culture. Pakistan Journal of Agricultural Sciences, 51, 475–482.
El-Kazzaz, A. A., El-Bagoury, H., Mahmoud, I., El Tantawy, A. A., & Al-Ansary, A. A. (2012). Production of gladiolus germplasm via in vitro tissue cultures. JDU, 15, 440–447.
Haouala, F., & Chaieb, E. (2012). Effects of explant position and polarity on callus induction and shoot regeneration of gladiolus (Gladiolus hybridus Hort.). Floriculture Ornamental Biotech., 6, 133–139.
Zaidi, N., Khan, N. H., Zafar, F., & Zafar, S. I. (2000). Bulbous and cormous monocotyledonous ornamental plants in vitro. Science Vision, 6, 58–73.
Ziv, M., & Lilien-Kipnis, H. (2000). Bud regeneration from inflorescence explants for rapid propagation of geophytes in vitro. Plant Cell Reports, 19, 845–885. https://doi.org/10.1007/s002990000204
Cardoso, J. C., & Teixeira Silva, J. A. (2012). Micropropagation of gerbera using chlorine dioxide (ClO2) to sterilize the culture medium. In Vitro Cellular & Developmental Biology - Plant., 48, 362–368. https://doi.org/10.1007/s11627-011-9418-8
Belanekar, S. B., Nadkarni, H. R., Sawant, S. S., & Gokhale, N. B. (2010). Micropropagation in Gladiolus (Gladiolus grandiflorus L.) var. White Friendship. Journal of Maharashtra Agricultural University, 35, 66–68.
Cerasela, P., Giancarla, V., Camen, D., Andreea, P., Eleonora, N., Alina, S., & Gorinoiu, G. (2018). Evaluation of somaclonal variation in Gladiolus grandifloras through molecular markers ISSR. Journal of Horticulture, Forestry and Biotechnology, 22, 100–104.
George, E. F., Hall, M. A., & De Klerk, G. J. (2008). Plant tissue culture procedure-background. Plant Propagation by Tissue Culture (pp. 1–28). Springer.
George, E. F., & Debergh, P. C. (2008). Micropropagation: uses and methods Plant. Propagation by Tissue Culture (pp. 29–64). Springer.
Mateen, R. M. (2019). Development and optimization of micro-propagation, in vitro methodology for gladiolus. BSR, 1, 21–36.
Kumar, A., & Palni, L. M. S. (2013). Changes in endogenous polyamines during in vitro cormlet formation in Gladiolus hybridus Hort. Scientia Horticulturae, 162, 260–264. https://doi.org/10.1016/j.scienta.2013.08.003
Bera, A. K., Maity, T. R., Samanta, A., Dolai, A., Saha, B., & Datta, S. (2015). Enhancement of in vitro corm production in gladiolus by periodically replacement of liquid media using coir matrix. Journal of Applied Horticulture, 17, 222–224.
González-Pérez, E., Juárez-Muñoz, J., Ayala-Garay, O. J., Yáñez-Morales, M., & De, J. (2014). Ex vitro acclimatization of gladiolusplantlets. Propagation of Ornamental Plants, 14, 125–132.
Dharmasena, P. A. I. U., Karunananda, D. P., & Eeswara, J. P. (2011). Effect of gibberellic acid (GA3) and sugar on in vitrocormlet formation, multiplication and ex vitro sprouting of Gladiolus hybrida variety Princess Lee. Journal of Sustainable Tropical Agricultural Research, 23, 1–10. https://doi.org/10.4038/tar.v23i1.4626
Jala, A. (2013). Potential of benzyl adenine, naphthalene acetic acid and sucrose concentration on growth, development, and regeneration of new shoot and cormelon gladiolus. ATEAS, 2, 277–285.
Malviya, R. K., Tripathi, M., Vidhyashankar, M., Patel, R., & Ahuja, A. (2018). Effect of different phytohormones on plant regeneration of gladiolus (Gladiolus hybridus Hort.) from cultured cormel. Asian Journal of Microbiology, Biotechnology and Environmental Sciences, 19, 155–165.
Tripathi, M. K., Malviya, R. K., Vidhyashankar, M., & Patel, R. P. (2017). Effect of plant growth regulators on in vitro morphogenesis in gladiolus (Gladiolus hybridus Hort.) from cultured corm slice. International Journal of Agricultural Technology, 13, 583–599.
Altan, F., Bürün, B., & Sahin, N. (2010). Fungal contaminants observed during micropropagation of Lilium candidum L. and the effect of chemotherapeutic substances applied after sterilization. AJB, 9(7), 991–995.
Da Silva, J. A. T., Winarto, B., Dobránszki, J., Cardoso, J. C., & Zeng, S. (2016). Tissue disinfection for preparation of culture. Folia Horticulturae, 28(1), 57–75.
Bhojwani, S. S., & Dantu, P. K. (2013). Micropropagation. In S. S. Bhojwani & P. K. Dantu (Eds.), Plant tissue culture: an introductory text (pp. 245–274). Springer.
Mihaljevic, I., Dugalic, K., Tomas, V., Viljevac, M., Pranjic, A., Cmelik, Z., Puskar, B., & Jurkovic, Z. (2013). In vitro sterilization procedures for micropropagation of ‘Oblacinska’ sour cherry. Journal of Agricultural Science, 58(2), 117–126.
Mngomba, S. A., Sileshi, G., Du Toit, E. S., & Akinnifesi, F. K. (2012). Efficacy and utilization of fungicides and other antibiotics for aseptic plant cultures, fungicides for plant and animal diseases. In D. Dhanasekaran (Ed.), fungicides for plant and animal diseases (pp. 245–254). InTech.
Onwubiko, N. C., Nkogho, C. S., Anyanwu, C. P., & Onyeishi, G. C. (2013). Effect of different concentration of sterilant and exposure time on sweet potato (Ipomoea batatas Lam) explants. International Journal of Current Microbiology and Applied Sciences, 2(8), 14–20.
Sugii, N. C. (2011). The establishment of axenic seed and embryo cultures of endangered Hawaiian plant species: special review of disinfection protocols. In Vitro Cellular & Developmental Biology - Plant, 47, 157–169.
Budiarto, K. (2009). In vitro regeneration of three gladiolus cultivars using cormel explants. JID, 10, 109–113. https://doi.org/10.19184/JID.V10I2.82
Sutter, E. G. (1986). Micropropagation of Ixia viridifoliaand a Gladiolus × Homoglossumhybrid. Scientia Horticulturae, 29, 181–189. https://doi.org/10.1016/0304-4238(86)90045-2
Nasir, I. A., & Riazuddin, S. (2008). In vitro selection for Fusarium wilt resistance in gladiolus. Journal of Integrative Plant Biology, 50, 601–612. https://doi.org/10.1111/j.1744-7909.2008.00656.x
Dutta Gupta, S., & Prasad, V. S. S. (2010). Shoot multiplication kinetics and hyperhydric status of regenerated shoots of gladiolus in agar-solidified and matrix-supported liquid cultures. Plant Biotechnology Reports, 4, 85–94. https://doi.org/10.1007/s11816-009-0124-5
**ao, Y., Niu, G., & Kozai, T. (2011). Development and application of photoautotrophic micropropagation plant system. PCTOC. https://doi.org/10.1007/s11240-010-9863-9
Ojha, A., Sharma, V., Sharma, V. N., & Rawat, A. (2010). Effect of different carbohydrates sources on the formation of cormlets of economic important plant: Gladiolus Pacifica. IJBB., 6, 485–491.
Rakosy-Tican, E., Bors, B., & Szatmari, A. (2012). In vitro culture and medium-term conservation of the rare wild species Gladiolus imbricatus. African Journal of Biotechnology, 11, 14703–14712. https://doi.org/10.5897/AJB12.784
Dogra, N., & Dhatt, K. K. (2017). In vitro production of cormels in Gladiolus hybridus through gamma rays. International Journal of Current Microbiology and Applied Sciences, 6, 1308–1318. https://doi.org/10.20546/ijcmas.2017.610.154
Mokshin, E. V., Lukatkin, A. S., & Teixeira da Silva, J. A. (2006). Micropropagation of selected cultivars of lilium and gladiolus. Floriculture Ornamental Biotechnology, 2, 533–539.
Ruffoni, B., Savona, M., & Barberini, S. (2012). Biotechnological support for the development of new Gladiolus Hybrids. Floriculture Ornamental Biotechnology, 6, 45–52.
Ibrahim, A. I. (1994). Effect of gelling agent and activated charcoal on the growth and development of Cordyline terminalis cultured in vitro. First Conference Ornamental Horticulture, 1, 55–67.
Pierik, R. L. M. (1987). In vitro propagation of higher plants Handbook Martinus. Nijhoff Publishers. https://doi.org/10.1007/978-94-011-5750-6
Aslam, F., Habib, S., & Naz, S. (2012). Effect of different phytohormones on plant regeneration of Amaryllis hippeastrum. Pakistan Journal of Science, 64, 54–58.
Torabi-Giglou, M., & Hajieghrari, B. (2008). In vitro study on regeneration of Gladiolus grandiflorus corm calli as affected by plant growth regulators. Pakistan Journal of Biological Sciences, 11, 1147–1154. https://doi.org/10.3923/pjbs.2008.1147.1151
Goo, D. H., Joung, H. Y., & Kim, K. W. (2003). Differentiation of gladiolus plantlets from callus and subsequent flowering. Acta Horticulturae, 620, 339–342. https://doi.org/10.17660/ActaHortic.2003.620.42
Memon, N., Wahocho, N. A., Miano, T. F., & Leghari, M. H. (2016). Propagation of Gladiolus corms and cormels: A review. AJB, 15(32), 1699–1710.
Ginzburg, C., & Ziv, M. (1973). Hormonal regulation of corm formation in gladiolus stolons grown in vitro. Annals of Botany, 37, 219–224.
Steinitz, B., & Lilien-Kipnis, H. (1989). Control of precocious gladiolus corm and cormel formation in liquid shake cultures. Journal of Plant Physiology, 135, 495–500.
Kumar, A., Palni, L. M. S., Sood, A., Sharma, M., Palni, U. T., & Gupta, A. K. (2002). Heat-shock induced somatic embryogenesis in callus cultures of gladiolus in the presence of high sucrose. The Journal of Horticultural Science & Biotechnology, 77, 73–78. https://doi.org/10.1080/14620316.2002.11511460
Akhare, A. A., Dhumale, D. B., Sakhare, S. B., & Ekta, S. (2008). In vitro establishment of gladiolus cv. White Friendship and Fidelio using axillary buds as explant. Asian Journal of Horticulture, 3, 149–152.
Dantu, P. K., & Bhojwan, S. S. (1992). In vitro propagation of gladiolus: Optimisation of conditions for shoot multiplication. Journal of Plant Biochemistry and Biotechnology, 1, 115–118. https://doi.org/10.1007/BF03262908
Dantu, P. K., & Bhojwani, S. S. (1995). In vitro corm formation and field evaluation of corm-derived plants of gladiolus. Scientia Horticulturae, 61, 115–129. https://doi.org/10.1016/0304-4238(94)00722-R
Ziv, M., Halevy, A. H., & Shilo, R. (1970). Organs and plantlets regeneration of gladiolus through tissue culture. Annals of Botany, 34, 671–676. https://doi.org/10.1093/oxfordjournals.aob.a084400
Logan, A. E., & Zettler, F. W. (1985). Rapid in vitro propagation of virus-indexed gladioli. Acta Horticulturae, 164, 169–180. https://doi.org/10.17660/ActaHortic.1985.164.18
Singh, S. K., Misra, R. L., & Ranjan, J. K. (2010). In vitro rooting of gladiolus microshoots. Progressive Horticulture, 42(2), 243–245.
Nhut, D. T., da Silva, J. A. T., Huyen, P. X., & Paek, K. Y. (2004). The importance of explant source on regeneration and micropropagation of Gladiolus by liquid shake culture. Scientia Horticulturae, 102(4), 407–414. https://doi.org/10.1016/j.scienta.2004.04.004
Kumar, A., Palni, L. M. S., & Sood, A. (2011). Factors affecting in vitro formation of cormlets in Gladiolus hybridus Hort. and their field performance. Acta physiologiae plantarum, 33, 509–515. https://doi.org/10.1007/s11738-010-0574-y
Thun, V., Byun, M. S., Goo, D. H., & Kim, K. W. (2008). Large-sized cormlet induction and BYMV removal through tissue culture in gladiolus. Horticulture, Environment, and Biotechnology, 49, 332–335.
Memon, N., Qasim, M., Jaskani, M. J., Ahmad, R., & Anwar, R. (2009). Effect of various corm sizes on the vegetative, floral and corm yield attributes of gladiolus. Pakistan Journal of Agricultural Sciences, 46, 13–19.
Rakesh, B., Sudheer, W. N., & Nagella, P. (2021). Role of polyamines in plant tissue culture: An overview. PCTOC, 145, 487–506. https://doi.org/10.1007/s11240-021-02029-y
Sherif, N. A., Franklin Benjamin, J. H., Senthil Kumar, T., & Rao, M. V. (2018). Somatic embryogenesis, acclimatization and genetic homogeneity assessment of regenerated plantlets of Anoectochilus elatus Lindl., an endangered terrestrial jewel orchid. PCTOC, 132, 303–316. https://doi.org/10.1007/s11240-017-1330-4
Preece, J. E., & Sutter, E. G. (1991). Acclimatization of micropropagated plants to the greenhouse and field. In P. C. Debergh & R. H. Zimmerman (Eds.), Micropropagation (pp. 71–93). Kluwer Academic Publishers.
Chandra, S., Bandopadhyay, R., Kumar, V., & Chandra, R. (2010). Acclimatization of tissue cultured plantlets: From laboratory to land. Biotechnology Letters, 32, 1199–1205. https://doi.org/10.1007/s10529-010-0290-0
Memon, N. (2012). In vitro propagation of gladiolus plantlets and cormels. JHSOP, 4, 280–291.
Hannweg, K., Watt, M. P., & Berjak, P. (1996). A simple method for the micropropagation of Bowiea volubilis from inflorescence explants. Botanical Bulletin of Academia Sinica, 37, 213–218.
Paek, K. Y., & Murthy, H. N. (2002). High frequency of bulblet regeneration from bulb scale sections of Fritillaria thunbergi. PCTOC, 68, 247–252.
Naik, P. K., & Nayak, S. (2005). Different modes of plant regeneration and factors affecting in vitro bulblet production in Ornithogalum virens. ScienceAsia, 31, 409–414.
Ziv, M., & Lilien-Kipnis, H. (1990). Gladiolus. In P. A. Ammirato, D. A. Evans, W. R. Shark, & Y. P. S. Bajaj (Eds.), Handbook of Plant Cell Culture (pp. 461–478). Mcgraw Hill Publishing Co.
Nagaraju, V., Bhowmik, G., & Parthasarathy, V. A. (2002). Effect of paclobutrazol and sucrose on in vitro cormel formation in gladiolus. Acta Botanica Croatica, 61(1), 27–33.
Shah, A. H., Thakur, P., Dhiman, S. R., & Sharma, P. (2022). Role of plant growth regulators in flower crops Chapter. Advances in Agricultural, Animal and Fisheries Sciences, 129.
Rai, C. K., Rana, M., & Sharma, L. (2023). Response of Plant Growth Substances and Bio-enhancer on the Enhancement of Bulblet Growth under the In-vivo Condition of Lilium. International Journal of Plant & Soil Science., 35(14), 57–68.
Bandeira, S. O., Gaspar, F., & Pagula, F. P. (2001). African ethnobotany and healthcare: Emphasis on Mozambique. Pharmaceutical Biology, 39(1), 70–73.
Ngoupaye, G. T., Ngo Bum, E., & Daniels, W. M. U. (2013). Antidepressant-like effects of the aqueous macerate of the bulb of Gladiolus dalenii Van Geel (Iridaceae) in a rat model of epilepsy-associated depression. BMC Complementary and Alternative Medicine. https://doi.org/10.1186/1472-6882-13-272
Ngoupaye, G. T., Bum, E. N., Taiwe, G. S., Moto, F. C. O., & Talla, E. (2014). Antidepressant properties of aqueous acerate from Gladiolus Dalenii corms. African Journal of Traditional, Complementary and Alternative Medicines, 11(1), 53–61.
Ngoupaye, G. T., Pahaye, D. B., Ngondi, J., Moto, F. C. O., & Bum, E. N. (2017). Gladiolus dalenii lyophilisate reverses scopolamine-induced amnesia and reduces oxidative stress in rat brain. Biomedicine & Pharmacotherapy, 91, 350–357.
Fotsing, D., Ngoupaye, G. T., Ouafo, A. C., Njapdounke, S. K. J., Kenneth, Y. A., & Ngo Bum, E. (2017). Effects of Gladiolus dalenii on the stress-induced behavioral, neurochemical, and reproductive changes in rats. Frontiers in Pharmacology, 8, 685.
Matraszek-Gawron, R., Chwil, M., Terlecka, P., & Skoczylas, M. M. (2019). Recent studies on anti-depressant bioactive substances in selected species from the genera Hemerocallis and Gladiolus: A systematic review. Pharmaceuticals., 12(4), 172.
El-Shanawany, M. A., Hassanean, H. A., Mohamed, M. H., & Nafady, A. M. (2009). A new oleanene triterpene from Gladiolus segetum Ker-Gawl. Natural Product Research, 23(7), 613–616.
Abdessemed, D., & Dibi, A. (2013). Secondary metabolite from Gladiolus segetum. Journal of Chemical and Pharmaceutical Research, 5(12), 939–941.
Kim, Y. B., Park, S. Y., & Park, C. H. (2016). Metabolomics of differently colored Gladiolus cultivars. Applied Biological Chemistry, 59, 597–607. https://doi.org/10.1007/s13765-016-0197-0
Wang, D. Y., Ye, Q., Zhang, G. L., & Li, B. G. (2003). Note: New anthraquinones from Gladiolus gandavensis. Journal of Asian Natural Products Research, 5(4), 297–301.
Al-Jabera, H. I., Al-Qudahb, M. A., Odehc, F. M., & Zargac, M. H. A. (2019). Two new 28-noroleanane type triterpenoids and other constituents from Gladiolus atroviolaceus growing wild in Jordan. JJC, 14(1), 11–16.
Raknim, T. (1990). Effect of colchicine on mutation of gladiolus in vitro. Thesis (M.Sc. in Agriculture) Kasetsart University, Kasetsart, Bangkok, Thailand.
Saha, B., Datta, S., Datta, A. K., & Da Silva, J. A. T. (2011). Assessment of biochemical and molecular diversity of five elite gladiolus varieties. Floriculture and Ornamental Biotech., 5, 64–67.
Kutlunina, N., Permyakova, M., & Belyaev, A. (2017). Genetic diversity and reproductive traits in triploid and tetraploid populations of Gladiolus tenuis (Iridaceae). Plant Systematics and Evolution, 303, 1–10. https://doi.org/10.1007/s00606-016-1347-x
Raycheva, T., Stoyanov, K., & Denev, I. (2011). Genetic diversity and molecular taxonomy study of three genera from Iridaceae family in the Bulgarian flora based on ISSR markers. Biotechnology and Biotechnological Equipment, 25, 2484–2488. https://doi.org/10.5504/BBEQ.2011.0075
Kumar, P., Kumar, M., Naresh, R. K., Kumar, N., Chaudhary, P., & Sharma, S. (2016). Evaluation of genetic diversity among gladiolus (Gladiolus hybridus Hort.) germplasm using ISSR markers. International Journal of Agricultural and Statistical Sciences, 12, 277–283.
Zahoor, A., Dhatt, K. K., & Nageena, N. (2017). Genetic diversity analysis and DNA fingerprinting of elite stock of gladiolus (Gladiolus hybridus Hort.) using ISSR markers. Applied Biological Research, 19, 290–298. https://doi.org/10.5958/0974-4517.2017.00042.8
Geeta, S. V., Shirol, A. M., Nishani, S., Shiragur, M., & Varuna, K. J. (2014). Assessing genetic diversity of gladiolus varieties using SRAPs markers. Research Journal of Agricultural Science, 5, 658–661.
Rashmi, R., Lakshmana Reddy, D. C., Chandrashekar, S. Y., & Sukanya, T. S. (2016). Evaluation of genetic diversity and relation-ships among gladiolus genotypes by using morphological traits and SRAP markers. Green Farming, 7, 1346–1351.
Rashmi, R., Lakshmanareddy, D. C., & Chandrashekar, S. Y. (2016). Assessing genetic diversity of gladiolus (Gladiolus hybridus L) genotypes using sequence related amplified polymorphism (SRAP) markers. Ecology, Environment and Conservation, 22, 151–156.
Singh, N., Meena, B., Pal, A. K., Roy, R. K., Tewari, S. K., Tamta, S., & Rana, T. S. (2017). Nucleotide diversity and phylogenetic relationship among gladiolus cultivars and related taxa of family Iridaceae. Journal of Genetics, 96, 135–145. https://doi.org/10.1007/s12041-017-0755-1
Rymer, P. D., Manning, J. C., Goldblatt, P., Powell, M. P., & Savolainen, V. (2010). Evidence of recent and continuous speciation in a biodiversity hotspot: A population genetic approach in southern African gladioli (Gladiolus; Iridaceae). Molecular Ecology, 19, 4765–4782. https://doi.org/10.1111/j.1365-294X.2010.04794.x
Krishna, H., Alizadeh, M., Singh, D., Singh, U., Chauhan, N., Eftekhari, M., & Sadh, R. K. (2016). Somaclonal variations and their applications in horticultural crops improvement. 3 Biotech, 6, 54. https://doi.org/10.1007/s13205-016-0389-7
Singh, B. R., Dubey, V., & Aminuddin,. (2007). Inhibition of mosaic disease of gladiolus caused by bean yellow mosaic and cucumber mosaic viruses by virazole. Scientia Horticulturae, 114, 54–58. https://doi.org/10.1016/j.scienta.2007.05.003
Kaur, C., Raj, R., Kumar, S., Purshottam, D. K., Agrawal, L., Chauhan, P. S., & Raj, S. K. (2019). Elimination of bean yellow mosaic virus from infected cormels of three cultivars of gladiolus using thermo, electro and chemotherapy. 3 Biotech., 9, 154. https://doi.org/10.1007/s13205-019-1684-x
Bhattarai, A., Sajeed, A., & Bhardwaj, S. V. (2014). Production of Cucumber Mosaic Virus (CMV) free gladiolus shoots by meristem tip culture. J. Mycopathol. Res., 52, 107–111.
Raj, S. K., Kumar, S., & Verma, D. K. (2011). First report on molecular detection and identification of tomato aspermy virus naturally occurring on gladiolus in India. Phytoparasitica, 39, 303–307. https://doi.org/10.1007/s12600-011-0145-9
Selvaraj, D. G., Pokorný, R., & Holkova, L. (2009). Comparative analysis of ELISA, one step RT-PCR and IC-RT-PCR for the detection of bean yellow mosaic virus in gladiolus. Communications in Agricultural and Applied Biological Sciences, 74, 853–859.
Kaur, C., Kumar, S., Raj, S. K., Chauhan, P. S., & Sharma, N. (2015). Characterization of a new isolate of bean yellow mosaic virus group-IV associated with mosaic disease of gladiolus in India. Journal of Plant Pathology & Microbiology, 6, 10. https://doi.org/10.4172/2157-7471.1000309
Kaur, C., Raj, R., Srivastava, A., Kumar, S., & Raj, S. K. (2018). Sequence analysis of six full-length bean yellow mosaic virus genomes reveals phylogenetic diversity in India strains, suggesting subdivision of phylogenetic group-IV. Archives of Virology, 163, 235–242. https://doi.org/10.1007/s00705-017-3609-5
Kamo, K., Jordan, R., Guaragna, M. A., Hsu, H. T., & Ueng, P. (2010). Resistance to cucumber mosaic virus in gladiolus plants transformed with either a defective replicase or coat protein subgroup II gene from cucumber mosaic virus. Plant Cell Reports, 29, 695–704. https://doi.org/10.1007/s00299-010-0855-3
Pathania, N. S., & Misra, R. L. (2003). In vitro mutagenesis studies in gladiolus for induction of resistance to Fusarium oxysporumfsp gladioli. Acta Horticulturae, 624, 487–494. https://doi.org/10.17660/ActaHortic.2003.624.67
Kanwar, R., Nath, A. K., & Sharma, D. R. (2003). Cellular selection and partial characterization of gladiolus cell lines resistant to culture filtrate of Fusarium wilt. Indian Journal of Plant Physiology, 8, 1–5.
Rao, T. M., Negi, S. S., & Swamy, R. D. (1991). Isolation of leaf mesophyll protoplasts in gladiolus. Indian Journal of Horticulture, 48, 79–82.
Taylor, W., Bhatti, S., Long, D., & Sauve, R. (2000). In vitro culture of gladiolus. SNA Research Conference., 45, 328–330.
Ara, H., Jaiswal, U., & Jaiswal, V. S. (2000). Synthetic seed: Prospects and limitations. Current Science, 78, 1438–1444.
Sharma, S., Shahzad, A., & Da Silva, J. A. T. (2013). Synseed technology- a complete synthesis. Biotechnology Advances, 31, 186–207. https://doi.org/10.1016/j.biotechadv.2012.09.007
Ratilal, P.H. (2009). Tissue culture plant and synthetic seed production of gladiolus variety Amarican Beauty. M.Sc. Thesis Department of Floriculture and Landsca** Department, Aspee College of Horticulture and Forestry Gujarat Agriculture University, Navsari.
Tsai, S., & Lin, C. (2012). Advantages and applications of cryopreservation in Fisheries Science. Brazilian Archives of Biology and Technology, 55, 425–433. https://doi.org/10.1590/S1516-89132012000300014
Yi, J. Y., Lee, G. A., Chung, J. W., Lee, S. Y., & Lim, K. B. (2013). Efficient cryopreservation of Lilium spp. shoot tips using droplet-vitrification. Plant Breeding and Biotechnology, 1, 131–136. https://doi.org/10.9787/PBB.2013.1.2.131
Pegg, D. E. (2007). Principles of Cryopreservation. In J. G. Day & G. N. Stacey (Eds.), Cryopreservation and Freeze-Drying Protocols Methods in molecular biology. (Vol. 368). Humana Press.
Bojic, S., Murray, A., Bentley, B. L., Spindler, R., Pawlik, P., Cordeiro, J. L., & De Magalhães, J. P. (2021). Winter is coming: The future of cryopreservation. BMC boil., 19(1), 1–20.
Geng, X., Qiu, J., & Okubo, H. (2013). Changes of carbohydrate content during Lilium and Gladiolus pollen cryopreservation. Grana, 52, 202–206.
Lakshman, D. K., Pandey, R., Kamo, K., Bauchan, G., & Mitra, A. (2012). Genetic transformation of Fusarium oxysporum f spgladioli with Agrobacterium to study pathogenesis in gladiolus. European Journal of Plant Pathology, 133, 729–738. https://doi.org/10.1007/s10658-012-9953-0
Babu, P., & Chawla, H. S. (2000). In vitro regeneration and Agrobacterium mediated transformation in gladiolus. The Journal of Horticultural Science & Biotechnology, 75, 400–404. https://doi.org/10.1080/14620316.2000.11511258
Zhong, X., Yuan, X., Wu, Z., Khan, M. A., Chen, J., Li, X., Gong, B., Zhao, Y., Wu, J., Wu, C., & Yi, M. (2014). Virus-induced gene silencing for comparative functional studies in Gladiolus hybridus. Plant Cell Reports, 33, 301–312. https://doi.org/10.1007/s00299-013-1530-2
Zhong, X., **, L., Lian, Q., Luo, X., Wu, Z., Seng, S., Yuan, X., & Yi, M. (2015). The NPR1 homolog GhNPR1 plays an important role in the defense response of Gladiolus hybridus. Plant Cell Reports, 34, 1063–1074. https://doi.org/10.1007/s00299-015-1765-1
Imanishi, H. (1989). Collected data of plant genetic resources. Gladiolus 1077–1080.
Ohri, D., & Khoshoo, T. N. (1985). Cytogenetics of garden gladiolus II. Variation in chromosome complement and meiotic system. Cytologia, 50, 213–31. https://doi.org/10.1508/cytologia.50.213
Suzuki, K., Takatsu, Y., Gonai, T., & Kasumi, M. (2005). Plant regeneration and chromosome doubling of wild Gladiolus species. Acta Horticulture, 673, 175–181.
Duraisamy, G. S., Pokorny, R., & Holkova, L. (2011). Possibility of bean yellow mosaic virus detection in gladiolus plants by different methods. Journal of Plant Diseases and Protection., 118, 2–6. https://doi.org/10.1007/BF03356374
Budiarto, K., & Rosario, T. L. (2020). Evaluation of culture media for in vitro conservation of gladiolus cultivars. AGRIVITA Journal of Agricultural Science, 42, 205–213. https://doi.org/10.17503/agrivita.v0i0.2314
Nasir, I. A., Jamal, A., Rahman, Z., & Husnain, T. (2012). Molecular analysis of gladiolus lines with improved resistance against fusarium wilt. Pakistan Journal of Botany, 44, 73–79.
Ranjan, P., Bhat, K. V., Misra, R. L., Singh, S. K., & Ranjan, J. K. (2010). Genetic relationships of gladiolus cultivars inferred from fluorescence based AFLP markers. Scientia Horticulturae, 123, 562–567. https://doi.org/10.1016/j.scienta.2009.11.013
Rymer, P. D., Johnson, S. D., & Savolainen, V. (2010). Pollinator behaviour and plant speciation: Can assortative mating and disruptive selection maintain distinct floral morphs in sympatry. New Phytologist, 188, 426–436. https://doi.org/10.1111/j.1469-8137.2010.03438.x
Shufang, G., Huijuan, F. U., & **gang, W. (2010). ISSR Analysis of M1 generation of Gladiolus hybridusHort treated by EMS. Journal of Northeast Agricultural University, 17, 22–26.
Valente, L. M., Manning, J. C., Goldblatt, P., & Vargas, P. (2012). Did pollination shifts drive diversification in Southern African Gladiolus? Evaluating the model of pollinator-driven speciation. The American Naturalist, 180, 83–98. https://doi.org/10.1086/666003
Malik, K., & Pal, K. (2014). Genetic divergence and relationship analysis among twenty-two populations of gladiolus cultivars by morphological and RAPD-PCR tool. International Journal of Educational Research, 1, 1–8.
Kumari, P., Manjunatha, T., Sane, R. A., Kumar, R., & Dhananjaya, M. V. (2016). Characterization of Fusarium wilt in resistant and susceptible gladiolus (Gladiolus spp) genotypes using DNA markers. Indian Journal of Agricultural Sciences, 86, 849–853.
Szczepaniak, M., Kamiński, R., Kuta, E., Słomka, A., Heise, W., & Cieślak, E. (2016). Natural hybridization between Gladiolus palustris and G imbricatus inferred from morphological, molecular and reproductive evidence. Preslia, 88, 137–161.
Singh, N., Pal, A. K., Roy, R. K., Tamta, S., & Rana, T. S. (2016). Assessment of genetic variation and population structure in indigenous gladiolus cultivars inferred from molecular markers. The Nucleus, 59, 235–244. https://doi.org/10.1007/s13237-016-0181-4
Singh, N., Pal, A. K., Meena, B., Roy, R. K., Tamta, S., & Rana, T. S. (2017). Development of ISSR-and RAPD-derived SCAR markers for identification of gladiolus germplasm. The Journal of Horticultural Science & Biotechnology, 92, 577–582. https://doi.org/10.1080/14620316.2017.1309995
Singh, N., Pal, A. K., Roy, R. K., Tamta, S., & Rana, T. S. (2017). Development of cpSSR markers for analysis of genetic diversity in gladiolus cultivars. Plant Gene, 10, 31–36. https://doi.org/10.1016/j.plgene.2017.05.003
Singh, N., Mahar, K. S., Verma, S., Meena, B., Roy, R. K., Tewari, S. K., Goel, A. K., & Rana, T. S. (2018). Molecular analysis of genetic variability and relation-ship among gladiolus cultivars. Indian Journal of Biotechnology, 17, 118–127.
Singh, N., Pal, A. K., Roy, R. K., Tamta, S., & Rana, T. S. (2018). Characterization of gladiolus germplasm using morphological, physiological, and molecular markers. Biochemical Genetics, 56, 128–148. https://doi.org/10.1007/s10528-017-9835-4
Daco, L., Maurice, T., Muller, S., Rossa, J., & Colling, G. (2019). Genetic status of the endangered plant species Gladiolus palustris in the Western part of its distribution area. Conservation Genetics, 20, 1339–1354. https://doi.org/10.1007/s10592-019-01213-0
Malkócs, T., Almerekova, S., Bereczki, J., Cservenka, J., Meglécz, E., & Sramkó, G. (2019). Isolation and characterization of 15 SSR loci for the endangered European tetraploid species Gladiolus palustris (Iridaceae). Applications in Plant Sciences, 7, e01245. https://doi.org/10.1002/aps3.1245
Li, G., & Quiros, C. (2001). Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: Its application to map** and gene tagging in Brassica. TAG. Theoretical and Applied Genetics., 103, 455–461. https://doi.org/10.1007/s001220100570
Yang, L., Fu, S., Khan, M. A., Zeng, W., & Fu, J. (2013). Molecular cloning and development of RAPD-SCAR markers for Dimocarpus longan variety authentication. Springer Plus., 2, 501. https://doi.org/10.1186/2193-1801-2-501
Kamo, K., Aebig, J., Guaragna, M. A., James, C., Hsu, H. T., & Jordan, R. (2012). Gladiolus plants transformed with single-chain variable fragment antibodies to cucumber mosaic virus. PCTOC, 110, 13–21. https://doi.org/10.1007/s11240-012-0124-y
Kamo, K., Kim, A. Y., Park, S. H., & Joung, Y. H. (2012). The 5′ UTR-intron of the gladiolus polyubiquitin promoter GUBQ1 enhances translation efficiency in gladiolus and arabidopsis. BMC Plant Biology, 12, 79. https://doi.org/10.1186/1471-2229-12-79
Kamo, K., Lakshman, D., Bauchan, G., Rajasekaran, K., Cary, J., & Jaynes, J. (2015). Expression of a synthetic antimicrobial peptide, D4E1, in gladiolus plants for resistance to Fusarium oxysporum f sp gladioli. PCTOC, 121, 459–467. https://doi.org/10.1007/s11240-015-0716-4
Wu, J., Seng, S., Sui, J., Vonapartis, E., Luo, X., Gong, B., Liu, C., Wu, C., Liu, C., Zhang, F., & He, J. (2015). Gladiolus hybridus ABSCISIC ACID INSENSITIVE 5 (GhABI5) is an important transcription factor in ABA signaling that can enhance Gladiolus corm dormancy and Arabidopsis seed dormancy. Frontiers in Plant Science, 6, 960. https://doi.org/10.3389/fpls.2015.00960
Wu, J., Seng, S., Carianopol, C., Sui, J., Yang, Q., Zhang, F., Jiang, H., He, J., & Yi, M. (2016). Cloning and characterization of a novel Gladiolus hybridus AFP family gene (GhAFP-like) related to corm dormancy. Biochemical and Biophysical Research Communications, 471, 198–204. https://doi.org/10.1016/j.bbrc.2016.01.146
Seng, S., Wu, J., Liang, J., Zhang, F., Yang, Q., He, J., & Yi, M. (2017). Silencing GhAGPL1 reduces the quality and quantity of corms and cormels in Gladiolus. Journal of the American Society for Horticultural Science, 142, 119–125. https://doi.org/10.21273/JASHS03944-16
Wu, J., Wu, W., Liang, J., **, Y., Gazzarrini, S., He, J., & Yi, M. (2019). GhTCP19 transcription factor regulates corm dormancy release by repressing GhNCED expression in gladiolus. Plant and Cell Physiology, 60, 52–62. https://doi.org/10.1093/pcp/pcy186
Seng, S., Wu, C., Wu, J., Zhong, X., He, J., & Yi, M. (2020). Silencing GhCOI1 in Gladiolus hybridus increases susceptibility to Alternaria brassicicola and impairs inducible defenses. PCTOC, 140, 69–81. https://doi.org/10.1007/s11240-019-01711-6
Kamo, K., Lakshman, D., Pandey, R., Guaragna, M. A., Okubara, P., Rajasekaran, K., Cary, J., & Jordan, R. (2016). Resistance to Fusarium oxysporum f sp gladioli in transgenic gladiolus plants expressing either a bacterial chloroperoxidase or fungal chitinase genes. PCTOC, 124, 541–553. https://doi.org/10.1007/s11240-015-0913-1
Kamo, K. (2008). Transgene expression for gladiolus plants grown outdoors and in greenhouse. Scientia Horticulturae, 117, 275–280. https://doi.org/10.1016/j.scienta.2008.04.008
Kamo, K., & Joung, H. Y. (2009). Long-term gus expression from gladiolus callus lines containing either a bar-uidA fusion gene or bar and uidA delivered on separate plasmids. PCTOC, 98, 263–272. https://doi.org/10.1007/s11240-009-9558-2
Kamo, K., Joung, Y. H., & Green, K. (2009). GUS expression in gladiolus plants controlled by two gladiolus ubiquitin promoters. Floriculture and Ornamental Biotechnolgy, 3, 10–14.
Sivaprakasam, G., Singh, V. P., & Arora, A. (2009). Physiological and molecular analysis of effect of spermine on senescing petals of gladiolus. Indian Journal of Plant Physiology, 14, 384–391.
Lian, Q. L., **n, H. B., Li, X. X., Zhong, X. H., Yin, Y. L., & Yi, M. F. (2011). Cloning, characterization and expression analysis of a 9-lipoxygenase gene in Gladiolus hybridus. Scientia Horticulturae, 130, 468–475. https://doi.org/10.1016/j.scienta.2011.07.016
Lian, Q. L., **n, H. B., Li, X. X., Zhong, X. H., Yin, Y. L., & Yi, M. F. (2012). Analysis of GhAOS expression characteristics in Gladiolus hybridus and overexpression in Arabidopsis. Scientia Agricultura Sinica, 45, 2923–2930.
Lian, Q. L., **n, H. B., Li, X. X., Zhong, X. H., Yin, Y. L., & Yi, M. F. (2013). Isolation, characterization and expression analysis of the genes—GhAOS, GhAOC and GhOPR3: Encoding the key enzymes involved in jasmonic acid biosynthesis in Gladiolus hybridus. Scientia Horticulturae, 154, 88–95. https://doi.org/10.1016/j.scienta.2013.02.004
Lian, Q. L., **n, H. B., Li, X. X., Zhong, X. H., Yin, Y. L., & Yi, M. F. (2014). Heterologous expression of gladiolus GhOPR3 enhances the abiotic stress resistance of Arabidopsis. Acta Horticulturae Sinica, 41, 498–508.
Luo, X., Yi, J., Zhong, X. H., Lian, Q. L., Khan, M. A., Cao, X., Li, X. X., Gao, M. W., Wu, J., Chen, J., & Yi, M. F. (2012). Cloning, characterization and expression analysis of key genes involved in ABA metabolism in gladiolus cormels during storage. Scientia Horticulturae, 143, 115–121. https://doi.org/10.1016/j.scienta.2012.06.016
Seng, S., Wu, J., Sui, J., Wu, C., Zhong, X., Liu, C., Liu, C., Gong, B., Zhang, F., He, J., & Yi, M. (2016). ADP-glucose pyrophosphorylase gene plays a key role in the quality of corm and yield of cormels in gladiolus. Biochemical and Biophysical Research Communications, 474, 206–212. https://doi.org/10.1016/j.bbrc.2016.04.103
Dwivedi, S. K., Arora, A., Singh, V. P., Sairam, R., & Bhattacharya, R. C. (2016). Effect of sodium nitroprusside on differential activity of antioxidants and expression of SAGs in relation to vase life of gladiolus cut flowers. Scientia Horticulturae, 210, 158–165. https://doi.org/10.1016/j.scienta.2016.07.024
Elansary, H. O. (2020). Tree bark phenols regulate the physiological and biochemical performance of Gladiolus flowers. Process, 8, 71. https://doi.org/10.3390/pr8010071
Wu, J., **, Y., Liu, C., Vonapartis, E., Liang, J., Wu, W., Gazzarrini, S., He, J., & Yi, M. (2019). GhNAC83 inhibits corm dormancy release by regulating ABA signaling and cytokinin biosynthesis in Gladiolus hybridus. Journal of Experimental Botany, 70, 1221–1237. https://doi.org/10.1093/jxb/ery428
Gupta, S. D., & Datta, S. (2003). Antioxidant enzyme activities during in vitro morphogenesis of gladiolus and the effect of application of antioxidants on plant regeneration. Biologia Plantarum, 2, 179–183. https://doi.org/10.1023/b:biop.0000022248.62869.c7
Devi, P., Kumar, P., Sengar, R. S., Yadav, M. K., Kumar, M., Singh, S. K., & Singh, S. (2019). In-vitro multiple shoots production from cormel shoot buds in gladiolus (Gladiolus hybrida). International Journal of Current Microbiology and Applied Sciences, 8, 1345–1350. https://doi.org/10.20546/ijcmas.2019.807.160
Ziv, M. (1991). Morphogenic pattern of plants in liquid medium in shaken flasks or large-scale bioreactor cultures. Israel Journal of Botany, 40, 145–153. https://doi.org/10.1080/0021213X.1991.10677187
Ziv, M., & Ariel, T. (1991). Bud proliferation and plant regeneration in liquid-cultured philodendron treated with ancymidol and paclobutrazol. Journal of Plant Growth Regulation, 10, 1–4. https://doi.org/10.1007/BF02279311
Ziv, M. (2005). Simple bioreactors for mass propagation of plants. PCTOC, 81, 277–285. https://doi.org/10.1007/s11240-004-6649-y
Ziv, M., Ronen, G., & Raviv, M. (1998). Proliferation of meristematic clusters in disposable presterilized plastic bioreactors for large-scale micropropagation of plants. In Vitro Cell Development Biological Plant, 34, 152–158. https://doi.org/10.1007/BF02822781
Watanabe, K., Oda-Yamamizo, C., Sage-Ono, K., Ohmiya, A., & Ono, M. (2018). Alteration of flower colour in Ipomoea nil through CRISPR/Cas9-mediated mutagenesis of carotenoid cleavage dioxygenase 4. Transgenic Research, 27, 25–38. https://doi.org/10.1007/s11248-017-0051-0
Yu, J., Tu, L., Subburaj, S., Bae, S., & Lee, G. J. (2020). Simultaneous targeting of duplicated genes in Petunia protoplasts for flower color modification via CRISPR-Cas9 ribonucleoproteins. Plant Cell Reports, 40, 1037–1045. https://doi.org/10.1007/s00299-020-02593-1
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The authors appreciate Directorate of Research Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, UP, India, for providing the facilities and services required to complete the review.
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Kumar, M., Sirohi, U., Yadav, M.K. et al. In Vitro Culture Technology and Advanced Biotechnology Tools Toward Improvement in Gladiolus (Gladiolus species): Present Scenario and Future Prospects. Mol Biotechnol (2023). https://doi.org/10.1007/s12033-023-00818-8
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DOI: https://doi.org/10.1007/s12033-023-00818-8